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The map label for this gene is yqhM

Identifier: 16079509

GI number: 16079509

Start: 2543968

End: 2544804

Strand: Reverse

Name: yqhM

Synonym: BSU24530

Alternate gene names: 16079509

Gene position: 2544804-2543968 (Counterclockwise)

Preceding gene: 16079511

Following gene: 255767549

Centisome position: 60.37

GC content: 44.09

Gene sequence:

>837_bases
ATGCAAAAAGAAACTTGGCGGTTTATAGACTCAGGCAATGCAAGTCCTGCATTCAATATGGCGCTTGATGAAGCGCTTTT
ATATTGGCACAGCGAAAAGAAAATCCCCCCGGTTATCCGTTTTTACGGCTGGAATCCGGCGACACTTTCTGTAGGGTATT
TTCAAAATATTAAAAAGGAAATCAATTTTGAAGCAGTACATAAATATAATCTCGGATTCGTCAGACGCCCGACCGGCGGG
AGAGGTGTGCTGCACGATCAGGAACTGACATACAGCGTAATTGTTTCGGAAGAGCACCCCGAGATGCCGGCGACTGTTAC
TGAGGCTTACCGGGTTATTTCAGAAGGCATACTTCAAGGCTTTAGAAATCTCGGTCTGGACGCTTACTTTGCCATCCCGA
GAACTGAAAAAGAAAAAGAAAGCTTAAAAAATCCGCGGTCATCTGTTTGTTTTGACGCGCCTTCGTGGTATGAACTAGTG
GTTGAGGGGCGCAAGGTGGCGGGAAGCGCGCAAACGAGACAAAAAGGTGTGATTCTTCAGCATGGCTCCATTCTTCTTGA
TTTAGATGAGGACAAGCTGTTCGATCTGTTCTTATATCCAAGTGAAAGAGTAAGAGAGCGGATGCAGCGCAATTTCAAAA
ATAAAGCGGTTGCCATTAATGAACTCATCGAAAAGCGGGTGACGATGGATGAGGCTCGTAAAGCCTTTAAAGAAGGATTT
GAAACAGGTCTTAACATTCATTTGGAGCCATATGAACTTTCTCAGGAAGAGCTTGATTTTGTTCATCACTTGGCTGAAAC
AAAGTACGCTTCAGATGAATGGAATTATAAGCGGTAA

Upstream 100 bases:

>100_bases
AGCACAGCGTGATTGTCATCTATAATGGAACTAAAATATTTTTCATTTTCCCGACAGTTTGATAAACTGATTCTATTAAA
ACGGTAAGGAAGGTCTTAAA

Downstream 100 bases:

>100_bases
ACAGGGCTTTTGACCCGCGGAGCATCCTTGTTTTTTCAAGTTCATTAGTGTAAACTTGTAATGGTTTATAAAGTTAAGGT
GCTTTTTGGGAGGGTTTCGA

Product: lipoate protein ligase

Products: lipoyl-AMP; pyrophosphate; N6-lipoyl-lysine [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MQKETWRFIDSGNASPAFNMALDEALLYWHSEKKIPPVIRFYGWNPATLSVGYFQNIKKEINFEAVHKYNLGFVRRPTGG
RGVLHDQELTYSVIVSEEHPEMPATVTEAYRVISEGILQGFRNLGLDAYFAIPRTEKEKESLKNPRSSVCFDAPSWYELV
VEGRKVAGSAQTRQKGVILQHGSILLDLDEDKLFDLFLYPSERVRERMQRNFKNKAVAINELIEKRVTMDEARKAFKEGF
ETGLNIHLEPYELSQEELDFVHHLAETKYASDEWNYKR

Sequences:

>Translated_278_residues
MQKETWRFIDSGNASPAFNMALDEALLYWHSEKKIPPVIRFYGWNPATLSVGYFQNIKKEINFEAVHKYNLGFVRRPTGG
RGVLHDQELTYSVIVSEEHPEMPATVTEAYRVISEGILQGFRNLGLDAYFAIPRTEKEKESLKNPRSSVCFDAPSWYELV
VEGRKVAGSAQTRQKGVILQHGSILLDLDEDKLFDLFLYPSERVRERMQRNFKNKAVAINELIEKRVTMDEARKAFKEGF
ETGLNIHLEPYELSQEELDFVHHLAETKYASDEWNYKR
>Mature_278_residues
MQKETWRFIDSGNASPAFNMALDEALLYWHSEKKIPPVIRFYGWNPATLSVGYFQNIKKEINFEAVHKYNLGFVRRPTGG
RGVLHDQELTYSVIVSEEHPEMPATVTEAYRVISEGILQGFRNLGLDAYFAIPRTEKEKESLKNPRSSVCFDAPSWYELV
VEGRKVAGSAQTRQKGVILQHGSILLDLDEDKLFDLFLYPSERVRERMQRNFKNKAVAINELIEKRVTMDEARKAFKEGF
ETGLNIHLEPYELSQEELDFVHHLAETKYASDEWNYKR

Specific function: Catalyzes Both The ATP-Dependent Activation Of Exogenously Supplied Lipoate To Lipoyl-Amp And The Transfer Of The Activated Lipoyl On The Lipoate-Dependent Enzymes. Creates An Amide Linkage That Joins The Free Carboxyl Group Of Lipoic Acid To The Epsilon-

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YQHM_BACSU (P54511)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   D69959
- RefSeq:   NP_390333.1
- ProteinModelPortal:   P54511
- SMR:   P54511
- EnsemblBacteria:   EBBACT00000003881
- GeneID:   938551
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24530
- NMPDR:   fig|224308.1.peg.2457
- GenoList:   BSU24530
- GeneTree:   EBGT00050000002150
- HOGENOM:   HBG650514
- OMA:   ETWRFID
- PhylomeDB:   P54511
- ProtClustDB:   CLSK873436
- BioCyc:   BSUB:BSU24530-MONOMER
- InterPro:   IPR004143

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: 6.3.2.- [C]

Molecular weight: Translated: 32241; Mature: 32241

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKETWRFIDSGNASPAFNMALDEALLYWHSEKKIPPVIRFYGWNPATLSVGYFQNIKKE
CCCCCEEHEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEHHHHHHHHHH
INFEAVHKYNLGFVRRPTGGRGVLHDQELTYSVIVSEEHPEMPATVTEAYRVISEGILQG
CCHHHEEEECCCEEECCCCCCCCEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
FRNLGLDAYFAIPRTEKEKESLKNPRSSVCFDAPSWYELVVEGRKVAGSAQTRQKGVILQ
HHHCCCEEEEECCCCCHHHHHHCCCCCCEEECCCCHHHHHHHCHHHCCCCHHHHCCEEEE
HGSILLDLDEDKLFDLFLYPSERVRERMQRNFKNKAVAINELIEKRVTMDEARKAFKEGF
CCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ETGLNIHLEPYELSQEELDFVHHLAETKYASDEWNYKR
HCCCEEEECCHHCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQKETWRFIDSGNASPAFNMALDEALLYWHSEKKIPPVIRFYGWNPATLSVGYFQNIKKE
CCCCCEEHEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEHHHHHHHHHH
INFEAVHKYNLGFVRRPTGGRGVLHDQELTYSVIVSEEHPEMPATVTEAYRVISEGILQG
CCHHHEEEECCCEEECCCCCCCCEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
FRNLGLDAYFAIPRTEKEKESLKNPRSSVCFDAPSWYELVVEGRKVAGSAQTRQKGVILQ
HHHCCCEEEEECCCCCHHHHHHCCCCCCEEECCCCHHHHHHHCHHHCCCCHHHHCCEEEE
HGSILLDLDEDKLFDLFLYPSERVRERMQRNFKNKAVAINELIEKRVTMDEARKAFKEGF
CCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ETGLNIHLEPYELSQEELDFVHHLAETKYASDEWNYKR
HCCCEEEECCHHCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipoic acid; ATP [C]

Specific reaction: Catalyzes first the reaction of lipoic acid and ATP to form lipoyl-AMP and pyrophosphate, then the formation N6-lipoyl-lysine from a specific lysine residue in lipoate-dependent enzymes [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Other Carbon-Nitrogen Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377