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The map label for this gene is rimO

Identifier: 16079597

GI number: 16079597

Start: 2621677

End: 2623032

Strand: Reverse

Name: rimO

Synonym: BSU25430

Alternate gene names: 16079597

Gene position: 2623032-2621677 (Counterclockwise)

Preceding gene: 16079598

Following gene: 16079595

Centisome position: 62.22

GC content: 43.51

Gene sequence:

>1356_bases
ATGGCAACTGTTGCTTTCCATACGCTTGGCTGTAAAGTCAACCATTATGAAACAGAAGCAATCTGGCAGCTTTTCAAAGA
AGCGGGCTATGAAAGAAGAGACTTTGAACAAACAGCTGATGTATATGTCATTAATACATGTACGGTTACAAATACGGGAG
ATAAAAAAAGCCGCCAAGTGATCAGACGCGCCATTCGTCAAAATCCTGACGGTGTCATCTGTGTCACAGGCTGCTACGCG
CAAACCTCACCTGCTGAGATTATGGCGATCCCCGGTGTCGACATTGTAGTCGGAACACAGGACCGTGAAAAAATGCTTGG
CTACATTGACCAATACCGAGAAGAACGCCAGCCGATTAATGGCGTCAGCAACATCATGAAAGCAAGAGTATATGAAGAGC
TTGATGTGCCTGCTTTCACGGACCGCACAAGAGCGTCATTGAAAATACAGGAGGGCTGCAATAATTTCTGCACATTCTGT
ATCATTCCGTGGGCGAGAGGCCTGCTCCGCTCCCGCGACCCTGAAGAAGTCATTAAACAAGCGCAGCAGCTTGTCGATGC
CGGATATAAGGAAATTGTTCTTACGGGTATTCATACAGGCGGCTACGGCGAAGATATGAAAGATTACAACTTCGCGAAGC
TTTTGAGCGAATTAGACACCCGTGTGGAAGGCGTGAAACGGATCAGAATTTCTTCAATCGAAGCCAGCCAAATCACTGAC
GAAGTGATCGAGGTCTTAGACCGCTCAGATAAGATAGTGAATCATTTGCATATTCCTATCCAATCCGGATCAAACACAGT
GCTTAAACGGATGAGACGCAAATATACAATGGAATTCTTTGCAGACCGTTTAAATAAATTGAAAAAAGCACTGCCAGGCT
TAGCTGTTACTTCTGATGTCATCGTCGGGTTCCCTGGAGAAACAGAAGAAGAATTTATGGAAACCTATAACTTTATTAAA
GAACATAAATTCTCTGAGCTGCATGTTTTCCCTTACAGTAAGCGTACAGGAACACCAGCTGCACGAATGGAAGACCAAGT
GGATGAAAACGTGAAAAACGAACGTGTGCACCGCCTGATTGCTCTTTCTGACCAGCTTGCAAAAGAATACGCTTCTCAGT
ATGAAAATGAAGTGCTTGAGATTATCCCTGAGGAAGCGTTTAAGGAAACAGAAGAAGAAAATATGTTTGTCGGTTACACA
GACAATTATATGAAGGTTGTTTTCAAAGGTACGGAAGATATGATCGGCAAAATCGTAAAAGTCAAAATCCTAAAAGCAGG
TTATCCTTACAACGAAGGACAGTTTGTCCGTGTGGTTGAGGATGAAATAACTGAACACATGCGTTTGTCTTCTTAA

Upstream 100 bases:

>100_bases
CGTGCGGCCTTGGACCGAGAATTTTAAGGACAGAAACCGCTCCGCTATACGCGTTGAGTGCGATTTCTTATCAAACAGAG
TTATTAAGAGGTGATCAGTA

Downstream 100 bases:

>100_bases
CCTAAAAAATCGGTGCATTAAAATGTACCGATTTTTTTATTTAGCCGGGATAAGAAGGTGAATCAATCGGTAAAACAAGT
TTGTAAGCGGAAGAAATAAA

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MATVAFHTLGCKVNHYETEAIWQLFKEAGYERRDFEQTADVYVINTCTVTNTGDKKSRQVIRRAIRQNPDGVICVTGCYA
QTSPAEIMAIPGVDIVVGTQDREKMLGYIDQYREERQPINGVSNIMKARVYEELDVPAFTDRTRASLKIQEGCNNFCTFC
IIPWARGLLRSRDPEEVIKQAQQLVDAGYKEIVLTGIHTGGYGEDMKDYNFAKLLSELDTRVEGVKRIRISSIEASQITD
EVIEVLDRSDKIVNHLHIPIQSGSNTVLKRMRRKYTMEFFADRLNKLKKALPGLAVTSDVIVGFPGETEEEFMETYNFIK
EHKFSELHVFPYSKRTGTPAARMEDQVDENVKNERVHRLIALSDQLAKEYASQYENEVLEIIPEEAFKETEEENMFVGYT
DNYMKVVFKGTEDMIGKIVKVKILKAGYPYNEGQFVRVVEDEITEHMRLSS

Sequences:

>Translated_451_residues
MATVAFHTLGCKVNHYETEAIWQLFKEAGYERRDFEQTADVYVINTCTVTNTGDKKSRQVIRRAIRQNPDGVICVTGCYA
QTSPAEIMAIPGVDIVVGTQDREKMLGYIDQYREERQPINGVSNIMKARVYEELDVPAFTDRTRASLKIQEGCNNFCTFC
IIPWARGLLRSRDPEEVIKQAQQLVDAGYKEIVLTGIHTGGYGEDMKDYNFAKLLSELDTRVEGVKRIRISSIEASQITD
EVIEVLDRSDKIVNHLHIPIQSGSNTVLKRMRRKYTMEFFADRLNKLKKALPGLAVTSDVIVGFPGETEEEFMETYNFIK
EHKFSELHVFPYSKRTGTPAARMEDQVDENVKNERVHRLIALSDQLAKEYASQYENEVLEIIPEEAFKETEEENMFVGYT
DNYMKVVFKGTEDMIGKIVKVKILKAGYPYNEGQFVRVVEDEITEHMRLSS
>Mature_450_residues
ATVAFHTLGCKVNHYETEAIWQLFKEAGYERRDFEQTADVYVINTCTVTNTGDKKSRQVIRRAIRQNPDGVICVTGCYAQ
TSPAEIMAIPGVDIVVGTQDREKMLGYIDQYREERQPINGVSNIMKARVYEELDVPAFTDRTRASLKIQEGCNNFCTFCI
IPWARGLLRSRDPEEVIKQAQQLVDAGYKEIVLTGIHTGGYGEDMKDYNFAKLLSELDTRVEGVKRIRISSIEASQITDE
VIEVLDRSDKIVNHLHIPIQSGSNTVLKRMRRKYTMEFFADRLNKLKKALPGLAVTSDVIVGFPGETEEEFMETYNFIKE
HKFSELHVFPYSKRTGTPAARMEDQVDENVKNERVHRLIALSDQLAKEYASQYENEVLEIIPEEAFKETEEENMFVGYTD
NYMKVVFKGTEDMIGKIVKVKILKAGYPYNEGQFVRVVEDEITEHMRLSS

Specific function: Unknown

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=455, Percent_Identity=29.8901098901099, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI28872784, Length=453, Percent_Identity=28.476821192053, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI28872782, Length=453, Percent_Identity=28.476821192053, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=33.9325842696629, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1787057, Length=390, Percent_Identity=32.3076923076923, Blast_Score=185, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17553146, Length=395, Percent_Identity=29.3670886075949, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71996771, Length=281, Percent_Identity=30.2491103202847, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356207, Length=444, Percent_Identity=28.6036036036036, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI19922432, Length=434, Percent_Identity=28.5714285714286, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YQEV_BACSU (P54462)

Other databases:

- EMBL:   D84432
- EMBL:   D83717
- EMBL:   AL009126
- PIR:   E69952
- RefSeq:   NP_390421.1
- ProteinModelPortal:   P54462
- SMR:   P54462
- EnsemblBacteria:   EBBACT00000000539
- GeneID:   937857
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25430
- NMPDR:   fig|224308.1.peg.2546
- GenoList:   BSU25430
- GeneTree:   EBGT00050000002619
- HOGENOM:   HBG457663
- OMA:   CTIPKAR
- PhylomeDB:   P54462
- ProtClustDB:   CLSK872997
- BioCyc:   BSUB:BSU25430-MONOMER
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006467
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01579
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51658; Mature: 51527

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVAFHTLGCKVNHYETEAIWQLFKEAGYERRDFEQTADVYVINTCTVTNTGDKKSRQV
CCEEEEEEECCEECCCHHHHHHHHHHHCCCCHHCHHCCCCEEEEEEEEECCCCCHHHHHH
IRRAIRQNPDGVICVTGCYAQTSPAEIMAIPGVDIVVGTQDREKMLGYIDQYREERQPIN
HHHHHHCCCCCEEEEEECCCCCCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHCCCCH
GVSNIMKARVYEELDVPAFTDRTRASLKIQEGCNNFCTFCIIPWARGLLRSRDPEEVIKQ
HHHHHHHHHHHHHCCCCCCCCCCHHHEEHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
AQQLVDAGYKEIVLTGIHTGGYGEDMKDYNFAKLLSELDTRVEGVKRIRISSIEASQITD
HHHHHHCCCHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
EVIEVLDRSDKIVNHLHIPIQSGSNTVLKRMRRKYTMEFFADRLNKLKKALPGLAVTSDV
HHHHHHCCCHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCE
IVGFPGETEEEFMETYNFIKEHKFSELHVFPYSKRTGTPAARMEDQVDENVKNERVHRLI
EEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALSDQLAKEYASQYENEVLEIIPEEAFKETEEENMFVGYTDNYMKVVFKGTEDMIGKIVK
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEECCCEEEEEECCCHHHHHHHHH
VKILKAGYPYNEGQFVRVVEDEITEHMRLSS
EEEEECCCCCCCCCEEEEHHHHHHHHHCCCC
>Mature Secondary Structure 
ATVAFHTLGCKVNHYETEAIWQLFKEAGYERRDFEQTADVYVINTCTVTNTGDKKSRQV
CEEEEEEECCEECCCHHHHHHHHHHHCCCCHHCHHCCCCEEEEEEEEECCCCCHHHHHH
IRRAIRQNPDGVICVTGCYAQTSPAEIMAIPGVDIVVGTQDREKMLGYIDQYREERQPIN
HHHHHHCCCCCEEEEEECCCCCCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHCCCCH
GVSNIMKARVYEELDVPAFTDRTRASLKIQEGCNNFCTFCIIPWARGLLRSRDPEEVIKQ
HHHHHHHHHHHHHCCCCCCCCCCHHHEEHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
AQQLVDAGYKEIVLTGIHTGGYGEDMKDYNFAKLLSELDTRVEGVKRIRISSIEASQITD
HHHHHHCCCHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
EVIEVLDRSDKIVNHLHIPIQSGSNTVLKRMRRKYTMEFFADRLNKLKKALPGLAVTSDV
HHHHHHCCCHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCE
IVGFPGETEEEFMETYNFIKEHKFSELHVFPYSKRTGTPAARMEDQVDENVKNERVHRLI
EEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALSDQLAKEYASQYENEVLEIIPEEAFKETEEENMFVGYTDNYMKVVFKGTEDMIGKIVK
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEECCCEEEEEECCCHHHHHHHHH
VKILKAGYPYNEGQFVRVVEDEITEHMRLSS
EEEEECCCCCCCCCEEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377