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The map label for this gene is glnA

Identifier: 16078809

GI number: 16078809

Start: 1878425

End: 1879759

Strand: Direct

Name: glnA

Synonym: BSU17460

Alternate gene names: 16078809

Gene position: 1878425-1879759 (Clockwise)

Preceding gene: 16078808

Following gene: 16078810

Centisome position: 44.56

GC content: 44.04

Gene sequence:

>1335_bases
ATGGCAAAGTACACTAGAGAAGATATCGAAAAATTAGTAAAAGAAGAAAACGTGAAGTATATCCGCCTTCAATTTACTGA
CATTCTTGGAACAATCAAGAATGTTGAGATTCCTGTAAGCCAGCTTGGAAAAGCGCTTGATAATAAAGTCATGTTTGACG
GTTCTTCTATTGAGGGATTCGTTCGTATCGAAGAGTCAGACATGTACCTGTATCCAGATCTAAATACATTTGTTATCTTC
CCATGGACAGCTGAAAAAGGTAAAGTAGCACGTTTCATCTGTGATATTTACAATCCGGATGGCACACCTTTTGAAGGTGA
CCCGCGAAACAACTTAAAACGGATTCTGAAAGAAATGGAAGACCTCGGCTTCAGTGATTTTAACCTTGGGCCTGAGCCTG
AATTCTTCTTATTCAAATTGGACGAAAAAGGCGAGCCGACGCTTGAACTAAACGACAAAGGCGGATATTTCGACTTAGCT
CCAACTGATTTAGGAGAAAACTGCCGCCGCGATATCGTACTTGAGCTTGAAGAGATGGGCTTTGAAATCGAAGCGTCTCA
CCACGAAGTAGCACCTGGTCAGCACGAAATCGACTTTAAATATGCTGGAGCAGTCCGCTCTTGTGATGACATCCAAACAT
TTAAACTAGTTGTCAAAACAATTGCCCGTAAACACGGCCTGCATGCGACATTTATGCCAAAACCATTGTTCGGTGTAAAC
GGTTCAGGTATGCACTGCAATCTATCACTCTTCAAAAATGGTGTTAACGCATTCTTTGACGAAAACGCAGATCTTCAGTT
AAGTGAAACAGCGAAGCACTTCATTGCAGGTATCGTGAAGCACGCAACAAGCTTTACAGCAGTAACAAACCCGACAGTAA
ACTCTTACAAACGTCTTGTTCCTGGCTATGAAGCACCTTGTTATGTAGCATGGAGCGCGCAAAACAGAAGCCCGCTTATC
CGTATCCCGGCTTCTCGCGGCATCAGCACACGTGTTGAAGTACGCAGTGTAGACCCAGCTGCAAACCCATACCTTGCACT
TAGCGTATTGCTTGCTGCAGGATTAGACGGAATCAAAAACAAACTGGAAGCGCCGGCTCCAATCGACCGCAACATCTATG
TGATGAGCAAAGAAGAGCGCATGGAAAACGGAATCGTTGACCTTCCAGCAACACTTGCGGAAGCACTAGAAGAATTCAAA
TCAAACGAAGTCATGGTCAAAGCGCTGGGCGAGCACCTATTCGAACACTTCATCGAAGCAAAAGAAATCGAATGGGATAT
GTTCCGCACGCAAGTACATCCTTGGGAACGCGAACAGTATATGTCTCAGTATTAA

Upstream 100 bases:

>100_bases
AATACATTCCGTCAAGGCGACATGTCCCGCTTCTTTCATTAATCCGTTAGCAACTGTTTTGCTATATACATTTACCTTTT
AAGAGGAGGAATTTTACCAA

Downstream 100 bases:

>100_bases
TATCTCAATCCCTTGGCACTAAAAGTGTCAGGGGATTTTTTATGTTAATAGGTAATAAATTATGGAGCGGCACTGCTAAA
AAGCAGGGTCACTTTTAGTC

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAKYTREDIEKLVKEENVKYIRLQFTDILGTIKNVEIPVSQLGKALDNKVMFDGSSIEGFVRIEESDMYLYPDLNTFVIF
PWTAEKGKVARFICDIYNPDGTPFEGDPRNNLKRILKEMEDLGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLA
PTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYAGAVRSCDDIQTFKLVVKTIARKHGLHATFMPKPLFGVN
GSGMHCNLSLFKNGVNAFFDENADLQLSETAKHFIAGIVKHATSFTAVTNPTVNSYKRLVPGYEAPCYVAWSAQNRSPLI
RIPASRGISTRVEVRSVDPAANPYLALSVLLAAGLDGIKNKLEAPAPIDRNIYVMSKEERMENGIVDLPATLAEALEEFK
SNEVMVKALGEHLFEHFIEAKEIEWDMFRTQVHPWEREQYMSQY

Sequences:

>Translated_444_residues
MAKYTREDIEKLVKEENVKYIRLQFTDILGTIKNVEIPVSQLGKALDNKVMFDGSSIEGFVRIEESDMYLYPDLNTFVIF
PWTAEKGKVARFICDIYNPDGTPFEGDPRNNLKRILKEMEDLGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLA
PTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYAGAVRSCDDIQTFKLVVKTIARKHGLHATFMPKPLFGVN
GSGMHCNLSLFKNGVNAFFDENADLQLSETAKHFIAGIVKHATSFTAVTNPTVNSYKRLVPGYEAPCYVAWSAQNRSPLI
RIPASRGISTRVEVRSVDPAANPYLALSVLLAAGLDGIKNKLEAPAPIDRNIYVMSKEERMENGIVDLPATLAEALEEFK
SNEVMVKALGEHLFEHFIEAKEIEWDMFRTQVHPWEREQYMSQY
>Mature_443_residues
AKYTREDIEKLVKEENVKYIRLQFTDILGTIKNVEIPVSQLGKALDNKVMFDGSSIEGFVRIEESDMYLYPDLNTFVIFP
WTAEKGKVARFICDIYNPDGTPFEGDPRNNLKRILKEMEDLGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLAP
TDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYAGAVRSCDDIQTFKLVVKTIARKHGLHATFMPKPLFGVNG
SGMHCNLSLFKNGVNAFFDENADLQLSETAKHFIAGIVKHATSFTAVTNPTVNSYKRLVPGYEAPCYVAWSAQNRSPLIR
IPASRGISTRVEVRSVDPAANPYLALSVLLAAGLDGIKNKLEAPAPIDRNIYVMSKEERMENGIVDLPATLAEALEEFKS
NEVMVKALGEHLFEHFIEAKEIEWDMFRTQVHPWEREQYMSQY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family

Homologues:

Organism=Homo sapiens, GI221307485, Length=436, Percent_Identity=26.3761467889908, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1790301, Length=473, Percent_Identity=42.2832980972516, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI87081870, Length=462, Percent_Identity=28.7878787878788, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): GLNA_BACSU (P12425)

Other databases:

- EMBL:   M22811
- EMBL:   D00854
- EMBL:   U66480
- EMBL:   AL009126
- PIR:   JT0392
- RefSeq:   NP_389628.1
- PDB:   2FWX
- PDBsum:   2FWX
- ProteinModelPortal:   P12425
- SMR:   P12425
- DIP:   DIP-29670N
- EnsemblBacteria:   EBBACT00000003985
- GeneID:   940020
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17460
- NMPDR:   fig|224308.1.peg.1750
- GenoList:   BSU17460
- GeneTree:   EBGT00050000002746
- HOGENOM:   HBG553755
- OMA:   AMFDGSS
- PhylomeDB:   P12425
- ProtClustDB:   CLSK887335
- BioCyc:   BSUB:BSU17460-MONOMER
- BRENDA:   6.3.1.2
- GO:   GO:0005737
- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637
- Gene3D:   G3DSA:3.30.590.10
- TIGRFAMs:   TIGR00653

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N; SSF54368 Gln_synt_beta

EC number: =6.3.1.2

Molecular weight: Translated: 50279; Mature: 50148

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00180 GLNA_1; PS00182 GLNA_ADENYLATION; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYTREDIEKLVKEENVKYIRLQFTDILGTIKNVEIPVSQLGKALDNKVMFDGSSIEGF
CCCCCHHHHHHHHHHCCCEEEEEEEHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCEE
VRIEESDMYLYPDLNTFVIFPWTAEKGKVARFICDIYNPDGTPFEGDPRNNLKRILKEME
EEEECCCEEEECCCCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
DLGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLAPTDLGENCRRDIVLELEEMG
HCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCEEECCCCCCCHHHHHHHEEEHHHCC
FEIEASHHEVAPGQHEIDFKYAGAVRSCDDIQTFKLVVKTIARKHGLHATFMPKPLFGVN
EEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
GSGMHCNLSLFKNGVNAFFDENADLQLSETAKHFIAGIVKHATSFTAVTNPTVNSYKRLV
CCCCEEEHHHHHCCHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHC
PGYEAPCYVAWSAQNRSPLIRIPASRGISTRVEVRSVDPAANPYLALSVLLAAGLDGIKN
CCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHH
KLEAPAPIDRNIYVMSKEERMENGIVDLPATLAEALEEFKSNEVMVKALGEHLFEHFIEA
HCCCCCCCCCCEEEEEHHHHHHCCCEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KEIEWDMFRTQVHPWEREQYMSQY
HHCHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure 
AKYTREDIEKLVKEENVKYIRLQFTDILGTIKNVEIPVSQLGKALDNKVMFDGSSIEGF
CCCCHHHHHHHHHHCCCEEEEEEEHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCEE
VRIEESDMYLYPDLNTFVIFPWTAEKGKVARFICDIYNPDGTPFEGDPRNNLKRILKEME
EEEECCCEEEECCCCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
DLGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLAPTDLGENCRRDIVLELEEMG
HCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCEEECCCCCCCHHHHHHHEEEHHHCC
FEIEASHHEVAPGQHEIDFKYAGAVRSCDDIQTFKLVVKTIARKHGLHATFMPKPLFGVN
EEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
GSGMHCNLSLFKNGVNAFFDENADLQLSETAKHFIAGIVKHATSFTAVTNPTVNSYKRLV
CCCCEEEHHHHHCCHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHC
PGYEAPCYVAWSAQNRSPLIRIPASRGISTRVEVRSVDPAANPYLALSVLLAAGLDGIKN
CCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHH
KLEAPAPIDRNIYVMSKEERMENGIVDLPATLAEALEEFKSNEVMVKALGEHLFEHFIEA
HCCCCCCCCCCEEEEEHHHHHHCCCEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KEIEWDMFRTQVHPWEREQYMSQY
HHCHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2906311; 2565853; 9384377; 11719184