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The map label for this gene is yoaD

Identifier: 16078917

GI number: 16078917

Start: 2024042

End: 2025076

Strand: Reverse

Name: yoaD

Synonym: BSU18560

Alternate gene names: 16078917

Gene position: 2025076-2024042 (Counterclockwise)

Preceding gene: 16078920

Following gene: 16078916

Centisome position: 48.04

GC content: 42.42

Gene sequence:

>1035_bases
ATGAAAAATACGATGAAAAGGATGTTTTGCAGCATGACGGTTTTGGTTACAGCACCGTACAACGAAGAAGGACGAAAAGA
GCTTGAAAACTTGTTTGGCTCAGTTGCTTATCAATCTTGGAAGGAACAAGGTAGGGCATATCGGGAGGATGAACTCATTC
AGCTGCTAAAAGCAACAAATGCGACAGGCTTGATTACTGAACTTGATCAGGTAACAGACAGCGTATTTGCTTCTGTTCCC
GAACTTTCATTTGTCGGTGTTTGCAGGGGAATGCCCTCAAATGTGGATGTGGCAGCTGCCTCAAAAAGGGGCATACCTGT
TTTTTATACACCAGGGCGGAATGCACAAGCCGTTGCCGAAATGTTTATTGGAAATGTGATTTCCTTTCTGCGCCATACCA
GCGCCTCGAATCAGTGGCTGAAGGATGGAGAGTGGGACAGTGATTACCTGCAGGCTTATGTCAAATTTAAAGGAAATGAG
CTAACAGGAAAAACGGTTGGCATGATTGGGTTCGGCGCAGTCGGGCAAAGGATTGCAAAACTGCTGACTGCGTTTGACTG
TAAGATAAAATATTACGATCCATACATTCAAGACGATCATCCGTTGTATGAAAAAGCATCTCTTAAAACGGTGTTTTCAG
ACAGCGATATCGTTTCTGTCCATCTGCCTCGCACTGAGGAAACACTCGGACTGATAGATCGGCAATATTTTGATCTGATG
AAAGAGAGTGCGATTTTCGTTAATACTTCAAGAGCAGTTGTTGTAAACCGTGAAGATCTTTTATTTGTATTAAAAGAACA
TAAAATCAGCGGTGCGATACTAGACGTTTTTTATCATGAACCTCCAGAGGAATCAGATTATGAATTGATTTCATTGCCTA
ATGTTCTGGCAACACCGCATCTCGCTGGAGCAACATTTGAAGTGGAGGATCATCACGTGACGATACTGAATAAAGCCCTC
AAAAAGTGGAAGGGTGAAAAAACTCTTAATATTCAGACAATGTATAACAAGGACGCACTGAAAACAGGGGGATAA

Upstream 100 bases:

>100_bases
AGCTTGAAAGATAGGAAAGCAAGGTTTATACCGGCGTCTGCCTGTAACAGAGCGCGCCTATATATGAATCTCTTTCCATC
TTCGGAAAGAGATTTTTTTT

Downstream 100 bases:

>100_bases
GAAAGGAGGCTAACAGAATGAAAAAACAAAAAGGCTATCTCGTTTTTGATATTGGCACAGGCAATGCGAGGGTAGCGGTC
GTGAGTGTAACTGGCAGTGT

Product: 2-hydroxyacid dehydrogenase

Products: 3-phosphohydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKNTMKRMFCSMTVLVTAPYNEEGRKELENLFGSVAYQSWKEQGRAYREDELIQLLKATNATGLITELDQVTDSVFASVP
ELSFVGVCRGMPSNVDVAAASKRGIPVFYTPGRNAQAVAEMFIGNVISFLRHTSASNQWLKDGEWDSDYLQAYVKFKGNE
LTGKTVGMIGFGAVGQRIAKLLTAFDCKIKYYDPYIQDDHPLYEKASLKTVFSDSDIVSVHLPRTEETLGLIDRQYFDLM
KESAIFVNTSRAVVVNREDLLFVLKEHKISGAILDVFYHEPPEESDYELISLPNVLATPHLAGATFEVEDHHVTILNKAL
KKWKGEKTLNIQTMYNKDALKTGG

Sequences:

>Translated_344_residues
MKNTMKRMFCSMTVLVTAPYNEEGRKELENLFGSVAYQSWKEQGRAYREDELIQLLKATNATGLITELDQVTDSVFASVP
ELSFVGVCRGMPSNVDVAAASKRGIPVFYTPGRNAQAVAEMFIGNVISFLRHTSASNQWLKDGEWDSDYLQAYVKFKGNE
LTGKTVGMIGFGAVGQRIAKLLTAFDCKIKYYDPYIQDDHPLYEKASLKTVFSDSDIVSVHLPRTEETLGLIDRQYFDLM
KESAIFVNTSRAVVVNREDLLFVLKEHKISGAILDVFYHEPPEESDYELISLPNVLATPHLAGATFEVEDHHVTILNKAL
KKWKGEKTLNIQTMYNKDALKTGG
>Mature_344_residues
MKNTMKRMFCSMTVLVTAPYNEEGRKELENLFGSVAYQSWKEQGRAYREDELIQLLKATNATGLITELDQVTDSVFASVP
ELSFVGVCRGMPSNVDVAAASKRGIPVFYTPGRNAQAVAEMFIGNVISFLRHTSASNQWLKDGEWDSDYLQAYVKFKGNE
LTGKTVGMIGFGAVGQRIAKLLTAFDCKIKYYDPYIQDDHPLYEKASLKTVFSDSDIVSVHLPRTEETLGLIDRQYFDLM
KESAIFVNTSRAVVVNREDLLFVLKEHKISGAILDVFYHEPPEESDYELISLPNVLATPHLAGATFEVEDHHVTILNKAL
KKWKGEKTLNIQTMYNKDALKTGG

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Homologues:

Organism=Homo sapiens, GI23308577, Length=267, Percent_Identity=32.2097378277154, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI145580578, Length=239, Percent_Identity=30.9623430962343, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI4557499, Length=239, Percent_Identity=30.9623430962343, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI4557497, Length=256, Percent_Identity=29.296875, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI61743967, Length=256, Percent_Identity=29.296875, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI145580575, Length=261, Percent_Identity=29.1187739463602, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI6912396, Length=234, Percent_Identity=32.4786324786325, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1789279, Length=294, Percent_Identity=30.2721088435374, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87082289, Length=254, Percent_Identity=31.1023622047244, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787645, Length=195, Percent_Identity=31.2820512820513, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081824, Length=152, Percent_Identity=32.2368421052632, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788660, Length=276, Percent_Identity=28.2608695652174, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=264, Percent_Identity=31.4393939393939, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=260, Percent_Identity=26.9230769230769, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322116, Length=319, Percent_Identity=31.974921630094, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320925, Length=319, Percent_Identity=31.974921630094, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324055, Length=230, Percent_Identity=30.4347826086957, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=279, Percent_Identity=25.089605734767, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574286, Length=260, Percent_Identity=34.2307692307692, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI19921140, Length=264, Percent_Identity=29.1666666666667, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45552429, Length=260, Percent_Identity=30.7692307692308, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28574284, Length=260, Percent_Identity=30.7692307692308, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585514, Length=260, Percent_Identity=30.7692307692308, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28574282, Length=260, Percent_Identity=30.7692307692308, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45551003, Length=260, Percent_Identity=30.7692307692308, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585516, Length=216, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24646446, Length=224, Percent_Identity=30.3571428571429, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24646448, Length=224, Percent_Identity=30.3571428571429, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24646452, Length=224, Percent_Identity=30.3571428571429, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24646450, Length=224, Percent_Identity=30.3571428571429, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI62472511, Length=224, Percent_Identity=30.3571428571429, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28571528, Length=312, Percent_Identity=29.4871794871795, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): YOAD_BACSU (O34815)

Other databases:

- EMBL:   AF027868
- EMBL:   AL009126
- PIR:   F69895
- RefSeq:   NP_389738.1
- HSSP:   O58320
- ProteinModelPortal:   O34815
- SMR:   O34815
- EnsemblBacteria:   EBBACT00000004061
- GeneID:   940109
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU18560
- NMPDR:   fig|224308.1.peg.1860
- GenoList:   BSU18560
- GeneTree:   EBGT00050000001763
- HOGENOM:   HBG731446
- OMA:   ALITEHD
- ProtClustDB:   CLSK887381
- BioCyc:   BSUB:BSU18560-MONOMER
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF20

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: 1.1.1.95

Molecular weight: Translated: 38630; Mature: 38630

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 251-251 ACT_SITE 280-280 ACT_SITE 300-300 BINDING 193-193 BINDING 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTMKRMFCSMTVLVTAPYNEEGRKELENLFGSVAYQSWKEQGRAYREDELIQLLKATN
CCHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
ATGLITELDQVTDSVFASVPELSFVGVCRGMPSNVDVAAASKRGIPVFYTPGRNAQAVAE
CCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCCHHHHHH
MFIGNVISFLRHTSASNQWLKDGEWDSDYLQAYVKFKGNELTGKTVGMIGFGAVGQRIAK
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCEECEEEHHHHHHHHHH
LLTAFDCKIKYYDPYIQDDHPLYEKASLKTVFSDSDIVSVHLPRTEETLGLIDRQYFDLM
HHHHHCCEEEEECCCCCCCCCCHHHHHHHEEECCCCEEEEECCCCHHHHHHHHHHHHHHH
KESAIFVNTSRAVVVNREDLLFVLKEHKISGAILDVFYHEPPEESDYELISLPNVLATPH
HCCEEEEECCEEEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCCCEEEECCCHHCCCC
LAGATFEVEDHHVTILNKALKKWKGEKTLNIQTMYNKDALKTGG
CCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKNTMKRMFCSMTVLVTAPYNEEGRKELENLFGSVAYQSWKEQGRAYREDELIQLLKATN
CCHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
ATGLITELDQVTDSVFASVPELSFVGVCRGMPSNVDVAAASKRGIPVFYTPGRNAQAVAE
CCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCCHHHHHH
MFIGNVISFLRHTSASNQWLKDGEWDSDYLQAYVKFKGNELTGKTVGMIGFGAVGQRIAK
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCEECEEEHHHHHHHHHH
LLTAFDCKIKYYDPYIQDDHPLYEKASLKTVFSDSDIVSVHLPRTEETLGLIDRQYFDLM
HHHHHCCEEEEECCCCCCCCCCHHHHHHHEEECCCCEEEEECCCCHHHHHHHHHHHHHHH
KESAIFVNTSRAVVVNREDLLFVLKEHKISGAILDVFYHEPPEESDYELISLPNVLATPH
HCCEEEEECCEEEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCCCEEEECCCHHCCCC
LAGATFEVEDHHVTILNKALKKWKGEKTLNIQTMYNKDALKTGG
CCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-phosphoglycerate; NAD+

Specific reaction: 3-phosphoglycerate + NAD+ = 3-phosphohydroxypyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377