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The map label for this gene is infB

Identifier: 16078726

GI number: 16078726

Start: 1734009

End: 1736159

Strand: Direct

Name: infB

Synonym: BSU16630

Alternate gene names: 16078726

Gene position: 1734009-1736159 (Clockwise)

Preceding gene: 16078725

Following gene: 16078727

Centisome position: 41.13

GC content: 42.91

Gene sequence:

>2151_bases
ATGGCTAAAATGAGAGTATACGAATATGCAAAAGCGTTAAATGTTTCAAGTAAGGAAATTTTGACCGCACTGAAGAACAT
GGATTTAGAAGTGAATAATCACATGGCCATGCTTGAAGAAAAGGCCATTAAAAAGCTAGATGCCAAATATAAAAAAGGCG
GCGCAGCTGCTAAATCTCAAAAGCCAGCAGAAACGAACAAAAACAAACAGCCACAAGGGGTTAATCAGCAATCAGCTGGA
AATCAACCAAATAAAATTCGAGACGGAAAGAAGAATGACGTGCAGAATAATCAATTTAACAAAAACAAGAAGAATAACAA
CAACAAAAAAAATAAACGCAACAACAACAATAATAAAAACCAACATCAGCAAAAGCCTGTAAAGCCGAAAAAAGAGCTTC
CTGAGAAAATTACATTCTCTGGCACTTTAACAGTTGGCGCACTTGCTGAAGAGCTTGGCAAAGAGCCTTCAGAACTCATT
AAAAAGCTGATGCTTCTTGGCGTAATGGCAACCATTAACCAAGAGCTTGATAAAGACACAATCGAACTCATTGCATCAGA
ATATGGTGTTGAAACAGAAGAGGTCATTGTGCTTGAAGAAACAGAGCTGGAAAAATACGAAGAGCCTGATAATGAAGAGG
ATCTTGAAATTCGTCCTCCTGTCGTGACAATCATGGGCCACGTTGACCATGGGAAAACAACGCTTCTTGACAGCATCCGT
AAAACAAAGGTTGTTGAAGGGGAAGCAGGCGGAATCACTCAGCATATCGGCGCTTATCAAATTGAAGAGAACGGCAAGAA
AATCACGTTCTTGGATACACCGGGCCACGCCGCATTTACAACAATGCGCGCACGCGGTGCAGAAGTAACTGATATTACGA
TTCTTGTCGTAGCTGCCGATGACGGTGTCATGCCGCAAACAGTTGAAGCGATTAACCATGCAAAAGCAGCAGAGGTTCCA
ATCATCGTTGCTGTGAATAAAATAGATAAAGAATCCGCAAACCCTGACCGTGTAATGCAAGAACTGACGGAATACGGACT
TGTCCCTGAAGCTTGGGGCGGAGAAACCATTTTTGTGCCGCTTTCCGCTTTAACTGGAAAAGGCATTGATGAGCTCGTTG
AAATGATTTTGCTTGTCAGTGAGGTAGAGGAACTGAAAGCGAATCCGAATCGTCAGGCAAAAGGAACGGTTATTGAAGCT
GAACTCGATAAAGGCAGAGGGTCAGTCGCGACGTTGCTCGTACAGACTGGAACACTGCATGTCGGTGATCCGATCGTAGT
CGGCAATACATTTGGCCGTGTCCGTGCAATGGTCAACGACATTGGCCGCCGTGTGAAAACTGCCGGCCCGTCAACTCCGG
TTGAAATTACCGGTTTGAATGATGTCCCTCAAGCGGGAGACCAATTCCTTGTCTTTAAGGATGAAAAAACAGCTCGTTCT
GTCGGTGAAGCCCGTGCTTCGAAACAGCTTGAAGAGCAGCGCAGCGATAAAGCGAAGCTCAGTCTTGATGATTTATTTGA
GCAAATTAAGCAAGGTGATGTAAAAGACATCAACCTCATCGTAAAAGCTGACGTTCAAGGATCTGCTGAAGCTTTAACGG
CTGCACTTCAAAAAATTGAAGTAGAAGGCGTTAAAGTGAAAATCATCCATACAGGCGTTGGTGCGATTACTGAATCAGAC
ATTATCTTGGCATCTGCTTCCAATGCAATTGTTATCGGGTTTAATGTGAGACCGGACGGAAATGCTAAGAGTACGGCTGA
AGCTGAAAATGTAGATATTCGACTTCACCGTATCATTTACAAAGTAATCGACGAGATTGAAGCTGCCATGAAAGGTATGC
TTGATCCTGAATATGAAGAAAAAGTAATTGGTCAAGTAGAAGTACGCCAAACATTCAAAGTATCTAAAATCGGTACAATT
GCCGGCGGATATGTTACTGAAGGAACCATTACGCGCGACAGCGGCCTCCGTTTAATTCGTGACGGCGTCGTCATCTTTGA
AGGCGAAGTAGATGTTCTGAAACGCTTTAAAGACGATGTGAAAGAAGTTTCACAAGGCTATGAATGTGGTATTACAATTA
AGAAATACAATGACATTCGTGAAGGTGACATCCTTGAAGCGTTTGTCATGCAAGAAATTGAAAGAACGTGA

Upstream 100 bases:

>100_bases
TCTATCGGTAAAGAAGCCCGTGTCGTTGTCGCCGTCACTGACCAAGGTTTTGCGAATAAGCTGATCAGCTTGCTCGATTA
ATATTTTTGGGGGTGAACGA

Downstream 100 bases:

>100_bases
TCGGATTTGCGGAGTGTGAATGCATCATTTATGATGCAGGATCGCTAAAAGAAAAGCGTGCCGTTCTGAAGCGGATTTTA
ACCAGGGTTCAAAACAAGTT

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 716; Mature: 715

Protein sequence:

>716_residues
MAKMRVYEYAKALNVSSKEILTALKNMDLEVNNHMAMLEEKAIKKLDAKYKKGGAAAKSQKPAETNKNKQPQGVNQQSAG
NQPNKIRDGKKNDVQNNQFNKNKKNNNNKKNKRNNNNNKNQHQQKPVKPKKELPEKITFSGTLTVGALAEELGKEPSELI
KKLMLLGVMATINQELDKDTIELIASEYGVETEEVIVLEETELEKYEEPDNEEDLEIRPPVVTIMGHVDHGKTTLLDSIR
KTKVVEGEAGGITQHIGAYQIEENGKKITFLDTPGHAAFTTMRARGAEVTDITILVVAADDGVMPQTVEAINHAKAAEVP
IIVAVNKIDKESANPDRVMQELTEYGLVPEAWGGETIFVPLSALTGKGIDELVEMILLVSEVEELKANPNRQAKGTVIEA
ELDKGRGSVATLLVQTGTLHVGDPIVVGNTFGRVRAMVNDIGRRVKTAGPSTPVEITGLNDVPQAGDQFLVFKDEKTARS
VGEARASKQLEEQRSDKAKLSLDDLFEQIKQGDVKDINLIVKADVQGSAEALTAALQKIEVEGVKVKIIHTGVGAITESD
IILASASNAIVIGFNVRPDGNAKSTAEAENVDIRLHRIIYKVIDEIEAAMKGMLDPEYEEKVIGQVEVRQTFKVSKIGTI
AGGYVTEGTITRDSGLRLIRDGVVIFEGEVDVLKRFKDDVKEVSQGYECGITIKKYNDIREGDILEAFVMQEIERT

Sequences:

>Translated_716_residues
MAKMRVYEYAKALNVSSKEILTALKNMDLEVNNHMAMLEEKAIKKLDAKYKKGGAAAKSQKPAETNKNKQPQGVNQQSAG
NQPNKIRDGKKNDVQNNQFNKNKKNNNNKKNKRNNNNNKNQHQQKPVKPKKELPEKITFSGTLTVGALAEELGKEPSELI
KKLMLLGVMATINQELDKDTIELIASEYGVETEEVIVLEETELEKYEEPDNEEDLEIRPPVVTIMGHVDHGKTTLLDSIR
KTKVVEGEAGGITQHIGAYQIEENGKKITFLDTPGHAAFTTMRARGAEVTDITILVVAADDGVMPQTVEAINHAKAAEVP
IIVAVNKIDKESANPDRVMQELTEYGLVPEAWGGETIFVPLSALTGKGIDELVEMILLVSEVEELKANPNRQAKGTVIEA
ELDKGRGSVATLLVQTGTLHVGDPIVVGNTFGRVRAMVNDIGRRVKTAGPSTPVEITGLNDVPQAGDQFLVFKDEKTARS
VGEARASKQLEEQRSDKAKLSLDDLFEQIKQGDVKDINLIVKADVQGSAEALTAALQKIEVEGVKVKIIHTGVGAITESD
IILASASNAIVIGFNVRPDGNAKSTAEAENVDIRLHRIIYKVIDEIEAAMKGMLDPEYEEKVIGQVEVRQTFKVSKIGTI
AGGYVTEGTITRDSGLRLIRDGVVIFEGEVDVLKRFKDDVKEVSQGYECGITIKKYNDIREGDILEAFVMQEIERT
>Mature_715_residues
AKMRVYEYAKALNVSSKEILTALKNMDLEVNNHMAMLEEKAIKKLDAKYKKGGAAAKSQKPAETNKNKQPQGVNQQSAGN
QPNKIRDGKKNDVQNNQFNKNKKNNNNKKNKRNNNNNKNQHQQKPVKPKKELPEKITFSGTLTVGALAEELGKEPSELIK
KLMLLGVMATINQELDKDTIELIASEYGVETEEVIVLEETELEKYEEPDNEEDLEIRPPVVTIMGHVDHGKTTLLDSIRK
TKVVEGEAGGITQHIGAYQIEENGKKITFLDTPGHAAFTTMRARGAEVTDITILVVAADDGVMPQTVEAINHAKAAEVPI
IVAVNKIDKESANPDRVMQELTEYGLVPEAWGGETIFVPLSALTGKGIDELVEMILLVSEVEELKANPNRQAKGTVIEAE
LDKGRGSVATLLVQTGTLHVGDPIVVGNTFGRVRAMVNDIGRRVKTAGPSTPVEITGLNDVPQAGDQFLVFKDEKTARSV
GEARASKQLEEQRSDKAKLSLDDLFEQIKQGDVKDINLIVKADVQGSAEALTAALQKIEVEGVKVKIIHTGVGAITESDI
ILASASNAIVIGFNVRPDGNAKSTAEAENVDIRLHRIIYKVIDEIEAAMKGMLDPEYEEKVIGQVEVRQTFKVSKIGTIA
GGYVTEGTITRDSGLRLIRDGVVIFEGEVDVLKRFKDDVKEVSQGYECGITIKKYNDIREGDILEAFVMQEIERT

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=543, Percent_Identity=41.0681399631676, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI53729337, Length=543, Percent_Identity=41.0681399631676, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI84043963, Length=462, Percent_Identity=28.7878787878788, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI157426893, Length=302, Percent_Identity=27.8145695364238, Blast_Score=84, Evalue=6e-16,
Organism=Homo sapiens, GI18390331, Length=142, Percent_Identity=31.6901408450704, Blast_Score=66, Evalue=1e-10,
Organism=Escherichia coli, GI1789559, Length=596, Percent_Identity=55.7046979865772, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=229, Percent_Identity=34.061135371179, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1788922, Length=234, Percent_Identity=27.3504273504274, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI48994988, Length=169, Percent_Identity=33.7278106508876, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1790412, Length=125, Percent_Identity=37.6, Blast_Score=64, Evalue=5e-11,
Organism=Escherichia coli, GI1789737, Length=125, Percent_Identity=37.6, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=549, Percent_Identity=36.4298724954463, Blast_Score=318, Evalue=8e-87,
Organism=Caenorhabditis elegans, GI212656558, Length=557, Percent_Identity=26.9299820466786, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=220, Percent_Identity=29.0909090909091, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=246, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25141371, Length=254, Percent_Identity=29.5275590551181, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324550, Length=616, Percent_Identity=38.6363636363636, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319282, Length=487, Percent_Identity=27.7207392197125, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324761, Length=251, Percent_Identity=31.8725099601594, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28572034, Length=610, Percent_Identity=37.5409836065574, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24656849, Length=490, Percent_Identity=26.9387755102041, Blast_Score=132, Evalue=1e-30,
Organism=Drosophila melanogaster, GI160714833, Length=118, Percent_Identity=56.7796610169492, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI78706572, Length=217, Percent_Identity=29.4930875576037, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922690, Length=229, Percent_Identity=28.3842794759825, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=225, Percent_Identity=32, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17864358, Length=225, Percent_Identity=32, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 78538; Mature: 78406

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRVYEYAKALNVSSKEILTALKNMDLEVNNHMAMLEEKAIKKLDAKYKKGGAAAKSQ
CCCCHHHHHHHHHCCCHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCC
KPAETNKNKQPQGVNQQSAGNQPNKIRDGKKNDVQNNQFNKNKKNNNNKKNKRNNNNNKN
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QHQQKPVKPKKELPEKITFSGTLTVGALAEELGKEPSELIKKLMLLGVMATINQELDKDT
HHHCCCCCCHHHCCCEEEECCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IELIASEYGVETEEVIVLEETELEKYEEPDNEEDLEIRPPVVTIMGHVDHGKTTLLDSIR
HHHHHHHHCCCCCEEEEEECCHHHHHCCCCCCCCCEECCCEEEEEECCCCCHHHHHHHHH
KTKVVEGEAGGITQHIGAYQIEENGKKITFLDTPGHAAFTTMRARGAEVTDITILVVAAD
HHEEECCCCCCCHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHCCCEEEEEEEEEEECC
DGVMPQTVEAINHAKAAEVPIIVAVNKIDKESANPDRVMQELTEYGLVPEAWGGETIFVP
CCCCHHHHHHHHCCHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEE
LSALTGKGIDELVEMILLVSEVEELKANPNRQAKGTVIEAELDKGRGSVATLLVQTGTLH
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEEEECCEEE
VGDPIVVGNTFGRVRAMVNDIGRRVKTAGPSTPVEITGLNDVPQAGDQFLVFKDEKTARS
CCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEEEEECCHHHHH
VGEARASKQLEEQRSDKAKLSLDDLFEQIKQGDVKDINLIVKADVQGSAEALTAALQKIE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHE
VEGVKVKIIHTGVGAITESDIILASASNAIVIGFNVRPDGNAKSTAEAENVDIRLHRIIY
ECCEEEEEEEECCCCEECCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
KVIDEIEAAMKGMLDPEYEEKVIGQVEVRQTFKVSKIGTIAGGYVTEGTITRDSGLRLIR
HHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHCCEECCCEEECCEEECCCCCEEEE
DGVVIFEGEVDVLKRFKDDVKEVSQGYECGITIKKYNDIREGDILEAFVMQEIERT
CCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKMRVYEYAKALNVSSKEILTALKNMDLEVNNHMAMLEEKAIKKLDAKYKKGGAAAKSQ
CCCHHHHHHHHHCCCHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCC
KPAETNKNKQPQGVNQQSAGNQPNKIRDGKKNDVQNNQFNKNKKNNNNKKNKRNNNNNKN
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QHQQKPVKPKKELPEKITFSGTLTVGALAEELGKEPSELIKKLMLLGVMATINQELDKDT
HHHCCCCCCHHHCCCEEEECCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IELIASEYGVETEEVIVLEETELEKYEEPDNEEDLEIRPPVVTIMGHVDHGKTTLLDSIR
HHHHHHHHCCCCCEEEEEECCHHHHHCCCCCCCCCEECCCEEEEEECCCCCHHHHHHHHH
KTKVVEGEAGGITQHIGAYQIEENGKKITFLDTPGHAAFTTMRARGAEVTDITILVVAAD
HHEEECCCCCCCHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHCCCEEEEEEEEEEECC
DGVMPQTVEAINHAKAAEVPIIVAVNKIDKESANPDRVMQELTEYGLVPEAWGGETIFVP
CCCCHHHHHHHHCCHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEE
LSALTGKGIDELVEMILLVSEVEELKANPNRQAKGTVIEAELDKGRGSVATLLVQTGTLH
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEEEECCEEE
VGDPIVVGNTFGRVRAMVNDIGRRVKTAGPSTPVEITGLNDVPQAGDQFLVFKDEKTARS
CCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEEEEECCHHHHH
VGEARASKQLEEQRSDKAKLSLDDLFEQIKQGDVKDINLIVKADVQGSAEALTAALQKIE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHE
VEGVKVKIIHTGVGAITESDIILASASNAIVIGFNVRPDGNAKSTAEAENVDIRLHRIIY
ECCEEEEEEEECCCCEECCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
KVIDEIEAAMKGMLDPEYEEKVIGQVEVRQTFKVSKIGTIAGGYVTEGTITRDSGLRLIR
HHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHCCEECCCEEECCEEECCCCCEEEE
DGVVIFEGEVDVLKRFKDDVKEVSQGYECGITIKKYNDIREGDILEAFVMQEIERT
CCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA