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The map label for this gene is mtbP

Identifier: 16079084

GI number: 16079084

Start: 2171401

End: 2172732

Strand: Reverse

Name: mtbP

Synonym: BSU20250

Alternate gene names: 16079084

Gene position: 2172732-2171401 (Counterclockwise)

Preceding gene: 16079085

Following gene: 16079083

Centisome position: 51.54

GC content: 36.19

Gene sequence:

>1332_bases
TTGAGTAAACTACGTGTAATGAGCCTTTTTAGTGGAATTGGTGCATTTGAAGCTGCATTAAGAAACATAGGCGTTGATTA
TGAACTGATTGGCTTTAGTGAAATAGATAAATATGCAATCAAATCTTATTGTGCAATTCATAATGTAAGTGAAACATTGA
ATGTTGGAGACATAAGTAAAGCTAAGAAAGATAATATTCCATATTTTGATTTGTTAACAAGTGGATTTCCTTGTCCCACT
TTTTCAGTAGCCGGCGGCCGTGATGGTATGGAATATAAGTGTAGCAATTGTTCGCACGAGCATTTAATTACTTATGAGGA
TTATAAGAAGGGAGTCAAATGCCCGAAGTGTGAAGCTGTTTCTAAGGCTAAAGATGAACGGGGAACGCTCTTTTTTGAAA
CAGCTTTGTTAGCAGAAGAGAAGAAGCCGAAATTTGTGATATTAGAAAATGTAAAAGGGCTAATTAATAGTGGCAATGGA
CAAGTGTTAAGAATAATTAGTGAGACTATGAACAATATAGGCTATAGGATTGACCTAGAGCTACTTAATTCAAAGTTTTT
CAATGTTCCACAGAATCGTGAACGGGTATACATAATTGGCATTAGAGAAGATTTGGTTGAAAATGAACAATGGGTTGTAG
GTCAGAAGCGAAACGATGTTCTGAGTAAAGGTAAAAAGAGATTGCAAGAAATAAATATAAAGAGTTTTAATTTTAAATGG
CCTTTACAAGACACTGTCACAAAGAGGTTGAGAGAAATTCTTGAGGATTTTGTTGATGAGAAGTATTACTTAAATGAAGA
AAAGACGAAGAAACTTGTTGAGCAACTAGGAACCGCACCGCTACAAAAACAAGAAGTAAGGGAGCCGTTAATGGTGGGAC
ACGTGGATTTAAAAGGTCACGATGCCATCAAAAGAGTTTACTCGCCTGAAGGACTTTCACCGACATTAACTACAATGGGC
GGCGGTCACAGAGAACCTAAAATCGCAGAAAAGCAGAAAGAAGTAAGGGCAGTGTTAACGCCAGAAAGAGAAGAGAAAAG
ACAGAATGGAAGACGTTTTAAAGAAAACGGAGAACCGGCTTTCACGGTTAATACAATTGATCGACATGGTGTAGCAATCG
GAGAATACCCAAAATATAAAATTAGAAAGCTCTCTCCATTAGAATGTTGGAGACTCCAAGCATTTGATGACGAAGATTTT
GAAAAAGCTTTTGCAGCAGGAATAAGTAACTCACAATTATACAAGCAAGCCGGTAATTCAATTACAGTTAGTGTGCTTGA
GTCTATATTTCAAGAATTAATACATACATATGTTAATAAAGAATCTGAATAA

Upstream 100 bases:

>100_bases
TATGAACGAAGCAAAAACAGAGTTTGAAAAAGTGCTAAAGAAATTAAAATAAAAGATCAATTTTATTTAGAGTAAAAATA
AAATATATGGAGGTTGTTTG

Downstream 100 bases:

>100_bases
AATTTGTCTTTTAAACAAATATAAAATAAGAGGAGTGGATCGATTGGATAGTTACCCAGAGTCCCTAAAAAAAGAGACAG
AGGAGATTAAAGAGCGTGTT

Product: SP-beta prophage DNA (cytosine-5-)-methyltransferase

Products: NA

Alternate protein names: M.Phi3TI; Cytosine-specific methyltransferase Phi3TI

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSKLRVMSLFSGIGAFEAALRNIGVDYELIGFSEIDKYAIKSYCAIHNVSETLNVGDISKAKKDNIPYFDLLTSGFPCPT
FSVAGGRDGMEYKCSNCSHEHLITYEDYKKGVKCPKCEAVSKAKDERGTLFFETALLAEEKKPKFVILENVKGLINSGNG
QVLRIISETMNNIGYRIDLELLNSKFFNVPQNRERVYIIGIREDLVENEQWVVGQKRNDVLSKGKKRLQEINIKSFNFKW
PLQDTVTKRLREILEDFVDEKYYLNEEKTKKLVEQLGTAPLQKQEVREPLMVGHVDLKGHDAIKRVYSPEGLSPTLTTMG
GGHREPKIAEKQKEVRAVLTPEREEKRQNGRRFKENGEPAFTVNTIDRHGVAIGEYPKYKIRKLSPLECWRLQAFDDEDF
EKAFAAGISNSQLYKQAGNSITVSVLESIFQELIHTYVNKESE

Sequences:

>Translated_443_residues
MSKLRVMSLFSGIGAFEAALRNIGVDYELIGFSEIDKYAIKSYCAIHNVSETLNVGDISKAKKDNIPYFDLLTSGFPCPT
FSVAGGRDGMEYKCSNCSHEHLITYEDYKKGVKCPKCEAVSKAKDERGTLFFETALLAEEKKPKFVILENVKGLINSGNG
QVLRIISETMNNIGYRIDLELLNSKFFNVPQNRERVYIIGIREDLVENEQWVVGQKRNDVLSKGKKRLQEINIKSFNFKW
PLQDTVTKRLREILEDFVDEKYYLNEEKTKKLVEQLGTAPLQKQEVREPLMVGHVDLKGHDAIKRVYSPEGLSPTLTTMG
GGHREPKIAEKQKEVRAVLTPEREEKRQNGRRFKENGEPAFTVNTIDRHGVAIGEYPKYKIRKLSPLECWRLQAFDDEDF
EKAFAAGISNSQLYKQAGNSITVSVLESIFQELIHTYVNKESE
>Mature_442_residues
SKLRVMSLFSGIGAFEAALRNIGVDYELIGFSEIDKYAIKSYCAIHNVSETLNVGDISKAKKDNIPYFDLLTSGFPCPTF
SVAGGRDGMEYKCSNCSHEHLITYEDYKKGVKCPKCEAVSKAKDERGTLFFETALLAEEKKPKFVILENVKGLINSGNGQ
VLRIISETMNNIGYRIDLELLNSKFFNVPQNRERVYIIGIREDLVENEQWVVGQKRNDVLSKGKKRLQEINIKSFNFKWP
LQDTVTKRLREILEDFVDEKYYLNEEKTKKLVEQLGTAPLQKQEVREPLMVGHVDLKGHDAIKRVYSPEGLSPTLTTMGG
GHREPKIAEKQKEVRAVLTPEREEKRQNGRRFKENGEPAFTVNTIDRHGVAIGEYPKYKIRKLSPLECWRLQAFDDEDFE
KAFAAGISNSQLYKQAGNSITVSVLESIFQELIHTYVNKESE

Specific function: This enzyme methylates the first cytosine within the sequences GGCC and GCNGC

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family

Homologues:

Organism=Escherichia coli, GI1788271, Length=229, Percent_Identity=29.2576419213974, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTBP_BACSU (P68585)

Other databases:

- EMBL:   AL009126
- RefSeq:   NP_389907.1
- ProteinModelPortal:   P68585
- SMR:   P68585
- REBASE:   3140
- EnsemblBacteria:   EBBACT00000001747
- GeneID:   939532
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU20250
- NMPDR:   fig|224308.1.peg.2030
- GenoList:   BSU20250
- GeneTree:   EBGT00050000004517
- HOGENOM:   HBG682358
- OMA:   WHSDITT
- PhylomeDB:   P68585
- ProtClustDB:   CLSK887444
- BioCyc:   BSUB:BSU20250-MONOMER
- BRENDA:   2.1.1.37
- InterPro:   IPR018117
- InterPro:   IPR001525
- PANTHER:   PTHR10629
- PRINTS:   PR00105
- TIGRFAMs:   TIGR00675

Pfam domain/function: PF00145 DNA_methylase

EC number: =2.1.1.37

Molecular weight: Translated: 50510; Mature: 50379

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00094 C5_MTASE_1; PS00095 C5_MTASE_2

Important sites: ACT_SITE 78-78

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLRVMSLFSGIGAFEAALRNIGVDYELIGFSEIDKYAIKSYCAIHNVSETLNVGDISK
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AKKDNIPYFDLLTSGFPCPTFSVAGGRDGMEYKCSNCSHEHLITYEDYKKGVKCPKCEAV
HHHCCCCCHHHHHCCCCCCCEECCCCCCCCCEECCCCCCCCEEEEHHHHCCCCCCCHHHH
SKAKDERGTLFFETALLAEEKKPKFVILENVKGLINSGNGQVLRIISETMNNIGYRIDLE
HHCCCCCCEEEEEHHHHHHCCCCCEEEEECCHHHHCCCCCCEEHHHHHHHHHCCEEEEEE
LLNSKFFNVPQNRERVYIIGIREDLVENEQWVVGQKRNDVLSKGKKRLQEINIKSFNFKW
ECCCHHCCCCCCCCEEEEEEEHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEEEC
PLQDTVTKRLREILEDFVDEKYYLNEEKTKKLVEQLGTAPLQKQEVREPLMVGHVDLKGH
CHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHCCCCCCHHHHCCCCEEEEECCCCH
DAIKRVYSPEGLSPTLTTMGGGHREPKIAEKQKEVRAVLTPEREEKRQNGRRFKENGEPA
HHHHHHCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCE
FTVNTIDRHGVAIGEYPKYKIRKLSPLECWRLQAFDDEDFEKAFAAGISNSQLYKQAGNS
EEEECCHHCCCEECCCCCCHHCCCCCCHHHEEECCCCHHHHHHHHHCCCCHHHHHHCCCE
ITVSVLESIFQELIHTYVNKESE
EEHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKLRVMSLFSGIGAFEAALRNIGVDYELIGFSEIDKYAIKSYCAIHNVSETLNVGDISK
CCHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AKKDNIPYFDLLTSGFPCPTFSVAGGRDGMEYKCSNCSHEHLITYEDYKKGVKCPKCEAV
HHHCCCCCHHHHHCCCCCCCEECCCCCCCCCEECCCCCCCCEEEEHHHHCCCCCCCHHHH
SKAKDERGTLFFETALLAEEKKPKFVILENVKGLINSGNGQVLRIISETMNNIGYRIDLE
HHCCCCCCEEEEEHHHHHHCCCCCEEEEECCHHHHCCCCCCEEHHHHHHHHHCCEEEEEE
LLNSKFFNVPQNRERVYIIGIREDLVENEQWVVGQKRNDVLSKGKKRLQEINIKSFNFKW
ECCCHHCCCCCCCCEEEEEEEHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEEEC
PLQDTVTKRLREILEDFVDEKYYLNEEKTKKLVEQLGTAPLQKQEVREPLMVGHVDLKGH
CHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHCCCCCCHHHHCCCCEEEEECCCCH
DAIKRVYSPEGLSPTLTTMGGGHREPKIAEKQKEVRAVLTPEREEKRQNGRRFKENGEPA
HHHHHHCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCE
FTVNTIDRHGVAIGEYPKYKIRKLSPLECWRLQAFDDEDFEKAFAAGISNSQLYKQAGNS
EEEECCHHCCCEECCCCCCHHCCCCCCHHHEEECCCCHHHHHHHHHCCCCHHHHHHCCCE
ITVSVLESIFQELIHTYVNKESE
EEHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377