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The map label for this gene is yitJ

Identifier: 16078165

GI number: 16078165

Start: 1178757

End: 1180595

Strand: Reverse

Name: yitJ

Synonym: BSU11010

Alternate gene names: 16078165

Gene position: 1180595-1178757 (Counterclockwise)

Preceding gene: 16078166

Following gene: 16078164

Centisome position: 28.01

GC content: 48.29

Gene sequence:

>1839_bases
ATGGGACTTTTAGAAGATTTGCAAAGACAGGTGTTAATCGGTGACGGCGCCATGGGGACGCTCCTCTACTCCTATGGCAT
TGACAGGTGTTTTGAGGAGCTCAATATTTCAAAGCCGGAGGAAATTCAGCGCATACATAAAGCGTACGTTGAAGCGGGAG
CCAACATTATTCAAACGAATACGTACGGAGCCAACTATATTAAATTATCCAGACACGGGCTTGAGGATGACATCAAAAAA
ATGAATCAGGAGGCCGTAAAGATCGCGCGGGCTTCAGCAGGCGATGCATACGTGCTTGGAACGATGGGCGGCATACGCAC
ATTTAATAAAAACGCATACAGTCTTGACGAAATTAAGCGCAGCTTCCGCGAGCAGCTGTATCTGCTGCTGCATGAAGAGC
CGGATGGCTTGCTGTTAGAAACATATTATGATTTGGAAGAAGCCCGTGAAGTGCTGAAAATCGCGCGCAAAGAGACTGAT
CTTCCGATTATGCTCAATGTCTCCATGCACGAGCAAGGCGTATTGCAGGACGGAACGCCGCTTTCTGACGCATTGCGCTC
GATTGCGGATCTCGGAGCTGATATTGTCGGCATTAACTGCCGCCTCGGCCCTTATCACATGATTGAAGCGCTAAGCGAAG
TGCCGATTTTTGATGACGTATTCTTATCCGTTTATCCGAACAGCAGTCTTCCTTCGCTTGAAGAGGGACGGCTCGTCTAT
GAAACAGACGACACGTATTTTCAAAACAGCGCGTCAGAATTCCGTAAGCAAGGTGCGCGGATTATCGGCGGCTGCTGCGG
CACAACGCCGAATCACATCAGGGCGATGGCGGAAGCGGTTGGCGGGCTGGCTCCGATTACGGAAAAAGAAGTCAAAACCC
GGGCGAAAGAATTCATTTCTGTTCATCACGAGCGAACCGAGCCCGGCCTGGATGAGATCGCAGCGAAAAAACGCTCTATT
ATCGTAGAGCTGGACCCGCCGAAAAAACTAAGCTTTGACAAATTTCTGTCCGCTGCTGCTGAATTAAAAGAAGCGGGAAT
CGATGCCCTGACACTCGCCGACAATTCTTTGGCGACCCCGCGGATCAGCAATGTTGCCTGCGGCGCCCTTGTCAAGCAGC
AGCTCGATATGCGTTCCCTTGTCCATATTACGTGCCGTGACCGTAACATTATCGGCTTACAGTCACATTTAATGGGCCTT
GATACGTTAGGGCTGAATGACGTGCTTGCTATTACAGGTGACCCTTCTAAAATCGGGGATTTTCCTGGGGCGACGTCTGT
ATATGATTTAACATCTTTTGATTTAATCAGGCTGATCAAACAATTCAATGAAGGTCTGTCTCTGTCTGGAAAACCGCTCG
GCAAGAAAACGAATTTCTCAGTTGCCGCCGCATTTAATCCTAACGTCCGCCATCTGGATAAAGCGGTAAAACGGCTTGAG
AAAAAAATAGATTGCGGCGCAGATTATTTTGTTTCTCAGCCTGTCTATTCCGAGCAGCAGCTTGTCGATATCCATAACGA
AACAAAACATCTGAAGACGCCGATCTATATCGGCATCATGCCGCTGACAAGCAGCCGGAATGCCGAATTCATCCATAATG
AAATTCCCGGCATTAAGCTGTCTGATACGATTCGCGAAAAAATGGCCCATGCCGGTGAAGATAAAGAAAAGCAAAAGGCG
GAAGGCCTTGCAATTGCACGCTCCCTGCTGGATACGGCATGCGAGCTGTTTAACGGCATCTACTTGATAACGCCTTTCCT
CAGATCTGACTTAACCGCTGAGCTCACATCATATATTCAGCAAAAAGATGAGCAGCGCCAAAATATCTTTTTGCATTAA

Upstream 100 bases:

>100_bases
GAAGATAAGAAGAGACAAAATCACTGACAAAGTCTTCTTCTTAAGAGGACTTTTTTTATTTCTCTTTTTTCCTTGCTGAT
GTGAATAAAGGAGGCAGACA

Downstream 100 bases:

>100_bases
TTTCCAAAAGACTGCCTGATCGAATCGGGCAGTCTTTTTCAACGAGATTCGTGTTACGATATAGAAAAACAATCATGAGG
AGGAACATTATGCTCGAAGT

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MGLLEDLQRQVLIGDGAMGTLLYSYGIDRCFEELNISKPEEIQRIHKAYVEAGANIIQTNTYGANYIKLSRHGLEDDIKK
MNQEAVKIARASAGDAYVLGTMGGIRTFNKNAYSLDEIKRSFREQLYLLLHEEPDGLLLETYYDLEEAREVLKIARKETD
LPIMLNVSMHEQGVLQDGTPLSDALRSIADLGADIVGINCRLGPYHMIEALSEVPIFDDVFLSVYPNSSLPSLEEGRLVY
ETDDTYFQNSASEFRKQGARIIGGCCGTTPNHIRAMAEAVGGLAPITEKEVKTRAKEFISVHHERTEPGLDEIAAKKRSI
IVELDPPKKLSFDKFLSAAAELKEAGIDALTLADNSLATPRISNVACGALVKQQLDMRSLVHITCRDRNIIGLQSHLMGL
DTLGLNDVLAITGDPSKIGDFPGATSVYDLTSFDLIRLIKQFNEGLSLSGKPLGKKTNFSVAAAFNPNVRHLDKAVKRLE
KKIDCGADYFVSQPVYSEQQLVDIHNETKHLKTPIYIGIMPLTSSRNAEFIHNEIPGIKLSDTIREKMAHAGEDKEKQKA
EGLAIARSLLDTACELFNGIYLITPFLRSDLTAELTSYIQQKDEQRQNIFLH

Sequences:

>Translated_612_residues
MGLLEDLQRQVLIGDGAMGTLLYSYGIDRCFEELNISKPEEIQRIHKAYVEAGANIIQTNTYGANYIKLSRHGLEDDIKK
MNQEAVKIARASAGDAYVLGTMGGIRTFNKNAYSLDEIKRSFREQLYLLLHEEPDGLLLETYYDLEEAREVLKIARKETD
LPIMLNVSMHEQGVLQDGTPLSDALRSIADLGADIVGINCRLGPYHMIEALSEVPIFDDVFLSVYPNSSLPSLEEGRLVY
ETDDTYFQNSASEFRKQGARIIGGCCGTTPNHIRAMAEAVGGLAPITEKEVKTRAKEFISVHHERTEPGLDEIAAKKRSI
IVELDPPKKLSFDKFLSAAAELKEAGIDALTLADNSLATPRISNVACGALVKQQLDMRSLVHITCRDRNIIGLQSHLMGL
DTLGLNDVLAITGDPSKIGDFPGATSVYDLTSFDLIRLIKQFNEGLSLSGKPLGKKTNFSVAAAFNPNVRHLDKAVKRLE
KKIDCGADYFVSQPVYSEQQLVDIHNETKHLKTPIYIGIMPLTSSRNAEFIHNEIPGIKLSDTIREKMAHAGEDKEKQKA
EGLAIARSLLDTACELFNGIYLITPFLRSDLTAELTSYIQQKDEQRQNIFLH
>Mature_611_residues
GLLEDLQRQVLIGDGAMGTLLYSYGIDRCFEELNISKPEEIQRIHKAYVEAGANIIQTNTYGANYIKLSRHGLEDDIKKM
NQEAVKIARASAGDAYVLGTMGGIRTFNKNAYSLDEIKRSFREQLYLLLHEEPDGLLLETYYDLEEAREVLKIARKETDL
PIMLNVSMHEQGVLQDGTPLSDALRSIADLGADIVGINCRLGPYHMIEALSEVPIFDDVFLSVYPNSSLPSLEEGRLVYE
TDDTYFQNSASEFRKQGARIIGGCCGTTPNHIRAMAEAVGGLAPITEKEVKTRAKEFISVHHERTEPGLDEIAAKKRSII
VELDPPKKLSFDKFLSAAAELKEAGIDALTLADNSLATPRISNVACGALVKQQLDMRSLVHITCRDRNIIGLQSHLMGLD
TLGLNDVLAITGDPSKIGDFPGATSVYDLTSFDLIRLIKQFNEGLSLSGKPLGKKTNFSVAAAFNPNVRHLDKAVKRLEK
KIDCGADYFVSQPVYSEQQLVDIHNETKHLKTPIYIGIMPLTSSRNAEFIHNEIPGIKLSDTIREKMAHAGEDKEKQKAE
GLAIARSLLDTACELFNGIYLITPFLRSDLTAELTSYIQQKDEQRQNIFLH

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain

Homologues:

Organism=Homo sapiens, GI13162290, Length=320, Percent_Identity=32.1875, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI169790923, Length=321, Percent_Identity=26.791277258567, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI157266337, Length=327, Percent_Identity=28.7461773700306, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1790450, Length=326, Percent_Identity=30.0613496932515, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786456, Length=299, Percent_Identity=26.7558528428094, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17535405, Length=326, Percent_Identity=28.5276073619632, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324982, Length=316, Percent_Identity=25.3164556962025, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): YITJ_BACSU (O06745)

Other databases:

- EMBL:   Y09476
- EMBL:   AL009126
- PIR:   C69840
- RefSeq:   NP_388982.1
- HSSP:   O09171
- ProteinModelPortal:   O06745
- SMR:   O06745
- EnsemblBacteria:   EBBACT00000000026
- GeneID:   939796
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU11010
- NMPDR:   fig|224308.1.peg.1101
- GenoList:   BSU11010
- GeneTree:   EBGT00050000001526
- HOGENOM:   HBG293984
- OMA:   QSHLMGL
- PhylomeDB:   O06745
- ProtClustDB:   PRK08645
- BioCyc:   BSUB:BSU11010-MONOMER
- InterPro:   IPR003171
- InterPro:   IPR003726
- Gene3D:   G3DSA:3.20.20.330

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans; SSF82282 S_methyl_trans

EC number: =2.1.1.10; =1.5.1.20

Molecular weight: Translated: 67928; Mature: 67797

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLEDLQRQVLIGDGAMGTLLYSYGIDRCFEELNISKPEEIQRIHKAYVEAGANIIQTN
CCCHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEC
TYGANYIKLSRHGLEDDIKKMNQEAVKIARASAGDAYVLGTMGGIRTFNKNAYSLDEIKR
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHH
SFREQLYLLLHEEPDGLLLETYYDLEEAREVLKIARKETDLPIMLNVSMHEQGVLQDGTP
HHHHHHEEEEECCCCCEEEHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
LSDALRSIADLGADIVGINCRLGPYHMIEALSEVPIFDDVFLSVYPNSSLPSLEEGRLVY
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHEEECCCCCCCCCCCCEEEE
ETDDTYFQNSASEFRKQGARIIGGCCGTTPNHIRAMAEAVGGLAPITEKEVKTRAKEFIS
ECCCCHHHCCHHHHHHCCCCEEEHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VHHERTEPGLDEIAAKKRSIIVELDPPKKLSFDKFLSAAAELKEAGIDALTLADNSLATP
HHHHCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCC
RISNVACGALVKQQLDMRSLVHITCRDRNIIGLQSHLMGLDTLGLNDVLAITGDPSKIGD
CCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEHHHHHHCCCCCCCCCEEEEECCCHHCCC
FPGATSVYDLTSFDLIRLIKQFNEGLSLSGKPLGKKTNFSVAAAFNPNVRHLDKAVKRLE
CCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
KKIDCGADYFVSQPVYSEQQLVDIHNETKHLKTPIYIGIMPLTSSRNAEFIHNEIPGIKL
HHHCCCCCHHCCCCCCCCHHHHHHHCCHHHCCCCEEEEEEEECCCCCCHHHHHCCCCCEE
SDTIREKMAHAGEDKEKQKAEGLAIARSLLDTACELFNGIYLITPFLRSDLTAELTSYIQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHCCHHHHHHHHHH
QKDEQRQNIFLH
HHHHHHHCCCCC
>Mature Secondary Structure 
GLLEDLQRQVLIGDGAMGTLLYSYGIDRCFEELNISKPEEIQRIHKAYVEAGANIIQTN
CCHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEC
TYGANYIKLSRHGLEDDIKKMNQEAVKIARASAGDAYVLGTMGGIRTFNKNAYSLDEIKR
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHH
SFREQLYLLLHEEPDGLLLETYYDLEEAREVLKIARKETDLPIMLNVSMHEQGVLQDGTP
HHHHHHEEEEECCCCCEEEHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
LSDALRSIADLGADIVGINCRLGPYHMIEALSEVPIFDDVFLSVYPNSSLPSLEEGRLVY
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHEEECCCCCCCCCCCCEEEE
ETDDTYFQNSASEFRKQGARIIGGCCGTTPNHIRAMAEAVGGLAPITEKEVKTRAKEFIS
ECCCCHHHCCHHHHHHCCCCEEEHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VHHERTEPGLDEIAAKKRSIIVELDPPKKLSFDKFLSAAAELKEAGIDALTLADNSLATP
HHHHCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCC
RISNVACGALVKQQLDMRSLVHITCRDRNIIGLQSHLMGLDTLGLNDVLAITGDPSKIGD
CCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEHHHHHHCCCCCCCCCEEEEECCCHHCCC
FPGATSVYDLTSFDLIRLIKQFNEGLSLSGKPLGKKTNFSVAAAFNPNVRHLDKAVKRLE
CCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
KKIDCGADYFVSQPVYSEQQLVDIHNETKHLKTPIYIGIMPLTSSRNAEFIHNEIPGIKL
HHHCCCCCHHCCCCCCCCHHHHHHHCCHHHCCCCEEEEEEEECCCCCCHHHHHCCCCCEE
SDTIREKMAHAGEDKEKQKAEGLAIARSLLDTACELFNGIYLITPFLRSDLTAELTSYIQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHCCHHHHHHHHHH
QKDEQRQNIFLH
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377