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The map label for this gene is ypsC

Identifier: 16079275

GI number: 16079275

Start: 2330075

End: 2331232

Strand: Reverse

Name: ypsC

Synonym: BSU22170

Alternate gene names: 16079275

Gene position: 2331232-2330075 (Counterclockwise)

Preceding gene: 16079276

Following gene: 16079274

Centisome position: 55.3

GC content: 45.08

Gene sequence:

>1158_bases
ATGAAGAAGTATACACTAATTGCAACGGCGCCGATGGGCATTGAAGCTGTTGTCGCAAAGGAAGTACGAGATTTAGGATA
CGAATGCAAGGTTGATAACGGGAAAGTTATTTTTGAAGGTGATGCACTTGCCATCTGCCGTGCGAACCTTTGGCTTAGAA
CAGCCGACCGCATAAAGGTTCAGGTTGCTTCTTTTAAAGCGAAAACATTTGATGAACTGTTTGAAAAAACGAAAGCGATT
AACTGGCGCTCATTTATACCTGAAAACGGGAAATTCCCTGTCATCGGAAAATCGGTAAAATCAACACTTGCAAGCGTTCC
TGACTGTCAGCGGATCGTCAAAAAAGCAATTGTCGAAAAGCTCAAGCTGCAGTCCGGCAAAGCCAATGATTGGATTGAAG
AAACAGGTGCTGAGTATAAAGTGGAAATATCTTTATTGAAAGATCAGGCTTTGATTACACTGGACTCATCAGGGACGGGC
CTTCACAAACGAGGATACCGTGTCGATCAGGGCGGTGCGCCGATTAAAGAAACACTTGCTGCCGCTTTGGTGCAATTAAC
CAATTGGACGCCTGACCGCCCGTTTGTTGATCCTTTCTGCGGCTCCGGAACAATCGCGATAGAAGCGGCGCTAATCGGCC
AAAACATCGCCCCGGGATTCAACCGCGATTTTGTATCAGAGGATTGGGAATGGATCGGTAAAGATCTGTGGAACAAAGCG
CGGCTTGAAGTCGAAGAAAAGGCAAATTATGATCAGCCGCTTACAATTTTCGCTAGCGACATAGACCACCGCATGGTTCA
AATCGCAAAAGAAAATGCAGAGGAAGCAGGATTGGGAGATCTAATCCAGTTCAAACAGATGCAGGTCAAAGACTTTACCA
CAAATCTGGAATTTGGCGTAATTGTCGGCAACCCGCCATATGGAGAGCGTCTCGGCGAGAAAAAAGCCGTTGAGCAGATG
TACAAAGAAATGGGGCAGGCGTTCGAGCCGCTTGATACTTGGTCTGTGTATATGCTGACATCAAATGAAAACTTTGAGGA
AGCATATGGCAGAAAAGCAACGAAGAAACGAAAACTCTTTAACGGGTTTATTAAAACGGATTATTATCAATACTGGTCAA
AAGTAAGACCGCAGCGCAAGAAAACAGAAAACGCATAA

Upstream 100 bases:

>100_bases
CGTTAGACCACTTACATTTAAAATGATGAAAACAAGCTCTCCCGTATAAGGAGAGCTTTTATCTTGAAAAGAGAAAAGTT
TTAAAAGACAGGGTGATACG

Downstream 100 bases:

>100_bases
AAAGAAGACGCTCTGCATGCGTCTTCTTTCATCTTTCAGCCCTATTCTATCGTTACCAACGGTGAGCTTTCGCGTTGCTT
CTCAATATAATGTTGGTTTG

Product: methylase with RNA interaction domain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKKYTLIATAPMGIEAVVAKEVRDLGYECKVDNGKVIFEGDALAICRANLWLRTADRIKVQVASFKAKTFDELFEKTKAI
NWRSFIPENGKFPVIGKSVKSTLASVPDCQRIVKKAIVEKLKLQSGKANDWIEETGAEYKVEISLLKDQALITLDSSGTG
LHKRGYRVDQGGAPIKETLAAALVQLTNWTPDRPFVDPFCGSGTIAIEAALIGQNIAPGFNRDFVSEDWEWIGKDLWNKA
RLEVEEKANYDQPLTIFASDIDHRMVQIAKENAEEAGLGDLIQFKQMQVKDFTTNLEFGVIVGNPPYGERLGEKKAVEQM
YKEMGQAFEPLDTWSVYMLTSNENFEEAYGRKATKKRKLFNGFIKTDYYQYWSKVRPQRKKTENA

Sequences:

>Translated_385_residues
MKKYTLIATAPMGIEAVVAKEVRDLGYECKVDNGKVIFEGDALAICRANLWLRTADRIKVQVASFKAKTFDELFEKTKAI
NWRSFIPENGKFPVIGKSVKSTLASVPDCQRIVKKAIVEKLKLQSGKANDWIEETGAEYKVEISLLKDQALITLDSSGTG
LHKRGYRVDQGGAPIKETLAAALVQLTNWTPDRPFVDPFCGSGTIAIEAALIGQNIAPGFNRDFVSEDWEWIGKDLWNKA
RLEVEEKANYDQPLTIFASDIDHRMVQIAKENAEEAGLGDLIQFKQMQVKDFTTNLEFGVIVGNPPYGERLGEKKAVEQM
YKEMGQAFEPLDTWSVYMLTSNENFEEAYGRKATKKRKLFNGFIKTDYYQYWSKVRPQRKKTENA
>Mature_385_residues
MKKYTLIATAPMGIEAVVAKEVRDLGYECKVDNGKVIFEGDALAICRANLWLRTADRIKVQVASFKAKTFDELFEKTKAI
NWRSFIPENGKFPVIGKSVKSTLASVPDCQRIVKKAIVEKLKLQSGKANDWIEETGAEYKVEISLLKDQALITLDSSGTG
LHKRGYRVDQGGAPIKETLAAALVQLTNWTPDRPFVDPFCGSGTIAIEAALIGQNIAPGFNRDFVSEDWEWIGKDLWNKA
RLEVEEKANYDQPLTIFASDIDHRMVQIAKENAEEAGLGDLIQFKQMQVKDFTTNLEFGVIVGNPPYGERLGEKKAVEQM
YKEMGQAFEPLDTWSVYMLTSNENFEEAYGRKATKKRKLFNGFIKTDYYQYWSKVRPQRKKTENA

Specific function: Unknown

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=343, Percent_Identity=32.3615160349854, Blast_Score=183, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YPSC_BACSU (P50840)

Other databases:

- EMBL:   L47838
- EMBL:   AL009126
- EMBL:   M13175
- PIR:   F69941
- RefSeq:   NP_390099.1
- ProteinModelPortal:   P50840
- SMR:   P50840
- EnsemblBacteria:   EBBACT00000003834
- GeneID:   939057
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22170
- NMPDR:   fig|224308.1.peg.2222
- GenoList:   BSU22170
- GeneTree:   EBGT00050000002845
- HOGENOM:   HBG452772
- OMA:   CNPPYGK
- ProtClustDB:   CLSK887484
- BioCyc:   BSUB:BSU22170-MONOMER
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR004114
- SMART:   SM00981

Pfam domain/function: PF02926 THUMP; PF01170 UPF0020

EC number: NA

Molecular weight: Translated: 43507; Mature: 43507

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00092 N6_MTASE; PS51165 THUMP; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYTLIATAPMGIEAVVAKEVRDLGYECKVDNGKVIFEGDALAICRANLWLRTADRIKV
CCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCEEEEECCEEEEEECCCEEEECCCEEE
QVASFKAKTFDELFEKTKAINWRSFIPENGKFPVIGKSVKSTLASVPDCQRIVKKAIVEK
EEEHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCEECHHHHHHHHCCHHHHHHHHHHHHHH
LKLQSGKANDWIEETGAEYKVEISLLKDQALITLDSSGTGLHKRGYRVDQGGAPIKETLA
HHHCCCCCCCHHHHCCCCEEEEEEEECCCEEEEECCCCCCCCCCCCEECCCCCCHHHHHH
AALVQLTNWTPDRPFVDPFCGSGTIAIEAALIGQNIAPGFNRDFVSEDWEWIGKDLWNKA
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RLEVEEKANYDQPLTIFASDIDHRMVQIAKENAEEAGLGDLIQFKQMQVKDFTTNLEFGV
HEEEHHHCCCCCCEEEEECHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHCCCCEEEE
IVGNPPYGERLGEKKAVEQMYKEMGQAFEPLDTWSVYMLTSNENFEEAYGRKATKKRKLF
EECCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHCCHHHHHHHHH
NGFIKTDYYQYWSKVRPQRKKTENA
HHHHHHHHHHHHHHHCCHHHCCCCC
>Mature Secondary Structure
MKKYTLIATAPMGIEAVVAKEVRDLGYECKVDNGKVIFEGDALAICRANLWLRTADRIKV
CCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCEEEEECCEEEEEECCCEEEECCCEEE
QVASFKAKTFDELFEKTKAINWRSFIPENGKFPVIGKSVKSTLASVPDCQRIVKKAIVEK
EEEHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCEECHHHHHHHHCCHHHHHHHHHHHHHH
LKLQSGKANDWIEETGAEYKVEISLLKDQALITLDSSGTGLHKRGYRVDQGGAPIKETLA
HHHCCCCCCCHHHHCCCCEEEEEEEECCCEEEEECCCCCCCCCCCCEECCCCCCHHHHHH
AALVQLTNWTPDRPFVDPFCGSGTIAIEAALIGQNIAPGFNRDFVSEDWEWIGKDLWNKA
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RLEVEEKANYDQPLTIFASDIDHRMVQIAKENAEEAGLGDLIQFKQMQVKDFTTNLEFGV
HEEEHHHCCCCCCEEEEECHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHCCCCEEEE
IVGNPPYGERLGEKKAVEQMYKEMGQAFEPLDTWSVYMLTSNENFEEAYGRKATKKRKLF
EECCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHCCHHHHHHHHH
NGFIKTDYYQYWSKVRPQRKKTENA
HHHHHHHHHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 2423526