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The map label for this gene is blt

Identifier: 16079712

GI number: 16079712

Start: 2716973

End: 2718175

Strand: Direct

Name: blt

Synonym: BSU26590

Alternate gene names: 16079712

Gene position: 2716973-2718175 (Clockwise)

Preceding gene: 16079698

Following gene: 16079713

Centisome position: 64.45

GC content: 38.99

Gene sequence:

>1203_bases
ATGAAAAAATCAATAAATGAGCAAAAAACGATATTCATTATACTATTAAGCAACATCTTCGTAGCATTTCTTGGTATCGG
TTTAATCATTCCAGTTATGCCTTCTTTTATGAAAATCATGCATTTATCCGGCAGCACAATGGGTTATCTTGTTGCGGCTT
TTGCCATTTCTCAGTTAATTACTTCACCTTTTGCAGGTAGGTGGGTTGACCGTTTCGGGAGAAAAAAAATGATTATTCTC
GGGTTGCTTATATTCAGTTTATCTGAGTTGATTTTCGGATTAGGGACCCATGTTTCAATATTTTATTTCTCGAGGATATT
GGGTGGTGTAAGTGCGGCTTTTATCATGCCCGCGGTAACAGCATATGTAGCTGATATTACAACCCTAAAGGAAAGGTCAA
AGGCTATGGGGTATGTTTCTGCTGCAATTAGCACCGGCTTTATTATTGGACCTGGTGCGGGAGGATTTATTGCCGGCTTT
GGTATCCGCATGCCGTTTTTCTTCGCCTCCGCCATCGCGTTAATAGCAGCTGTCACTTCCGTTTTTATACTAAAAGAGTC
ATTGTCGATAGAAGAACGCCATCAACTCTCATCTCATACAAAGGAATCAAATTTCATTAAAGACTTGAAGAGATCCATTC
ATCCTGTCTATTTCATTGCATTTATTATCGTCTTTGTAATGGCTTTTGGTTTATCAGCTTATGAAACGGTATTCAGCTTG
TTTTCTGATCATAAATTTGGCTTCACACCAAAAGATATTGCAGCCATTATTACGATTAGTTCCATTGTTGCGGTAGTTAT
TCAAGTTTTACTATTCGGGAAATTGGTCAACAAACTTGGAGAGAAAAGAATGATTCAGCTGTGCTTAATAACCGGTGCGA
TCTTGGCTTTCGTGTCTACTGTTATGTCAGGATTTTTAACTGTTTTGCTTGTAACTTGTTTTATTTTTCTGGCGTTCGAT
TTGCTACGTCCGGCCTTAACCGCTCATTTATCCAATATGGCCGGTAACCAGCAGGGTTTCGTAGCAGGCATGAACTCCAC
ATACACCAGCCTGGGAAATATATTTGGACCTGCTCTAGGCGGTATACTATTTGATCTTAACATTCATTATCCTTTCCTTT
TTGCAGGTTTCGTTATGATTGTCGGCCTTGGTCTTACAATGGTTTGGAAAGAAAAAAAGAATGATGCTGCAGCTTTGAAT
TAA

Upstream 100 bases:

>100_bases
ATACGGTAACCATATACCTTATGATTTGATTGTACTTGAAAAAAGCTTCAAGCGAAAAGGATAGGTAAAAAGGGTTCAAT
TGAGTAAAAGGGGAATTCAG

Downstream 100 bases:

>100_bases
TTCATTTTCTATAAAGTTAAGTCATTTGGATGCAGGGAATGCCCCTGGTTAGGCACAATAAGAAAAGGTACTTAATTTGA
TAGGCTACATGTTGCTTGAC

Product: efflux transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 2

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKKSINEQKTIFIILLSNIFVAFLGIGLIIPVMPSFMKIMHLSGSTMGYLVAAFAISQLITSPFAGRWVDRFGRKKMIIL
GLLIFSLSELIFGLGTHVSIFYFSRILGGVSAAFIMPAVTAYVADITTLKERSKAMGYVSAAISTGFIIGPGAGGFIAGF
GIRMPFFFASAIALIAAVTSVFILKESLSIEERHQLSSHTKESNFIKDLKRSIHPVYFIAFIIVFVMAFGLSAYETVFSL
FSDHKFGFTPKDIAAIITISSIVAVVIQVLLFGKLVNKLGEKRMIQLCLITGAILAFVSTVMSGFLTVLLVTCFIFLAFD
LLRPALTAHLSNMAGNQQGFVAGMNSTYTSLGNIFGPALGGILFDLNIHYPFLFAGFVMIVGLGLTMVWKEKKNDAAALN

Sequences:

>Translated_400_residues
MKKSINEQKTIFIILLSNIFVAFLGIGLIIPVMPSFMKIMHLSGSTMGYLVAAFAISQLITSPFAGRWVDRFGRKKMIIL
GLLIFSLSELIFGLGTHVSIFYFSRILGGVSAAFIMPAVTAYVADITTLKERSKAMGYVSAAISTGFIIGPGAGGFIAGF
GIRMPFFFASAIALIAAVTSVFILKESLSIEERHQLSSHTKESNFIKDLKRSIHPVYFIAFIIVFVMAFGLSAYETVFSL
FSDHKFGFTPKDIAAIITISSIVAVVIQVLLFGKLVNKLGEKRMIQLCLITGAILAFVSTVMSGFLTVLLVTCFIFLAFD
LLRPALTAHLSNMAGNQQGFVAGMNSTYTSLGNIFGPALGGILFDLNIHYPFLFAGFVMIVGLGLTMVWKEKKNDAAALN
>Mature_400_residues
MKKSINEQKTIFIILLSNIFVAFLGIGLIIPVMPSFMKIMHLSGSTMGYLVAAFAISQLITSPFAGRWVDRFGRKKMIIL
GLLIFSLSELIFGLGTHVSIFYFSRILGGVSAAFIMPAVTAYVADITTLKERSKAMGYVSAAISTGFIIGPGAGGFIAGF
GIRMPFFFASAIALIAAVTSVFILKESLSIEERHQLSSHTKESNFIKDLKRSIHPVYFIAFIIVFVMAFGLSAYETVFSL
FSDHKFGFTPKDIAAIITISSIVAVVIQVLLFGKLVNKLGEKRMIQLCLITGAILAFVSTVMSGFLTVLLVTCFIFLAFD
LLRPALTAHLSNMAGNQQGFVAGMNSTYTSLGNIFGPALGGILFDLNIHYPFLFAGFVMIVGLGLTMVWKEKKNDAAALN

Specific function: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally div

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family

Homologues:

Organism=Homo sapiens, GI39753965, Length=319, Percent_Identity=26.9592476489028, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI217416366, Length=390, Percent_Identity=21.5384615384615, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787291, Length=364, Percent_Identity=25, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71999703, Length=229, Percent_Identity=24.8908296943231, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17551570, Length=200, Percent_Identity=27, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71996331, Length=330, Percent_Identity=25.7575757575758, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17738085, Length=353, Percent_Identity=24.929178470255, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI17137160, Length=369, Percent_Identity=22.7642276422764, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357569, Length=367, Percent_Identity=26.4305177111717, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI62471713, Length=270, Percent_Identity=27.7777777777778, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI62471717, Length=270, Percent_Identity=27.7777777777778, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI62471715, Length=270, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24656463, Length=370, Percent_Identity=26.2162162162162, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24656468, Length=370, Percent_Identity=26.2162162162162, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI161080926, Length=370, Percent_Identity=26.2162162162162, Blast_Score=65, Evalue=1e-10,
Organism=Drosophila melanogaster, GI161080924, Length=370, Percent_Identity=26.2162162162162, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BMR2_BACSU (P39843)

Other databases:

- EMBL:   L32599
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   I39792
- RefSeq:   NP_390536.1
- ProteinModelPortal:   P39843
- EnsemblBacteria:   EBBACT00000002734
- GeneID:   937646
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU26590
- NMPDR:   fig|224308.1.peg.2661
- GenoList:   BSU26590
- GeneTree:   EBGT00070000031981
- HOGENOM:   HBG710362
- OMA:   IFAIVCI
- ProtClustDB:   CLSK887542
- BioCyc:   BSUB:BSU26590-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958
- PRINTS:   PR01035
- TIGRFAMs:   TIGR00880

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 43425; Mature: 43425

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50850 MFS; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x106db7e4)-; HASH(0x1122d918)-; HASH(0x111be62c)-; HASH(0x101fa00c)-; HASH(0x112ce938)-; HASH(0x10e59324)-; HASH(0x10403d38)-; HASH(0x111da610)-; HASH(0x111c0d0c)-; HASH(0x10e61398)-; HASH(0x11148510)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
>Mature Secondary Structure
MKKSINEQKTIFIILLSNIFVAFLGIGLIIPVMPSFMKIMHLSGSTMGYLVAAFAISQLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TSPFAGRWVDRFGRKKMIILGLLIFSLSELIFGLGTHVSIFYFSRILGGVSAAFIMPAVT
HCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AYVADITTLKERSKAMGYVSAAISTGFIIGPGAGGFIAGFGIRMPFFFASAIALIAAVTS
HHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VFILKESLSIEERHQLSSHTKESNFIKDLKRSIHPVYFIAFIIVFVMAFGLSAYETVFSL
HHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FSDHKFGFTPKDIAAIITISSIVAVVIQVLLFGKLVNKLGEKRMIQLCLITGAILAFVST
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMSGFLTVLLVTCFIFLAFDLLRPALTAHLSNMAGNQQGFVAGMNSTYTSLGNIFGPALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHH
GILFDLNIHYPFLFAGFVMIVGLGLTMVWKEKKNDAAALN
HHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608059; 8969508; 9384377