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The map label for this gene is yfhM

Identifier: 16077925

GI number: 16077925

Start: 929725

End: 930585

Strand: Direct

Name: yfhM

Synonym: BSU08590

Alternate gene names: 16077925

Gene position: 929725-930585 (Clockwise)

Preceding gene: 16077924

Following gene: 16077926

Centisome position: 22.05

GC content: 47.97

Gene sequence:

>861_bases
GTGGACGGAGTTAAATGCCAGTTTGTCAATACGAACGGAATCACGCTTCACGTTGCAGCCGCAGGACGGGAAGATGGCCC
GTTAATTGTCCTGCTCCATGGATTTCCTGAGTTTTGGTACGGCTGGAAAAATCAAATCAAACCGCTTGTTGATGCGGGTT
ACCGGGTCATTGCTCCTGATCAGCGGGGCTACAATCTCAGTGACAAACCGGAAGGAATTGATTCGTATCGGATTGATACA
TTAAGAGATGATATCATCGGGCTTATTACGCAATTCACTGATGAAAAAGCAATTGTCATCGGTCATGACTGGGGAGGAGC
TGTCGCATGGCATTTGGCTTCCACACGCCCGGAATATCTTGAAAAACTGATCGCGATCAATATCCCGCACCCGCACGTCA
TGAAAACCGTAACGCCGCTTTATCCGCCGCAATGGCTGAAAAGCTCGTATATCGCATACTTCCAGCTGCCCGATATACCG
GAGGCATCACTAAGGGAAAATGATTATGATACATTAGACAAAGCGATTGGATTATCCGACCGTCCCGCGCTTTTTACATC
CGAGGATGTCAGCAGGTACAAAGAAGCGTGGAAGCAGCCGGGCGCGCTGACAGCTATGCTGAACTGGTACAGAGCCCTCA
GAAAAGGAAGCTTGGCAGAGAAACCCTCTTACGAAACAGTACCTTACCGAATGATCTGGGGAATGGAAGACCGCTTTTTG
AGCAGAAAGCTTGCGAAAGAGACGGAAAGGCATTGCCCGAATGGACATCTCATCTTTGTTGATGAAGCTTCCCATTGGAT
TAACCACGAAAAGCCAGCCATCGTCAATCAGCTGATTTTGGAATATCTTAAGAACCAATAA

Upstream 100 bases:

>100_bases
ATTCTTCAGCTTATCCTGCTGATTGCCGCCTGTGTCATAACATTTATGCTAACGGAGAAAAGGCTGAAAATCATGACGCA
CAGTCAAGGAGGAGATAGAA

Downstream 100 bases:

>100_bases
GCTAAAATTTCTCTCCATCCGTCTGTCATAATGGCAGACTTTTTCTGTGCGTTTTTTTTCATAGTCTGTTTTAAACATGA
CAAAATAACATTAAAAATCA

Product: hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MDGVKCQFVNTNGITLHVAAAGREDGPLIVLLHGFPEFWYGWKNQIKPLVDAGYRVIAPDQRGYNLSDKPEGIDSYRIDT
LRDDIIGLITQFTDEKAIVIGHDWGGAVAWHLASTRPEYLEKLIAINIPHPHVMKTVTPLYPPQWLKSSYIAYFQLPDIP
EASLRENDYDTLDKAIGLSDRPALFTSEDVSRYKEAWKQPGALTAMLNWYRALRKGSLAEKPSYETVPYRMIWGMEDRFL
SRKLAKETERHCPNGHLIFVDEASHWINHEKPAIVNQLILEYLKNQ

Sequences:

>Translated_286_residues
MDGVKCQFVNTNGITLHVAAAGREDGPLIVLLHGFPEFWYGWKNQIKPLVDAGYRVIAPDQRGYNLSDKPEGIDSYRIDT
LRDDIIGLITQFTDEKAIVIGHDWGGAVAWHLASTRPEYLEKLIAINIPHPHVMKTVTPLYPPQWLKSSYIAYFQLPDIP
EASLRENDYDTLDKAIGLSDRPALFTSEDVSRYKEAWKQPGALTAMLNWYRALRKGSLAEKPSYETVPYRMIWGMEDRFL
SRKLAKETERHCPNGHLIFVDEASHWINHEKPAIVNQLILEYLKNQ
>Mature_286_residues
MDGVKCQFVNTNGITLHVAAAGREDGPLIVLLHGFPEFWYGWKNQIKPLVDAGYRVIAPDQRGYNLSDKPEGIDSYRIDT
LRDDIIGLITQFTDEKAIVIGHDWGGAVAWHLASTRPEYLEKLIAINIPHPHVMKTVTPLYPPQWLKSSYIAYFQLPDIP
EASLRENDYDTLDKAIGLSDRPALFTSEDVSRYKEAWKQPGALTAMLNWYRALRKGSLAEKPSYETVPYRMIWGMEDRFL
SRKLAKETERHCPNGHLIFVDEASHWINHEKPAIVNQLILEYLKNQ

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family

Homologues:

Organism=Homo sapiens, GI218777837, Length=291, Percent_Identity=38.1443298969072, Blast_Score=207, Evalue=7e-54,
Organism=Homo sapiens, GI218777840, Length=281, Percent_Identity=31.3167259786477, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI13376164, Length=281, Percent_Identity=31.3167259786477, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI27597073, Length=304, Percent_Identity=28.9473684210526, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI145611434, Length=270, Percent_Identity=24.4444444444444, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17554012, Length=289, Percent_Identity=35.2941176470588, Blast_Score=188, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17562298, Length=282, Percent_Identity=32.9787234042553, Blast_Score=171, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFHM_BACSU (O31581)

Other databases:

- EMBL:   D85082
- EMBL:   AL009126
- PIR:   F69801
- RefSeq:   NP_388739.1
- HSSP:   P34914
- ProteinModelPortal:   O31581
- SMR:   O31581
- EnsemblBacteria:   EBBACT00000002240
- GeneID:   936187
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08590
- NMPDR:   fig|224308.1.peg.858
- GenoList:   BSU08590
- GeneTree:   EBGT00050000000451
- HOGENOM:   HBG748003
- OMA:   PRHIVED
- ProtClustDB:   CLSK886911
- BioCyc:   BSUB:BSU08590-MONOMER
- InterPro:   IPR000073
- InterPro:   IPR000639
- PRINTS:   PR00111
- PRINTS:   PR00412

Pfam domain/function: PF00561 Abhydrolase_1

EC number: 3.7.1.- [C]

Molecular weight: Translated: 32759; Mature: 32759

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 103-103 ACT_SITE 210-210 ACT_SITE 265-265

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGVKCQFVNTNGITLHVAAAGREDGPLIVLLHGFPEFWYGWKNQIKPLVDAGYRVIAPD
CCCCEEEEEECCCEEEEEEECCCCCCCEEEEECCCCHHHHCHHHCCCHHHHCCCEEECCC
QRGYNLSDKPEGIDSYRIDTLRDDIIGLITQFTDEKAIVIGHDWGGAVAWHLASTRPEYL
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCHHHH
EKLIAINIPHPHVMKTVTPLYPPQWLKSSYIAYFQLPDIPEASLRENDYDTLDKAIGLSD
HHHHEECCCCCHHHHHHCCCCCHHHHHCCCEEEEECCCCCCHHHCCCCHHHHHHHCCCCC
RPALFTSEDVSRYKEAWKQPGALTAMLNWYRALRKGSLAEKPSYETVPYRMIWGMEDRFL
CCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHH
SRKLAKETERHCPNGHLIFVDEASHWINHEKPAIVNQLILEYLKNQ
HHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDGVKCQFVNTNGITLHVAAAGREDGPLIVLLHGFPEFWYGWKNQIKPLVDAGYRVIAPD
CCCCEEEEEECCCEEEEEEECCCCCCCEEEEECCCCHHHHCHHHCCCHHHHCCCEEECCC
QRGYNLSDKPEGIDSYRIDTLRDDIIGLITQFTDEKAIVIGHDWGGAVAWHLASTRPEYL
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCHHHH
EKLIAINIPHPHVMKTVTPLYPPQWLKSSYIAYFQLPDIPEASLRENDYDTLDKAIGLSD
HHHHEECCCCCHHHHHHCCCCCHHHHHCCCEEEEECCCCCCHHHCCCCHHHHHHHCCCCC
RPALFTSEDVSRYKEAWKQPGALTAMLNWYRALRKGSLAEKPSYETVPYRMIWGMEDRFL
CCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHH
SRKLAKETERHCPNGHLIFVDEASHWINHEKPAIVNQLILEYLKNQ
HHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377