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The map label for this gene is ppnK

Identifier: 16078226

GI number: 16078226

Start: 1237660

End: 1238460

Strand: Direct

Name: ppnK

Synonym: BSU11610

Alternate gene names: 16078226

Gene position: 1237660-1238460 (Clockwise)

Preceding gene: 16078225

Following gene: 16078227

Centisome position: 29.36

GC content: 45.57

Gene sequence:

>801_bases
ATGAAATTTGCCGTATCATCAAAAGGAGATCAAGTTTCTGATACGCTGAAAAGCAAAATACAGGCGTATTTATTGGATTT
TGATATGGAACTGGATGAAAATGAACCGGAAATCGTTATTTCAGTCGGAGGCGACGGAACGCTTTTGTATGCTTTTCACA
GATACAGCGACCGTTTGGACAAAACAGCATTTGTCGGCGTTCACACAGGTCATCTGGGTTTTTATGCCGACTGGGTTCCT
CATGAAATTGAAAAACTTGTTCTTGCCATCGCGAAAACGCCGTATCATACAGTCGAATATCCGCTTCTCGAAGTCATTGT
GACCTATCACGAAAACGAGCGGGAAGAAAGATACTTAGCTTTGAATGAATGTACGATTAAAAGCATCGAAGGAAGCCTTG
TTGCTGATGTGGAAATCAAAGGGCAGCTCTTTGAAACCTTCCGGGGCGACGGCCTCTGCCTGTCAACTCCATCAGGCAGC
ACAGCCTACAATAAGGCGCTGGGCGGAGCGATTATCCATCCGTCTATCAGGGCGATCCAGCTTGCGGAAATGGCTTCAAT
TAATAACCGCGTGTTCCGTACGGTCGGGTCACCTCTGCTTCTCCCATCCCATCATGATTGCATGATTAAGCCGAGAAACG
AAGTTGACTTTCAAGTGACGATTGACCATTTAACCCTTCTCCATAAGGATGTAAAGTCGATCCGCTGCCAAGTTGCATCC
GAAAAAGTGCGGTTTGCGAGATTCCGTCCGTTTCCATTTTGGAAAAGAGTCCAGGATTCGTTTATTGGAAAAGGTGAATA
G

Upstream 100 bases:

>100_bases
AGAGATGTCGGAGATCAGGGGTGAAGTACAGGAAGCTCAAGCTGCCTTTTCAAGAAAGAAAAAAGGAAGCGAGCAACAAT
AGGTAAAGGGGAAGAAGAGC

Downstream 100 bases:

>100_bases
AAAGAGGACATATCGTGGAGCATTTTTTTATCAGAAAGAACATTACTTCCGCTGAGGACGGGATGACTGTAAAAGAATAT
GCCGGTGAACTGGGAATTTC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 1

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKFAVSSKGDQVSDTLKSKIQAYLLDFDMELDENEPEIVISVGGDGTLLYAFHRYSDRLDKTAFVGVHTGHLGFYADWVP
HEIEKLVLAIAKTPYHTVEYPLLEVIVTYHENEREERYLALNECTIKSIEGSLVADVEIKGQLFETFRGDGLCLSTPSGS
TAYNKALGGAIIHPSIRAIQLAEMASINNRVFRTVGSPLLLPSHHDCMIKPRNEVDFQVTIDHLTLLHKDVKSIRCQVAS
EKVRFARFRPFPFWKRVQDSFIGKGE

Sequences:

>Translated_266_residues
MKFAVSSKGDQVSDTLKSKIQAYLLDFDMELDENEPEIVISVGGDGTLLYAFHRYSDRLDKTAFVGVHTGHLGFYADWVP
HEIEKLVLAIAKTPYHTVEYPLLEVIVTYHENEREERYLALNECTIKSIEGSLVADVEIKGQLFETFRGDGLCLSTPSGS
TAYNKALGGAIIHPSIRAIQLAEMASINNRVFRTVGSPLLLPSHHDCMIKPRNEVDFQVTIDHLTLLHKDVKSIRCQVAS
EKVRFARFRPFPFWKRVQDSFIGKGE
>Mature_266_residues
MKFAVSSKGDQVSDTLKSKIQAYLLDFDMELDENEPEIVISVGGDGTLLYAFHRYSDRLDKTAFVGVHTGHLGFYADWVP
HEIEKLVLAIAKTPYHTVEYPLLEVIVTYHENEREERYLALNECTIKSIEGSLVADVEIKGQLFETFRGDGLCLSTPSGS
TAYNKALGGAIIHPSIRAIQLAEMASINNRVFRTVGSPLLLPSHHDCMIKPRNEVDFQVTIDHLTLLHKDVKSIRCQVAS
EKVRFARFRPFPFWKRVQDSFIGKGE

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=152, Percent_Identity=32.8947368421053, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK1_BACSU (O31612)

Other databases:

- EMBL:   AL009126
- PIR:   F69844
- RefSeq:   NP_389043.1
- ProteinModelPortal:   O31612
- SMR:   O31612
- EnsemblBacteria:   EBBACT00000001007
- GeneID:   936414
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU11610
- NMPDR:   fig|224308.1.peg.1162
- GenoList:   BSU11610
- GeneTree:   EBGT00050000002705
- HOGENOM:   HBG314020
- OMA:   PSIRAIQ
- ProtClustDB:   PRK04885
- BioCyc:   BSUB:BSU11610-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 30013; Mature: 30013

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFAVSSKGDQVSDTLKSKIQAYLLDFDMELDENEPEIVISVGGDGTLLYAFHRYSDRLD
CCEECCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCEEEEEECCCCEEEEEEEHHHHHCC
KTAFVGVHTGHLGFYADWVPHEIEKLVLAIAKTPYHTVEYPLLEVIVTYHENEREERYLA
CEEEEEEEECCCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHEEE
LNECTIKSIEGSLVADVEIKGQLFETFRGDGLCLSTPSGSTAYNKALGGAIIHPSIRAIQ
ECCCEEEECCCCEEEEEEECHHHHHHHCCCCEEEECCCCCCHHHHHCCCEEECCCHHHHH
LAEMASINNRVFRTVGSPLLLPSHHDCMIKPRNEVDFQVTIDHLTLLHKDVKSIRCQVAS
HHHHHHHHHHHHHHCCCCEECCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
EKVRFARFRPFPFWKRVQDSFIGKGE
HHHHHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKFAVSSKGDQVSDTLKSKIQAYLLDFDMELDENEPEIVISVGGDGTLLYAFHRYSDRLD
CCEECCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCEEEEEECCCCEEEEEEEHHHHHCC
KTAFVGVHTGHLGFYADWVPHEIEKLVLAIAKTPYHTVEYPLLEVIVTYHENEREERYLA
CEEEEEEEECCCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHEEE
LNECTIKSIEGSLVADVEIKGQLFETFRGDGLCLSTPSGSTAYNKALGGAIIHPSIRAIQ
ECCCEEEECCCCEEEEEEECHHHHHHHCCCCEEEECCCCCCHHHHHCCCEEECCCHHHHH
LAEMASINNRVFRTVGSPLLLPSHHDCMIKPRNEVDFQVTIDHLTLLHKDVKSIRCQVAS
HHHHHHHHHHHHHHCCCCEECCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
EKVRFARFRPFPFWKRVQDSFIGKGE
HHHHHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377