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The map label for this gene is dacB

Identifier: 16079376

GI number: 16079376

Start: 2423383

End: 2424531

Strand: Reverse

Name: dacB

Synonym: BSU23190

Alternate gene names: 16079376

Gene position: 2424531-2423383 (Counterclockwise)

Preceding gene: 16079377

Following gene: 16079375

Centisome position: 57.51

GC content: 41.25

Gene sequence:

>1149_bases
ATGCGCATTTTCAAAAAAGCAGTATTCGTGATCATGATTTCTTTTCTTATTGCAACCGTAAATGTGAATACAGCACATGC
TGCTATAGATGTCAGTGCAAAAAGCGCGATCATTATTGACGGTGCGTCAGGCAGAGTGTTATATGCCAAGGATGAGCATC
AAAAAAGGCGGATCGCGAGCATCACAAAAATTATGACCGCCGTCCTCGCCATCGAATCCGGCAAAATGGATCAAACAGTG
ACGGTTTCGGCAAATGCTGTCAGAACAGAAGGCTCCGCCATTTATTTAACAGAAGGTCAAAAAGTGAAGCTGAAAGATCT
TGTGTATGGCCTGATGCTAAGATCAGGAAACGATGCCGCTGTCGCGATTGCTGAGCATGTCGGTGGGAGCCTCGATGGGT
TTGTTTATATGATGAATCAAAAAGCCGAGCAGCTTGGTATGAAAAACACCCGTTTTCAAAACCCGCACGGATTGGACGAC
CATGAAAATCATTATTCAACAGCCTATGACATGGCCATTTTAACTAAGTATGCTATGAAGCTGAAAGACTATCAAAAGAT
TTCAGGCACAAAAATATACAAAGCGGAAACGATGGAAAGCGTATGGAAAAACAAAAACAAACTGCTGACCATGCTTTATC
CGTACAGCACAGGAGGAAAAACAGGCTATACAAAACTTGCAAAACGGACACTGGTCTCTACGGCTTCAAAGGACGGCATT
GACCTGATAGCCGTCACCATTAATGACCCTAATGATTGGGATGACCATATGAAGATGTTTAACTATGTGTTTGAGCATTA
TCAAACCTATTTAATTGCAAAAAAAGGGGATATTCCAAAGCTGAAAGGCACATTTTATGAATCGAAGGCTTTTATCAAAC
GTGATATCACCTATTTGCTGACCGAAGAGGAAAAAGAAAATGTAAAAATTAATACCACACTGCTCAAGCCGAAAAAAGCA
TGGGAAAAAGATGCCTCGAAAATCCCTGACATCGTAGGGCACATGGAGATCATGTTCAACGATGCAACCATCGCAAAAGT
GCCGATCTATTATGAAAATGAGCGGCATCAAAAACCGAAGAAACAATTTTTTGAAACGTTTAAATCAATCTTTCTGAATG
CGGCAGGCGGAGCAAAATGGTCAATATAA

Upstream 100 bases:

>100_bases
GAGAGTTTTTTTATGTTTGCTTCCAGAAATATAATTGTTATTCATAACTGATGGACATGCGCATAAACTTGTACAAACCA
CCACAAGGACGTGAGCAAAC

Downstream 100 bases:

>100_bases
TTTGGGTCAGTTTAACCGTGATCGGACTCGTTTTTGCGATGTGCAACGGAACTCTGCAAGATGTAAATGAAGCCGTGTTT
AAGGGTGCGAAAGAAGCGAT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5*

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MRIFKKAVFVIMISFLIATVNVNTAHAAIDVSAKSAIIIDGASGRVLYAKDEHQKRRIASITKIMTAVLAIESGKMDQTV
TVSANAVRTEGSAIYLTEGQKVKLKDLVYGLMLRSGNDAAVAIAEHVGGSLDGFVYMMNQKAEQLGMKNTRFQNPHGLDD
HENHYSTAYDMAILTKYAMKLKDYQKISGTKIYKAETMESVWKNKNKLLTMLYPYSTGGKTGYTKLAKRTLVSTASKDGI
DLIAVTINDPNDWDDHMKMFNYVFEHYQTYLIAKKGDIPKLKGTFYESKAFIKRDITYLLTEEEKENVKINTTLLKPKKA
WEKDASKIPDIVGHMEIMFNDATIAKVPIYYENERHQKPKKQFFETFKSIFLNAAGGAKWSI

Sequences:

>Translated_382_residues
MRIFKKAVFVIMISFLIATVNVNTAHAAIDVSAKSAIIIDGASGRVLYAKDEHQKRRIASITKIMTAVLAIESGKMDQTV
TVSANAVRTEGSAIYLTEGQKVKLKDLVYGLMLRSGNDAAVAIAEHVGGSLDGFVYMMNQKAEQLGMKNTRFQNPHGLDD
HENHYSTAYDMAILTKYAMKLKDYQKISGTKIYKAETMESVWKNKNKLLTMLYPYSTGGKTGYTKLAKRTLVSTASKDGI
DLIAVTINDPNDWDDHMKMFNYVFEHYQTYLIAKKGDIPKLKGTFYESKAFIKRDITYLLTEEEKENVKINTTLLKPKKA
WEKDASKIPDIVGHMEIMFNDATIAKVPIYYENERHQKPKKQFFETFKSIFLNAAGGAKWSI
>Mature_382_residues
MRIFKKAVFVIMISFLIATVNVNTAHAAIDVSAKSAIIIDGASGRVLYAKDEHQKRRIASITKIMTAVLAIESGKMDQTV
TVSANAVRTEGSAIYLTEGQKVKLKDLVYGLMLRSGNDAAVAIAEHVGGSLDGFVYMMNQKAEQLGMKNTRFQNPHGLDD
HENHYSTAYDMAILTKYAMKLKDYQKISGTKIYKAETMESVWKNKNKLLTMLYPYSTGGKTGYTKLAKRTLVSTASKDGI
DLIAVTINDPNDWDDHMKMFNYVFEHYQTYLIAKKGDIPKLKGTFYESKAFIKRDITYLLTEEEKENVKINTTLLKPKKA
WEKDASKIPDIVGHMEIMFNDATIAKVPIYYENERHQKPKKQFFETFKSIFLNAAGGAKWSI

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex)

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family

Homologues:

Organism=Escherichia coli, GI87082022, Length=375, Percent_Identity=29.0666666666667, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787062, Length=346, Percent_Identity=29.7687861271676, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786851, Length=269, Percent_Identity=32.3420074349442, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87082054, Length=213, Percent_Identity=30.9859154929577, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DACB_BACSU (P35150)

Other databases:

- EMBL:   M84227
- EMBL:   L09228
- EMBL:   AL009126
- PIR:   S45552
- RefSeq:   NP_390200.1
- PDB:   3MFD
- PDBsum:   3MFD
- ProteinModelPortal:   P35150
- SMR:   P35150
- MEROPS:   S11.004
- EnsemblBacteria:   EBBACT00000003006
- GeneID:   938953
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23190
- NMPDR:   fig|224308.1.peg.2323
- GenoList:   BSU23190
- GeneTree:   EBGT00070000031758
- HOGENOM:   HBG728991
- OMA:   KLMNEYA
- PhylomeDB:   P35150
- ProtClustDB:   CLSK2752437
- BioCyc:   BSUB:BSU23190-MONOMER
- BRENDA:   3.4.16.4
- GO:   GO:0016020
- GO:   GO:0006508
- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967
- PRINTS:   PR00725

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11; SSF56601 PBP_transp_fold; SSF69189 Peniciliin-bd_prot-assoc

EC number: =3.4.16.4

Molecular weight: Translated: 43081; Mature: 43081

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: ACT_SITE 60-60 ACT_SITE 63-63 ACT_SITE 115-115 BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIFKKAVFVIMISFLIATVNVNTAHAAIDVSAKSAIIIDGASGRVLYAKDEHQKRRIAS
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCEEEECCHHHHHHHHH
ITKIMTAVLAIESGKMDQTVTVSANAVRTEGSAIYLTEGQKVKLKDLVYGLMLRSGNDAA
HHHHHHHHHHHCCCCCCCEEEEECCEEEECCCEEEEECCCEEEHHHHHHHHHHCCCCCCE
VAIAEHVGGSLDGFVYMMNQKAEQLGMKNTRFQNPHGLDDHENHYSTAYDMAILTKYAMK
EEEHHHCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LKDYQKISGTKIYKAETMESVWKNKNKLLTMLYPYSTGGKTGYTKLAKRTLVSTASKDGI
HHHHHHCCCCEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCC
DLIAVTINDPNDWDDHMKMFNYVFEHYQTYLIAKKGDIPKLKGTFYESKAFIKRDITYLL
EEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHCEEEEE
TEEEKENVKINTTLLKPKKAWEKDASKIPDIVGHMEIMFNDATIAKVPIYYENERHQKPK
ECCCCCCEEEEEEEECCHHHHHHHHHHCHHHHCCEEEEECCCEEEEEEEEECCCCCCCHH
KQFFETFKSIFLNAAGGAKWSI
HHHHHHHHHHHEECCCCCCCCC
>Mature Secondary Structure
MRIFKKAVFVIMISFLIATVNVNTAHAAIDVSAKSAIIIDGASGRVLYAKDEHQKRRIAS
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCEEEECCHHHHHHHHH
ITKIMTAVLAIESGKMDQTVTVSANAVRTEGSAIYLTEGQKVKLKDLVYGLMLRSGNDAA
HHHHHHHHHHHCCCCCCCEEEEECCEEEECCCEEEEECCCEEEHHHHHHHHHHCCCCCCE
VAIAEHVGGSLDGFVYMMNQKAEQLGMKNTRFQNPHGLDDHENHYSTAYDMAILTKYAMK
EEEHHHCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LKDYQKISGTKIYKAETMESVWKNKNKLLTMLYPYSTGGKTGYTKLAKRTLVSTASKDGI
HHHHHHCCCCEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCC
DLIAVTINDPNDWDDHMKMFNYVFEHYQTYLIAKKGDIPKLKGTFYESKAFIKRDITYLL
EEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHCEEEEE
TEEEKENVKINTTLLKPKKAWEKDASKIPDIVGHMEIMFNDATIAKVPIYYENERHQKPK
ECCCCCCEEEEEEEECCHHHHHHHHHHCHHHHCCEEEEECCCEEEEEEEEECCCCCCCHH
KQFFETFKSIFLNAAGGAKWSI
HHHHHHHHHHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377