BacMap

Definition	Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession	NC_000964
Length	4,215,606

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The map label for this gene is gltT

Identifier: 16078086

GI number: 16078086

Start: 1096560

End: 1097849

Strand: Reverse

Name: gltT

Synonym: BSU10220

Alternate gene names: 16078086

Gene position: 1097849-1096560 (Counterclockwise)

Preceding gene: 16078087

Following gene: 16078081

Centisome position: 26.04

GC content: 44.11

Gene sequence:

>1290_bases
ATGAAAAGAATTAAGTTTGGATTAGCCACACAAATATTCGTTGGACTTATTCTAGGTGTCATTGTTGGCGTCATTTGGTA
TGGCAACCCAGCATTGCCTACTTACCTGCAGCCAATCGGGGATCTCTTTTTACGCTTAATCAAAATGATAGTGATTCCTA
TTGTTGTCTCCAGCTTAATCATCGGTGTAGCCGGTGCAGGAAATGGAAAGCAAGTCGGTAAATTAGGCTTCAGAACGATT
CTGTACTTCGAGATCATCACGACTTTTGCCATTATTCTCGGACTAGCCCTTGCAAACATCTTCCATCCGGGTACAGGAGT
TAATATACACGAAGCTCAAAAATCGGACATCAGTCAATATGTTGAAACTGAAAAAGAACAAAGTAATAAATCAGTGGCGG
AAACGTTCCTCCATATTGTGCCGACAAACTTCTTCCAGTCATTGGTCGAAGGGGATCTTCTAGCCATCATCTGCTTTACA
GTACTATTTGCATTGGGTATTTCCGCGATCGGTGAAAGAGGCAAGCCTGTATTAGCCTTTTTTGAAGGTGTATCCCATGC
CATGTTCCACGTTGTAAACCTTGTGATGAAAGTGGCGCCATTCGGCGTTTTCGCTCTTATCGGAGTGACTGTGTCTAAAT
TCGGACTCGGTTCTCTTATCTCTCTCGGAAAGCTCGTCGGATTGGTTTATGTCGCGCTTGCTTTCTTCTTAATTGTTATT
TTCGGGATTGTCGCAAAAATTGCCGGCATCAGCATCTTCAAGTTCCTTGCTTACATGAAGGACGAAATCTTACTGGCGTT
CAGTACGTCCAGCTCTGAAACGGTTCTTCCGCGTATCATGGAAAAAATGGAGAAAATCGGATGTCCAAAAGGCATTGTTT
CATTTGTCATTCCGATCGGTTACACGTTTAACTTAGACGGTTCCGTTCTCTATCAATCTATTGCCGCACTCTTTTTAGCA
CAGGTATATGGCATTGATTTGACGATTTGGCACCAAATTACGCTTGTGCTCGTCTTAATGGTGACATCCAAAGGGATGGC
TGCCGTACCTGGTACTTCTTTTGTTGTCTTGCTTGCAACGCTTGGCACCATCGGCGTTCCGGCTGAAGGACTTGCCTTTA
TTGCCGGCGTAGACCGCATCATGGATATGGCACGTACAGTTGTCAACCTGACTGGTAACGCGCTTGCTGCCGTTGTGATG
TCTAAATGGGAAGGCATGTTTAATCCTGCGAAAGCAGAAACTGTTATGTCCCAATCGAAAACAGAACAAAACGCAACCAT
TTCTGGTTAA

Upstream 100 bases:

>100_bases
CGAATTGATTTCTTATCTATTATAATTTGATCTAATAGTGAGATTAAATATATGTCTATTCATGATATATGTTGATACTT
TGTTTTTTGGGAGGTAATCT

Downstream 100 bases:

>100_bases
TGAAAAGCCTGCGGGGTTCTTGCCCCGCAGGCTTTTTTAATCTTTAACAAGCACAAACAACTCTGTAATCAAAACAGTAT
CCTCATTCAGCTCCAGTTCA

Product: proton/sodium-glutamate symport protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKRIKFGLATQIFVGLILGVIVGVIWYGNPALPTYLQPIGDLFLRLIKMIVIPIVVSSLIIGVAGAGNGKQVGKLGFRTI
LYFEIITTFAIILGLALANIFHPGTGVNIHEAQKSDISQYVETEKEQSNKSVAETFLHIVPTNFFQSLVEGDLLAIICFT
VLFALGISAIGERGKPVLAFFEGVSHAMFHVVNLVMKVAPFGVFALIGVTVSKFGLGSLISLGKLVGLVYVALAFFLIVI
FGIVAKIAGISIFKFLAYMKDEILLAFSTSSSETVLPRIMEKMEKIGCPKGIVSFVIPIGYTFNLDGSVLYQSIAALFLA
QVYGIDLTIWHQITLVLVLMVTSKGMAAVPGTSFVVLLATLGTIGVPAEGLAFIAGVDRIMDMARTVVNLTGNALAAVVM
SKWEGMFNPAKAETVMSQSKTEQNATISG

Sequences:

>Translated_429_residues
MKRIKFGLATQIFVGLILGVIVGVIWYGNPALPTYLQPIGDLFLRLIKMIVIPIVVSSLIIGVAGAGNGKQVGKLGFRTI
LYFEIITTFAIILGLALANIFHPGTGVNIHEAQKSDISQYVETEKEQSNKSVAETFLHIVPTNFFQSLVEGDLLAIICFT
VLFALGISAIGERGKPVLAFFEGVSHAMFHVVNLVMKVAPFGVFALIGVTVSKFGLGSLISLGKLVGLVYVALAFFLIVI
FGIVAKIAGISIFKFLAYMKDEILLAFSTSSSETVLPRIMEKMEKIGCPKGIVSFVIPIGYTFNLDGSVLYQSIAALFLA
QVYGIDLTIWHQITLVLVLMVTSKGMAAVPGTSFVVLLATLGTIGVPAEGLAFIAGVDRIMDMARTVVNLTGNALAAVVM
SKWEGMFNPAKAETVMSQSKTEQNATISG
>Mature_429_residues
MKRIKFGLATQIFVGLILGVIVGVIWYGNPALPTYLQPIGDLFLRLIKMIVIPIVVSSLIIGVAGAGNGKQVGKLGFRTI
LYFEIITTFAIILGLALANIFHPGTGVNIHEAQKSDISQYVETEKEQSNKSVAETFLHIVPTNFFQSLVEGDLLAIICFT
VLFALGISAIGERGKPVLAFFEGVSHAMFHVVNLVMKVAPFGVFALIGVTVSKFGLGSLISLGKLVGLVYVALAFFLIVI
FGIVAKIAGISIFKFLAYMKDEILLAFSTSSSETVLPRIMEKMEKIGCPKGIVSFVIPIGYTFNLDGSVLYQSIAALFLA
QVYGIDLTIWHQITLVLVLMVTSKGMAAVPGTSFVVLLATLGTIGVPAEGLAFIAGVDRIMDMARTVVNLTGNALAAVVM
SKWEGMFNPAKAETVMSQSKTEQNATISG

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI40254478, Length=474, Percent_Identity=25.7383966244726, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI66773030, Length=443, Percent_Identity=28.2167042889391, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI169790839, Length=442, Percent_Identity=26.6968325791855, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI4827012, Length=434, Percent_Identity=24.6543778801843, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI194239697, Length=459, Percent_Identity=26.3616557734205, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI21314632, Length=430, Percent_Identity=27.6744186046512, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI5032093, Length=413, Percent_Identity=28.5714285714286, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI223468566, Length=288, Percent_Identity=29.1666666666667, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI223468564, Length=284, Percent_Identity=28.5211267605634, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI262359914, Length=391, Percent_Identity=26.3427109974425, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI301601644, Length=168, Percent_Identity=31.547619047619, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1790514, Length=420, Percent_Identity=54.2857142857143, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1789947, Length=424, Percent_Identity=37.2641509433962, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI1788024, Length=378, Percent_Identity=27.2486772486773, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1789473, Length=396, Percent_Identity=22.7272727272727, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=424, Percent_Identity=29.4811320754717, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71996953, Length=427, Percent_Identity=26.9320843091335, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71983099, Length=436, Percent_Identity=25.4587155963303, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71983106, Length=436, Percent_Identity=25.4587155963303, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI193206505, Length=436, Percent_Identity=27.5229357798165, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI193206654, Length=413, Percent_Identity=24.455205811138, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17541374, Length=406, Percent_Identity=24.384236453202, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24583025, Length=425, Percent_Identity=24.4705882352941, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17137668, Length=425, Percent_Identity=24.4705882352941, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24583023, Length=425, Percent_Identity=24.4705882352941, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281360481, Length=460, Percent_Identity=27.3913043478261, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281360483, Length=430, Percent_Identity=27.6744186046512, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17137666, Length=430, Percent_Identity=27.6744186046512, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLTT_BACSU (O07605)

Other databases:

- EMBL:   Y14083
- EMBL:   AL009126
- PIR:   D69635
- RefSeq:   NP_388903.1
- ProteinModelPortal:   O07605
- SMR:   O07605
- EnsemblBacteria:   EBBACT00000003596
- GeneID:   939770
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10220
- NMPDR:   fig|224308.1.peg.1022
- GenoList:   BSU10220
- GeneTree:   EBGT00070000031770
- HOGENOM:   HBG739804
- OMA:   MRIFKDE
- PhylomeDB:   O07605
- ProtClustDB:   CLSK886997
- BioCyc:   BSUB:BSU10220-MONOMER
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 45921; Mature: 45921

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd4074dc)-; HASH(0xd585194)-; HASH(0xde8ff6c)-; HASH(0xdb7d8bc)-; HASH(0xd770d04)-; HASH(0xc51c000)-; HASH(0xe141514)-; HASH(0xd4de8a0)-; HASH(0xbcbf48c)-; HASH(0xe124dc4)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCCCH
>Mature Secondary Structure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HHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377