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The map label for this gene is mtnU

Identifier: 16078421

GI number: 16078421

Start: 1424767

End: 1425546

Strand: Reverse

Name: mtnU

Synonym: BSU13570

Alternate gene names: 16078421

Gene position: 1425546-1424767 (Counterclockwise)

Preceding gene: 16078427

Following gene: 16078420

Centisome position: 33.82

GC content: 44.1

Gene sequence:

>780_bases
ATGAAATGGACGATATCTTGTCTTCAATTTGATATTTCATACGGAAAACCTTCGGAAAATATAAAAAAGGCTGAATTTTT
CATCGAAAAAGAAAGCAAACATGCTGATGTTCTTGTTCTGCCTGAATTATGGACGACCGGATATGATCTGGCCAATCTTG
ATGAACTCGCTGACGAAGACGGCCGCTCCGCTCAGAGCTGGCTGAAGAAAACGGCAAAAAAACATGGTGTTCATATTGTC
GCCGGATCGGTCGCTGTCAGGAAGAATTCTGATGTTTATAATACAATGTACATCGCGGATAAGGAAGGGCAAATCATTAA
AGAGTACAGAAAAGCCCATCTTTTTCAGCTGATGGATGAGCATCTGTATTTATCAGCCGGTTCCGAAGACGGATACTTTG
AGCTTGACGGCGTCAAAAGCTCTGGATTGATCTGTTACGATATCCGTTTTCCGGAATGGATCAGAAAACATACGACAAAA
GGCGCCAACGTGCTGTTTATTTCTGCGGAATGGCCTCTTCCCCGCCTTGATCATTGGAAAAGCCTGCTTATTGCGCGGGC
CATTGAAAACCAATGCTTTGTCGCAGCCTGCAATTGTACCGGTTCAAATCCGGATAATGAATTTGCAGGACATAGCCTGA
TTATTGATCCATGGGGACGTGTACTTGCTGAAGGCGGCCGGGAAGAAGGCATAGTCCGAGCGGAAATCGACCTTCAAGAA
AGCGCAGAAGTTCGGGAGAGCATTCCTGTTTTCGATGATATCAGAAAAGATTTATATTAA

Upstream 100 bases:

>100_bases
TTTCATCTATTCTCACCTTCTAAAAAAATCTTGAAATGATAGTCCATCATCATATATGGTTAAATGAAAAAAGTAAAGAA
AAATTTGGGGTGTTAAAAAG

Downstream 100 bases:

>100_bases
AAAATATATTGACAACTAACTAAATTACCTGTTACCATGTTCATCAACTGATAAATTAGAATTGTCAAACAAGTGAATAT
ATTCTCTTATCGAGAGTTGG

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKWTISCLQFDISYGKPSENIKKAEFFIEKESKHADVLVLPELWTTGYDLANLDELADEDGRSAQSWLKKTAKKHGVHIV
AGSVAVRKNSDVYNTMYIADKEGQIIKEYRKAHLFQLMDEHLYLSAGSEDGYFELDGVKSSGLICYDIRFPEWIRKHTTK
GANVLFISAEWPLPRLDHWKSLLIARAIENQCFVAACNCTGSNPDNEFAGHSLIIDPWGRVLAEGGREEGIVRAEIDLQE
SAEVRESIPVFDDIRKDLY

Sequences:

>Translated_259_residues
MKWTISCLQFDISYGKPSENIKKAEFFIEKESKHADVLVLPELWTTGYDLANLDELADEDGRSAQSWLKKTAKKHGVHIV
AGSVAVRKNSDVYNTMYIADKEGQIIKEYRKAHLFQLMDEHLYLSAGSEDGYFELDGVKSSGLICYDIRFPEWIRKHTTK
GANVLFISAEWPLPRLDHWKSLLIARAIENQCFVAACNCTGSNPDNEFAGHSLIIDPWGRVLAEGGREEGIVRAEIDLQE
SAEVRESIPVFDDIRKDLY
>Mature_259_residues
MKWTISCLQFDISYGKPSENIKKAEFFIEKESKHADVLVLPELWTTGYDLANLDELADEDGRSAQSWLKKTAKKHGVHIV
AGSVAVRKNSDVYNTMYIADKEGQIIKEYRKAHLFQLMDEHLYLSAGSEDGYFELDGVKSSGLICYDIRFPEWIRKHTTK
GANVLFISAEWPLPRLDHWKSLLIARAIENQCFVAACNCTGSNPDNEFAGHSLIIDPWGRVLAEGGREEGIVRAEIDLQE
SAEVRESIPVFDDIRKDLY

Specific function: May be involved in a regulatory step in the methylthioribose (MTR) recycling pathway

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain

Homologues:

Organism=Homo sapiens, GI9910460, Length=267, Percent_Identity=31.8352059925094, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI297632350, Length=258, Percent_Identity=29.4573643410853, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI5031947, Length=258, Percent_Identity=29.4573643410853, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI297632348, Length=258, Percent_Identity=29.4573643410853, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786412, Length=252, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17556280, Length=259, Percent_Identity=35.5212355212355, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323383, Length=280, Percent_Identity=28.5714285714286, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6322335, Length=287, Percent_Identity=27.8745644599303, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21355835, Length=254, Percent_Identity=32.6771653543307, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI17933642, Length=267, Percent_Identity=31.4606741573034, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNU_BACSU (O31664)

Other databases:

- EMBL:   AL009126
- PIR:   E69863
- RefSeq:   NP_389240.1
- HSSP:   O76463
- ProteinModelPortal:   O31664
- SMR:   O31664
- EnsemblBacteria:   EBBACT00000003066
- GeneID:   939328
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13570
- NMPDR:   fig|224308.1.peg.1359
- GenoList:   BSU13570
- GeneTree:   EBGT00050000001845
- HOGENOM:   HBG742418
- OMA:   EWPLVRL
- PhylomeDB:   O31664
- ProtClustDB:   CLSK2393381
- BioCyc:   BSUB:BSU13570-MONOMER
- InterPro:   IPR003010
- Gene3D:   G3DSA:3.60.110.10

Pfam domain/function: PF00795 CN_hydrolase; SSF56317 Ntlse/CNhydtse

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29400; Mature: 29400

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50263 CN_HYDROLASE; PS01227 UPF0012

Important sites: ACT_SITE 42-42 ACT_SITE 111-111 ACT_SITE 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWTISCLQFDISYGKPSENIKKAEFFIEKESKHADVLVLPELWTTGYDLANLDELADED
CCEEEEEEEEEECCCCCCCCHHHHHHEEECCCCCCCEEEECCHHCCCCCCCCHHHHHCCC
GRSAQSWLKKTAKKHGVHIVAGSVAVRKNSDVYNTMYIADKEGQIIKEYRKAHLFQLMDE
CCHHHHHHHHHHHHCCEEEEECCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
HLYLSAGSEDGYFELDGVKSSGLICYDIRFPEWIRKHTTKGANVLFISAEWPLPRLDHWK
HEEEEECCCCCEEEECCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
SLLIARAIENQCFVAACNCTGSNPDNEFAGHSLIIDPWGRVLAEGGREEGIVRAEIDLQE
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCEEEEEECCHH
SAEVRESIPVFDDIRKDLY
HHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MKWTISCLQFDISYGKPSENIKKAEFFIEKESKHADVLVLPELWTTGYDLANLDELADED
CCEEEEEEEEEECCCCCCCCHHHHHHEEECCCCCCCEEEECCHHCCCCCCCCHHHHHCCC
GRSAQSWLKKTAKKHGVHIVAGSVAVRKNSDVYNTMYIADKEGQIIKEYRKAHLFQLMDE
CCHHHHHHHHHHHHCCEEEEECCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
HLYLSAGSEDGYFELDGVKSSGLICYDIRFPEWIRKHTTKGANVLFISAEWPLPRLDHWK
HEEEEECCCCCEEEECCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
SLLIARAIENQCFVAACNCTGSNPDNEFAGHSLIIDPWGRVLAEGGREEGIVRAEIDLQE
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCEEEEEECCHH
SAEVRESIPVFDDIRKDLY
HHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921