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The map label for this gene is sigF

Identifier: 16079402

GI number: 16079402

Start: 2443429

End: 2444196

Strand: Reverse

Name: sigF

Synonym: BSU23450

Alternate gene names: 16079402

Gene position: 2444196-2443429 (Counterclockwise)

Preceding gene: 16079403

Following gene: 255767507

Centisome position: 57.98

GC content: 44.79

Gene sequence:

>768_bases
ATGGATGTGGAGGTTAAGAAAAACGGCAAAAACGCTCAGCTGAAGGATCATGAAGTAAAGGAATTAATCAAACAAAGCCA
AAATGGCGACCAGCAGGCAAGAGACCTCCTCATAGAAAAAAACATGCGTCTTGTTTGGTCTGTCGTACAGCGGTTTTTAA
ACAGAGGATATGAGCCTGACGATCTCTTCCAGATCGGCTGCATCGGGCTGTTAAAATCTGTTGACAAATTTGATTTAACC
TATGATGTGCGTTTTTCAACGTATGCAGTGCCGATGATTATCGGAGAAATCCAACGATTTATCCGTGATGACGGAACCGT
AAAGGTATCACGGTCATTAAAAGAGCTTGGAAACAAAATCCGGCGCGCGAAGGATGAGCTTTCGAAAACACTGGGCAGAG
TGCCGACGGTGCAGGAGATCGCTGACCATTTGGAGATTGAAGCTGAGGATGTTGTACTGGCCCAAGAGGCGGTAAGGGCT
CCATCTTCGATTCACGAAACCGTTTATGAAAATGACGGAGATCCGATTACCCTGCTTGATCAAATCGCTGACAACTCAGA
AGAAAAATGGTTTGACAAAATTGCGCTGAAAGAAGCGATCAGCGATTTGGAGGAAAGGGAAAAACTAATCGTCTATCTCA
GATATTATAAAGACCAGACACAGTCCGAGGTGGCTGAGCGGCTCGGGATCTCTCAGGTGCAGGTTTCCAGGCTTGAAAAG
AAAATATTAAAACAGATCAAGGTTCAAATGGATCATACGGATGGCTAG

Upstream 100 bases:

>100_bases
ATGATGTCAGTATCGATTCATCGCCTGAAATGGGAACAACGATTCGCTTAACAAAGCACTTATCAAAAAGCAAAGCGCTT
TGTAATTAAGGAGATTTGTT

Downstream 100 bases:

>100_bases
TCTGCAGTGCAGGCTAGCTTTTTTGTGCAAAAGCGTGGTAATTTATGGTCTTTTCGAGCGGATGAATGAGAACAAAATCG
AACCACATACTACATATATA

Product: sporulation sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MDVEVKKNGKNAQLKDHEVKELIKQSQNGDQQARDLLIEKNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLT
YDVRFSTYAVPMIIGEIQRFIRDDGTVKVSRSLKELGNKIRRAKDELSKTLGRVPTVQEIADHLEIEAEDVVLAQEAVRA
PSSIHETVYENDGDPITLLDQIADNSEEKWFDKIALKEAISDLEEREKLIVYLRYYKDQTQSEVAERLGISQVQVSRLEK
KILKQIKVQMDHTDG

Sequences:

>Translated_255_residues
MDVEVKKNGKNAQLKDHEVKELIKQSQNGDQQARDLLIEKNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLT
YDVRFSTYAVPMIIGEIQRFIRDDGTVKVSRSLKELGNKIRRAKDELSKTLGRVPTVQEIADHLEIEAEDVVLAQEAVRA
PSSIHETVYENDGDPITLLDQIADNSEEKWFDKIALKEAISDLEEREKLIVYLRYYKDQTQSEVAERLGISQVQVSRLEK
KILKQIKVQMDHTDG
>Mature_255_residues
MDVEVKKNGKNAQLKDHEVKELIKQSQNGDQQARDLLIEKNMRLVWSVVQRFLNRGYEPDDLFQIGCIGLLKSVDKFDLT
YDVRFSTYAVPMIIGEIQRFIRDDGTVKVSRSLKELGNKIRRAKDELSKTLGRVPTVQEIADHLEIEAEDVVLAQEAVRA
PSSIHETVYENDGDPITLLDQIADNSEEKWFDKIALKEAISDLEEREKLIVYLRYYKDQTQSEVAERLGISQVQVSRLEK
KILKQIKVQMDHTDG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. Interaction with spoIIAB inhibits sig

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

Organism=Escherichia coli, GI1789448, Length=245, Percent_Identity=28.9795918367347, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1789098, Length=253, Percent_Identity=28.4584980237154, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1788231, Length=199, Percent_Identity=29.6482412060301, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=26.3374485596708, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): RPSF_BACSU (P07860)

Other databases:

- EMBL:   M15744
- EMBL:   M17643
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   A28567
- RefSeq:   NP_390226.1
- ProteinModelPortal:   P07860
- SMR:   P07860
- EnsemblBacteria:   EBBACT00000004079
- GeneID:   938729
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23450
- NMPDR:   fig|224308.1.peg.2350
- GenoList:   BSU23450
- GeneTree:   EBGT00070000031820
- HOGENOM:   HBG745096
- OMA:   RDDGLIK
- ProtClustDB:   PRK05572
- BioCyc:   BSUB:BSU23450-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02980
- TIGRFAMs:   TIGR02937
- TIGRFAMs:   TIGR02885

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 29373; Mature: 29373

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVEVKKNGKNAQLKDHEVKELIKQSQNGDQQARDLLIEKNMRLVWSVVQRFLNRGYEPD
CCCEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCH
DLFQIGCIGLLKSVDKFDLTYDVRFSTYAVPMIIGEIQRFIRDDGTVKVSRSLKELGNKI
HHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RRAKDELSKTLGRVPTVQEIADHLEIEAEDVVLAQEAVRAPSSIHETVYENDGDPITLLD
HHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHH
QIADNSEEKWFDKIALKEAISDLEEREKLIVYLRYYKDQTQSEVAERLGISQVQVSRLEK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHH
KILKQIKVQMDHTDG
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MDVEVKKNGKNAQLKDHEVKELIKQSQNGDQQARDLLIEKNMRLVWSVVQRFLNRGYEPD
CCCEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCH
DLFQIGCIGLLKSVDKFDLTYDVRFSTYAVPMIIGEIQRFIRDDGTVKVSRSLKELGNKI
HHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RRAKDELSKTLGRVPTVQEIADHLEIEAEDVVLAQEAVRAPSSIHETVYENDGDPITLLD
HHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHH
QIADNSEEKWFDKIALKEAISDLEEREKLIVYLRYYKDQTQSEVAERLGISQVQVSRLEK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHH
KILKQIKVQMDHTDG
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3116160; 6088674; 8969508; 9384377; 8358793