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The map label for this gene is pbpA

Identifier: 16079555

GI number: 16079555

Start: 2581771

End: 2583921

Strand: Reverse

Name: pbpA

Synonym: BSU25000

Alternate gene names: 16079555

Gene position: 2583921-2581771 (Counterclockwise)

Preceding gene: 255767566

Following gene: 16079554

Centisome position: 61.29

GC content: 39.89

Gene sequence:

>2151_bases
ATGAGGAGAAATAAACCAAAAAAGCAAAATCATAAAGAGAAAAAGAAGTCGCTTCCGATCCGGCTGAATATTTTATTTTT
AGCTGCCTTTGTTATATTTACCTGGATTATTGTGGAACTGGGCATCAAGCAGATCGTGCAAGGTGATGATTACAAAAACC
AAGCGAATAAGCAGGAACAAAGTGAAGTCAGCTCAGCGGTACCACGCGGGAAAATTTATGATCGAAACTTTAATGCCATT
GTGACAAATAAGGCTTTAAATGCAATCACATACACGAGATCTAAATCGACCACGCAAGAACAGCGCTTAAAGATCGCAAA
AAAGCTGTCTGACATGATCAAAGTCGATACAAAAAAAGTAACAGAACGGGATAAAAAAGATTATTGGATTTTGACTAGAC
CGAAAGAAGCGAAAAAGCTCATTAGCTCTAAAGAAAGACAGCAGGTTGAAGACAAAAAAATATCCGATGACGATTTATAC
CAGCTTCAGCTAAAACGGATTACTGATAAGCAGCTGAATGAATTGACGGATAAAGATATGCAGATCCTTGCTATCAAAAG
ACAGATGGATTCGGGCTATGCATTGACCCCTCAATATATCAAAAACGAAGATGTATCAGCGAAGGAAATGGCAGTGGTAA
GTGAACATTTAGACGAACTGCCAGGTGTTGATGTCACGTCCGATTGGGAACGTGAATATCCTTATAAAAACCTTCTTCGC
TCAGTGCTCGGAAGTGTTTCCAGCTCTAATGAAGGATTGCCGTCCAACTTGCTCGACCATTATCTGTCGCTTGGCTACAG
CCGAAATGACAGGGTCGGAAAAAGCTATCTGGAATACCAATATGAAAGCCTTCTGCAGGGCCAAAAAGCAAAAGTCGAAA
ATATCACAGATTCAAAAGGCAATGTCACGGGAACAAAAACGGTATCTGAAGGCAAAGCCGGGAAAGATCTTGTACTAACA
ATTGATATTGATTTGCAAAAATCAGTGGAAAAAATTATTGAGAAAAAACTTAAAGCTGCTAAAGCAAGACCTAGTACCGA
ATTGTTGGATCGTGCATTCGTTGTCATGATGGACCCTAGAAACGGCGAAGTGCTGACAATGGCAGGGAAGCAGATTAAAC
GAGAAAATGGAGCCTATAAGTTTGATGATTATGCATTAGGAGCCATGACTTCATCCTACGCTATGGGATCAGCTGTAAAA
GGTGCAACCGTTTTGACCGGTCTTCAAACTGGAGCGATTAATTTGAATACTGTATTTAAGGATGAACCATTGTATATCGG
CCAGGATAAAAGAGGGAAAAAATCTTGGCAAAACTTAGGGCCGGTTGGTATTCAAACTGCATTAGAGAAGTCATCAAACG
TTTTTATGTTTAAAACAGCAATTGCAGTTGGTAAAGGTGAATATAAACCTCACCAAGCATTGCCTTTGGATACTTCTGCA
TTTGATACCTTCAGAAATTATTTCAGTCAATTTGGATTAGGAGTAAAAACAGGAATTGATCTACCAAATGAAATGACGGG
ATATAAAGGTACAAGCAGATTATCTGGTTTCTTGCTTGATTTCGCCATTGGACAGTATGATACTTATACACCGCTGGAAT
TGGCTCAATATGTCTCCACCATAGCAAATGGTGGCTACCGCATGAAGCCTCAGCTTGTAAAGGAAGTCAGAGATTCAAAT
GCGAAAAAAGGGATCGGCGCAGTCGTTGATTCTGTTCAGCCTGAAGTATTGAATAAAGTCGATATGAAGAGTAGCTATAT
TGAAGAGGTCCAAGCCGGTTTTAGACGGGTTGCCACTAAAGGGACAGCTGCAGGTCAACTGGCTTCAGCATCGTATAAAC
CAGCTGCAAAAACAGGAACAGCCCAATCATTTTACGACGGACCGGATAAATCAAAAACAGGAACAGACACTTATAATACG
ACGCTTGTGGCTTATGCACCAGCTGACAATCCTGAGATTGCTATTTCTGTCGTAGTTCCTTGGACTTACATTGATTACAA
TCAAAGATACTCAATTACAAACGAAATCGGCAGGGAAGTAATGGATAAATATTTTGAGCTGAAATCGAAGCAGGACAAAG
AAGGTACTCAGCAAAAGAATAAAGACAAAATTGAAGAAAATGCAGAAAACACAACGTCTTCTGATAACTAA

Upstream 100 bases:

>100_bases
AAGGGGTTATCTGTAGAAAAAAGTCGAAAAAACCTTTAAAATGAAAACTGGCACTCTTACTTGAGTGTCTTTTCTTTCTG
TAAATAGAAAAGGTGATGTT

Downstream 100 bases:

>100_bases
AAAAAAACGCTCACATGATGTGGGCGTTTTTTTTATACAAAAAACGCACTGATTTACAAAACCTTAACATTCGGTTCAAA
CCCTTTTTACATAGAACCTT

Product: transpeptidase (penicillin-binding protein 2A)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MRRNKPKKQNHKEKKKSLPIRLNILFLAAFVIFTWIIVELGIKQIVQGDDYKNQANKQEQSEVSSAVPRGKIYDRNFNAI
VTNKALNAITYTRSKSTTQEQRLKIAKKLSDMIKVDTKKVTERDKKDYWILTRPKEAKKLISSKERQQVEDKKISDDDLY
QLQLKRITDKQLNELTDKDMQILAIKRQMDSGYALTPQYIKNEDVSAKEMAVVSEHLDELPGVDVTSDWEREYPYKNLLR
SVLGSVSSSNEGLPSNLLDHYLSLGYSRNDRVGKSYLEYQYESLLQGQKAKVENITDSKGNVTGTKTVSEGKAGKDLVLT
IDIDLQKSVEKIIEKKLKAAKARPSTELLDRAFVVMMDPRNGEVLTMAGKQIKRENGAYKFDDYALGAMTSSYAMGSAVK
GATVLTGLQTGAINLNTVFKDEPLYIGQDKRGKKSWQNLGPVGIQTALEKSSNVFMFKTAIAVGKGEYKPHQALPLDTSA
FDTFRNYFSQFGLGVKTGIDLPNEMTGYKGTSRLSGFLLDFAIGQYDTYTPLELAQYVSTIANGGYRMKPQLVKEVRDSN
AKKGIGAVVDSVQPEVLNKVDMKSSYIEEVQAGFRRVATKGTAAGQLASASYKPAAKTGTAQSFYDGPDKSKTGTDTYNT
TLVAYAPADNPEIAISVVVPWTYIDYNQRYSITNEIGREVMDKYFELKSKQDKEGTQQKNKDKIEENAENTTSSDN

Sequences:

>Translated_716_residues
MRRNKPKKQNHKEKKKSLPIRLNILFLAAFVIFTWIIVELGIKQIVQGDDYKNQANKQEQSEVSSAVPRGKIYDRNFNAI
VTNKALNAITYTRSKSTTQEQRLKIAKKLSDMIKVDTKKVTERDKKDYWILTRPKEAKKLISSKERQQVEDKKISDDDLY
QLQLKRITDKQLNELTDKDMQILAIKRQMDSGYALTPQYIKNEDVSAKEMAVVSEHLDELPGVDVTSDWEREYPYKNLLR
SVLGSVSSSNEGLPSNLLDHYLSLGYSRNDRVGKSYLEYQYESLLQGQKAKVENITDSKGNVTGTKTVSEGKAGKDLVLT
IDIDLQKSVEKIIEKKLKAAKARPSTELLDRAFVVMMDPRNGEVLTMAGKQIKRENGAYKFDDYALGAMTSSYAMGSAVK
GATVLTGLQTGAINLNTVFKDEPLYIGQDKRGKKSWQNLGPVGIQTALEKSSNVFMFKTAIAVGKGEYKPHQALPLDTSA
FDTFRNYFSQFGLGVKTGIDLPNEMTGYKGTSRLSGFLLDFAIGQYDTYTPLELAQYVSTIANGGYRMKPQLVKEVRDSN
AKKGIGAVVDSVQPEVLNKVDMKSSYIEEVQAGFRRVATKGTAAGQLASASYKPAAKTGTAQSFYDGPDKSKTGTDTYNT
TLVAYAPADNPEIAISVVVPWTYIDYNQRYSITNEIGREVMDKYFELKSKQDKEGTQQKNKDKIEENAENTTSSDN
>Mature_716_residues
MRRNKPKKQNHKEKKKSLPIRLNILFLAAFVIFTWIIVELGIKQIVQGDDYKNQANKQEQSEVSSAVPRGKIYDRNFNAI
VTNKALNAITYTRSKSTTQEQRLKIAKKLSDMIKVDTKKVTERDKKDYWILTRPKEAKKLISSKERQQVEDKKISDDDLY
QLQLKRITDKQLNELTDKDMQILAIKRQMDSGYALTPQYIKNEDVSAKEMAVVSEHLDELPGVDVTSDWEREYPYKNLLR
SVLGSVSSSNEGLPSNLLDHYLSLGYSRNDRVGKSYLEYQYESLLQGQKAKVENITDSKGNVTGTKTVSEGKAGKDLVLT
IDIDLQKSVEKIIEKKLKAAKARPSTELLDRAFVVMMDPRNGEVLTMAGKQIKRENGAYKFDDYALGAMTSSYAMGSAVK
GATVLTGLQTGAINLNTVFKDEPLYIGQDKRGKKSWQNLGPVGIQTALEKSSNVFMFKTAIAVGKGEYKPHQALPLDTSA
FDTFRNYFSQFGLGVKTGIDLPNEMTGYKGTSRLSGFLLDFAIGQYDTYTPLELAQYVSTIANGGYRMKPQLVKEVRDSN
AKKGIGAVVDSVQPEVLNKVDMKSSYIEEVQAGFRRVATKGTAAGQLASASYKPAAKTGTAQSFYDGPDKSKTGTDTYNT
TLVAYAPADNPEIAISVVVPWTYIDYNQRYSITNEIGREVMDKYFELKSKQDKEGTQQKNKDKIEENAENTTSSDN

Specific function: Cell Wall Formation; Pbp-2 Is Responsible For The Determination Of The Rod Shape Of The Cell. Its Synthesize Cross- Linked Peptidoglycan From The Lipid Intermediates. [C]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786854, Length=532, Percent_Identity=27.0676691729323, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786272, Length=411, Percent_Identity=27.7372262773723, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YQGF_BACSU (P54488)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   E69672
- RefSeq:   NP_390379.1
- ProteinModelPortal:   P54488
- EnsemblBacteria:   EBBACT00000000987
- GeneID:   938199
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25000
- NMPDR:   fig|224308.1.peg.2504
- GenoList:   BSU25000
- GeneTree:   EBGT00070000031810
- HOGENOM:   HBG314156
- OMA:   PVPRGKI
- ProtClustDB:   CLSK2460936
- BioCyc:   BSUB:BSU25000-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase; SSF56519 PBP_dimer; SSF56601 PBP_transp_fold

EC number: NA

Molecular weight: Translated: 80143; Mature: 80143

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: ACT_SITE 397-397

Signals:

None

Transmembrane regions:

HASH(0xc3070e0)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNKPKKQNHKEKKKSLPIRLNILFLAAFVIFTWIIVELGIKQIVQGDDYKNQANKQEQ
CCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHH
SEVSSAVPRGKIYDRNFNAIVTNKALNAITYTRSKSTTQEQRLKIAKKLSDMIKVDTKKV
HHHHHCCCCCCEECCCCCEEEECCHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
TERDKKDYWILTRPKEAKKLISSKERQQVEDKKISDDDLYQLQLKRITDKQLNELTDKDM
HHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
QILAIKRQMDSGYALTPQYIKNEDVSAKEMAVVSEHLDELPGVDVTSDWEREYPYKNLLR
HHHHHHHHHCCCCEECHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHH
SVLGSVSSSNEGLPSNLLDHYLSLGYSRNDRVGKSYLEYQYESLLQGQKAKVENITDSKG
HHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
NVTGTKTVSEGKAGKDLVLTIDIDLQKSVEKIIEKKLKAAKARPSTELLDRAFVVMMDPR
CCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCC
NGEVLTMAGKQIKRENGAYKFDDYALGAMTSSYAMGSAVKGATVLTGLQTGAINLNTVFK
CCCEEEECCHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCCHHHEEECCCCCEEEEEEEC
DEPLYIGQDKRGKKSWQNLGPVGIQTALEKSSNVFMFKTAIAVGKGEYKPHQALPLDTSA
CCCEEECCCCCCHHHHHHCCCHHHHHHHHCCCCEEEEEEEEHCCCCCCCCCCCCCCCHHH
FDTFRNYFSQFGLGVKTGIDLPNEMTGYKGTSRLSGFLLDFAIGQYDTYTPLELAQYVST
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IANGGYRMKPQLVKEVRDSNAKKGIGAVVDSVQPEVLNKVDMKSSYIEEVQAGFRRVATK
HHCCCEEECHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTAAGQLASASYKPAAKTGTAQSFYDGPDKSKTGTDTYNTTLVAYAPADNPEIAISVVVP
CCCCCHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEE
WTYIDYNQRYSITNEIGREVMDKYFELKSKQDKEGTQQKNKDKIEENAENTTSSDN
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCH
>Mature Secondary Structure
MRRNKPKKQNHKEKKKSLPIRLNILFLAAFVIFTWIIVELGIKQIVQGDDYKNQANKQEQ
CCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHH
SEVSSAVPRGKIYDRNFNAIVTNKALNAITYTRSKSTTQEQRLKIAKKLSDMIKVDTKKV
HHHHHCCCCCCEECCCCCEEEECCHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
TERDKKDYWILTRPKEAKKLISSKERQQVEDKKISDDDLYQLQLKRITDKQLNELTDKDM
HHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
QILAIKRQMDSGYALTPQYIKNEDVSAKEMAVVSEHLDELPGVDVTSDWEREYPYKNLLR
HHHHHHHHHCCCCEECHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHH
SVLGSVSSSNEGLPSNLLDHYLSLGYSRNDRVGKSYLEYQYESLLQGQKAKVENITDSKG
HHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
NVTGTKTVSEGKAGKDLVLTIDIDLQKSVEKIIEKKLKAAKARPSTELLDRAFVVMMDPR
CCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCC
NGEVLTMAGKQIKRENGAYKFDDYALGAMTSSYAMGSAVKGATVLTGLQTGAINLNTVFK
CCCEEEECCHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCCHHHEEECCCCCEEEEEEEC
DEPLYIGQDKRGKKSWQNLGPVGIQTALEKSSNVFMFKTAIAVGKGEYKPHQALPLDTSA
CCCEEECCCCCCHHHHHHCCCHHHHHHHHCCCCEEEEEEEEHCCCCCCCCCCCCCCCHHH
FDTFRNYFSQFGLGVKTGIDLPNEMTGYKGTSRLSGFLLDFAIGQYDTYTPLELAQYVST
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IANGGYRMKPQLVKEVRDSNAKKGIGAVVDSVQPEVLNKVDMKSSYIEEVQAGFRRVATK
HHCCCEEECHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTAAGQLASASYKPAAKTGTAQSFYDGPDKSKTGTDTYNTTLVAYAPADNPEIAISVVVP
CCCCCHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEE
WTYIDYNQRYSITNEIGREVMDKYFELKSKQDKEGTQQKNKDKIEENAENTTSSDN
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377