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The map label for this gene is rnhB

Identifier: 16078669

GI number: 16078669

Start: 1677451

End: 1678218

Strand: Direct

Name: rnhB

Synonym: BSU16060

Alternate gene names: 16078669

Gene position: 1677451-1678218 (Clockwise)

Preceding gene: 16078668

Following gene: 16078670

Centisome position: 39.79

GC content: 45.31

Gene sequence:

>768_bases
GTGAATACATTAACCGTAAAGGACATTAAAGACCGTTTGCAGGAAGTGAAGGATGCACAAGACCCATTTATTGCCCAATG
CGAAAACGACCCGAGAAAAAGCGTTCAAACGCTTGTAGAGCAATGGCTTAAAAAGCAAGCGAAAGAAAAAGCGCTGAAAG
AACAATGGGTGAATATGACTTCCTATGAAAGGCTGGCAAGAAACAAAGGATTTCGCTTGATTGCAGGTGTTGACGAGGTC
GGCCGGGGGCCATTGGCAGGACCAGTTGTCGCCAGCGCAGTCATCCTTCCAGAGGAATGTGAAATACTTGGGCTGACAGA
CTCCAAAAAGCTTTCAGAGAAAAAACGCGAGGAATATTACGAGCTTATTATGAAGGAAGCACTGGCGGTCGGGATAGGAA
TTGTAGAAGCTACTGTGATTGATGAGATCAATATATATGAAGCTTCAAAAATGGCAATGGTGAAAGCGATACAGGATTTG
TCGGATACACCTGATTATTTGCTTGTTGACGCAATGACACTTCCGCTCGACACGGCTCAGGCGTCAATTATAAAAGGCGA
TGCCAAAAGCGTGTCTATTGCGGCAGGTGCGTGTATCGCAAAAGTGACGAGGGACCGAATGATGAGCGCTTATGCCGAAA
CGTATCCCATGTACGGCTTTGAAAAAAATAAAGGCTATGGGACAAAAGAACATTTGGAAGCTCTCGCTGCATACGGCCCA
ACTGAATTGCACCGCAAAACTTTCGCTCCTGTTCAATCTTTCAGATAA

Upstream 100 bases:

>100_bases
AGGCTGTCATTTGAACAGCCGACGATGTAAAGGCGCGCAGAAATGCGGGTCTTTATTTTTTCTTGGCTGAACCGATACAT
ACAGTAAGGGAGAAGAGAAA

Downstream 100 bases:

>100_bases
ATCACCATGGACAAGGAGGCACCTTTTTAAAATGAACATACGAAATGATGTGCAAAAGGCGCTGCAGCACCTTTTCGGGC
GTACAGCCGTCCCGCAGGAA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNTLTVKDIKDRLQEVKDAQDPFIAQCENDPRKSVQTLVEQWLKKQAKEKALKEQWVNMTSYERLARNKGFRLIAGVDEV
GRGPLAGPVVASAVILPEECEILGLTDSKKLSEKKREEYYELIMKEALAVGIGIVEATVIDEINIYEASKMAMVKAIQDL
SDTPDYLLVDAMTLPLDTAQASIIKGDAKSVSIAAGACIAKVTRDRMMSAYAETYPMYGFEKNKGYGTKEHLEALAAYGP
TELHRKTFAPVQSFR

Sequences:

>Translated_255_residues
MNTLTVKDIKDRLQEVKDAQDPFIAQCENDPRKSVQTLVEQWLKKQAKEKALKEQWVNMTSYERLARNKGFRLIAGVDEV
GRGPLAGPVVASAVILPEECEILGLTDSKKLSEKKREEYYELIMKEALAVGIGIVEATVIDEINIYEASKMAMVKAIQDL
SDTPDYLLVDAMTLPLDTAQASIIKGDAKSVSIAAGACIAKVTRDRMMSAYAETYPMYGFEKNKGYGTKEHLEALAAYGP
TELHRKTFAPVQSFR
>Mature_255_residues
MNTLTVKDIKDRLQEVKDAQDPFIAQCENDPRKSVQTLVEQWLKKQAKEKALKEQWVNMTSYERLARNKGFRLIAGVDEV
GRGPLAGPVVASAVILPEECEILGLTDSKKLSEKKREEYYELIMKEALAVGIGIVEATVIDEINIYEASKMAMVKAIQDL
SDTPDYLLVDAMTLPLDTAQASIIKGDAKSVSIAAGACIAKVTRDRMMSAYAETYPMYGFEKNKGYGTKEHLEALAAYGP
TELHRKTFAPVQSFR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=182, Percent_Identity=48.3516483516484, Blast_Score=167, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_BACSU (O31744)

Other databases:

- EMBL:   AL009126
- PIR:   C69693
- RefSeq:   NP_389488.1
- ProteinModelPortal:   O31744
- SMR:   O31744
- EnsemblBacteria:   EBBACT00000002382
- GeneID:   940133
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16060
- NMPDR:   fig|224308.1.peg.1608
- GenoList:   BSU16060
- GeneTree:   EBGT00050000002677
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   O31744
- ProtClustDB:   PRK00015
- BioCyc:   BSUB:BSU16060-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 28355; Mature: 28355

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLTVKDIKDRLQEVKDAQDPFIAQCENDPRKSVQTLVEQWLKKQAKEKALKEQWVNMT
CCCCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SYERLARNKGFRLIAGVDEVGRGPLAGPVVASAVILPEECEILGLTDSKKLSEKKREEYY
HHHHHHHCCCCEEEECCHHHCCCCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHH
ELIMKEALAVGIGIVEATVIDEINIYEASKMAMVKAIQDLSDTPDYLLVDAMTLPLDTAQ
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCHHH
ASIIKGDAKSVSIAAGACIAKVTRDRMMSAYAETYPMYGFEKNKGYGTKEHLEALAAYGP
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCC
TELHRKTFAPVQSFR
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTLTVKDIKDRLQEVKDAQDPFIAQCENDPRKSVQTLVEQWLKKQAKEKALKEQWVNMT
CCCCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SYERLARNKGFRLIAGVDEVGRGPLAGPVVASAVILPEECEILGLTDSKKLSEKKREEYY
HHHHHHHCCCCEEEECCHHHCCCCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHH
ELIMKEALAVGIGIVEATVIDEINIYEASKMAMVKAIQDLSDTPDYLLVDAMTLPLDTAQ
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCHHH
ASIIKGDAKSVSIAAGACIAKVTRDRMMSAYAETYPMYGFEKNKGYGTKEHLEALAAYGP
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCC
TELHRKTFAPVQSFR
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 10094689; 9888800