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The map label for this gene is yhcK

Identifier: 16077977

GI number: 16077977

Start: 985734

End: 986813

Strand: Reverse

Name: yhcK

Synonym: BSU09120

Alternate gene names: 16077977

Gene position: 986813-985734 (Counterclockwise)

Preceding gene: 16077979

Following gene: 16077975

Centisome position: 23.41

GC content: 40.65

Gene sequence:

>1080_bases
TTGCTGAAAGAACTGTTTGTAAATTTGACCATTTTAATCACATTTAATTATCTCTTCACCCACCTGTTTAAAGAAAGGCT
AGTACATAAAAAAGACAGCATATCCTTTCAAGCTGTTAAAGGACTGGCCTGCGGTTTGCTCGGGGTTATTCTGATGGTTT
TTGGATTTACCTATCAGCATTCAATTATTGATCTTCGAAATATCCCTATTATGATTGCCGCTCTTTATGGAGGCTGGGTT
TCAACGGCAACGGCCCTGGCCATGATCACTGCAGGCCGCTTGCTGATTACGATGAACACATCTGCACTATACTCGGTTAT
TATCATTTGTATCGCTGCCATTCCGTCACTCATTGTTTCTAGAAGAAAAAAAGTTCAACTAAAACATGCTTTCTATCTTT
TAATAATCACAAATAGCTTGATCTCCTTTTCCTTTTATTTTCTTATAGATTTACATTCATACGAACTTCATTTGTATTTT
TGGATCATATCAATAGCGGGCGGCATGCTCAGTTTATATATTATTGATCATGAAACAAATGCACATCTTCTTTTTAAACA
ATATAAGTTTCAGGCGCACTTTGACTTTTTAACAGGCGTGTATAACCGAAGAAAATTTGAAGAAACCACAAAAGCTCTGT
ATCAGCAGGCGGCCGATACCCCGCATTTTCAATTTGCACTGATTTATATGGATATCGATCATTTTAAGACCATCAATGAC
CAATATGGCCATCATGAAGGAGATCAGGTGCTCAAAGAGCTAGGGTTACGTCTGAAGCAGACCATCCGAAATACTGATCC
TGCCGCTCGGATCGGCGGTGAAGAATTTGCCGTGCTCCTGCCGAACTGTTCTCTTGATAAAGCAGCCCGGATCGCTGAAC
GGATCAGGAGCACTGTAAGTGATGCACCGATTGTATTAACAAACGGCGACGAGCTTTCAGTTACGATATCACTAGGGGCC
GCTCATTACCCCAATAATACGGAACAGCCAGGATCCTTGCCCATTCTTGCCGATCAAATGCTTTACAAAGCGAAAGAAAC
GGGACGGAACAGAGTGTGTTTTTCAGAAAAAAAAGAATGA

Upstream 100 bases:

>100_bases
ATTTTAACTTATCAATTCAAGTAAAAAAACCTTATATAATGCCTTTTTTGTTCTTTTCTTTCCCTTCCATCTTTAGTACC
TTATATAAGGGTGATAATAT

Downstream 100 bases:

>100_bases
ATTCAATGTTCGAATTCATTCTTCCAATCTGAAAGACGTTTCAAAGGCAGAAAAGCCGGGACAAGATCTCCCTGATATTC
TTTTTCTATAAATGCCGCTG

Product: diguanylate cyclase or phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MLKELFVNLTILITFNYLFTHLFKERLVHKKDSISFQAVKGLACGLLGVILMVFGFTYQHSIIDLRNIPIMIAALYGGWV
STATALAMITAGRLLITMNTSALYSVIIICIAAIPSLIVSRRKKVQLKHAFYLLIITNSLISFSFYFLIDLHSYELHLYF
WIISIAGGMLSLYIIDHETNAHLLFKQYKFQAHFDFLTGVYNRRKFEETTKALYQQAADTPHFQFALIYMDIDHFKTIND
QYGHHEGDQVLKELGLRLKQTIRNTDPAARIGGEEFAVLLPNCSLDKAARIAERIRSTVSDAPIVLTNGDELSVTISLGA
AHYPNNTEQPGSLPILADQMLYKAKETGRNRVCFSEKKE

Sequences:

>Translated_359_residues
MLKELFVNLTILITFNYLFTHLFKERLVHKKDSISFQAVKGLACGLLGVILMVFGFTYQHSIIDLRNIPIMIAALYGGWV
STATALAMITAGRLLITMNTSALYSVIIICIAAIPSLIVSRRKKVQLKHAFYLLIITNSLISFSFYFLIDLHSYELHLYF
WIISIAGGMLSLYIIDHETNAHLLFKQYKFQAHFDFLTGVYNRRKFEETTKALYQQAADTPHFQFALIYMDIDHFKTIND
QYGHHEGDQVLKELGLRLKQTIRNTDPAARIGGEEFAVLLPNCSLDKAARIAERIRSTVSDAPIVLTNGDELSVTISLGA
AHYPNNTEQPGSLPILADQMLYKAKETGRNRVCFSEKKE
>Mature_359_residues
MLKELFVNLTILITFNYLFTHLFKERLVHKKDSISFQAVKGLACGLLGVILMVFGFTYQHSIIDLRNIPIMIAALYGGWV
STATALAMITAGRLLITMNTSALYSVIIICIAAIPSLIVSRRKKVQLKHAFYLLIITNSLISFSFYFLIDLHSYELHLYF
WIISIAGGMLSLYIIDHETNAHLLFKQYKFQAHFDFLTGVYNRRKFEETTKALYQQAADTPHFQFALIYMDIDHFKTIND
QYGHHEGDQVLKELGLRLKQTIRNTDPAARIGGEEFAVLLPNCSLDKAARIAERIRSTVSDAPIVLTNGDELSVTISLGA
AHYPNNTEQPGSLPILADQMLYKAKETGRNRVCFSEKKE

Specific function: Unknown

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain

Homologues:

Organism=Escherichia coli, GI87082007, Length=204, Percent_Identity=35.7843137254902, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=42.4242424242424, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788381, Length=209, Percent_Identity=33.9712918660287, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081881, Length=209, Percent_Identity=38.2775119617225, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=36.8421052631579, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI145693134, Length=172, Percent_Identity=34.8837209302326, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786584, Length=276, Percent_Identity=28.9855072463768, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=32.3529411764706, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=32.9479768786127, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=31.3253012048193, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787056, Length=165, Percent_Identity=32.1212121212121, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081974, Length=105, Percent_Identity=37.1428571428571, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788956, Length=153, Percent_Identity=32.6797385620915, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081977, Length=192, Percent_Identity=29.1666666666667, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YHCK_BACSU (P54595)

Other databases:

- EMBL:   X96983
- EMBL:   AL009126
- EMBL:   U58859
- PIR:   G69822
- RefSeq:   NP_388793.1
- ProteinModelPortal:   P54595
- SMR:   P54595
- EnsemblBacteria:   EBBACT00000002483
- GeneID:   936246
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09120
- NMPDR:   fig|224308.1.peg.912
- GenoList:   BSU09120
- GeneTree:   EBGT00070000031763
- HOGENOM:   HBG399362
- OMA:   MALRSIR
- ProtClustDB:   CLSK886936
- BioCyc:   BSUB:BSU09120-MONOMER
- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620
- SMART:   SM00267
- TIGRFAMs:   TIGR00254

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF; SSF55073 A/G_cyclase

EC number: NA

Molecular weight: Translated: 40693; Mature: 40693

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb3e672c)-; HASH(0xcf20694)-; HASH(0xde023fc)-; HASH(0xca6d6a4)-; HASH(0xde895a0)-; HASH(0xc8cc774)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKELFVNLTILITFNYLFTHLFKERLVHKKDSISFQAVKGLACGLLGVILMVFGFTYQH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
SIIDLRNIPIMIAALYGGWVSTATALAMITAGRLLITMNTSALYSVIIICIAAIPSLIVS
HHEECCCCCEEEEEHHCCHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHH
RRKKVQLKHAFYLLIITNSLISFSFYFLIDLHSYELHLYFWIISIAGGMLSLYIIDHETN
HHHHEEHHHHEEEEHHHHHHHHHEEEEEEEEECCEEEHHHHHHHHHCCEEEEEEEECCCC
AHLLFKQYKFQAHFDFLTGVYNRRKFEETTKALYQQAADTPHFQFALIYMDIDHFKTIND
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHCCH
QYGHHEGDQVLKELGLRLKQTIRNTDPAARIGGEEFAVLLPNCSLDKAARIAERIRSTVS
HCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHC
DAPIVLTNGDELSVTISLGAAHYPNNTEQPGSLPILADQMLYKAKETGRNRVCFSEKKE
CCCEEEECCCEEEEEEEECCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
>Mature Secondary Structure
MLKELFVNLTILITFNYLFTHLFKERLVHKKDSISFQAVKGLACGLLGVILMVFGFTYQH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
SIIDLRNIPIMIAALYGGWVSTATALAMITAGRLLITMNTSALYSVIIICIAAIPSLIVS
HHEECCCCCEEEEEHHCCHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHH
RRKKVQLKHAFYLLIITNSLISFSFYFLIDLHSYELHLYFWIISIAGGMLSLYIIDHETN
HHHHEEHHHHEEEEHHHHHHHHHEEEEEEEEECCEEEHHHHHHHHHCCEEEEEEEECCCC
AHLLFKQYKFQAHFDFLTGVYNRRKFEETTKALYQQAADTPHFQFALIYMDIDHFKTIND
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHCCH
QYGHHEGDQVLKELGLRLKQTIRNTDPAARIGGEEFAVLLPNCSLDKAARIAERIRSTVS
HCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHC
DAPIVLTNGDELSVTISLGAAHYPNNTEQPGSLPILADQMLYKAKETGRNRVCFSEKKE
CCCEEEECCCEEEEEEEECCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377