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The map label for this gene is era

Identifier: 16079583

GI number: 16079583

Start: 2610041

End: 2610946

Strand: Reverse

Name: era

Synonym: BSU25290

Alternate gene names: 16079583

Gene position: 2610946-2610041 (Counterclockwise)

Preceding gene: 16079584

Following gene: 255767578

Centisome position: 61.94

GC content: 43.16

Gene sequence:

>906_bases
ATGACGAACGAAAGCTTTAAATCAGGATTTGTATCCATTATTGGAAGACCAAACGTAGGAAAATCTACATTTTTGAACAG
AGTCATTGGACAAAAAATCGCGATCATGAGCGATAAGCCCCAAACGACGAGAAACAAGGTGCAAGGAGTACTGACGACAG
GCACATCACAAACCATTTTTATTGACACACCAGGGATTCATAAACCTAAGCATAAACTTGGGGACTTTATGATGAAAGTG
GCGCAAAATACATTAAAAGAAGTCGATTTGATCTTGTTTATGATCAATGCTGAGGAAGGCTACGGGAAGGGCGACGAATT
CATCATTGAGAAATTGCAGACGATGTCTACGCCTGTATTCTTAATCGTAAACAAAATTGATAAAATTCATCCCGACCAAT
TGCTTCTGCTAATTGACGAATACCGCAAACGTTATCCTTTTAAGGAAATTGTTCCGATTTCTGCTTTAGAAGGAAATAAC
ATCGAAACCTTGCTCGCGCAGATCGAAGCGTATTTGCCGGAAGGGCCGCAATTCTATCCGAGTGACCAGGTAACTGACCA
CCCTGAACGATTTATCATTTCTGAATTAATCAGGGAAAAGGTGCTGCATTTGACGAGAGAAGAAATCCCGCACAGCATTG
CGGTGGCCATCGAATCCATCAAAGGGCAGGACAACGGTTCTGTGCACGTAGCGGCAACGATTGTGGTCGAGCGGGATTCC
CAGAAAGGCATCGTGATCGGTAAAAAAGGAAGCCTTCTAAAAGAGGTTGGAAAAAGAGCGAGAGCGGATATTGAAGCACT
GTTGGGCTCCCGTGTCTACCTTGAATTGTGGGTGAAAGTGCAGAAAGACTGGCGTAATAAAATGTCCCAGCTTCGCGATT
TTGGCTTTAAAGAGGACGAATATTAA

Upstream 100 bases:

>100_bases
ACAAAAGACGTTATTGTCGTACTGACTAATTTACAAGGACAAATAAAAGAAATGACTGTGGAAGAATTATTGCCAGGCGC
ATTTTCATCGGAGGATTTAC

Downstream 100 bases:

>100_bases
TCTCTTCCCGTGTGTGTCATTATCTCACAGGAACAGGGATACATTAGCAAATTTTGTTTAACATGAAATGGAGGACATCT
GGTCAAGATAAAGGTAAGAA

Product: GTP-binding protein Era

Products: NA

Alternate protein names: Bex protein

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTNESFKSGFVSIIGRPNVGKSTFLNRVIGQKIAIMSDKPQTTRNKVQGVLTTGTSQTIFIDTPGIHKPKHKLGDFMMKV
AQNTLKEVDLILFMINAEEGYGKGDEFIIEKLQTMSTPVFLIVNKIDKIHPDQLLLLIDEYRKRYPFKEIVPISALEGNN
IETLLAQIEAYLPEGPQFYPSDQVTDHPERFIISELIREKVLHLTREEIPHSIAVAIESIKGQDNGSVHVAATIVVERDS
QKGIVIGKKGSLLKEVGKRARADIEALLGSRVYLELWVKVQKDWRNKMSQLRDFGFKEDEY

Sequences:

>Translated_301_residues
MTNESFKSGFVSIIGRPNVGKSTFLNRVIGQKIAIMSDKPQTTRNKVQGVLTTGTSQTIFIDTPGIHKPKHKLGDFMMKV
AQNTLKEVDLILFMINAEEGYGKGDEFIIEKLQTMSTPVFLIVNKIDKIHPDQLLLLIDEYRKRYPFKEIVPISALEGNN
IETLLAQIEAYLPEGPQFYPSDQVTDHPERFIISELIREKVLHLTREEIPHSIAVAIESIKGQDNGSVHVAATIVVERDS
QKGIVIGKKGSLLKEVGKRARADIEALLGSRVYLELWVKVQKDWRNKMSQLRDFGFKEDEY
>Mature_300_residues
TNESFKSGFVSIIGRPNVGKSTFLNRVIGQKIAIMSDKPQTTRNKVQGVLTTGTSQTIFIDTPGIHKPKHKLGDFMMKVA
QNTLKEVDLILFMINAEEGYGKGDEFIIEKLQTMSTPVFLIVNKIDKIHPDQLLLLIDEYRKRYPFKEIVPISALEGNNI
ETLLAQIEAYLPEGPQFYPSDQVTDHPERFIISELIREKVLHLTREEIPHSIAVAIESIKGQDNGSVHVAATIVVERDSQ
KGIVIGKKGSLLKEVGKRARADIEALLGSRVYLELWVKVQKDWRNKMSQLRDFGFKEDEY

Specific function: An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism. Binds both GDP and GTP. Complements

COG id: COG1159

COG function: function code R; GTPase

Gene ontology:

Cell location: Cytoplasm. Cell membrane; Peripheral membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KH type-2 domain

Homologues:

Organism=Homo sapiens, GI24307899, Length=323, Percent_Identity=23.8390092879257, Blast_Score=85, Evalue=6e-17,
Organism=Escherichia coli, GI1788919, Length=301, Percent_Identity=39.202657807309, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI87082120, Length=179, Percent_Identity=33.5195530726257, Blast_Score=99, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323665, Length=227, Percent_Identity=23.7885462555066, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646434, Length=313, Percent_Identity=26.8370607028754, Blast_Score=104, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ERA_BACSU (P42182)

Other databases:

- EMBL:   U18532
- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   X17430
- PIR:   B69593
- RefSeq:   NP_390407.1
- ProteinModelPortal:   P42182
- SMR:   P42182
- EnsemblBacteria:   EBBACT00000003334
- GeneID:   937871
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25290
- NMPDR:   fig|224308.1.peg.2532
- GenoList:   BSU25290
- GeneTree:   EBGT00070000032135
- HOGENOM:   HBG561652
- OMA:   AKDWQRD
- PhylomeDB:   P42182
- ProtClustDB:   PRK00089
- BioCyc:   BSUB:BSU25290-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00367
- InterPro:   IPR005662
- InterPro:   IPR006073
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004044
- InterPro:   IPR002917
- InterPro:   IPR005225
- Gene3D:   G3DSA:3.30.300.20
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00436
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF07650 KH_2; PF01926 MMR_HSR1; SSF54814 KH_prok

EC number: NA

Molecular weight: Translated: 34075; Mature: 33944

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50823 KH_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNESFKSGFVSIIGRPNVGKSTFLNRVIGQKIAIMSDKPQTTRNKVQGVLTTGTSQTIF
CCCCCHHCCHHEEECCCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHCEEEEECCCCEEE
IDTPGIHKPKHKLGDFMMKVAQNTLKEVDLILFMINAEEGYGKGDEFIIEKLQTMSTPVF
EECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCE
LIVNKIDKIHPDQLLLLIDEYRKRYPFKEIVPISALEGNNIETLLAQIEAYLPEGPQFYP
EEEHHHHCCCCHHHHHHHHHHHHHCCHHHCCEEECCCCCCHHHHHHHHHHHCCCCCCCCC
SDQVTDHPERFIISELIREKVLHLTREEIPHSIAVAIESIKGQDNGSVHVAATIVVERDS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEEEEEECC
QKGIVIGKKGSLLKEVGKRARADIEALLGSRVYLELWVKVQKDWRNKMSQLRDFGFKEDE
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
Y
C
>Mature Secondary Structure 
TNESFKSGFVSIIGRPNVGKSTFLNRVIGQKIAIMSDKPQTTRNKVQGVLTTGTSQTIF
CCCCHHCCHHEEECCCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHCEEEEECCCCEEE
IDTPGIHKPKHKLGDFMMKVAQNTLKEVDLILFMINAEEGYGKGDEFIIEKLQTMSTPVF
EECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCE
LIVNKIDKIHPDQLLLLIDEYRKRYPFKEIVPISALEGNNIETLLAQIEAYLPEGPQFYP
EEEHHHHCCCCHHHHHHHHHHHHHCCHHHCCEEECCCCCCHHHHHHHHHHHCCCCCCCCC
SDQVTDHPERFIISELIREKVLHLTREEIPHSIAVAIESIKGQDNGSVHVAATIVVERDS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEEEEEECC
QKGIVIGKKGSLLKEVGKRARADIEALLGSRVYLELWVKVQKDWRNKMSQLRDFGFKEDE
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 2526291