Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is proI

Identifier: 16079437

GI number: 16079437

Start: 2473151

End: 2473987

Strand: Direct

Name: proI

Synonym: BSU23800

Alternate gene names: 16079437

Gene position: 2473151-2473987 (Clockwise)

Preceding gene: 16079431

Following gene: 16079439

Centisome position: 58.67

GC content: 46.59

Gene sequence:

>837_bases
ATGAAAAAGATAGGATTTGTCGGCGCCGGGTCAATGGCAGAAGCAATGATCAACGGCATTTTGCAAAGCGGCATCACAAA
ACCAGAACATATCTATATTACAAATCGCTCAAATGACGAGCGTCTGATTGAATTAAAGGAAACGTACAGCGTGCGTCCAT
GCAGAGATAAAAATGAGTTTTTCACTCACACCGATATCATTATCCTCGCTTTCAAACCGAAAGACGCGGCGGAAAGCATC
GACAGCATCCGGCCGTATATCAAGGACCAGCTTGTCATTTCAGTACTTGCAGGCCTTACCATCGAAACCATTCAGCATTA
TTTTGGCAGAAAGCTCGCGGTCATTCGCGTCATGCCTAATACATCAGCGGCAATCAGAAAATCAGCAACCGGATTTTCCG
TAAGCACGGAAGCAAGCAAGAATGATATTATTGCCGCAAAAGCTCTATTGGAAACAATCGGTGATGCAACACTGGTTGAG
GAGCGGCATCTTGACGCTGTCACTGCAATCGCAGGCAGCGGCCCGGCCTATGTGTATCGTTATATTGAGGCGATGGAAAA
AGCAGCACAAAAAGTAGGATTAGATAAAGAAACGGCAAAAGCACTCATTTTGCAGACAATGGCAGGGGCAACAGATATGC
TCCTCCAAAGCGGCAAACAGCCGGAAAAGCTGCGTAAAGAAATCACCAGTCCTGGGGGCACTACAGAGGCAGGTCTTCGC
GCTTTACAGGACTCCCGCTTTGAAGAAGCAATTATTCACTGTATTGAAGAAACAGCTAAACGGAGCGCAGAAATCAAAGA
ACAGTTTGCCGGTGCCGCCTTAGAGAGACATTCTTAG

Upstream 100 bases:

>100_bases
AGCTGAGCACAATTTGTGCTAATGAGGGTGGTACCGCGAACCTTTTCGTCCTTTACGTGATGAAAAGGTTTTTTGTTGTT
CTAATAGAAGGAGGATATCA

Downstream 100 bases:

>100_bases
ATGTAAGGACAAAAACAGAAGGCACAGTGCCTTCTGTTTTTTATTTTTCCCGCAGCAGCTGGCCGGCAGATTCTGATTGC
TTTAATAGCGCGGAGATCGT

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase 2; P5CR 2

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKKIGFVGAGSMAEAMINGILQSGITKPEHIYITNRSNDERLIELKETYSVRPCRDKNEFFTHTDIIILAFKPKDAAESI
DSIRPYIKDQLVISVLAGLTIETIQHYFGRKLAVIRVMPNTSAAIRKSATGFSVSTEASKNDIIAAKALLETIGDATLVE
ERHLDAVTAIAGSGPAYVYRYIEAMEKAAQKVGLDKETAKALILQTMAGATDMLLQSGKQPEKLRKEITSPGGTTEAGLR
ALQDSRFEEAIIHCIEETAKRSAEIKEQFAGAALERHS

Sequences:

>Translated_278_residues
MKKIGFVGAGSMAEAMINGILQSGITKPEHIYITNRSNDERLIELKETYSVRPCRDKNEFFTHTDIIILAFKPKDAAESI
DSIRPYIKDQLVISVLAGLTIETIQHYFGRKLAVIRVMPNTSAAIRKSATGFSVSTEASKNDIIAAKALLETIGDATLVE
ERHLDAVTAIAGSGPAYVYRYIEAMEKAAQKVGLDKETAKALILQTMAGATDMLLQSGKQPEKLRKEITSPGGTTEAGLR
ALQDSRFEEAIIHCIEETAKRSAEIKEQFAGAALERHS
>Mature_278_residues
MKKIGFVGAGSMAEAMINGILQSGITKPEHIYITNRSNDERLIELKETYSVRPCRDKNEFFTHTDIIILAFKPKDAAESI
DSIRPYIKDQLVISVLAGLTIETIQHYFGRKLAVIRVMPNTSAAIRKSATGFSVSTEASKNDIIAAKALLETIGDATLVE
ERHLDAVTAIAGSGPAYVYRYIEAMEKAAQKVGLDKETAKALILQTMAGATDMLLQSGKQPEKLRKEITSPGGTTEAGLR
ALQDSRFEEAIIHCIEETAKRSAEIKEQFAGAALERHS

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family

Homologues:

Organism=Homo sapiens, GI24797095, Length=282, Percent_Identity=32.2695035460993, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI24797097, Length=282, Percent_Identity=32.2695035460993, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI21361454, Length=290, Percent_Identity=30.6896551724138, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI198041662, Length=268, Percent_Identity=27.9850746268657, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI1786585, Length=266, Percent_Identity=31.203007518797, Blast_Score=147, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17569021, Length=261, Percent_Identity=32.5670498084291, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17540664, Length=297, Percent_Identity=26.9360269360269, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6320861, Length=283, Percent_Identity=26.8551236749117, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648116, Length=277, Percent_Identity=33.9350180505415, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21358587, Length=263, Percent_Identity=33.0798479087452, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24647700, Length=169, Percent_Identity=37.2781065088757, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): P5CR2_BACSU (P54552)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   G69964
- RefSeq:   NP_390261.1
- ProteinModelPortal:   P54552
- SMR:   P54552
- EnsemblBacteria:   EBBACT00000000920
- GeneID:   938697
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23800
- NMPDR:   fig|224308.1.peg.2385
- GenoList:   BSU23800
- GeneTree:   EBGT00050000002535
- HOGENOM:   HBG726602
- OMA:   AMKPKDV
- PhylomeDB:   P54552
- ProtClustDB:   CLSK887537
- BioCyc:   BSUB:BSU23800-MONOMER
- BRENDA:   1.5.1.2
- GO:   GO:0005737
- GO:   GO:0005488
- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11645
- PIRSF:   PIRSF000193
- TIGRFAMs:   TIGR00112

Pfam domain/function: PF03807 F420_oxidored; SSF48179 6DGDH_C_like

EC number: =1.5.1.2

Molecular weight: Translated: 30396; Mature: 30396

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00521 P5CR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MKKIGFVGAGSMAEAMINGILQSGITKPEHIYITNRSNDERLIELKETYSVRPCRDKNEF
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHEEEHHHCCCCCCCCCCCE
FTHTDIIILAFKPKDAAESIDSIRPYIKDQLVISVLAGLTIETIQHYFGRKLAVIRVMPN
EEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
TSAAIRKSATGFSVSTEASKNDIIAAKALLETIGDATLVEERHLDAVTAIAGSGPAYVYR
CHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHH
YIEAMEKAAQKVGLDKETAKALILQTMAGATDMLLQSGKQPEKLRKEITSPGGTTEAGLR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHH
ALQDSRFEEAIIHCIEETAKRSAEIKEQFAGAALERHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKIGFVGAGSMAEAMINGILQSGITKPEHIYITNRSNDERLIELKETYSVRPCRDKNEF
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHEEEHHHCCCCCCCCCCCE
FTHTDIIILAFKPKDAAESIDSIRPYIKDQLVISVLAGLTIETIQHYFGRKLAVIRVMPN
EEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
TSAAIRKSATGFSVSTEASKNDIIAAKALLETIGDATLVEERHLDAVTAIAGSGPAYVYR
CHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHH
YIEAMEKAAQKVGLDKETAKALILQTMAGATDMLLQSGKQPEKLRKEITSPGGTTEAGLR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHH
ALQDSRFEEAIIHCIEETAKRSAEIKEQFAGAALERHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 11418582