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The map label for this gene is flhF

Identifier: 16078703

GI number: 16078703

Start: 1709747

End: 1710847

Strand: Direct

Name: flhF

Synonym: BSU16400

Alternate gene names: 16078703

Gene position: 1709747-1710847 (Clockwise)

Preceding gene: 255767372

Following gene: 16078704

Centisome position: 40.56

GC content: 40.33

Gene sequence:

>1101_bases
ATGAAAATAAAAAAATTTACTGCTGCTTCAATGCAAGAAGCGGCACTTCTCATCAGAAAAGAGTTAGGTAATGAAGCGGT
CATATTAAATTCGAAAAAAATTAAAAAGCGAAAATGGTTTGGCCTCGTCAACAAGCCTGCTGTTGAAGTAATCGCCGTAT
TGGATCAGGACTTTCTTGAGAAAAAAACGCCTCAGAAGGCAGCTGAACCGAAACAAACATTAAAAACTCCGGTCAGCAGT
CCAAAAATAGAGGAGAGAACATATCCCCCTCAAATTCCGGCGCAACAGGAGCTAGGTGATTTTTCAGCATATCAATCTGT
ACTTCCTGAGCCGCTGCGAAAAGCAGAAAAGCTTTTGCAAGAAACAGGGATCAAAGAGTCTACGAAAACGAACACTCTGA
AAAAATTGCTTCGCTTCTCTGTTGAAGCAGGCGGCTTGACTGAAGAAAATGTTGTCGGCAAACTTCAGGAAATACTATGC
GATATGCTGCCCTCAGCAGACAAGTGGCAGGAGCCAATTCATTCAAAATATATCGTTCTCTTTGGTTCAACAGGGGCTGG
TAAAACAACTACACTGGCTAAACTCGCAGCCATATCTATGCTTGAAAAACATAAAAAAATCGCTTTTATTACAACAGATA
CGTATCGTATTGCAGCTGTTGAACAGTTGAAAACATACGCTGAACTGCTGCAGGCTCCTCTGGAAGTTTGCTATACAAAA
GAAGAATTTCAGCAGGCGAAGGAGCTATTTTCTGAGTATGATCATGTGTTTGTTGATACAGCTGGAAGAAACTTCAAAGA
CCCGCAATACATTGATGAACTGAAAGAAACGATTCCTTTTGAGAGCAGCATTCAGTCATTTCTTGTTTTATCCGCGACTG
CAAAATATGAAGATATGAAGCATATTGTCAAACGGTTTTCATCCGTCCCTGTCAATCAGTATATTTTTACGAAAATAGAT
GAAACGACTTCCTTAGGTTCTGTATTTAATATACTGGCAGAATCAAAAATAGGTGTCGGGTTTATGACTAACGGCCAGAA
TGTGCCTGAGGATATACAGACTGTATCTCCACTTGGTTTTGTGAGGATGCTGTGCAGATGA

Upstream 100 bases:

>100_bases
AGCAGCTACTGGAACGGTACTTCCCTGATCTTCCGGTACTCTCTTATAACGAACTTGAGGCGAATGTTGAGGTACAAAGC
ATTGGAGTGGTGGATATTTA

Downstream 100 bases:

>100_bases
ACAGATATGACCAAGCAGCAACTTTACGGGCGAAAATGGAAAAACGTGAGCGCGTTCTGCCAATGGTTTATTCACAAAAA
GCGAAGACACTTGCTGTCAT

Product: flagellar biosynthesis regulator FlhF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKIKKFTAASMQEAALLIRKELGNEAVILNSKKIKKRKWFGLVNKPAVEVIAVLDQDFLEKKTPQKAAEPKQTLKTPVSS
PKIEERTYPPQIPAQQELGDFSAYQSVLPEPLRKAEKLLQETGIKESTKTNTLKKLLRFSVEAGGLTEENVVGKLQEILC
DMLPSADKWQEPIHSKYIVLFGSTGAGKTTTLAKLAAISMLEKHKKIAFITTDTYRIAAVEQLKTYAELLQAPLEVCYTK
EEFQQAKELFSEYDHVFVDTAGRNFKDPQYIDELKETIPFESSIQSFLVLSATAKYEDMKHIVKRFSSVPVNQYIFTKID
ETTSLGSVFNILAESKIGVGFMTNGQNVPEDIQTVSPLGFVRMLCR

Sequences:

>Translated_366_residues
MKIKKFTAASMQEAALLIRKELGNEAVILNSKKIKKRKWFGLVNKPAVEVIAVLDQDFLEKKTPQKAAEPKQTLKTPVSS
PKIEERTYPPQIPAQQELGDFSAYQSVLPEPLRKAEKLLQETGIKESTKTNTLKKLLRFSVEAGGLTEENVVGKLQEILC
DMLPSADKWQEPIHSKYIVLFGSTGAGKTTTLAKLAAISMLEKHKKIAFITTDTYRIAAVEQLKTYAELLQAPLEVCYTK
EEFQQAKELFSEYDHVFVDTAGRNFKDPQYIDELKETIPFESSIQSFLVLSATAKYEDMKHIVKRFSSVPVNQYIFTKID
ETTSLGSVFNILAESKIGVGFMTNGQNVPEDIQTVSPLGFVRMLCR
>Mature_366_residues
MKIKKFTAASMQEAALLIRKELGNEAVILNSKKIKKRKWFGLVNKPAVEVIAVLDQDFLEKKTPQKAAEPKQTLKTPVSS
PKIEERTYPPQIPAQQELGDFSAYQSVLPEPLRKAEKLLQETGIKESTKTNTLKKLLRFSVEAGGLTEENVVGKLQEILC
DMLPSADKWQEPIHSKYIVLFGSTGAGKTTTLAKLAAISMLEKHKKIAFITTDTYRIAAVEQLKTYAELLQAPLEVCYTK
EEFQQAKELFSEYDHVFVDTAGRNFKDPQYIDELKETIPFESSIQSFLVLSATAKYEDMKHIVKRFSSVPVNQYIFTKID
ETTSLGSVFNILAESKIGVGFMTNGQNVPEDIQTVSPLGFVRMLCR

Specific function: Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum

COG id: COG1419

COG function: function code N; Flagellar GTP-binding protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding SRP family

Homologues:

Organism=Escherichia coli, GI1788963, Length=166, Percent_Identity=31.3253012048193, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789874, Length=235, Percent_Identity=25.1063829787234, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FLHF_BACSU (Q01960)

Other databases:

- EMBL:   X66445
- EMBL:   AL009126
- PIR:   S25277
- RefSeq:   NP_389522.1
- PDB:   2PX0
- PDB:   2PX3
- PDBsum:   2PX0
- PDBsum:   2PX3
- ProteinModelPortal:   Q01960
- SMR:   Q01960
- DIP:   DIP-46493N
- EnsemblBacteria:   EBBACT00000002479
- GeneID:   940021
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16400
- NMPDR:   fig|224308.1.peg.1643
- GenoList:   BSU16400
- GeneTree:   EBGT00050000001995
- HOGENOM:   HBG752478
- OMA:   KPISFIT
- ProtClustDB:   PRK05703
- BioCyc:   BSUB:BSU16400-MONOMER
- InterPro:   IPR000897
- InterPro:   IPR020006
- SMART:   SM00962
- TIGRFAMs:   TIGR03499

Pfam domain/function: PF00448 SRP54

EC number: NA

Molecular weight: Translated: 41150; Mature: 41150

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKKFTAASMQEAALLIRKELGNEAVILNSKKIKKRKWFGLVNKPAVEVIAVLDQDFLE
CCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KKTPQKAAEPKQTLKTPVSSPKIEERTYPPQIPAQQELGDFSAYQSVLPEPLRKAEKLLQ
HCCCHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
ETGIKESTKTNTLKKLLRFSVEAGGLTEENVVGKLQEILCDMLPSADKWQEPIHSKYIVL
HCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEE
FGSTGAGKTTTLAKLAAISMLEKHKKIAFITTDTYRIAAVEQLKTYAELLQAPLEVCYTK
EECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCH
EEFQQAKELFSEYDHVFVDTAGRNFKDPQYIDELKETIPFESSIQSFLVLSATAKYEDMK
HHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
HIVKRFSSVPVNQYIFTKIDETTSLGSVFNILAESKIGVGFMTNGQNVPEDIQTVSPLGF
HHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCHHHH
VRMLCR
HHHHHC
>Mature Secondary Structure
MKIKKFTAASMQEAALLIRKELGNEAVILNSKKIKKRKWFGLVNKPAVEVIAVLDQDFLE
CCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KKTPQKAAEPKQTLKTPVSSPKIEERTYPPQIPAQQELGDFSAYQSVLPEPLRKAEKLLQ
HCCCHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
ETGIKESTKTNTLKKLLRFSVEAGGLTEENVVGKLQEILCDMLPSADKWQEPIHSKYIVL
HCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEE
FGSTGAGKTTTLAKLAAISMLEKHKKIAFITTDTYRIAAVEQLKTYAELLQAPLEVCYTK
EECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCH
EEFQQAKELFSEYDHVFVDTAGRNFKDPQYIDELKETIPFESSIQSFLVLSATAKYEDMK
HHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
HIVKRFSSVPVNQYIFTKIDETTSLGSVFNILAESKIGVGFMTNGQNVPEDIQTVSPLGF
HHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCHHHH
VRMLCR
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447978; 9384377