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The map label for this gene is brnQ

Identifier: 16079722

GI number: 16079722

Start: 2727160

End: 2728482

Strand: Reverse

Name: brnQ

Synonym: BSU26690

Alternate gene names: 16079722

Gene position: 2728482-2727160 (Counterclockwise)

Preceding gene: 16079723

Following gene: 255767616

Centisome position: 64.72

GC content: 39.08

Gene sequence:

>1323_bases
ATGTCAAAAAAAGTGTCTGCTTCCTATATCATCATTATAGGATTAATGCTATTTGCATTGTTTTTTGGAGCAGGAAATCT
CATTTTTCCGCCTATGCTTGGTCAATTGGCCGGGAAAAATGTATGGGTCGCTAATGCAGGTTTCTTAGTGACCGGAGTTG
GATTGCCATTACTGGCTATAACAGCATTTGTTTTTTCGGGTAAACAAAACTTACAGTCGTTAGCCAGCCGTGTTCACCCG
GTGTTTGGCATTGTATTTACGACGATTCTCTATTTGGCAATCGGGCCTTTTTTCGCTATCCCGAGATCAGGAAACGTATC
GTTTGAAATTGGGGTAAAACCTTTTTTATCAAATGATGCAAGCCCTGTCTCATTGATCATATTTACAATATTATTTTTCG
CATTGGCATGCTTGTTATCACTCAATCCTTCAAAAATCATTGATATAGTTGGTAAATTTTTGACGCCAATTAAGCTGACA
TTTATCGGATTGCTTGTAGCCGTAGCTCTTATTCGCCCAATTGGAACCATTCAAGCACCATCAAAAGGGTACACATCACA
AGCGTTTTTTAAAGGATTTCAGGAAGGATACCTCACTCTGGATGCCCTTGTAGCATTTGTTTTTGGAATTATCATCGTGA
ATGCATTGAAAGAACAAGGAGCTTCTACCAAAAAACAGCTGATAGTTGTTTGTGCAAAAGCAGCCGCGATTGCAGCTGTA
CTCTTAGCAGTTATGTATACAGCCCTTTCCTATATGGGGGCTTCCAGCGTTGAAGAACTGGGGATTTTAGAAAATGGAGC
AGAGGTTTTGGCTAAGGTTTCATCCTATTATTTTGGATCTTATGGTTCAATCTTATTGGGCTTAATGATTACAGTGGCGT
GTCTGACGACAAGTGTCGGGCTGATCACAGCGTGTTCTTCATTTTTTCATGAATTATTTCCGAACATTTCTTATAAAAAA
ATTGCTGTCGTTTTATCAGTGTTTAGTACGCTTGTCGCAAATATAGGATTAACCCAGCTCATTAAGGTGTCAATGCCTGT
CTTACTGACGATGTATCCTATCGCGATTTCCTTAATATTTTTAACGTTTTTGCATTCTGTATTTAAAGGGAAGACAGAAG
TGTATCAAGGCAGTTTATTATTCGCATTCATCATCAGCCTATTTGACGGTTTGAAGGCGGCTGGAATAAAAATTGAAGTT
GTGAATCGTATTTTCACTCAGATTCTTCCTATGTATAACATAGGGCTGGGGTGGCTCATTCCAGCTATTGCAGGAGGAAT
ATGCGGCTATATACTGAGTATATTCCGAACAAAAACAAGTTGA

Upstream 100 bases:

>100_bases
ACACAAGCTTATTTTGGAATCAACACTGTGCCAGTGCAAATAGCATCTGTAATGTTTCCTGGCTATTCAAGAAGTGTCTA
CTTTAATCGGAGGATATATA

Downstream 100 bases:

>100_bases
TCAATCAACAGCAATATCACTCAGATCTGCAGGATCTCCTCCATCAGTGGATACTTCAACTGTTACAATTGGATGTTCTG
CCCCTGTCCAACCGAAAATA

Product: low-affinity branched-chain amino acid transporter

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSKKVSASYIIIIGLMLFALFFGAGNLIFPPMLGQLAGKNVWVANAGFLVTGVGLPLLAITAFVFSGKQNLQSLASRVHP
VFGIVFTTILYLAIGPFFAIPRSGNVSFEIGVKPFLSNDASPVSLIIFTILFFALACLLSLNPSKIIDIVGKFLTPIKLT
FIGLLVAVALIRPIGTIQAPSKGYTSQAFFKGFQEGYLTLDALVAFVFGIIIVNALKEQGASTKKQLIVVCAKAAAIAAV
LLAVMYTALSYMGASSVEELGILENGAEVLAKVSSYYFGSYGSILLGLMITVACLTTSVGLITACSSFFHELFPNISYKK
IAVVLSVFSTLVANIGLTQLIKVSMPVLLTMYPIAISLIFLTFLHSVFKGKTEVYQGSLLFAFIISLFDGLKAAGIKIEV
VNRIFTQILPMYNIGLGWLIPAIAGGICGYILSIFRTKTS

Sequences:

>Translated_440_residues
MSKKVSASYIIIIGLMLFALFFGAGNLIFPPMLGQLAGKNVWVANAGFLVTGVGLPLLAITAFVFSGKQNLQSLASRVHP
VFGIVFTTILYLAIGPFFAIPRSGNVSFEIGVKPFLSNDASPVSLIIFTILFFALACLLSLNPSKIIDIVGKFLTPIKLT
FIGLLVAVALIRPIGTIQAPSKGYTSQAFFKGFQEGYLTLDALVAFVFGIIIVNALKEQGASTKKQLIVVCAKAAAIAAV
LLAVMYTALSYMGASSVEELGILENGAEVLAKVSSYYFGSYGSILLGLMITVACLTTSVGLITACSSFFHELFPNISYKK
IAVVLSVFSTLVANIGLTQLIKVSMPVLLTMYPIAISLIFLTFLHSVFKGKTEVYQGSLLFAFIISLFDGLKAAGIKIEV
VNRIFTQILPMYNIGLGWLIPAIAGGICGYILSIFRTKTS
>Mature_439_residues
SKKVSASYIIIIGLMLFALFFGAGNLIFPPMLGQLAGKNVWVANAGFLVTGVGLPLLAITAFVFSGKQNLQSLASRVHPV
FGIVFTTILYLAIGPFFAIPRSGNVSFEIGVKPFLSNDASPVSLIIFTILFFALACLLSLNPSKIIDIVGKFLTPIKLTF
IGLLVAVALIRPIGTIQAPSKGYTSQAFFKGFQEGYLTLDALVAFVFGIIIVNALKEQGASTKKQLIVVCAKAAAIAAVL
LAVMYTALSYMGASSVEELGILENGAEVLAKVSSYYFGSYGSILLGLMITVACLTTSVGLITACSSFFHELFPNISYKKI
AVVLSVFSTLVANIGLTQLIKVSMPVLLTMYPIAISLIFLTFLHSVFKGKTEVYQGSLLFAFIISLFDGLKAAGIKIEVV
NRIFTQILPMYNIGLGWLIPAIAGGICGYILSIFRTKTS

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family

Homologues:

Organism=Escherichia coli, GI1786601, Length=423, Percent_Identity=39.7163120567376, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BRNQ_BACSU (P94499)

Other databases:

- EMBL:   U79494
- EMBL:   U93876
- EMBL:   AL009126
- PIR:   D69596
- RefSeq:   NP_390546.1
- ProteinModelPortal:   P94499
- EnsemblBacteria:   EBBACT00000001612
- GeneID:   936930
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU26690
- NMPDR:   fig|224308.1.peg.2671
- GenoList:   BSU26690
- GeneTree:   EBGT00070000031951
- HOGENOM:   HBG533860
- OMA:   VAFNQIV
- PhylomeDB:   P94499
- ProtClustDB:   CLSK887636
- BioCyc:   BSUB:BSU26690-MONOMER
- InterPro:   IPR004685
- TIGRFAMs:   TIGR00796

Pfam domain/function: PF05525 Branch_AA_trans

EC number: NA

Molecular weight: Translated: 47007; Mature: 46876

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10e54eec)-; HASH(0x11365270)-; HASH(0x112c1e6c)-; HASH(0x1139c0f4)-; HASH(0x1139e868)-; HASH(0x1138e778)-; HASH(0x10bc8000)-; HASH(0x11380ecc)-; HASH(0x103e3ac4)-; HASH(0x112c1e84)-; HASH(0x113a0b34)-; HASH(0x10dd6b08)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
SKKVSASYIIIIGLMLFALFFGAGNLIFPPMLGQLAGKNVWVANAGFLVTGVGLPLLAI
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
TAFVFSGKQNLQSLASRVHPVFGIVFTTILYLAIGPFFAIPRSGNVSFEIGVKPFLSNDA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCC
SPVSLIIFTILFFALACLLSLNPSKIIDIVGKFLTPIKLTFIGLLVAVALIRPIGTIQAP
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SKGYTSQAFFKGFQEGYLTLDALVAFVFGIIIVNALKEQGASTKKQLIVVCAKAAAIAAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LLAVMYTALSYMGASSVEELGILENGAEVLAKVSSYYFGSYGSILLGLMITVACLTTSVG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LITACSSFFHELFPNISYKKIAVVLSVFSTLVANIGLTQLIKVSMPVLLTMYPIAISLIF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LTFLHSVFKGKTEVYQGSLLFAFIISLFDGLKAAGIKIEVVNRIFTQILPMYNIGLGWLI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHH
PAIAGGICGYILSIFRTKTS
HHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377