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The map label for this gene is lspA

Identifier: 16078609

GI number: 16078609

Start: 1616744

End: 1617208

Strand: Direct

Name: lspA

Synonym: BSU15450

Alternate gene names: 16078609

Gene position: 1616744-1617208 (Clockwise)

Preceding gene: 255767359

Following gene: 255767360

Centisome position: 38.35

GC content: 41.29

Gene sequence:

>465_bases
GTGCTGTATTATATGATTGCACTACTTATTATTGCAGCCGATCAATTGACAAAATGGCTAGTTGTTAAGAACATGGAACT
TGGCCAAAGCATTCCGATCATTGATCAGGTATTCTATATCACCTCCCATCGCAATACGGGTGCTGCATGGGGGATATTAG
CGGGTCAAATGTGGTTTTTCTACCTCATAACAACCGCTGTTATTATTGGTATTGTTTATTACATACAGCGTTATACAAAG
GGACAAAGGCTTCTTGGCGTTGCCCTCGGACTTATGCTTGGCGGTGCCATCGGCAACTTTATCGATCGGGCTGTCAGACA
GGAAGTTGTGGATTTTATCCATGTTATTATTGTCAATTACAATTACCCGATATTTAACATCGCGGACTCTTCACTATGTG
TCGGCGTAATGCTTTTATTTATACAAATGCTGTTGGACAGCGGGAAAAAGAAAAAGGAGCAATAG

Upstream 100 bases:

>100_bases
GTGTCTGTCCGGTTAATATGCCTGATATTTCTTTACTCCAGTGCTTTTTATTGATAAAATGCATAATGGATTAAAGTAAC
CGTAAACTGGAGGAACGTTT

Downstream 100 bases:

>100_bases
CATGAATCAAATTGATATAACCGCTTCAGAAGAACAAAAAAGTGAGCGGATTGATAAATTTTTGGCATCGACTGAGAATG
ATTGGTCAAGAACCCAGGTT

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MLYYMIALLIIAADQLTKWLVVKNMELGQSIPIIDQVFYITSHRNTGAAWGILAGQMWFFYLITTAVIIGIVYYIQRYTK
GQRLLGVALGLMLGGAIGNFIDRAVRQEVVDFIHVIIVNYNYPIFNIADSSLCVGVMLLFIQMLLDSGKKKKEQ

Sequences:

>Translated_154_residues
MLYYMIALLIIAADQLTKWLVVKNMELGQSIPIIDQVFYITSHRNTGAAWGILAGQMWFFYLITTAVIIGIVYYIQRYTK
GQRLLGVALGLMLGGAIGNFIDRAVRQEVVDFIHVIIVNYNYPIFNIADSSLCVGVMLLFIQMLLDSGKKKKEQ
>Mature_154_residues
MLYYMIALLIIAADQLTKWLVVKNMELGQSIPIIDQVFYITSHRNTGAAWGILAGQMWFFYLITTAVIIGIVYYIQRYTK
GQRLLGVALGLMLGGAIGNFIDRAVRQEVVDFIHVIIVNYNYPIFNIADSSLCVGVMLLFIQMLLDSGKKKKEQ

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=155, Percent_Identity=32.9032258064516, Blast_Score=84, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_BACSU (Q45479)

Other databases:

- EMBL:   U48870
- EMBL:   AL009126
- EMBL:   U08116
- PIR:   G69653
- RefSeq:   NP_389428.1
- MEROPS:   A08.001
- EnsemblBacteria:   EBBACT00000002354
- GeneID:   938172
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15450
- NMPDR:   fig|224308.1.peg.1547
- GenoList:   BSU15450
- GeneTree:   EBGT00050000001848
- HOGENOM:   HBG724422
- OMA:   SYVVDMI
- PhylomeDB:   Q45479
- ProtClustDB:   PRK00376
- BioCyc:   BSUB:BSU15450-MONOMER
- BRENDA:   3.4.23.36
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 17427; Mature: 17427

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 102-102 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

HASH(0xef47de4)-; HASH(0xe9c99dc)-; HASH(0xee649bc)-; HASH(0xee523e0)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYYMIALLIIAADQLTKWLVVKNMELGQSIPIIDQVFYITSHRNTGAAWGILAGQMWFF
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
YLITTAVIIGIVYYIQRYTKGQRLLGVALGLMLGGAIGNFIDRAVRQEVVDFIHVIIVNY
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NYPIFNIADSSLCVGVMLLFIQMLLDSGKKKKEQ
CCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCH
>Mature Secondary Structure
MLYYMIALLIIAADQLTKWLVVKNMELGQSIPIIDQVFYITSHRNTGAAWGILAGQMWFF
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
YLITTAVIIGIVYYIQRYTKGQRLLGVALGLMLGGAIGNFIDRAVRQEVVDFIHVIIVNY
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NYPIFNIADSSLCVGVMLLFIQMLLDSGKKKKEQ
CCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9141696; 9384377; 10497172