Click here to switch to the map view.

The map label for this gene is yhfN

Identifier: 16078093

GI number: 16078093

Start: 1103104

End: 1104384

Strand: Direct

Name: yhfN

Synonym: BSU10290

Alternate gene names: 16078093

Gene position: 1103104-1104384 (Clockwise)

Preceding gene: 255767237

Following gene: 16078095

Centisome position: 26.17

GC content: 44.96

Gene sequence:

>1281_bases
ATGCGCAAGTGGATTGCGGCAGCAGGACTTGCTTACGTGCTGTACGGGCTGTTTTTTTATTGGTATTTTTTCCTGTCGGG
TGATTCCGCAATACCGGAAGCCGTGAAAGGGACGCAGGCTGATCCGGCTTCTTTCATGAAGCCGTCTGAGTTGGCAGTGG
CCGAGCAGTATTCGAATGTCAAGAATTTTTTATTTTTTATCGGGGTACCACTTGATTGGTTTCTGTTTTTTGTTCTGCTT
GTCAGCGGTGTTTCAAAGAAAATCAAGAAATGGATCGAAGCGGCCGTGCCTTTTCGGTTTTTGCAGACCGTTGGTTTTGT
GTTTGTGCTTTCGCTGATTACAACATTGGTGACGCTGCCTTTAGATTGGATAGGCTATCAAGTATCGCTTGACTATAACA
TTTCCACACAGACAACGGCCAGCTGGGCTAAGGATCAGGTTATCAGCTTTTGGATCAGCTTTCCAATCTTTACGCTTTGC
GTTCTCGTTTTTTATTGGCTGATCAAAAGGCATGAAAAAAAATGGTGGTTATACGCCTGGCTGTTAACAGTGCCGTTTTC
GCTGTTTCTGTTTTTTATTCAGCCGGTCATTATCGATCCTTTATACAATGATTTTTATCCGCTGAAAAACAAAGAGCTTG
AAAGCAAAATTTTAGAGCTGGCAGATGAAGCCAATATTCCGGCTGACCATGTATATGAAGTGAACATGTCAGAAAAAACA
AATGCGCTGAATGCCTATGTTACAGGAATTGGGGCCAATAAACGGATTGTATTGTGGGATACGACGCTGAACAAACTTGA
CGATTCAGAAATTCTGTTTATTATGGGCCACGAAATGGGCCATTATGTCATGAAGCACGTTTACATCGGTCTGGCTGGCT
ATTTGCTCGTGTCGCTCGCCGGATTTTATGTCATTGATAAGCTTTACAAGCGGACGGTTCGCCTAACCCGCAGCATGTTT
CATTTAGAAGGGCGGCATGATCTTGCGGCACTTCCGCTGTTATTGCTTTTGTTTTCTGTTTTGAGCTTTGCGGTTACGCC
TTTTTCTAATGCTGTCTCGCGTTATCAGGAGAATAAGGCTGACCAGTATGGGATCGAGTTGACAGAGAACAGAGAAGCCG
CTGTTAAAACGTTTCAGGATTTGGCCGTGACGGGGCTGAGCCAGGTTGATCCTCCGGTGCTTGTGAAGATTTTCAGAGGC
AGCCATCCGTCGATCATGGAACGGATTCAACATGCGGAGAAAGAAGAGAATGCGCCGGAACATCAGGATGCTGACAAATA
A

Upstream 100 bases:

>100_bases
TGCTTAAAAGGGTGGAAAAGTGCGGAACACAGCTTGGTTCTAAGAATTTGAATTTATGATTACAATAGAAGTAACGGGTT
GATGTGAGGAGTGAGGCGTT

Downstream 100 bases:

>100_bases
AAAGAAGCAGGTATGGAGGAACCTGCTTCTTTTTACTATTATTGTGCAGCTGCTTGTACGTTGATTAACCCTTTTCCATA
GTAGAAAGAGTTTCCAAGAT

Product: membrane metalloprotease

Products: NA

Alternate protein names: PSP23

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MRKWIAAAGLAYVLYGLFFYWYFFLSGDSAIPEAVKGTQADPASFMKPSELAVAEQYSNVKNFLFFIGVPLDWFLFFVLL
VSGVSKKIKKWIEAAVPFRFLQTVGFVFVLSLITTLVTLPLDWIGYQVSLDYNISTQTTASWAKDQVISFWISFPIFTLC
VLVFYWLIKRHEKKWWLYAWLLTVPFSLFLFFIQPVIIDPLYNDFYPLKNKELESKILELADEANIPADHVYEVNMSEKT
NALNAYVTGIGANKRIVLWDTTLNKLDDSEILFIMGHEMGHYVMKHVYIGLAGYLLVSLAGFYVIDKLYKRTVRLTRSMF
HLEGRHDLAALPLLLLLFSVLSFAVTPFSNAVSRYQENKADQYGIELTENREAAVKTFQDLAVTGLSQVDPPVLVKIFRG
SHPSIMERIQHAEKEENAPEHQDADK

Sequences:

>Translated_426_residues
MRKWIAAAGLAYVLYGLFFYWYFFLSGDSAIPEAVKGTQADPASFMKPSELAVAEQYSNVKNFLFFIGVPLDWFLFFVLL
VSGVSKKIKKWIEAAVPFRFLQTVGFVFVLSLITTLVTLPLDWIGYQVSLDYNISTQTTASWAKDQVISFWISFPIFTLC
VLVFYWLIKRHEKKWWLYAWLLTVPFSLFLFFIQPVIIDPLYNDFYPLKNKELESKILELADEANIPADHVYEVNMSEKT
NALNAYVTGIGANKRIVLWDTTLNKLDDSEILFIMGHEMGHYVMKHVYIGLAGYLLVSLAGFYVIDKLYKRTVRLTRSMF
HLEGRHDLAALPLLLLLFSVLSFAVTPFSNAVSRYQENKADQYGIELTENREAAVKTFQDLAVTGLSQVDPPVLVKIFRG
SHPSIMERIQHAEKEENAPEHQDADK
>Mature_426_residues
MRKWIAAAGLAYVLYGLFFYWYFFLSGDSAIPEAVKGTQADPASFMKPSELAVAEQYSNVKNFLFFIGVPLDWFLFFVLL
VSGVSKKIKKWIEAAVPFRFLQTVGFVFVLSLITTLVTLPLDWIGYQVSLDYNISTQTTASWAKDQVISFWISFPIFTLC
VLVFYWLIKRHEKKWWLYAWLLTVPFSLFLFFIQPVIIDPLYNDFYPLKNKELESKILELADEANIPADHVYEVNMSEKT
NALNAYVTGIGANKRIVLWDTTLNKLDDSEILFIMGHEMGHYVMKHVYIGLAGYLLVSLAGFYVIDKLYKRTVRLTRSMF
HLEGRHDLAALPLLLLLFSVLSFAVTPFSNAVSRYQENKADQYGIELTENREAAVKTFQDLAVTGLSQVDPPVLVKIFRG
SHPSIMERIQHAEKEENAPEHQDADK

Specific function: Unknown

COG id: COG0501

COG function: function code O; Zn-dependent protease with chaperone function

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M48B family

Homologues:

Organism=Homo sapiens, GI18379366, Length=438, Percent_Identity=24.8858447488584, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17505484, Length=341, Percent_Identity=25.5131964809384, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322577, Length=305, Percent_Identity=27.2131147540984, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922414, Length=334, Percent_Identity=23.6526946107784, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YHFN_BACSU (P40769)

Other databases:

- EMBL:   Y14083
- EMBL:   AL009126
- EMBL:   M22914
- PIR:   C69831
- RefSeq:   NP_388910.1
- ProteinModelPortal:   P40769
- MEROPS:   M48.009
- EnsemblBacteria:   EBBACT00000003696
- GeneID:   936314
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10290
- NMPDR:   fig|224308.1.peg.1029
- GenoList:   BSU10290
- GeneTree:   EBGT00050000002906
- HOGENOM:   HBG313760
- OMA:   NAYVTGI
- PhylomeDB:   P40769
- ProtClustDB:   CLSK887002
- BioCyc:   BSUB:BSU10290-MONOMER
- GO:   GO:0016020
- GO:   GO:0006508
- InterPro:   IPR001915

Pfam domain/function: PF01435 Peptidase_M48

EC number: NA

Molecular weight: Translated: 48875; Mature: 48875

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00142 ZINC_PROTEASE; PS00678 WD_REPEATS_1

Important sites: ACT_SITE 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKWIAAAGLAYVLYGLFFYWYFFLSGDSAIPEAVKGTQADPASFMKPSELAVAEQYSNV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHCCCCHHHHHHHHHHHH
KNFLFFIGVPLDWFLFFVLLVSGVSKKIKKWIEAAVPFRFLQTVGFVFVLSLITTLVTLP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDWIGYQVSLDYNISTQTTASWAKDQVISFWISFPIFTLCVLVFYWLIKRHEKKWWLYAW
HHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LLTVPFSLFLFFIQPVIIDPLYNDFYPLKNKELESKILELADEANIPADHVYEVNMSEKT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEECCCCCC
NALNAYVTGIGANKRIVLWDTTLNKLDDSEILFIMGHEMGHYVMKHVYIGLAGYLLVSLA
CHHHHEEEEECCCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
GFYVIDKLYKRTVRLTRSMFHLEGRHDLAALPLLLLLFSVLSFAVTPFSNAVSRYQENKA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
DQYGIELTENREAAVKTFQDLAVTGLSQVDPPVLVKIFRGSHPSIMERIQHAEKEENAPE
HHCCCEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
HQDADK
CCCCCC
>Mature Secondary Structure
MRKWIAAAGLAYVLYGLFFYWYFFLSGDSAIPEAVKGTQADPASFMKPSELAVAEQYSNV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHCCCCHHHHHHHHHHHH
KNFLFFIGVPLDWFLFFVLLVSGVSKKIKKWIEAAVPFRFLQTVGFVFVLSLITTLVTLP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDWIGYQVSLDYNISTQTTASWAKDQVISFWISFPIFTLCVLVFYWLIKRHEKKWWLYAW
HHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LLTVPFSLFLFFIQPVIIDPLYNDFYPLKNKELESKILELADEANIPADHVYEVNMSEKT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEECCCCCC
NALNAYVTGIGANKRIVLWDTTLNKLDDSEILFIMGHEMGHYVMKHVYIGLAGYLLVSLA
CHHHHEEEEECCCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
GFYVIDKLYKRTVRLTRSMFHLEGRHDLAALPLLLLLFSVLSFAVTPFSNAVSRYQENKA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
DQYGIELTENREAAVKTFQDLAVTGLSQVDPPVLVKIFRGSHPSIMERIQHAEKEENAPE
HHCCCEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
HQDADK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 3145906