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The map label for this gene is estB

Identifier: 16077902

GI number: 16077902

Start: 910019

End: 910651

Strand: Direct

Name: estB

Synonym: BSU08350

Alternate gene names: 16077902

Gene position: 910019-910651 (Clockwise)

Preceding gene: 16077900

Following gene: 16077903

Centisome position: 21.59

GC content: 41.07

Gene sequence:

>633_bases
GTGAAAAAAGTACTTATGGCATTCATTATTTGTTTATCGCTGATTCTATCTGTTTTAGCCGCTCCGCCGTCTGGCGCAAA
AGCTGAGTCAGTACATAATCCTGTCGTTCTTGTTCATGGAATAAGTGGTGCATCATACAACTTTTTCGCTATTAAAAACT
ACTTAATTTCTCAAGGCTGGCAAAGCAACAAACTGTACGCAATTGATTTTTATGATAAAACAGGAAACAACCTAAATAAC
GGCCCGCAGCTTGCTTCATATGTTGACCGTGTTTTAAAAGAGACTGGGGCAAAAAAAGTAGATATTGTGGCTCATAGTAT
GGGAGGCGCCAATACGCTGTACTATATTAAATATTTAGGCGGGGGCAATAAGATTCAAAATGTCGTAACGCTTGGAGGGG
CTAATGGTTTAGTGTCATCAACCGCGCTGCCGGGCACAGACCCTAATCAAAAGATCCTCTATACATCTATTTACAGTCTC
AATGATCAAATTGTCATCAATAGCTTGTCTCGGTTACAAGGAGCGCGAAACATCCAGCTTTATGGCATCGGTCATATTGG
CTTGCTTTCTAATAGCCAAGTGAACGGCTATATCAAAGAAGGGCTGAATGGCGGAGGCCTCAATACAAATTAA

Upstream 100 bases:

>100_bases
AAAGAGATTTAAATTTAATTTTCAGAAAAAATAGTAACGATATTGATTTTTTATATATAATAGGAAATTGGTGGTATGAA
AATTTTTTGGGGGAATAAAC

Downstream 100 bases:

>100_bases
TATCTTCAAAAAACAACCTGCTGTCTCCGTTACAGTGGGTTTTTTCGTCTGAGAAAGCCATAGGGTCTTATGACCTTAGT
CTATCATGCATACGTCAATT

Product: secreted esterase / lipase

Products: NA

Alternate protein names: Lipase B; Triacylglycerol lipase

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKKVLMAFIICLSLILSVLAAPPSGAKAESVHNPVVLVHGISGASYNFFAIKNYLISQGWQSNKLYAIDFYDKTGNNLNN
GPQLASYVDRVLKETGAKKVDIVAHSMGGANTLYYIKYLGGGNKIQNVVTLGGANGLVSSTALPGTDPNQKILYTSIYSL
NDQIVINSLSRLQGARNIQLYGIGHIGLLSNSQVNGYIKEGLNGGGLNTN

Sequences:

>Translated_210_residues
MKKVLMAFIICLSLILSVLAAPPSGAKAESVHNPVVLVHGISGASYNFFAIKNYLISQGWQSNKLYAIDFYDKTGNNLNN
GPQLASYVDRVLKETGAKKVDIVAHSMGGANTLYYIKYLGGGNKIQNVVTLGGANGLVSSTALPGTDPNQKILYTSIYSL
NDQIVINSLSRLQGARNIQLYGIGHIGLLSNSQVNGYIKEGLNGGGLNTN
>Mature_210_residues
MKKVLMAFIICLSLILSVLAAPPSGAKAESVHNPVVLVHGISGASYNFFAIKNYLISQGWQSNKLYAIDFYDKTGNNLNN
GPQLASYVDRVLKETGAKKVDIVAHSMGGANTLYYIKYLGGGNKIQNVVTLGGANGLVSSTALPGTDPNQKILYTSIYSL
NDQIVINSLSRLQGARNIQLYGIGHIGLLSNSQVNGYIKEGLNGGGLNTN

Specific function: An esterase which preferentially hydrolyzes triacylglyceride substrates with short chain fatty acids (less than C10) with the maximum activity towards tricaprylin (C8:0). Active against p-nitrophenylesters with fatty acid chain lengths from C6 to C18

COG id: COG1075

COG function: function code R; Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold

Gene ontology:

Cell location: Secreted

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ESTB_BACSU (Q79F14)

Other databases:

- EMBL:   D78508
- EMBL:   AL009126
- RefSeq:   NP_388716.1
- HSSP:   P37957
- ProteinModelPortal:   Q79F14
- SMR:   Q79F14
- EnsemblBacteria:   EBBACT00000003603
- GeneID:   939715
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08350
- NMPDR:   fig|224308.1.peg.835
- GenoList:   BSU08350
- GeneTree:   EBGT00050000002617
- HOGENOM:   HBG338219
- ProtClustDB:   CLSK886633
- BioCyc:   BSUB:BSU08350-MONOMER
- InterPro:   IPR002918

Pfam domain/function: PF01674 Lipase_2

EC number: =3.1.1.3

Molecular weight: Translated: 22363; Mature: 22363

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00120 LIPASE_SER

Important sites: ACT_SITE 106-106 ACT_SITE 162-162 ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLMAFIICLSLILSVLAAPPSGAKAESVHNPVVLVHGISGASYNFFAIKNYLISQGW
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHCCC
QSNKLYAIDFYDKTGNNLNNGPQLASYVDRVLKETGAKKVDIVAHSMGGANTLYYIKYLG
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEEEC
GGNKIQNVVTLGGANGLVSSTALPGTDPNQKILYTSIYSLNDQIVINSLSRLQGARNIQL
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHEEHHHHHCCCCCEEEE
YGIGHIGLLSNSQVNGYIKEGLNGGGLNTN
EEECEEEEECCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKKVLMAFIICLSLILSVLAAPPSGAKAESVHNPVVLVHGISGASYNFFAIKNYLISQGW
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHCCC
QSNKLYAIDFYDKTGNNLNNGPQLASYVDRVLKETGAKKVDIVAHSMGGANTLYYIKYLG
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEEEC
GGNKIQNVVTLGGANGLVSSTALPGTDPNQKILYTSIYSLNDQIVINSLSRLQGARNIQL
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHEEHHHHHCCCCCEEEE
YGIGHIGLLSNSQVNGYIKEGLNGGGLNTN
EEECEEEEECCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377