BacMap

Definition	Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession	NC_000964
Length	4,215,606

Click here to switch to the map view.

The map label for this gene is pyrAA

Identifier: 16078615

GI number: 16078615

Start: 1622657

End: 1623751

Strand: Direct

Name: pyrAA

Synonym: BSU15510

Alternate gene names: 16078615

Gene position: 1622657-1623751 (Clockwise)

Preceding gene: 255767363

Following gene: 16078616

Centisome position: 38.49

GC content: 44.93

Gene sequence:

>1095_bases
ATGAAGAGACGATTAGTACTGGAAAACGGAGCGGTATTCGAGGGAGAAGCTTTCGGAAGCTTAGAACACAACATGGGAGA
AGTCGTTTTTAATACTGGGATGACAGGCTATCAGGAAATTTTATCTGATCCTTCTTACTGCGGACAGATCGTAACATTAA
CATACCCGCTTATCGGAAATTACGGCATTAACCGTGATGATTTTGAATCCATTACCCCTTTTGTCAAAGGGCTGATCATC
AAAGAATTATGTGAGCTGCCTTCCAACTGGCGTTCAGCATACACCTTAGACGAGTATTTAAAAATGAAAAACATTCCCGG
ACTCCAGGGAATTGATACAAGGAAGCTGACAAGAATGATCCGCACGGCAGGCGCGCTAAAAGGAACATTCGCTTCATCTG
ATGAAGATATCGAAGCAGTGCTGAAAAGACTGAACGAAACGGAATTGCCAAGAAATCAAGTATCCCAAGTATCAGCAAAA
ACAGCATATCCGAGCCCGGGAAGAGGCAAACGCATTGTCTTGGTTGACTTCGGCATGAAACACGGGATTCTAAGAGAGCT
GAACAAACGGAAATGTGACGTCATCGTTGTGCCTTACAACATTACAGCGGAAGAGGTGCTTCAGCTGAAACCGGACGGTA
TCATGCTTTCTAACGGACCTGGAGACCCGAAGGATGTGCCTGAAGCGATTGAAATGATTAAAGGTGTTCTTGGAAAAGTG
CCATTATTCGGAATATGTCTCGGCCACCAATTATTCGCGCTGGCGTGCGGGGCGAATACTGAAAAAATGAAATTCGGCCA
CAGGGGCTCAAACCACCCGGTAAAAGAGCTGGCTACAGGAAAAGTTGCCTTAACATCTCAAAACCATGGATATACAGTTT
CGTCTATCAGTAAAACAGAACTGGAAGTGACGCATATCGCAATTAACGACGATACGATTGAAGGGCTGAAGCATAAAACA
TTGCCGGCATTTACGGTTCAATATCATCCCGAAGCCTCACCTGGTCCTGAGGATGCCAACCATCTATTTGACAGATTCAT
CGAAATGATCGAAACAACAGAGAAAGAAGGGGAAGCGGTATGCCAAAACGCGTAG

Upstream 100 bases:

>100_bases
AAGGAAAAAATACACCATTCAACGGCATCAGTTGCACCGGCTGGCCGGTCGCTACAATTGCGGCAGGGAAGCTTGCTTAT
GAAGAGGGGAGACTTGTCAA

Downstream 100 bases:

>100_bases
ACATTAACAAAATTTTAGTAATCGGATCTGGACCGATCATCATCGGCCAAGCAGCAGAATTTGACTATGCGGGAACACAA
GCCTGTCTTGCTTTGAAAGA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKRRLVLENGAVFEGEAFGSLEHNMGEVVFNTGMTGYQEILSDPSYCGQIVTLTYPLIGNYGINRDDFESITPFVKGLII
KELCELPSNWRSAYTLDEYLKMKNIPGLQGIDTRKLTRMIRTAGALKGTFASSDEDIEAVLKRLNETELPRNQVSQVSAK
TAYPSPGRGKRIVLVDFGMKHGILRELNKRKCDVIVVPYNITAEEVLQLKPDGIMLSNGPGDPKDVPEAIEMIKGVLGKV
PLFGICLGHQLFALACGANTEKMKFGHRGSNHPVKELATGKVALTSQNHGYTVSSISKTELEVTHIAINDDTIEGLKHKT
LPAFTVQYHPEASPGPEDANHLFDRFIEMIETTEKEGEAVCQNA

Sequences:

>Translated_364_residues
MKRRLVLENGAVFEGEAFGSLEHNMGEVVFNTGMTGYQEILSDPSYCGQIVTLTYPLIGNYGINRDDFESITPFVKGLII
KELCELPSNWRSAYTLDEYLKMKNIPGLQGIDTRKLTRMIRTAGALKGTFASSDEDIEAVLKRLNETELPRNQVSQVSAK
TAYPSPGRGKRIVLVDFGMKHGILRELNKRKCDVIVVPYNITAEEVLQLKPDGIMLSNGPGDPKDVPEAIEMIKGVLGKV
PLFGICLGHQLFALACGANTEKMKFGHRGSNHPVKELATGKVALTSQNHGYTVSSISKTELEVTHIAINDDTIEGLKHKT
LPAFTVQYHPEASPGPEDANHLFDRFIEMIETTEKEGEAVCQNA
>Mature_364_residues
MKRRLVLENGAVFEGEAFGSLEHNMGEVVFNTGMTGYQEILSDPSYCGQIVTLTYPLIGNYGINRDDFESITPFVKGLII
KELCELPSNWRSAYTLDEYLKMKNIPGLQGIDTRKLTRMIRTAGALKGTFASSDEDIEAVLKRLNETELPRNQVSQVSAK
TAYPSPGRGKRIVLVDFGMKHGILRELNKRKCDVIVVPYNITAEEVLQLKPDGIMLSNGPGDPKDVPEAIEMIKGVLGKV
PLFGICLGHQLFALACGANTEKMKFGHRGSNHPVKELATGKVALTSQNHGYTVSSISKTELEVTHIAINDDTIEGLKHKT
LPAFTVQYHPEASPGPEDANHLFDRFIEMIETTEKEGEAVCQNA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=363, Percent_Identity=42.9752066115703, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI21361331, Length=369, Percent_Identity=34.4173441734417, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI169790915, Length=369, Percent_Identity=34.4173441734417, Blast_Score=221, Evalue=1e-57,
Organism=Escherichia coli, GI1786215, Length=378, Percent_Identity=47.0899470899471, Blast_Score=311, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI193204318, Length=366, Percent_Identity=38.2513661202186, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324878, Length=379, Percent_Identity=37.467018469657, Blast_Score=252, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6322331, Length=390, Percent_Identity=38.2051282051282, Blast_Score=249, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24642586, Length=374, Percent_Identity=40.3743315508021, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI45555749, Length=377, Percent_Identity=40.053050397878, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_BACSU (P25993)

Other databases:

- EMBL:   M59757
- EMBL:   AL009126
- PIR:   E39845
- RefSeq:   NP_389434.1
- ProteinModelPortal:   P25993
- SMR:   P25993
- EnsemblBacteria:   EBBACT00000001564
- GeneID:   937368
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15510
- NMPDR:   fig|224308.1.peg.1553
- GenoList:   BSU15510
- GeneTree:   EBGT00070000032080
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- PhylomeDB:   P25993
- ProtClustDB:   PRK12564
- BioCyc:   BSUB:BSU15510-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 40120; Mature: 40120

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 246-246 ACT_SITE 329-329 ACT_SITE 331-331

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRLVLENGAVFEGEAFGSLEHNMGEVVFNTGMTGYQEILSDPSYCGQIVTLTYPLIGN
CCCEEEECCCCEECCCCCCCHHHCCCCEEEECCCHHHHHHHCCHHHCCEEEEEEEHHHCC
YGINRDDFESITPFVKGLIIKELCELPSNWRSAYTLDEYLKMKNIPGLQGIDTRKLTRMI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RTAGALKGTFASSDEDIEAVLKRLNETELPRNQVSQVSAKTAYPSPGRGKRIVLVDFGMK
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCH
HGILRELNKRKCDVIVVPYNITAEEVLQLKPDGIMLSNGPGDPKDVPEAIEMIKGVLGKV
HHHHHHHHCCCCCEEEECCCCCHHHHHEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
PLFGICLGHQLFALACGANTEKMKFGHRGSNHPVKELATGKVALTSQNHGYTVSSISKTE
CHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHCCCCEEEEECCCCEEEECCCCCE
LEVTHIAINDDTIEGLKHKTLPAFTVQYHPEASPGPEDANHLFDRFIEMIETTEKEGEAV
EEEEEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
CQNA
CCCC
>Mature Secondary Structure
MKRRLVLENGAVFEGEAFGSLEHNMGEVVFNTGMTGYQEILSDPSYCGQIVTLTYPLIGN
CCCEEEECCCCEECCCCCCCHHHCCCCEEEECCCHHHHHHHCCHHHCCEEEEEEEHHHCC
YGINRDDFESITPFVKGLIIKELCELPSNWRSAYTLDEYLKMKNIPGLQGIDTRKLTRMI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RTAGALKGTFASSDEDIEAVLKRLNETELPRNQVSQVSAKTAYPSPGRGKRIVLVDFGMK
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCH
HGILRELNKRKCDVIVVPYNITAEEVLQLKPDGIMLSNGPGDPKDVPEAIEMIKGVLGKV
HHHHHHHHCCCCCEEEECCCCCHHHHHEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
PLFGICLGHQLFALACGANTEKMKFGHRGSNHPVKELATGKVALTSQNHGYTVSSISKTE
CHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHCCCCEEEEECCCCEEEECCCCCE
LEVTHIAINDDTIEGLKHKTLPAFTVQYHPEASPGPEDANHLFDRFIEMIETTEKEGEAV
EEEEEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
CQNA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1709162; 9384377