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The map label for this gene is dapL

Identifier: 16078483

GI number: 16078483

Start: 1489753

End: 1490877

Strand: Direct

Name: dapL

Synonym: BSU14190

Alternate gene names: 16078483

Gene position: 1489753-1490877 (Clockwise)

Preceding gene: 255767332

Following gene: 16078484

Centisome position: 35.34

GC content: 44.89

Gene sequence:

>1125_bases
ATGAAGATAGAGGAGCTCATCGCAATTCGCAGAGATCTGCATCGTATACCGGAGCTTGGATTTCAGGAGTTCAAAACCCA
GCAGTATTTATTAAATGTCTTGGAACAATATCCGCAAGACAGAATTGAAATTGAGAAATGGCGAACAGGGCTTTTTGTAA
AAGTGAACGGGACGGCGCCGGAAAAAATGCTGGCATACAGAGCGGATATCGATGCGCTTTCTATAGAAGAGCAAACTGGT
CTTCCATTCGCATCAGAGCATCACGGCAACATGCACGCCTGCGGTCACGATTTGCATATGACAATTGCACTCGGCATTAT
TGATCATTTTGTTCACCACCCAGTCAAACATGATTTGCTTTTTCTGTTTCAGCCGGCAGAGGAAGGGCCTGGCGGTGCGG
AACCAATGCTTGAGAGCGATGTATTAAAAAAATGGCAGCCTGATTTCATCACTGCCCTTCATATTGCTCCAGAGCTTCCG
GTAGGCACCATTGCGACAAAAAGCGGCCTTCTATTTGCGAATACATCAGAGCTAGTCATCGATCTGGAAGGCAAAGGGGG
ACATGCGGCATATCCGCATTTGGCTGAGGATATGGTTGTAGCAGCAAGTACACTTGTCACCCAGCTGCAAACGATTATCT
CTAGAAACACAGATCCGCTAGACAGTGCTGTTATTACAGTTGGTACCATTACCGGAGGCTCGGCACAAAATATCATTGCA
GAAACGGCCCACCTGGAAGGCACGATCCGCACGCTTTCTGAAGAATCGATGAAACAAGTAAAGGAACGGATTGAAGATGT
AGTGAAAGGAATCGAAATCGGATTCCGCTGCAAAGGAAAAGTGACATATCCGTCTGTATATCACCAAGTTTACAATACGA
GCGGATTAACAGAAGAATTTATGTCTTTTGTTGCTGAACATCAACTGGCGACAGTAATTGAAGCAAAAGAAGCAATGACT
GGAGAGGATTTTGGCTATATGCTGAAAAAATATCCCGGATTCATGTTCTGGCTCGGCGCTGATTCTGAACATGGGCTTCA
TCATGCTAAGCTGAATCCCGATGAAAATGCGATAGAAACAGCGGTTCATGTCATGACAGGTTATTTTTCTGTTTATGCCA
ATTAA

Upstream 100 bases:

>100_bases
TGAAATCAAGCAGGAATTACGCCAGCTGTAAGGAAAAGTTTGATAAAAAAGGCGTGGATAATATATCCACGCCTTATTCG
TATAGGCAGGAGGAATAGAA

Downstream 100 bases:

>100_bases
GTACAAATGAAAGAATAAAATTCCATAAAATGAGCACTCTAGTAAACGGAGGTGTTCAAAAATGGCTAAAAAAATTGGAA
TTGAACAATCGTTATCAGAT

Product: N-acetyl-diaminopimelate deacetylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKIEELIAIRRDLHRIPELGFQEFKTQQYLLNVLEQYPQDRIEIEKWRTGLFVKVNGTAPEKMLAYRADIDALSIEEQTG
LPFASEHHGNMHACGHDLHMTIALGIIDHFVHHPVKHDLLFLFQPAEEGPGGAEPMLESDVLKKWQPDFITALHIAPELP
VGTIATKSGLLFANTSELVIDLEGKGGHAAYPHLAEDMVVAASTLVTQLQTIISRNTDPLDSAVITVGTITGGSAQNIIA
ETAHLEGTIRTLSEESMKQVKERIEDVVKGIEIGFRCKGKVTYPSVYHQVYNTSGLTEEFMSFVAEHQLATVIEAKEAMT
GEDFGYMLKKYPGFMFWLGADSEHGLHHAKLNPDENAIETAVHVMTGYFSVYAN

Sequences:

>Translated_374_residues
MKIEELIAIRRDLHRIPELGFQEFKTQQYLLNVLEQYPQDRIEIEKWRTGLFVKVNGTAPEKMLAYRADIDALSIEEQTG
LPFASEHHGNMHACGHDLHMTIALGIIDHFVHHPVKHDLLFLFQPAEEGPGGAEPMLESDVLKKWQPDFITALHIAPELP
VGTIATKSGLLFANTSELVIDLEGKGGHAAYPHLAEDMVVAASTLVTQLQTIISRNTDPLDSAVITVGTITGGSAQNIIA
ETAHLEGTIRTLSEESMKQVKERIEDVVKGIEIGFRCKGKVTYPSVYHQVYNTSGLTEEFMSFVAEHQLATVIEAKEAMT
GEDFGYMLKKYPGFMFWLGADSEHGLHHAKLNPDENAIETAVHVMTGYFSVYAN
>Mature_374_residues
MKIEELIAIRRDLHRIPELGFQEFKTQQYLLNVLEQYPQDRIEIEKWRTGLFVKVNGTAPEKMLAYRADIDALSIEEQTG
LPFASEHHGNMHACGHDLHMTIALGIIDHFVHHPVKHDLLFLFQPAEEGPGGAEPMLESDVLKKWQPDFITALHIAPELP
VGTIATKSGLLFANTSELVIDLEGKGGHAAYPHLAEDMVVAASTLVTQLQTIISRNTDPLDSAVITVGTITGGSAQNIIA
ETAHLEGTIRTLSEESMKQVKERIEDVVKGIEIGFRCKGKVTYPSVYHQVYNTSGLTEEFMSFVAEHQLATVIEAKEAMT
GEDFGYMLKKYPGFMFWLGADSEHGLHHAKLNPDENAIETAVHVMTGYFSVYAN

Specific function: Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate (Probable)

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapL subfamily

Homologues:

Organism=Escherichia coli, GI87081880, Length=313, Percent_Identity=27.1565495207668, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPL_BACSU (O34916)

Other databases:

- EMBL:   AJ222587
- EMBL:   AL009126
- PIR:   G69866
- RefSeq:   NP_389302.1
- HSSP:   P54970
- ProteinModelPortal:   O34916
- SMR:   O34916
- EnsemblBacteria:   EBBACT00000003073
- GeneID:   938805
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14190
- NMPDR:   fig|224308.1.peg.1421
- GenoList:   BSU14190
- GeneTree:   EBGT00070000031832
- HOGENOM:   HBG708500
- OMA:   IIGYRAD
- ProtClustDB:   CLSK2752387
- BioCyc:   BSUB:BSU14190-MONOMER
- HAMAP:   MF_01692
- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650
- PIRSF:   PIRSF005962
- TIGRFAMs:   TIGR01891

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.47

Molecular weight: Translated: 41558; Mature: 41558

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2; PS00178 AA_TRNA_LIGASE_I

Important sites: ACT_SITE 69-69 ACT_SITE 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEELIAIRRDLHRIPELGFQEFKTQQYLLNVLEQYPQDRIEIEKWRTGLFVKVNGTAP
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHEEHHHHCCEEEEEECCCCH
EKMLAYRADIDALSIEEQTGLPFASEHHGNMHACGHDLHMTIALGIIDHFVHHPVKHDLL
HHHHHHHCCCCCEEEHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCEE
FLFQPAEEGPGGAEPMLESDVLKKWQPDFITALHIAPELPVGTIATKSGLLFANTSELVI
EEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCEEEECCCEEEE
DLEGKGGHAAYPHLAEDMVVAASTLVTQLQTIISRNTDPLDSAVITVGTITGGSAQNIIA
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHH
ETAHLEGTIRTLSEESMKQVKERIEDVVKGIEIGFRCKGKVTYPSVYHQVYNTSGLTEEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHCCCCCHHHH
MSFVAEHQLATVIEAKEAMTGEDFGYMLKKYPGFMFWLGADSEHGLHHAKLNPDENAIET
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCHHHHHH
AVHVMTGYFSVYAN
HHHHHHHHHHEECC
>Mature Secondary Structure
MKIEELIAIRRDLHRIPELGFQEFKTQQYLLNVLEQYPQDRIEIEKWRTGLFVKVNGTAP
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHEEHHHHCCEEEEEECCCCH
EKMLAYRADIDALSIEEQTGLPFASEHHGNMHACGHDLHMTIALGIIDHFVHHPVKHDLL
HHHHHHHCCCCCEEEHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCEE
FLFQPAEEGPGGAEPMLESDVLKKWQPDFITALHIAPELPVGTIATKSGLLFANTSELVI
EEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCEEEECCCEEEE
DLEGKGGHAAYPHLAEDMVVAASTLVTQLQTIISRNTDPLDSAVITVGTITGGSAQNIIA
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHH
ETAHLEGTIRTLSEESMKQVKERIEDVVKGIEIGFRCKGKVTYPSVYHQVYNTSGLTEEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHCCCCCHHHH
MSFVAEHQLATVIEAKEAMTGEDFGYMLKKYPGFMFWLGADSEHGLHHAKLNPDENAIET
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCHHHHHH
AVHVMTGYFSVYAN
HHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377