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The map label for this gene is dacC

Identifier: 16078896

GI number: 16078896

Start: 1998340

End: 1999815

Strand: Reverse

Name: dacC

Synonym: BSU18350

Alternate gene names: 16078896

Gene position: 1999815-1998340 (Counterclockwise)

Preceding gene: 16078897

Following gene: 255767429

Centisome position: 47.44

GC content: 44.65

Gene sequence:

>1476_bases
ATGAAAAAAAGCATAAAGCTTTATGTTGCTGTTTTACTGCTTTTCGTCGTTGCTTCGGTGCCTTATATGCATCAGGCTGC
GCTTGCCGCTGAAAAACAAGATGCACTTTCCGGGCAGATCGATAAGATTTTAGCTGATCATCCTGCCCTCGAAGGCGCAA
TGGCCGGCATCACCGTTCGCTCTGCGGAAACAGGTGCTGTTCTATATGAACATTCAGGAGACACCCGAATGAGGCCTGCC
TCTTCTCTAAAGCTTTTGACAGCGGCCGCGGCACTTTCTGTCCTTGGCGAGAACTATTCTTTTACAACCGAGGTCCGGAC
GGATGGAACACTGAAAGGGAAAAAGCTGAATGGTAACCTTTATTTGAAAGGGAAAGGCGATCCGACACTTTTACCATCTG
ATTTTGACAAAATGGCTGAGATATTGAAACATTCCGGCGTAAAAGTAATTAAAGGCAATCTGATCGGTGATGATACGTGG
CATGATGACATGCGATTATCACCTGATATGCCTTGGAGCGATGAATACACATATTATGGCGCGCCAATCTCCGCTCTAAC
AGCGTCTCCAAACGAAGACTATGATGCAGGCACTGTGATTGTTGAGGTCACCCCAAATCAAAAAGAAGGGGAAGAGCCTG
CCGTGTCTGTCTCTCCGAAAACCGATTATATAACGATAAAGAACGATGCTAAAACGACTGCTGCCGGTTCAGAGAAAGAC
CTCACAATCGAAAGAGAGCATGGAACAAACACGATTACAATTGAAGGGAGCGTACCGGTTGACGCAAACAAGACAAAGGA
ATGGATTTCTGTCTGGGAGCCTGCCGGATATGCCTTAGATTTATTTAAGCAGTCACTAAAAAAACAAGGTATTACTGTGA
AAGGGGACATCAAGACGGGTGAAGCACCTAGTTCTTCAGATGTGCTGCTCTCTCATCGCTCAATGCCTTTATCGAAACTT
TTTGTGCCGTTCATGAAATTAAGTAATAACGGACATGCCGAAGTGCTCGTAAAAGAGATGGGGAAAGTGAAAAAAGGAGA
AGGGAGCTGGGAAAAGGGGCTCGAAGTGTTAAACAGTACGCTTCCGGAATTTGGTGTTGATTCTAAATCACTCGTCTTAA
GGGACGGATCAGGAATCTCACATATTGATGCTGTATCCTCAGATCAACTTTCACAGCTGTTATATGACATTCAGGATCAG
AGTTGGTTCTCGGCTTATCTAAATTCTTTACCTGTTGCGGGGAATCCTGACAGAATGGTGGGCGGAACGCTGAGAAACCG
CATGAAAGGCACTCCTGCACAAGGAAAAGTAAGAGCAAAAACCGGATCGCTCAGTACGGTAAGCTCTCTATCGGGGTACG
CAGAAACAAAGAGCGGAAAAAAACTTGTTTTCTCTATTCTTCTAAATGGATTGATTGATGAAGAGGATGGAAAAGATATT
GAGGATCAAATCGCTGTCATTTTGGCAAATCAATAA

Upstream 100 bases:

>100_bases
AGTGCTGGAGCCGGGAGAAGAAACGACAGCTAAAAGCAGTATCACCATTGAACTGAATCATCAATAGCCAATGATGAAGC
GCAGAGAGGCGGGGAAATTA

Downstream 100 bases:

>100_bases
TAGCGGGAGACCTGTTTTCCAAGAGAAAACAGGTTTTTTTATGTCTGTAATAACGCTTTGTCGAACCTTCCAACAATTAG
AAGAATGATGCACAAAAGAA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 4a; PBP-4a

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKKSIKLYVAVLLLFVVASVPYMHQAALAAEKQDALSGQIDKILADHPALEGAMAGITVRSAETGAVLYEHSGDTRMRPA
SSLKLLTAAAALSVLGENYSFTTEVRTDGTLKGKKLNGNLYLKGKGDPTLLPSDFDKMAEILKHSGVKVIKGNLIGDDTW
HDDMRLSPDMPWSDEYTYYGAPISALTASPNEDYDAGTVIVEVTPNQKEGEEPAVSVSPKTDYITIKNDAKTTAAGSEKD
LTIEREHGTNTITIEGSVPVDANKTKEWISVWEPAGYALDLFKQSLKKQGITVKGDIKTGEAPSSSDVLLSHRSMPLSKL
FVPFMKLSNNGHAEVLVKEMGKVKKGEGSWEKGLEVLNSTLPEFGVDSKSLVLRDGSGISHIDAVSSDQLSQLLYDIQDQ
SWFSAYLNSLPVAGNPDRMVGGTLRNRMKGTPAQGKVRAKTGSLSTVSSLSGYAETKSGKKLVFSILLNGLIDEEDGKDI
EDQIAVILANQ

Sequences:

>Translated_491_residues
MKKSIKLYVAVLLLFVVASVPYMHQAALAAEKQDALSGQIDKILADHPALEGAMAGITVRSAETGAVLYEHSGDTRMRPA
SSLKLLTAAAALSVLGENYSFTTEVRTDGTLKGKKLNGNLYLKGKGDPTLLPSDFDKMAEILKHSGVKVIKGNLIGDDTW
HDDMRLSPDMPWSDEYTYYGAPISALTASPNEDYDAGTVIVEVTPNQKEGEEPAVSVSPKTDYITIKNDAKTTAAGSEKD
LTIEREHGTNTITIEGSVPVDANKTKEWISVWEPAGYALDLFKQSLKKQGITVKGDIKTGEAPSSSDVLLSHRSMPLSKL
FVPFMKLSNNGHAEVLVKEMGKVKKGEGSWEKGLEVLNSTLPEFGVDSKSLVLRDGSGISHIDAVSSDQLSQLLYDIQDQ
SWFSAYLNSLPVAGNPDRMVGGTLRNRMKGTPAQGKVRAKTGSLSTVSSLSGYAETKSGKKLVFSILLNGLIDEEDGKDI
EDQIAVILANQ
>Mature_491_residues
MKKSIKLYVAVLLLFVVASVPYMHQAALAAEKQDALSGQIDKILADHPALEGAMAGITVRSAETGAVLYEHSGDTRMRPA
SSLKLLTAAAALSVLGENYSFTTEVRTDGTLKGKKLNGNLYLKGKGDPTLLPSDFDKMAEILKHSGVKVIKGNLIGDDTW
HDDMRLSPDMPWSDEYTYYGAPISALTASPNEDYDAGTVIVEVTPNQKEGEEPAVSVSPKTDYITIKNDAKTTAAGSEKD
LTIEREHGTNTITIEGSVPVDANKTKEWISVWEPAGYALDLFKQSLKKQGITVKGDIKTGEAPSSSDVLLSHRSMPLSKL
FVPFMKLSNNGHAEVLVKEMGKVKKGEGSWEKGLEVLNSTLPEFGVDSKSLVLRDGSGISHIDAVSSDQLSQLLYDIQDQ
SWFSAYLNSLPVAGNPDRMVGGTLRNRMKGTPAQGKVRAKTGSLSTVSSLSGYAETKSGKKLVFSILLNGLIDEEDGKDI
EDQIAVILANQ

Specific function: Catalyzes DD-carboxypeptidase and transpeptidation reactions

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Secreted (Probable). Note=May be anchored in the membrane via a C-terminal amphiphatic alpha helix

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family

Homologues:

Organism=Escherichia coli, GI1789573, Length=442, Percent_Identity=28.0542986425339, Blast_Score=149, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DACC_BACSU (P39844)

Other databases:

- EMBL:   Z34883
- EMBL:   AL009126
- PIR:   I40455
- RefSeq:   NP_389717.1
- PDB:   1W5D
- PDB:   2J9P
- PDBsum:   1W5D
- PDBsum:   2J9P
- ProteinModelPortal:   P39844
- SMR:   P39844
- MEROPS:   S13.002
- EnsemblBacteria:   EBBACT00000000218
- GeneID:   939990
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU18350
- NMPDR:   fig|224308.1.peg.1839
- GenoList:   BSU18350
- GeneTree:   EBGT00050000002349
- HOGENOM:   HBG344715
- OMA:   MEARERP
- PhylomeDB:   P39844
- ProtClustDB:   CLSK881306
- BioCyc:   BSUB:BSU18350-MONOMER
- BRENDA:   3.4.16.4
- GO:   GO:0006508
- InterPro:   IPR012338
- InterPro:   IPR000667
- PRINTS:   PR00922
- TIGRFAMs:   TIGR00666

Pfam domain/function: PF02113 Peptidase_S13; SSF56601 PBP_transp_fold

EC number: =3.4.16.4

Molecular weight: Translated: 52892; Mature: 52892

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: ACT_SITE 81-81 ACT_SITE 84-84 ACT_SITE 328-328 BINDING 174-174 BINDING 381-381

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSIKLYVAVLLLFVVASVPYMHQAALAAEKQDALSGQIDKILADHPALEGAMAGITVR
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCEEEE
SAETGAVLYEHSGDTRMRPASSLKLLTAAAALSVLGENYSFTTEVRTDGTLKGKKLNGNL
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECEEECCEE
YLKGKGDPTLLPSDFDKMAEILKHSGVKVIKGNLIGDDTWHDDMRLSPDMPWSDEYTYYG
EEEECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEECCCCCCCCCCEEEC
APISALTASPNEDYDAGTVIVEVTPNQKEGEEPAVSVSPKTDYITIKNDAKTTAAGSEKD
CCHHEEECCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCC
LTIEREHGTNTITIEGSVPVDANKTKEWISVWEPAGYALDLFKQSLKKQGITVKGDIKTG
EEEEECCCCEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCC
EAPSSSDVLLSHRSMPLSKLFVPFMKLSNNGHAEVLVKEMGKVKKGEGSWEKGLEVLNST
CCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LPEFGVDSKSLVLRDGSGISHIDAVSSDQLSQLLYDIQDQSWFSAYLNSLPVAGNPDRMV
CHHCCCCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHH
GGTLRNRMKGTPAQGKVRAKTGSLSTVSSLSGYAETKSGKKLVFSILLNGLIDEEDGKDI
CHHHHHHCCCCCCCCEEEEECCCCHHHHHHCCHHHCCCCHHHHHHHHHHHHCCCCCCCCC
EDQIAVILANQ
CCEEEEEEECC
>Mature Secondary Structure
MKKSIKLYVAVLLLFVVASVPYMHQAALAAEKQDALSGQIDKILADHPALEGAMAGITVR
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCEEEE
SAETGAVLYEHSGDTRMRPASSLKLLTAAAALSVLGENYSFTTEVRTDGTLKGKKLNGNL
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECEEECCEE
YLKGKGDPTLLPSDFDKMAEILKHSGVKVIKGNLIGDDTWHDDMRLSPDMPWSDEYTYYG
EEEECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEECCCCCCCCCCEEEC
APISALTASPNEDYDAGTVIVEVTPNQKEGEEPAVSVSPKTDYITIKNDAKTTAAGSEKD
CCHHEEECCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCC
LTIEREHGTNTITIEGSVPVDANKTKEWISVWEPAGYALDLFKQSLKKQGITVKGDIKTG
EEEEECCCCEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCC
EAPSSSDVLLSHRSMPLSKLFVPFMKLSNNGHAEVLVKEMGKVKKGEGSWEKGLEVLNST
CCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LPEFGVDSKSLVLRDGSGISHIDAVSSDQLSQLLYDIQDQSWFSAYLNSLPVAGNPDRMV
CHHCCCCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHH
GGTLRNRMKGTPAQGKVRAKTGSLSTVSSLSGYAETKSGKKLVFSILLNGLIDEEDGKDI
CHHHHHHCCCCCCCCEEEEECCCCHHHHHHCCHHHCCCCHHHHHHHHHHHHCCCCCCCCC
EDQIAVILANQ
CCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7711903; 9384377