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The map label for this gene is yfkL

Identifier: 16077853

GI number: 16077853

Start: 860303

End: 861493

Strand: Reverse

Name: yfkL

Synonym: BSU07860

Alternate gene names: 16077853

Gene position: 861493-860303 (Counterclockwise)

Preceding gene: 16077854

Following gene: 16077851

Centisome position: 20.44

GC content: 45.17

Gene sequence:

>1191_bases
ATGCAAACGACATCTATCAAAACAGCATCCGGAATGTATATCAACTACTTCTTTCTGGGGATGGTCAACATCATTCTCGC
ATCCAATATGTCTTCATTAACAAAACAGTGGAATACCGATCCGACCGGCATCAGTTATGTCATTGCCGCTATCGGGTTTG
GCAAACTGCTGACATACGGCATTTCCGGCGTTCTATCAGATAAAATCGGCAGAAAGCCGCTTGTTGTCGCTTCTGCGGGA
ATTATGGCCGTCTTTTTAGTCGGTATTCCTTTATCACCGAGCTATGAGCTGGCGTTTGTGTTCGCTTTATTAGCCGGAGT
CGCTAATTCAGCCATGGATGCCGGAACATATCCGGCCCTAACCGAGCTCTTTCCGTCTGCTTCCGGTTCGGCAAATGTCT
TGGTTAAAGCCTTTATGTCTGTGGGGGCCGCACTTTTGCCGCTTTTGATCACATTTTTAGCAGACCACAGCATGTTTTAC
GGATTTGCTTTTTATCTGCCGGCAGCGGTCTATCTCCTGAATATTATTTATTTATCAACGCTTTCGTTTCCTAAAAAACA
TAAGAAACCAACTAATAGCGGCCAACAGGAATCTCCCGTTTTCTTGTCTGAACCCGTTTTTCAGAAGGAAGGCACCGCAC
TGATTATCATCGGTTTTACATCCACGGCTTTATTTACGGTTTCTCAAATCTGGCTCCCGAGCTACGCGCAAAAGGCAGCC
GGTTTAGCTGAATCAGCATCTGTACAATTGCTGAGCTATTATAGTATAGGATCTCTGGCATCTGTTTTATTATTAGCCGT
TTTGCTGAATAGATGGGTCAAACCTGTCTTCATAACGCTCTTATATCCTATTATTACTTTATGTACGCTCGCAGTCATGC
TGACCGTTCATATCCCAGTTGTGCTGGACATCACTGCCTTTTTCTTAGGCTTTTCAACTGCGGGTGTGTTTCAAATTACC
ATTACGTTAATGACAGAGCTTTTCTGGAAGCGAAAAGGAACCGTTACCGGAATTGTAGCGACTGCTTCAAGTCTTGCATC
GATTCTGCTGCCGATTGCAACAGGGCTTATCGCAAAAGCGGGGGGCATCGCCCATATTTTCATATTCGACTTCGGCATTG
CGGTCATCGGAACGGCTGCAGCTGCTTTCTTGTATTACAGGTACAAGAAACTGACAGGTGCTCAAGCCTAA

Upstream 100 bases:

>100_bases
GTAAATAATGGAGACTGGCCGGAAACTCGGCGAGTCTTTTTTATCATTTCCCCAAACCAAAACAGCAAAGTGTTCGAATA
AGATTTGAAGGAGTACAAGC

Downstream 100 bases:

>100_bases
GCAACAAAAAAACGGACGCCCAAGAGCGTCCGTTTTTTCTTATTTCTCAAGCAATGCCAATGACTCGCGGTTAAATGCCG
GTATATCGTCCGGTGTGCGG

Product: efflux transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MQTTSIKTASGMYINYFFLGMVNIILASNMSSLTKQWNTDPTGISYVIAAIGFGKLLTYGISGVLSDKIGRKPLVVASAG
IMAVFLVGIPLSPSYELAFVFALLAGVANSAMDAGTYPALTELFPSASGSANVLVKAFMSVGAALLPLLITFLADHSMFY
GFAFYLPAAVYLLNIIYLSTLSFPKKHKKPTNSGQQESPVFLSEPVFQKEGTALIIIGFTSTALFTVSQIWLPSYAQKAA
GLAESASVQLLSYYSIGSLASVLLLAVLLNRWVKPVFITLLYPIITLCTLAVMLTVHIPVVLDITAFFLGFSTAGVFQIT
ITLMTELFWKRKGTVTGIVATASSLASILLPIATGLIAKAGGIAHIFIFDFGIAVIGTAAAAFLYYRYKKLTGAQA

Sequences:

>Translated_396_residues
MQTTSIKTASGMYINYFFLGMVNIILASNMSSLTKQWNTDPTGISYVIAAIGFGKLLTYGISGVLSDKIGRKPLVVASAG
IMAVFLVGIPLSPSYELAFVFALLAGVANSAMDAGTYPALTELFPSASGSANVLVKAFMSVGAALLPLLITFLADHSMFY
GFAFYLPAAVYLLNIIYLSTLSFPKKHKKPTNSGQQESPVFLSEPVFQKEGTALIIIGFTSTALFTVSQIWLPSYAQKAA
GLAESASVQLLSYYSIGSLASVLLLAVLLNRWVKPVFITLLYPIITLCTLAVMLTVHIPVVLDITAFFLGFSTAGVFQIT
ITLMTELFWKRKGTVTGIVATASSLASILLPIATGLIAKAGGIAHIFIFDFGIAVIGTAAAAFLYYRYKKLTGAQA
>Mature_396_residues
MQTTSIKTASGMYINYFFLGMVNIILASNMSSLTKQWNTDPTGISYVIAAIGFGKLLTYGISGVLSDKIGRKPLVVASAG
IMAVFLVGIPLSPSYELAFVFALLAGVANSAMDAGTYPALTELFPSASGSANVLVKAFMSVGAALLPLLITFLADHSMFY
GFAFYLPAAVYLLNIIYLSTLSFPKKHKKPTNSGQQESPVFLSEPVFQKEGTALIIIGFTSTALFTVSQIWLPSYAQKAA
GLAESASVQLLSYYSIGSLASVLLLAVLLNRWVKPVFITLLYPIITLCTLAVMLTVHIPVVLDITAFFLGFSTAGVFQIT
ITLMTELFWKRKGTVTGIVATASSLASILLPIATGLIAKAGGIAHIFIFDFGIAVIGTAAAAFLYYRYKKLTGAQA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily

Homologues:

Organism=Escherichia coli, GI226510946, Length=381, Percent_Identity=35.1706036745407, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1787981, Length=404, Percent_Identity=35.6435643564356, Blast_Score=230, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFKL_BACSU (O34597)

Other databases:

- EMBL:   D83967
- EMBL:   AL009126
- PIR:   G69808
- RefSeq:   NP_388667.1
- ProteinModelPortal:   O34597
- EnsemblBacteria:   EBBACT00000003462
- GeneID:   939689
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07860
- NMPDR:   fig|224308.1.peg.786
- GenoList:   BSU07860
- GeneTree:   EBGT00070000032477
- HOGENOM:   HBG338639
- OMA:   APVTGVH
- PhylomeDB:   O34597
- ProtClustDB:   CLSK886870
- BioCyc:   BSUB:BSU07860-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 42169; Mature: 42169

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50850 MFS; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd94cb84)-; HASH(0xb84a034)-; HASH(0xd3c9998)-; HASH(0xd2f58c0)-; HASH(0xdb3ce30)-; HASH(0xd3e2708)-; HASH(0xdabb4cc)-; HASH(0xdbdf0d0)-; HASH(0xda70948)-; HASH(0xdb56ba4)-; HASH(0xd07730c)-; HASH(0xd66f128)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTSIKTASGMYINYFFLGMVNIILASNMSSLTKQWNTDPTGISYVIAAIGFGKLLTYG
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
ISGVLSDKIGRKPLVVASAGIMAVFLVGIPLSPSYELAFVFALLAGVANSAMDAGTYPAL
HHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
TELFPSASGSANVLVKAFMSVGAALLPLLITFLADHSMFYGFAFYLPAAVYLLNIIYLST
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSFPKKHKKPTNSGQQESPVFLSEPVFQKEGTALIIIGFTSTALFTVSQIWLPSYAQKAA
CCCCHHHCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHC
GLAESASVQLLSYYSIGSLASVLLLAVLLNRWVKPVFITLLYPIITLCTLAVMLTVHIPV
CCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLDITAFFLGFSTAGVFQITITLMTELFWKRKGTVTGIVATASSLASILLPIATGLIAKA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GGIAHIFIFDFGIAVIGTAAAAFLYYRYKKLTGAQA
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure
MQTTSIKTASGMYINYFFLGMVNIILASNMSSLTKQWNTDPTGISYVIAAIGFGKLLTYG
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
ISGVLSDKIGRKPLVVASAGIMAVFLVGIPLSPSYELAFVFALLAGVANSAMDAGTYPAL
HHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
TELFPSASGSANVLVKAFMSVGAALLPLLITFLADHSMFYGFAFYLPAAVYLLNIIYLST
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSFPKKHKKPTNSGQQESPVFLSEPVFQKEGTALIIIGFTSTALFTVSQIWLPSYAQKAA
CCCCHHHCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHC
GLAESASVQLLSYYSIGSLASVLLLAVLLNRWVKPVFITLLYPIITLCTLAVMLTVHIPV
CCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLDITAFFLGFSTAGVFQITITLMTELFWKRKGTVTGIVATASSLASILLPIATGLIAKA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GGIAHIFIFDFGIAVIGTAAAAFLYYRYKKLTGAQA
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377