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The map label for this gene is ribE

Identifier: 16079384

GI number: 16079384

Start: 2429600

End: 2430247

Strand: Reverse

Name: ribE

Synonym: BSU23270

Alternate gene names: 16079384

Gene position: 2430247-2429600 (Counterclockwise)

Preceding gene: 16079385

Following gene: 16079383

Centisome position: 57.65

GC content: 40.12

Gene sequence:

>648_bases
ATGTTTACAGGAATTATCGAAGAAACAGGCACAATCGAATCCATGAAAAAAGCAGGGCATGCAATGGCCTTAACTATTAA
ATGCTCAAAGATTTTAGAGGATGTTCATCTTGGCGACAGCATTGCAGTGAACGGCATTTGTCTGACTGTCACTGATTTTA
CAAAAAATCAATTCACAGTGGATGTTATGCCTGAAACAGTCAAAGCTACGTCACTGAATGATTTAACAAAAGGAAGCAAA
GTAAATCTGGAAAGAGCGATGGCGGCAAACGGCCGTTTCGGAGGCCATTTCGTCTCAGGCCATGTCGACGGAACTGCGGA
AATCACACGAATTGAAGAGAAAAGCAACGCAGTTTACTATGATTTAAAAATGGACCCGTCATTAACAAAAACATTGGTTT
TAAAGGGATCAATTACTGTGGATGGCGTGAGCTTAACCATATTCGGCCTGACAGAAGACACAGTGACGATCTCCTTAATA
CCGCATACGATCAGCGAAACGATCTTTTCAGAAAAAACGATCGGCTCTAAAGTGAATATCGAATGCGATATGATCGGAAA
ATATATGTATCGATTTTTGCATAAAGCCAATGAAAATAAGACCCAACAAACCATTACAAAAGCCTTCTTAAGCGAAAACG
GCTTTTAG

Upstream 100 bases:

>100_bases
TCAATGAAAGATGTCCCCTTATTACAATTCACTGATATAACCCAAATCGGCCGTGATATCAAACTGACGGCAAAACCGAC
AAAGGAATAGGATGGTGACC

Downstream 100 bases:

>100_bases
AGAGGAAGATTTGCATGTTTCATCCGATAGAAGAAGCACTGGACGCTTTAAAAAAAGGCGAAGTCATCATCGTTGTAGAT
GATGAAGACAGAGAAAATGA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MFTGIIEETGTIESMKKAGHAMALTIKCSKILEDVHLGDSIAVNGICLTVTDFTKNQFTVDVMPETVKATSLNDLTKGSK
VNLERAMAANGRFGGHFVSGHVDGTAEITRIEEKSNAVYYDLKMDPSLTKTLVLKGSITVDGVSLTIFGLTEDTVTISLI
PHTISETIFSEKTIGSKVNIECDMIGKYMYRFLHKANENKTQQTITKAFLSENGF

Sequences:

>Translated_215_residues
MFTGIIEETGTIESMKKAGHAMALTIKCSKILEDVHLGDSIAVNGICLTVTDFTKNQFTVDVMPETVKATSLNDLTKGSK
VNLERAMAANGRFGGHFVSGHVDGTAEITRIEEKSNAVYYDLKMDPSLTKTLVLKGSITVDGVSLTIFGLTEDTVTISLI
PHTISETIFSEKTIGSKVNIECDMIGKYMYRFLHKANENKTQQTITKAFLSENGF
>Mature_215_residues
MFTGIIEETGTIESMKKAGHAMALTIKCSKILEDVHLGDSIAVNGICLTVTDFTKNQFTVDVMPETVKATSLNDLTKGSK
VNLERAMAANGRFGGHFVSGHVDGTAEITRIEEKSNAVYYDLKMDPSLTKTLVLKGSITVDGVSLTIFGLTEDTVTISLI
PHTISETIFSEKTIGSKVNIECDMIGKYMYRFLHKANENKTQQTITKAFLSENGF

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats

Homologues:

Organism=Escherichia coli, GI1787952, Length=214, Percent_Identity=34.5794392523364, Blast_Score=124, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6319733, Length=212, Percent_Identity=40.0943396226415, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISA_BACSU (P16440)

Other databases:

- EMBL:   L09228
- EMBL:   X51510
- EMBL:   AL009126
- PIR:   S45544
- RefSeq:   NP_390208.1
- ProteinModelPortal:   P16440
- SMR:   P16440
- EnsemblBacteria:   EBBACT00000002822
- GeneID:   938947
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23270
- NMPDR:   fig|224308.1.peg.2331
- GenoList:   BSU23270
- GeneTree:   EBGT00050000001264
- HOGENOM:   HBG289809
- OMA:   PVNLEVD
- PhylomeDB:   P16440
- ProtClustDB:   PRK09289
- BioCyc:   BSUB:BSU23270-MONOMER
- BioCyc:   MetaCyc:MONOMER-14608
- BRENDA:   2.5.1.9
- InterPro:   IPR001783
- InterPro:   IPR017938
- PANTHER:   PTHR21098
- PIRSF:   PIRSF000498
- TIGRFAMs:   TIGR00187

Pfam domain/function: PF00677 Lum_binding; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =2.5.1.9

Molecular weight: Translated: 23481; Mature: 23481

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS51177 LUMAZINE_BIND; PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIIEETGTIESMKKAGHAMALTIKCSKILEDVHLGDSIAVNGICLTVTDFTKNQFTV
CCCCCCCCCCCHHHHHHCCCEEEEEEEHHHHHHHHCCCCCEEECCEEEEEEECCCCEEEE
DVMPETVKATSLNDLTKGSKVNLERAMAANGRFGGHFVSGHVDGTAEITRIEEKSNAVYY
EECCCCEEECCCCHHCCCCCCCHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCEEEE
DLKMDPSLTKTLVLKGSITVDGVSLTIFGLTEDTVTISLIPHTISETIFSEKTIGSKVNI
EEEECCCCEEEEEEECCEEECCEEEEEEEECCCEEEEEEECCHHHHHHHHHHCCCCEEEE
ECDMIGKYMYRFLHKANENKTQQTITKAFLSENGF
EHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFTGIIEETGTIESMKKAGHAMALTIKCSKILEDVHLGDSIAVNGICLTVTDFTKNQFTV
CCCCCCCCCCCHHHHHHCCCEEEEEEEHHHHHHHHCCCCCEEECCEEEEEEECCCCEEEE
DVMPETVKATSLNDLTKGSKVNLERAMAANGRFGGHFVSGHVDGTAEITRIEEKSNAVYY
EECCCCEEECCCCHHCCCCCCCHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCEEEE
DLKMDPSLTKTLVLKGSITVDGVSLTIFGLTEDTVTISLIPHTISETIFSEKTIGSKVNI
EEEECCCCEEEEEEECCEEECCEEEEEEEECCCEEEEEEECCHHHHHHHHHHCCCCEEEE
ECDMIGKYMYRFLHKANENKTQQTITKAFLSENGF
EHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2106516; 7934829; 9384377; 7473709