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The map label for this gene is yjbJ

Identifier: 16078222

GI number: 16078222

Start: 1235163

End: 1235708

Strand: Reverse

Name: yjbJ

Synonym: BSU11570

Alternate gene names: 16078222

Gene position: 1235708-1235163 (Counterclockwise)

Preceding gene: 16078223

Following gene: 16078221

Centisome position: 29.31

GC content: 46.52

Gene sequence:

>546_bases
ATGCTGAACAGCGCGAATACAACAGCGCCGTCACTGCTTTCAGCGTATGGGCTTAACTCATATACGAGCAGCAATTCAGG
TTCAGTCACAAAAGCGGCAGAAAGCACTGAAACAGCTGTTGCAGATTCAGCTTCAAACAAACATGAAGCAAATCAGATCC
GTTCGGGAGACTTCTCTATTGACAGTGCGATTAAAAAAGCCGCTGATAAATATGGCGTTGATGAAAAACTGATCCGCGCC
GTCATCAAACAGGAATCAGGCTTTAATGCAAAAGCGGTCAGCGGAGCAGGTGCGATGGGCCTCATGCAGCTGATGCCGTC
AACAGCAAGCTCACTTGGCGTTTCCAATCCGCTTGATCCGCAGCAAAATGTGGAGGGCGGAACGAAATATTTAAAACAAA
TGCTGGATAAATACGACGGCAATGTGTCTATGGCATTAGCGGCTTACAACGCCGGACCGGGGAATGTTGACCGATACGGA
GGCATTCCTCCTTTCCAAGAAACGCAAAACTATGTAAAAAAAATAACTTCAGTTTATTACGCGTAA

Upstream 100 bases:

>100_bases
GGAAAGCCCGCTTTCAAGTTTCAGTTCTTTGCTCAGCCAATATACAAACGGTCTGACATCTCAGCAATCAGCACCTTCTT
CATCGCCAAGCCCGCTCAGC

Downstream 100 bases:

>100_bases
CCAGAGGTGCTCTTTCCCTCTGGTTTTTTTGCGAAGTGAAATCTTACGCATTCCTGATTTTTATGTGATATGATATGAAA
TACAAACCCCGGCTGTCAGC

Product: murein lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MLNSANTTAPSLLSAYGLNSYTSSNSGSVTKAAESTETAVADSASNKHEANQIRSGDFSIDSAIKKAADKYGVDEKLIRA
VIKQESGFNAKAVSGAGAMGLMQLMPSTASSLGVSNPLDPQQNVEGGTKYLKQMLDKYDGNVSMALAAYNAGPGNVDRYG
GIPPFQETQNYVKKITSVYYA

Sequences:

>Translated_181_residues
MLNSANTTAPSLLSAYGLNSYTSSNSGSVTKAAESTETAVADSASNKHEANQIRSGDFSIDSAIKKAADKYGVDEKLIRA
VIKQESGFNAKAVSGAGAMGLMQLMPSTASSLGVSNPLDPQQNVEGGTKYLKQMLDKYDGNVSMALAAYNAGPGNVDRYG
GIPPFQETQNYVKKITSVYYA
>Mature_181_residues
MLNSANTTAPSLLSAYGLNSYTSSNSGSVTKAAESTETAVADSASNKHEANQIRSGDFSIDSAIKKAADKYGVDEKLIRA
VIKQESGFNAKAVSGAGAMGLMQLMPSTASSLGVSNPLDPQQNVEGGTKYLKQMLDKYDGNVSMALAAYNAGPGNVDRYG
GIPPFQETQNYVKKITSVYYA

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI87082441, Length=119, Percent_Identity=38.655462184874, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI171474010, Length=148, Percent_Identity=30.4054054054054, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI87082191, Length=113, Percent_Identity=38.0530973451327, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YJBJ_BACSU (O31608)

Other databases:

- EMBL:   AL009126
- PIR:   B69844
- RefSeq:   NP_389039.1
- ProteinModelPortal:   O31608
- SMR:   O31608
- EnsemblBacteria:   EBBACT00000000523
- GeneID:   939382
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU11570
- NMPDR:   fig|224308.1.peg.1158
- GenoList:   BSU11570
- GeneTree:   EBGT00050000000244
- HOGENOM:   HBG712777
- OMA:   HEANQIR
- BioCyc:   BSUB:BSU11570-MONOMER
- InterPro:   IPR008258
- InterPro:   IPR000189

Pfam domain/function: PF01464 SLT

EC number: 3.2.1.- [C]

Molecular weight: Translated: 18993; Mature: 18993

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: ACT_SITE 85-85

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSANTTAPSLLSAYGLNSYTSSNSGSVTKAAESTETAVADSASNKHEANQIRSGDFSI
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHH
DSAIKKAADKYGVDEKLIRAVIKQESGFNAKAVSGAGAMGLMQLMPSTASSLGVSNPLDP
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC
QQNVEGGTKYLKQMLDKYDGNVSMALAAYNAGPGNVDRYGGIPPFQETQNYVKKITSVYY
HHCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
A
C
>Mature Secondary Structure
MLNSANTTAPSLLSAYGLNSYTSSNSGSVTKAAESTETAVADSASNKHEANQIRSGDFSI
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHH
DSAIKKAADKYGVDEKLIRAVIKQESGFNAKAVSGAGAMGLMQLMPSTASSLGVSNPLDP
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC
QQNVEGGTKYLKQMLDKYDGNVSMALAAYNAGPGNVDRYGGIPPFQETQNYVKKITSVYY
HHCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377