BacMap

Definition	Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession	NC_000964
Length	4,215,606

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The map label for this gene is treA

Identifier: 16077848

GI number: 16077848

Start: 851850

End: 853535

Strand: Direct

Name: treA

Synonym: BSU07810

Alternate gene names: 16077848

Gene position: 851850-853535 (Clockwise)

Preceding gene: 16077847

Following gene: 16077849

Centisome position: 20.21

GC content: 45.97

Gene sequence:

>1686_bases
ATGAAAACAGAACAAACGCCATGGTGGAAAAAAGCTGTGGTTTATCAAATTTATCCGAAAAGCTTTAACGATACGACAGG
AAATGGTGTTGGCGATCTGAACGGCATTATTGAAAAGCTGGATTACTTAAAAACGCTACAGGTGGATGTGCTTTGGCTGA
CGCCGATTTATGATTCTCCCCAGCATGATAATGGGTACGATATTCGTGATTATTACTCGATTTATCCTGAATACGGAACG
ATGGAGGATTTTGAGCGGCTAGTGTCCGAAGCACATAAAAGAGACCTGAAAGTCGTCATGGATCTTGTTGTCAATCATAC
GTCAACAGAGCACAAATGGTTTCGCGAGGCGATCTCTTCAATAGACAGCCCGTACCGTGATTTTTATATATGGAAAAAAC
CGCAGGAAAACGGTTCTGTTCCGACGAACTGGGAATCGAAATTCGGCGGCTCGGCATGGGAGCTTGACGAAGCATCGGGC
CAATACTATTTGCATCTGTTTGATGTGACGCAGGCTGATTTGAATTGGGAAAATGAAGAGGTCAGAAAGCATGTCTATGA
CATGATGCATTTTTGGTTTGAAAAGGGAATAGACGGCTTTAGGCTTGATGTCATCAACCTGATTTCTAAAGATCAGCGCT
TTCCGAATGCGGAGGAAGGCGACGGCCGCTCTTTTTACACTGACGGGCCGCGGGTGCACGAATTTCTGCATGAAATGAAC
GAAAAGGTGTTTTCACATTACGACAGCATGACGGTCGGTGAGATGTCTTCAACAACCGTAGACCACTGCATCCGGTATAC
GAATCCTGACAATAAAGAACTTGATATGACATTCAGCTTCCATCATCTCAAGGTTGACTATCCTAACGGCGAAAAATGGG
CCTTGGCGCCGTTTGATTTTTTGAAGCTGAAGGAGATCCTATCTGACTGGCAAACGGGGATGCACGCAGGAGGGGGATGG
AACGCTCTGTTCTGGTGCAATCATGATCAGCCGCGCGTCGTATCAAGATATGGAGATGATGGCGCATATAGGGTGAAATC
GGCGAAAATGCTTGCTACGGCCATTCATATGATGCAGGGCACGCCTTATATTTATCAGGGCGAGGAGCTGGGGATGACGA
ATCCGAAGTTCACTGACATAAGCTCCTATCGGGATGTCGAATCGCTGAACATGTATCACGCCTTTAAGGAAAAAGGAATG
GCTGATCAAGATATTACAGCCATTTTACAGGCGAAATCCCGTGACAATTCCAGAACCCCCGTTCAGTGGGATGCTACGGA
AAATGGTGGTTTTACAACGGGCACACCATGGATACCGGTTGCAGGAAATTACCGGGAAATCAATGCTGAAGCGGCGCTGA
GGGATCAGAATTCGGTGTTTTACCATTATCAAAAATTGATTCAGATTCGCAAAATGTATGACATTGTGACAGAGGGTACG
TATGAGATAATTGCAAAGGATGACCCGAACATTTTTGCGTATCTCAGACACGGCAGCAATGAAAAGCTGCTCGTGATCAA
TAATTTTTACGGGACAGAGGCGGCCTTTACACTGCCGGACTCTTTAGCGCCTGACGAATGGAAGGCAGAGGTGCTGTTGA
CGAACGATGAAGCAAGGGAAGGGCTTCAGAACATGACGCTTCGCCCATATGAGTCCATTGTTTATCGTTTAACGAAACCG
TGTTAA

Upstream 100 bases:

>100_bases
TACGCGTATGCGAGATTCAAACATAAATAACAAGTGGGGAGCGGGACGTGTTGAACGTTCCCTTTTCCCGTTTTCAATTT
AAATATGGTGGTGGAAACAG

Downstream 100 bases:

>100_bases
ACTAAGCTGGGTGGTCCATAATGAAGGTGAATAAATTCATCACAATTTATAAAGACATCGCACAGCAAATTGAAGGCGGC
CGATGGAAAGCGGAGGAGAT

Product: trehalose-6-phosphate hydrolase

Products: NA

Alternate protein names: Alpha,alpha-phosphotrehalase

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MKTEQTPWWKKAVVYQIYPKSFNDTTGNGVGDLNGIIEKLDYLKTLQVDVLWLTPIYDSPQHDNGYDIRDYYSIYPEYGT
MEDFERLVSEAHKRDLKVVMDLVVNHTSTEHKWFREAISSIDSPYRDFYIWKKPQENGSVPTNWESKFGGSAWELDEASG
QYYLHLFDVTQADLNWENEEVRKHVYDMMHFWFEKGIDGFRLDVINLISKDQRFPNAEEGDGRSFYTDGPRVHEFLHEMN
EKVFSHYDSMTVGEMSSTTVDHCIRYTNPDNKELDMTFSFHHLKVDYPNGEKWALAPFDFLKLKEILSDWQTGMHAGGGW
NALFWCNHDQPRVVSRYGDDGAYRVKSAKMLATAIHMMQGTPYIYQGEELGMTNPKFTDISSYRDVESLNMYHAFKEKGM
ADQDITAILQAKSRDNSRTPVQWDATENGGFTTGTPWIPVAGNYREINAEAALRDQNSVFYHYQKLIQIRKMYDIVTEGT
YEIIAKDDPNIFAYLRHGSNEKLLVINNFYGTEAAFTLPDSLAPDEWKAEVLLTNDEAREGLQNMTLRPYESIVYRLTKP
C

Sequences:

>Translated_561_residues
MKTEQTPWWKKAVVYQIYPKSFNDTTGNGVGDLNGIIEKLDYLKTLQVDVLWLTPIYDSPQHDNGYDIRDYYSIYPEYGT
MEDFERLVSEAHKRDLKVVMDLVVNHTSTEHKWFREAISSIDSPYRDFYIWKKPQENGSVPTNWESKFGGSAWELDEASG
QYYLHLFDVTQADLNWENEEVRKHVYDMMHFWFEKGIDGFRLDVINLISKDQRFPNAEEGDGRSFYTDGPRVHEFLHEMN
EKVFSHYDSMTVGEMSSTTVDHCIRYTNPDNKELDMTFSFHHLKVDYPNGEKWALAPFDFLKLKEILSDWQTGMHAGGGW
NALFWCNHDQPRVVSRYGDDGAYRVKSAKMLATAIHMMQGTPYIYQGEELGMTNPKFTDISSYRDVESLNMYHAFKEKGM
ADQDITAILQAKSRDNSRTPVQWDATENGGFTTGTPWIPVAGNYREINAEAALRDQNSVFYHYQKLIQIRKMYDIVTEGT
YEIIAKDDPNIFAYLRHGSNEKLLVINNFYGTEAAFTLPDSLAPDEWKAEVLLTNDEAREGLQNMTLRPYESIVYRLTKP
C
>Mature_561_residues
MKTEQTPWWKKAVVYQIYPKSFNDTTGNGVGDLNGIIEKLDYLKTLQVDVLWLTPIYDSPQHDNGYDIRDYYSIYPEYGT
MEDFERLVSEAHKRDLKVVMDLVVNHTSTEHKWFREAISSIDSPYRDFYIWKKPQENGSVPTNWESKFGGSAWELDEASG
QYYLHLFDVTQADLNWENEEVRKHVYDMMHFWFEKGIDGFRLDVINLISKDQRFPNAEEGDGRSFYTDGPRVHEFLHEMN
EKVFSHYDSMTVGEMSSTTVDHCIRYTNPDNKELDMTFSFHHLKVDYPNGEKWALAPFDFLKLKEILSDWQTGMHAGGGW
NALFWCNHDQPRVVSRYGDDGAYRVKSAKMLATAIHMMQGTPYIYQGEELGMTNPKFTDISSYRDVESLNMYHAFKEKGM
ADQDITAILQAKSRDNSRTPVQWDATENGGFTTGTPWIPVAGNYREINAEAALRDQNSVFYHYQKLIQIRKMYDIVTEGT
YEIIAKDDPNIFAYLRHGSNEKLLVINNFYGTEAAFTLPDSLAPDEWKAEVLLTNDEAREGLQNMTLRPYESIVYRLTKP
C

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI187423904, Length=550, Percent_Identity=27.2727272727273, Blast_Score=226, Evalue=6e-59,
Organism=Homo sapiens, GI61744477, Length=120, Percent_Identity=31.6666666666667, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI61744475, Length=120, Percent_Identity=31.6666666666667, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI65506891, Length=120, Percent_Identity=31.6666666666667, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI61744479, Length=120, Percent_Identity=31.6666666666667, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI61744481, Length=120, Percent_Identity=31.6666666666667, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI61744483, Length=120, Percent_Identity=31.6666666666667, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1790687, Length=554, Percent_Identity=55.7761732851986, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1786604, Length=193, Percent_Identity=26.4248704663212, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87081873, Length=149, Percent_Identity=25.503355704698, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI25147709, Length=586, Percent_Identity=24.9146757679181, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32565753, Length=221, Percent_Identity=28.9592760180996, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322245, Length=577, Percent_Identity=40.7279029462738, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321726, Length=577, Percent_Identity=38.474870017331, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324416, Length=576, Percent_Identity=39.0625, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6322241, Length=576, Percent_Identity=39.0625, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6322021, Length=576, Percent_Identity=39.0625, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321731, Length=576, Percent_Identity=36.4583333333333, Blast_Score=352, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6319776, Length=576, Percent_Identity=36.2847222222222, Blast_Score=350, Evalue=3e-97,
Organism=Drosophila melanogaster, GI221330053, Length=588, Percent_Identity=34.1836734693878, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI45549022, Length=532, Percent_Identity=34.5864661654135, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24586599, Length=540, Percent_Identity=32.4074074074074, Blast_Score=275, Evalue=7e-74,
Organism=Drosophila melanogaster, GI24586593, Length=582, Percent_Identity=33.6769759450172, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24583745, Length=582, Percent_Identity=32.8178694158076, Blast_Score=269, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24583747, Length=562, Percent_Identity=34.5195729537367, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24583749, Length=562, Percent_Identity=34.5195729537367, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24586597, Length=534, Percent_Identity=31.6479400749064, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24586591, Length=588, Percent_Identity=33.1632653061224, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24586589, Length=583, Percent_Identity=30.0171526586621, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24586587, Length=597, Percent_Identity=29.4807370184255, Blast_Score=241, Evalue=9e-64,
Organism=Drosophila melanogaster, GI281360393, Length=527, Percent_Identity=31.1195445920304, Blast_Score=199, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TREC_BACSU (P39795)

Other databases:

- EMBL:   Z54245
- EMBL:   X80203
- EMBL:   D83967
- EMBL:   AL009126
- PIR:   B69725
- RefSeq:   NP_388662.1
- ProteinModelPortal:   P39795
- SMR:   P39795
- EnsemblBacteria:   EBBACT00000000765
- GeneID:   939690
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07810
- NMPDR:   fig|224308.1.peg.781
- GenoList:   BSU07810
- GeneTree:   EBGT00070000031845
- HOGENOM:   HBG669028
- OMA:   CDSGSKG
- PhylomeDB:   P39795
- ProtClustDB:   CLSK2393370
- BioCyc:   BSUB:BSU07810-MONOMER
- BioCyc:   MetaCyc:MONOMER-5946
- BRENDA:   3.2.1.93
- GO:   GO:0005737
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR012769
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- SMART:   SM00642
- TIGRFAMs:   TIGR02403

Pfam domain/function: PF00128 Alpha-amylase; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.93

Molecular weight: Translated: 65184; Mature: 65184

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: ACT_SITE 203-203 ACT_SITE 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTEQTPWWKKAVVYQIYPKSFNDTTGNGVGDLNGIIEKLDYLKTLQVDVLWLTPIYDSP
CCCCCCCCHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEEECCCCC
QHDNGYDIRDYYSIYPEYGTMEDFERLVSEAHKRDLKVVMDLVVNHTSTEHKWFREAISS
CCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IDSPYRDFYIWKKPQENGSVPTNWESKFGGSAWELDEASGQYYLHLFDVTQADLNWENEE
CCCCCHHEEEEECCCCCCCCCCCCCHHCCCCCEEECCCCCCEEEEEEEEEECCCCCCHHH
VRKHVYDMMHFWFEKGIDGFRLDVINLISKDQRFPNAEEGDGRSFYTDGPRVHEFLHEMN
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
EKVFSHYDSMTVGEMSSTTVDHCIRYTNPDNKELDMTFSFHHLKVDYPNGEKWALAPFDF
HHHHHHHCCCEECCCCHHHHHHHHCCCCCCCCEEEEEEEEEEEEEECCCCCEEEECCHHH
LKLKEILSDWQTGMHAGGGWNALFWCNHDQPRVVSRYGDDGAYRVKSAKMLATAIHMMQG
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHHCC
TPYIYQGEELGMTNPKFTDISSYRDVESLNMYHAFKEKGMADQDITAILQAKSRDNSRTP
CCEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
VQWDATENGGFTTGTPWIPVAGNYREINAEAALRDQNSVFYHYQKLIQIRKMYDIVTEGT
EEEECCCCCCCCCCCCCEECCCCCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
YEIIAKDDPNIFAYLRHGSNEKLLVINNFYGTEAAFTLPDSLAPDEWKAEVLLTNDEARE
EEEEECCCCCEEEEEEECCCCEEEEEECCCCCCEEEECCCCCCCCCCCEEEEEECHHHHH
GLQNMTLRPYESIVYRLTKPC
HHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKTEQTPWWKKAVVYQIYPKSFNDTTGNGVGDLNGIIEKLDYLKTLQVDVLWLTPIYDSP
CCCCCCCCHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEEECCCCC
QHDNGYDIRDYYSIYPEYGTMEDFERLVSEAHKRDLKVVMDLVVNHTSTEHKWFREAISS
CCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IDSPYRDFYIWKKPQENGSVPTNWESKFGGSAWELDEASGQYYLHLFDVTQADLNWENEE
CCCCCHHEEEEECCCCCCCCCCCCCHHCCCCCEEECCCCCCEEEEEEEEEECCCCCCHHH
VRKHVYDMMHFWFEKGIDGFRLDVINLISKDQRFPNAEEGDGRSFYTDGPRVHEFLHEMN
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
EKVFSHYDSMTVGEMSSTTVDHCIRYTNPDNKELDMTFSFHHLKVDYPNGEKWALAPFDF
HHHHHHHCCCEECCCCHHHHHHHHCCCCCCCCEEEEEEEEEEEEEECCCCCEEEECCHHH
LKLKEILSDWQTGMHAGGGWNALFWCNHDQPRVVSRYGDDGAYRVKSAKMLATAIHMMQG
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHCCCCCCEEEHHHHHHHHHHHHHCC
TPYIYQGEELGMTNPKFTDISSYRDVESLNMYHAFKEKGMADQDITAILQAKSRDNSRTP
CCEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
VQWDATENGGFTTGTPWIPVAGNYREINAEAALRDQNSVFYHYQKLIQIRKMYDIVTEGT
EEEECCCCCCCCCCCCCEECCCCCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
YEIIAKDDPNIFAYLRHGSNEKLLVINNFYGTEAAFTLPDSLAPDEWKAEVLLTNDEARE
EEEEECCCCCEEEEEEECCCCEEEEEECCCCCCEEEECCCCCCCCCCCEEEEEECHHHHH
GLQNMTLRPYESIVYRLTKPC
HHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7651129; 8969503; 9384377; 7751281