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The map label for this gene is argF

Identifier: 16078190

GI number: 16078190

Start: 1203461

End: 1204420

Strand: Direct

Name: argF

Synonym: BSU11250

Alternate gene names: 16078190

Gene position: 1203461-1204420 (Clockwise)

Preceding gene: 255767255

Following gene: 16078191

Centisome position: 28.55

GC content: 48.44

Gene sequence:

>960_bases
ATGCACACAGTGACGCAAACCAGTTTATACGGAAAAGATTTATTAACGTTAAAGGACCTTAGTGAAGAAGATATAAACGC
CTTGCTCGCAGAAGCCGGTGAGCTGAAACAAAACAAAATTCAGCCTATATTCCATGGCAAAACCTTGGCGATGATTTTTG
AAAAATCATCAACGCGGACCCGTGTTTCATTTGAAGCGGGCATGGCGCAGCTGGGCGGGAGCGCCCTCTTTCTAAGCCAG
AAAGATCTGCAGCTGGGGCGCGGTGAGACTGTCGCTGATACGGCAAAAGTGCTGTCAGGCTATGTGGATGCCATCATGAT
CCGGACCTTTGAACATGAAAAGGTGGAGGAACTTGCCAAAGAAGCTGACATTCCGGTGATCAATGGACTTACCGATAAAT
ACCACCCATGCCAGGCGCTGGCGGATCTCTTGACGATTAAGGAGATAAAAGGGAAGCTTAAAGGCGTAAAAGTCGCGTAC
ATCGGTGACGGAAATAACGTGGCGCACTCCTTGATGATCGGCTGCGCGAAAATGGGCTGTGATATCTCGATCGCTTCACC
AAAGGGATATGAAGTGTTAGATGAAGCAGCTGAAGCGGCAAAGACATATGCACTTCAATCCGGCTCTTCTGTCACGCTGA
CAGATGATCCGATTGAAGCTGTAAAAGACGCGGACGTGATTTATTCTGATGTGTTTACAAGCATGGGGCAGGAAGCGGAA
GAGCAAGAACGTCTTGCTGTCTTTGCGCCTTATCAGGTGAATGCGGCGCTGGTCAGCCATGCCAAGCCTGACTATACATT
TTTACATTGTCTCCCTGCGCATCGCGAGGAAGAAGTGACTGCGGAGATTATTGACGGGCCGAACTCTGCAGTGTTCCAGC
AGGCGGAAAACCGTCTTCATGTGCAAAAAGCACTGCTGAAGGCCATTTTATATAAAGGGGAATCATCAAAAAACTGCTGA

Upstream 100 bases:

>100_bases
TCTTTACAGAAGAAGAAACTGTGCGGGCGTTTTTACAAAGCGGTTCAGGCCATCCGCAGCCTGTATCTCTCAAAGACCTC
TATAAAAAGGAAGTGGCATC

Downstream 100 bases:

>100_bases
GCCAAACTCAGCAGTTTTTTTGATGGCAACATATTCACAGCCGCTTAGGCGCATACTACAGCAAAAAGGAAAAGGAGGAA
AAACAGTGGGACAGCAGCAC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MHTVTQTSLYGKDLLTLKDLSEEDINALLAEAGELKQNKIQPIFHGKTLAMIFEKSSTRTRVSFEAGMAQLGGSALFLSQ
KDLQLGRGETVADTAKVLSGYVDAIMIRTFEHEKVEELAKEADIPVINGLTDKYHPCQALADLLTIKEIKGKLKGVKVAY
IGDGNNVAHSLMIGCAKMGCDISIASPKGYEVLDEAAEAAKTYALQSGSSVTLTDDPIEAVKDADVIYSDVFTSMGQEAE
EQERLAVFAPYQVNAALVSHAKPDYTFLHCLPAHREEEVTAEIIDGPNSAVFQQAENRLHVQKALLKAILYKGESSKNC

Sequences:

>Translated_319_residues
MHTVTQTSLYGKDLLTLKDLSEEDINALLAEAGELKQNKIQPIFHGKTLAMIFEKSSTRTRVSFEAGMAQLGGSALFLSQ
KDLQLGRGETVADTAKVLSGYVDAIMIRTFEHEKVEELAKEADIPVINGLTDKYHPCQALADLLTIKEIKGKLKGVKVAY
IGDGNNVAHSLMIGCAKMGCDISIASPKGYEVLDEAAEAAKTYALQSGSSVTLTDDPIEAVKDADVIYSDVFTSMGQEAE
EQERLAVFAPYQVNAALVSHAKPDYTFLHCLPAHREEEVTAEIIDGPNSAVFQQAENRLHVQKALLKAILYKGESSKNC
>Mature_319_residues
MHTVTQTSLYGKDLLTLKDLSEEDINALLAEAGELKQNKIQPIFHGKTLAMIFEKSSTRTRVSFEAGMAQLGGSALFLSQ
KDLQLGRGETVADTAKVLSGYVDAIMIRTFEHEKVEELAKEADIPVINGLTDKYHPCQALADLLTIKEIKGKLKGVKVAY
IGDGNNVAHSLMIGCAKMGCDISIASPKGYEVLDEAAEAAKTYALQSGSSVTLTDDPIEAVKDADVIYSDVFTSMGQEAE
EQERLAVFAPYQVNAALVSHAKPDYTFLHCLPAHREEEVTAEIIDGPNSAVFQQAENRLHVQKALLKAILYKGESSKNC

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=310, Percent_Identity=44.8387096774194, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI18105007, Length=313, Percent_Identity=29.7124600638978, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI1790703, Length=335, Percent_Identity=38.5074626865672, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI1786469, Length=332, Percent_Identity=37.9518072289157, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI2367364, Length=318, Percent_Identity=34.5911949685535, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI48994908, Length=366, Percent_Identity=25.9562841530055, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193204318, Length=339, Percent_Identity=30.0884955752212, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6322373, Length=319, Percent_Identity=39.1849529780564, Blast_Score=236, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6322331, Length=315, Percent_Identity=30.4761904761905, Blast_Score=122, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24642586, Length=318, Percent_Identity=29.2452830188679, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_BACSU (P18186)

Other databases:

- EMBL:   X53360
- EMBL:   Z26919
- EMBL:   AL009126
- EMBL:   D86376
- PIR:   S11000
- RefSeq:   NP_389007.1
- ProteinModelPortal:   P18186
- SMR:   P18186
- EnsemblBacteria:   EBBACT00000002171
- GeneID:   936386
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU11250
- NMPDR:   fig|224308.1.peg.1126
- GenoList:   BSU11250
- GeneTree:   EBGT00050000002562
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- PhylomeDB:   P18186
- ProtClustDB:   PRK00779
- BioCyc:   BSUB:BSU11250-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 34664; Mature: 34664

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 108-108 BINDING 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTVTQTSLYGKDLLTLKDLSEEDINALLAEAGELKQNKIQPIFHGKTLAMIFEKSSTRT
CCCCCCCHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECCCCCE
RVSFEAGMAQLGGSALFLSQKDLQLGRGETVADTAKVLSGYVDAIMIRTFEHEKVEELAK
EEEHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
EADIPVINGLTDKYHPCQALADLLTIKEIKGKLKGVKVAYIGDGNNVAHSLMIGCAKMGC
HCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHCC
DISIASPKGYEVLDEAAEAAKTYALQSGSSVTLTDDPIEAVKDADVIYSDVFTSMGQEAE
CEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHH
EQERLAVFAPYQVNAALVSHAKPDYTFLHCLPAHREEEVTAEIIDGPNSAVFQQAENRLH
HHCCEEEECCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHH
VQKALLKAILYKGESSKNC
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHTVTQTSLYGKDLLTLKDLSEEDINALLAEAGELKQNKIQPIFHGKTLAMIFEKSSTRT
CCCCCCCHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECCCCCE
RVSFEAGMAQLGGSALFLSQKDLQLGRGETVADTAKVLSGYVDAIMIRTFEHEKVEELAK
EEEHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
EADIPVINGLTDKYHPCQALADLLTIKEIKGKLKGVKVAYIGDGNNVAHSLMIGCAKMGC
HCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHCC
DISIASPKGYEVLDEAAEAAKTYALQSGSSVTLTDDPIEAVKDADVIYSDVFTSMGQEAE
CEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHH
EQERLAVFAPYQVNAALVSHAKPDYTFLHCLPAHREEEVTAEIIDGPNSAVFQQAENRLH
HHCCEEEECCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHH
VQKALLKAILYKGESSKNC
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117745; 8025667; 9384377; 9335269