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The map label for this gene is ilvA

Identifier: 16079236

GI number: 16079236

Start: 2292769

End: 2294037

Strand: Reverse

Name: ilvA

Synonym: BSU21770

Alternate gene names: 16079236

Gene position: 2294037-2292769 (Counterclockwise)

Preceding gene: 255767477

Following gene: 16079235

Centisome position: 54.42

GC content: 44.05

Gene sequence:

>1269_bases
ATGAAACCGTTGCTTAAAGAAAACTCTCTCATCCAAGTGAAAGACATTTTGAAGGCTCACCAAAACGTAAAGGACGTTGT
CATTCACACACCTTTGCAGAGGAATGACAGACTTTCTGAGAGATATGAATGCAATATCTATTTAAAACGCGAAGACTTGC
AAGTTGTCAGATCTTTTAAGCTCAGAGGGGCTTATCATAAAATGAAACAGCTTTCCAGCGAGCAGACAGAGAACGGAGTT
GTGTGTGCAAGTGCCGGAAACCACGCTCAAGGTGTGGCATTTTCGTGTAAACATCTTGGCATTCACGGGAAAATTTTTAT
GCCGTCTACTACACCGAGACAAAAAGTATCTCAAGTTGAACTATTCGGAAAAGGATTTATCGATATTATTTTGACAGGCG
ATACGTTTGACGACGCGTATAAAAGCGCTGCTGAATGCTGTGAGGCGGAATCTCGGACGTTTATCCATCCGTTCGATGAT
CCGGATGTGATGGCCGGACAGGGAACGCTCGCAGTCGAGATTTTAAACGACATTGATACAGAACCGCATTTTCTTTTTGC
AAGTGTCGGAGGCGGCGGCCTTCTCTCTGGGGTAGGTACATATTTGAAAAACGTGTCTCCTGATACAAAGGTAATTGCTG
TTGAGCCGGCGGGAGCGGCATCCTACTTTGAATCAAACAAAGCTGGGCATGTCGTCACACTTGATAAAATTGATAAGTTT
GTAGATGGGGCGGCTGTTAAAAAAATTGGCGAGGAAACCTTCCGGACGCTGGAAACTGTCGTTGATGACATTCTCCTTGT
GCCTGAAGGAAAAGTATGTACATCCATTCTTGAATTATATAACGAATGTGCGGTTGTTGCTGAACCGGCTGGAGCTCTTT
CTGTCGCAGCGCTTGATTTGTACAAGGATCAAATTAAAGGGAAAAACGTGGTGTGTGTGGTAAGCGGGGGCAATAACGAT
ATCGGAAGAATGCAGGAAATGAAGGAGCGTTCCTTGATTTTTGAAGGCCTTCAGCATTATTTTATCGTCAATTTTCCACA
AAGAGCGGGGGCGCTCCGCGAATTTCTTGACGAAGTCCTCGGGCCGAATGATGACATTACAAGGTTTGAATATACGAAGA
AAAATAATAAAAGCAATGGACCTGCTCTTGTCGGCATCGAGCTGCAGAACAAAGCCGATTATGGGCCGCTGATTGAACGG
ATGAACAAAAAACCGTTTCATTATGTAGAGGTGAACAAGGATGAGGACTTGTTCCATTTGCTAATCTAA

Upstream 100 bases:

>100_bases
TTTTTTTAATTATTAAATAAAAATAATTGTCTTATTTTTTGAATATTCATGTTATAATGGCATATTAAATAGGGATTTAA
AACAAGAAAGGAATCTGTAC

Downstream 100 bases:

>100_bases
TTTTTTTACACAACCGCGCAAAATGACAGACAGCCCGGCTGGCATCATATATAATAGAAGAAAAACAAAAAAGAGGATTG
ATAGCTTGTTACTCAAAAGC

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKPLLKENSLIQVKDILKAHQNVKDVVIHTPLQRNDRLSERYECNIYLKREDLQVVRSFKLRGAYHKMKQLSSEQTENGV
VCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKVSQVELFGKGFIDIILTGDTFDDAYKSAAECCEAESRTFIHPFDD
PDVMAGQGTLAVEILNDIDTEPHFLFASVGGGGLLSGVGTYLKNVSPDTKVIAVEPAGAASYFESNKAGHVVTLDKIDKF
VDGAAVKKIGEETFRTLETVVDDILLVPEGKVCTSILELYNECAVVAEPAGALSVAALDLYKDQIKGKNVVCVVSGGNND
IGRMQEMKERSLIFEGLQHYFIVNFPQRAGALREFLDEVLGPNDDITRFEYTKKNNKSNGPALVGIELQNKADYGPLIER
MNKKPFHYVEVNKDEDLFHLLI

Sequences:

>Translated_422_residues
MKPLLKENSLIQVKDILKAHQNVKDVVIHTPLQRNDRLSERYECNIYLKREDLQVVRSFKLRGAYHKMKQLSSEQTENGV
VCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKVSQVELFGKGFIDIILTGDTFDDAYKSAAECCEAESRTFIHPFDD
PDVMAGQGTLAVEILNDIDTEPHFLFASVGGGGLLSGVGTYLKNVSPDTKVIAVEPAGAASYFESNKAGHVVTLDKIDKF
VDGAAVKKIGEETFRTLETVVDDILLVPEGKVCTSILELYNECAVVAEPAGALSVAALDLYKDQIKGKNVVCVVSGGNND
IGRMQEMKERSLIFEGLQHYFIVNFPQRAGALREFLDEVLGPNDDITRFEYTKKNNKSNGPALVGIELQNKADYGPLIER
MNKKPFHYVEVNKDEDLFHLLI
>Mature_422_residues
MKPLLKENSLIQVKDILKAHQNVKDVVIHTPLQRNDRLSERYECNIYLKREDLQVVRSFKLRGAYHKMKQLSSEQTENGV
VCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKVSQVELFGKGFIDIILTGDTFDDAYKSAAECCEAESRTFIHPFDD
PDVMAGQGTLAVEILNDIDTEPHFLFASVGGGGLLSGVGTYLKNVSPDTKVIAVEPAGAASYFESNKAGHVVTLDKIDKF
VDGAAVKKIGEETFRTLETVVDDILLVPEGKVCTSILELYNECAVVAEPAGALSVAALDLYKDQIKGKNVVCVVSGGNND
IGRMQEMKERSLIFEGLQHYFIVNFPQRAGALREFLDEVLGPNDDITRFEYTKKNNKSNGPALVGIELQNKADYGPLIER
MNKKPFHYVEVNKDEDLFHLLI

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=318, Percent_Identity=30.8176100628931, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI33469958, Length=315, Percent_Identity=28.2539682539683, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI19923959, Length=310, Percent_Identity=29.3548387096774, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1790207, Length=389, Percent_Identity=38.560411311054, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1789505, Length=320, Percent_Identity=36.5625, Blast_Score=184, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI71991565, Length=349, Percent_Identity=33.2378223495702, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17537387, Length=347, Percent_Identity=32.5648414985591, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17508781, Length=314, Percent_Identity=26.4331210191083, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320930, Length=376, Percent_Identity=38.563829787234, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322631, Length=309, Percent_Identity=35.2750809061489, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319788, Length=281, Percent_Identity=24.1992882562278, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21355833, Length=346, Percent_Identity=32.0809248554913, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24645328, Length=250, Percent_Identity=31.2, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 46652; Mature: 46652

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLLKENSLIQVKDILKAHQNVKDVVIHTPLQRNDRLSERYECNIYLKREDLQVVRSFK
CCCCCCCCCEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEEEHHHHHHHHHHH
LRGAYHKMKQLSSEQTENGVVCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKVSQVE
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHH
LFGKGFIDIILTGDTFDDAYKSAAECCEAESRTFIHPFDDPDVMAGQGTLAVEILNDIDT
HHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCEEEEEHHHCCC
EPHFLFASVGGGGLLSGVGTYLKNVSPDTKVIAVEPAGAASYFESNKAGHVVTLDKIDKF
CCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCCCCEEEHHHHHHH
VDGAAVKKIGEETFRTLETVVDDILLVPEGKVCTSILELYNECAVVAEPAGALSVAALDL
HCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHH
YKDQIKGKNVVCVVSGGNNDIGRMQEMKERSLIFEGLQHYFIVNFPQRAGALREFLDEVL
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHC
GPNDDITRFEYTKKNNKSNGPALVGIELQNKADYGPLIERMNKKPFHYVEVNKDEDLFHL
CCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCEEEEECCCCCCCEE
LI
CC
>Mature Secondary Structure
MKPLLKENSLIQVKDILKAHQNVKDVVIHTPLQRNDRLSERYECNIYLKREDLQVVRSFK
CCCCCCCCCEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEEEHHHHHHHHHHH
LRGAYHKMKQLSSEQTENGVVCASAGNHAQGVAFSCKHLGIHGKIFMPSTTPRQKVSQVE
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHH
LFGKGFIDIILTGDTFDDAYKSAAECCEAESRTFIHPFDDPDVMAGQGTLAVEILNDIDT
HHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCEEEEEHHHCCC
EPHFLFASVGGGGLLSGVGTYLKNVSPDTKVIAVEPAGAASYFESNKAGHVVTLDKIDKF
CCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCCCCEEEHHHHHHH
VDGAAVKKIGEETFRTLETVVDDILLVPEGKVCTSILELYNECAVVAEPAGALSVAALDL
HCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHH
YKDQIKGKNVVCVVSGGNNDIGRMQEMKERSLIFEGLQHYFIVNFPQRAGALREFLDEVL
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHC
GPNDDITRFEYTKKNNKSNGPALVGIELQNKADYGPLIERMNKKPFHYVEVNKDEDLFHL
CCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCEEEEECCCCCCCEE
LI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]