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The map label for this gene is cwlS

Identifier: 16079001

GI number: 16079001

Start: 2115425

End: 2116669

Strand: Reverse

Name: cwlS

Synonym: BSU19410

Alternate gene names: 16079001

Gene position: 2116669-2115425 (Counterclockwise)

Preceding gene: 50812248

Following gene: 16079000

Centisome position: 50.21

GC content: 42.41

Gene sequence:

>1245_bases
ATGAAAAAGAAGATTGTAGCCGGCTTGGCTGTTTCTGCAGTTGTTGGGTCGTCGATGGCCGCAGCACCCGCGGAAGCAAA
AACGATAAAAGTAAAAAGCGGTGACTCTCTTTGGAAACTTTCCCGCCAGTATGATACGACAATATCAGCTCTGAAATCCG
AAAACAAATTGAAATCAACGGTGCTTTATGTCGGACAAAGTCTTAAGGTTCCTGAAAGCAGCAAAAAAAGCACGACATCC
CCATCTAATTCAAGCAAAACATCAACATACACAGTTGCATATGGTGATTCCCTATGGATGATTGCTAAAAATCATAAAAT
GAGTGTTTCTGAGCTTAAAAGCTTAAACAGCCTGAGCAGTGACCTGATTCGTCCGGGGCAAAAGCTTAAGATCAAAGGGA
CAAGCAGTTCAAGCGGATCAAATGGATCAAAGAAAAACAGCGGTTCCAATTCTTCAGGTTCTTCAAAAAGTACATATACG
GTCAAGCTTGGTGACTCACTGTGGAAAATCGCAAACAGCTTAAATATGACTGTAGCCGAATTAAAAACCTTAAATGGCTT
AACATCAGATACACTTTATCCTAAACAGGTGCTCAAAATAAAAGGAAGCAGTTCACCTAAGAACGGAAACAGCGGTTCCA
AAAAGCCGTCAAACTCCAATCCCTCAAAAACAACGACGTACAAGGTAAAAGCAGGCGATTCGCTGTGGAAAATTGCAAAT
CGGCTCGGAGTCACTGTCCAAAGCATCCGTGATAAAAACAATTTATCATCGGATGTGTTGCAAATCGGGCAGGTTCTGAC
TATTTCGGGAGCATCAAAATCTAATCCTTCAAATCCGACAAAGCCAACAAAGCCAAAAGATAACTCAGGGTCAAACATTC
AAATAGGTTCGAAGATTGACAGAATGATAACGGAAGCGAAAAAATATGTCGGAGTGCCTTACCGATGGGGCGGAAACACG
CCTGCCGGTTTTGATTGCAGCGGCTTCATTTATTACCTCATTAATAATGTATCATCTATATCAAGATTGAGTACGGCAGG
GTATTGGAATGTCATGCAGAAAGTCAGCCAGCCGTCAGTAGGAGACTTTGTCTTCTTTACCACATATAAATCCGGCCCGT
CTCACATGGGGATTTATCTAGGCGGCGGTGATTTTATTCATGCAAGCTCATCGGGCGTCGACATTTCCAATCTCAGCAAT
TCGTATTGGAAACAGCGGTACTTGGGCGCAAGAAGTTATTTTTAA

Upstream 100 bases:

>100_bases
TTTAACTTGCTGAAAAATTGACGATAAAACAGTTGTGTGCCATTTTTGGAAACAAAATACTAGCTGTTTTTGCAAAAAAA
CAGTTTAGGAGGTTGAATGA

Downstream 100 bases:

>100_bases
AAATGAGTATGACAAAAAGCCTTTTATAGGCTTTTTTGTTTATACCAGACAATGATTGACCACACTGTTAGAAAAAACAA
GGGGGAGACAGTATGCACCG

Product: peptidoglycan hydrolase

Products: NA

Alternate protein names: Cell wall lytic enzyme associated with cell separation

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKKKIVAGLAVSAVVGSSMAAAPAEAKTIKVKSGDSLWKLSRQYDTTISALKSENKLKSTVLYVGQSLKVPESSKKSTTS
PSNSSKTSTYTVAYGDSLWMIAKNHKMSVSELKSLNSLSSDLIRPGQKLKIKGTSSSSGSNGSKKNSGSNSSGSSKSTYT
VKLGDSLWKIANSLNMTVAELKTLNGLTSDTLYPKQVLKIKGSSSPKNGNSGSKKPSNSNPSKTTTYKVKAGDSLWKIAN
RLGVTVQSIRDKNNLSSDVLQIGQVLTISGASKSNPSNPTKPTKPKDNSGSNIQIGSKIDRMITEAKKYVGVPYRWGGNT
PAGFDCSGFIYYLINNVSSISRLSTAGYWNVMQKVSQPSVGDFVFFTTYKSGPSHMGIYLGGGDFIHASSSGVDISNLSN
SYWKQRYLGARSYF

Sequences:

>Translated_414_residues
MKKKIVAGLAVSAVVGSSMAAAPAEAKTIKVKSGDSLWKLSRQYDTTISALKSENKLKSTVLYVGQSLKVPESSKKSTTS
PSNSSKTSTYTVAYGDSLWMIAKNHKMSVSELKSLNSLSSDLIRPGQKLKIKGTSSSSGSNGSKKNSGSNSSGSSKSTYT
VKLGDSLWKIANSLNMTVAELKTLNGLTSDTLYPKQVLKIKGSSSPKNGNSGSKKPSNSNPSKTTTYKVKAGDSLWKIAN
RLGVTVQSIRDKNNLSSDVLQIGQVLTISGASKSNPSNPTKPTKPKDNSGSNIQIGSKIDRMITEAKKYVGVPYRWGGNT
PAGFDCSGFIYYLINNVSSISRLSTAGYWNVMQKVSQPSVGDFVFFTTYKSGPSHMGIYLGGGDFIHASSSGVDISNLSN
SYWKQRYLGARSYF
>Mature_414_residues
MKKKIVAGLAVSAVVGSSMAAAPAEAKTIKVKSGDSLWKLSRQYDTTISALKSENKLKSTVLYVGQSLKVPESSKKSTTS
PSNSSKTSTYTVAYGDSLWMIAKNHKMSVSELKSLNSLSSDLIRPGQKLKIKGTSSSSGSNGSKKNSGSNSSGSSKSTYT
VKLGDSLWKIANSLNMTVAELKTLNGLTSDTLYPKQVLKIKGSSSPKNGNSGSKKPSNSNPSKTTTYKVKAGDSLWKIAN
RLGVTVQSIRDKNNLSSDVLQIGQVLTISGASKSNPSNPTKPTKPKDNSGSNIQIGSKIDRMITEAKKYVGVPYRWGGNT
PAGFDCSGFIYYLINNVSSISRLSTAGYWNVMQKVSQPSVGDFVFFTTYKSGPSHMGIYLGGGDFIHASSSGVDISNLSN
SYWKQRYLGARSYF

Specific function: Probably functions as a cell separation enzyme in addition to lytE and lytF

COG id: COG1388

COG function: function code M; FOG: LysM repeat

Gene ontology:

Cell location: Cell surface. Note=Localized at cell separation sites and cell poles during late vegetative phase

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 LysM repeats

Homologues:

Organism=Escherichia coli, GI1787944, Length=134, Percent_Identity=35.0746268656716, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786421, Length=121, Percent_Identity=34.7107438016529, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788501, Length=129, Percent_Identity=37.2093023255814, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CWLS_BACSU (O31852)

Other databases:

- EMBL:   AF026147
- EMBL:   AL009126
- PIR:   A69907
- RefSeq:   NP_389823.1
- ProteinModelPortal:   O31852
- MEROPS:   C40.008
- EnsemblBacteria:   EBBACT00000000226
- GeneID:   939459
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU19410
- NMPDR:   fig|224308.1.peg.1946
- GenoList:   BSU19410
- GeneTree:   EBGT00070000031872
- HOGENOM:   HBG362075
- OMA:   DFIHASS
- ProtClustDB:   CLSK886953
- BioCyc:   BSUB:BSU19410-MONOMER
- BRENDA:   3.4.19.11
- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482
- SMART:   SM00257

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60

EC number: =3.4.19.11

Molecular weight: Translated: 44235; Mature: 44235

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIVAGLAVSAVVGSSMAAAPAEAKTIKVKSGDSLWKLSRQYDTTISALKSENKLKST
CCCCHHHHHHHHHHHCCCHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLYVGQSLKVPESSKKSTTSPSNSSKTSTYTVAYGDSLWMIAKNHKMSVSELKSLNSLSS
HHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHH
DLIRPGQKLKIKGTSSSSGSNGSKKNSGSNSSGSSKSTYTVKLGDSLWKIANSLNMTVAE
HHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHCCHHHHH
LKTLNGLTSDTLYPKQVLKIKGSSSPKNGNSGSKKPSNSNPSKTTTYKVKAGDSLWKIAN
HHHHCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHH
RLGVTVQSIRDKNNLSSDVLQIGQVLTISGASKSNPSNPTKPTKPKDNSGSNIQIGSKID
HHCCHHHHHHCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECHHHH
RMITEAKKYVGVPYRWGGNTPAGFDCSGFIYYLINNVSSISRLSTAGYWNVMQKVSQPSV
HHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GDFVFFTTYKSGPSHMGIYLGGGDFIHASSSGVDISNLSNSYWKQRYLGARSYF
CCEEEEEEECCCCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKIVAGLAVSAVVGSSMAAAPAEAKTIKVKSGDSLWKLSRQYDTTISALKSENKLKST
CCCCHHHHHHHHHHHCCCHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLYVGQSLKVPESSKKSTTSPSNSSKTSTYTVAYGDSLWMIAKNHKMSVSELKSLNSLSS
HHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHH
DLIRPGQKLKIKGTSSSSGSNGSKKNSGSNSSGSSKSTYTVKLGDSLWKIANSLNMTVAE
HHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHCCHHHHH
LKTLNGLTSDTLYPKQVLKIKGSSSPKNGNSGSKKPSNSNPSKTTTYKVKAGDSLWKIAN
HHHHCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHH
RLGVTVQSIRDKNNLSSDVLQIGQVLTISGASKSNPSNPTKPTKPKDNSGSNIQIGSKID
HHCCHHHHHHCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECHHHH
RMITEAKKYVGVPYRWGGNTPAGFDCSGFIYYLINNVSSISRLSTAGYWNVMQKVSQPSV
HHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GDFVFFTTYKSGPSHMGIYLGGGDFIHASSSGVDISNLSNSYWKQRYLGARSYF
CCEEEEEEECCCCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9734814; 9384377