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The map label for this gene is yflA

Identifier: 16077842

GI number: 16077842

Start: 844770

End: 846185

Strand: Direct

Name: yflA

Synonym: BSU07750

Alternate gene names: 16077842

Gene position: 844770-846185 (Clockwise)

Preceding gene: 255767188

Following gene: 16077847

Centisome position: 20.04

GC content: 46.75

Gene sequence:

>1416_bases
ATGGAACGACTATTAGTATGGATAGAGCATATATCTGATTGGCTGTGGGGTCCGCCGCTAATCATCCTGCTGACGGGGAC
GGGCTTGTATTTCACTATTTTGCTGAAAGGTTTTCAATTTCGCTATCCTTTATACATTTTCAAACAGACAATCGGCAGTG
TAGGAAAAAAGCCGAAGGGAGAAGGCACAGTCACACCGCTTCAGGCATTGACCTCAGCCCTCAGTTCAACGATCGGTGCA
GCGAACATTGTCGGTGTGCCCGCCGCTATTATGTTTGGGGGACCAGGAGCGGTTTTTTGGATGTGGCTTATTGCCTTATT
TGCCATGGCGATTAAGTTTTCTGAAAGTGTTCTTGCTGTTCATTACAGAGAAAAAAATGAGCAGGGTGAATACGTCGGGG
GACCGATGTATTACATAACAAAAGGGCTGCGCATGAAATGGCTCGGCGTATTCTTCTCTGTTGCGCTGATTGTGGAGCTG
ATTCCCAGCATTATGGTTCAGGGAAATTCGGTTTCAGTCTCGCTTGCCGAGACGTTTTCTTTCAATAAAATATACGCAGG
AATCGGAATTGCATTTTTGATTGGGTTAGTGGTGATAGGAGGGGTAAAGCGGATCGGAAAAGTAACAGAGTTTGTCGTGC
CTCTTATGGCAGGGGCATACGCAGGTGCCGGTCTCCTGATTGTTCTCATGAATCTATCATCAGTGCCGGCGTTTTTCTCT
CTCGTTTTTTCAAATGCGTTTACCTCTTCTTCAGCAGTTGGAGGGTTCGCAGGTGCTGCGTTGGCGGAAACCGTTCGCTG
GGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGGATGGGAACAGCCCCGATTGCACATGCGGCAGCAATGACTGATC
ATCCTGTGCGGCAAGGGTTTTGGTCTGTGATCGGCATTGTCATTGACACCTTAATCATCTGTACGACTACCGCTTTTATC
GTCCTTGCATCCGGTGTATGGACAGGGAAGAACGCCTCAAATGACCCGGCAGCACTGACAACGGCCGCATTTCAGCACTA
TTTCGGTTCTGGAGGAGGATACTTCGTTTCGGTTTCCCTGGTCTTCTTTGTCGTATCAACCATTATGGTTGTCATTTTTT
ACGGCGTAAAGCAAGCTGAATTTCTGTTCGGGCGGCTGGCAGGACATGTGATTAAATTCGTTTATTTAGCGGCTATTATC
ATAGGTGCCGCAGGCGGAGCAAAAGCCATTTGGGGCGTATTGGACTTAGCATTGGTTTTTATTGTTGTCCCGAATGTGAT
TGCGCTCTTGTTATTGAGCAGAAAGGTAAAAGCGCTGTACACCGAATTCTTTACATCTGAACAGTACTACCTGAAAGATA
TAAGAAAAACGAAACAAAAGCCTGTTTATCCAACAAAAGAAGCTAAAAATTCCTAG

Upstream 100 bases:

>100_bases
TTGATAAAAGCCATCCTTTTTATGTTCCAAGTTCTATGTCAAAAGGTTTATGTTTTTCCATCTATGGGAAATGATTCATA
AACGAAAAAAGGGTGATGGG

Downstream 100 bases:

>100_bases
TGTTTTGCTTTTGCGCCGAACAATGCTTTGCTTTTTATGATCAAATAAATGAGTAAAGGCAGCAAAAGAGAAATCACTAC
ATTAATGATTGGTTTGATTC

Product: aminoacid transporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MERLLVWIEHISDWLWGPPLIILLTGTGLYFTILLKGFQFRYPLYIFKQTIGSVGKKPKGEGTVTPLQALTSALSSTIGA
ANIVGVPAAIMFGGPGAVFWMWLIALFAMAIKFSESVLAVHYREKNEQGEYVGGPMYYITKGLRMKWLGVFFSVALIVEL
IPSIMVQGNSVSVSLAETFSFNKIYAGIGIAFLIGLVVIGGVKRIGKVTEFVVPLMAGAYAGAGLLIVLMNLSSVPAFFS
LVFSNAFTSSSAVGGFAGAALAETVRWGFARGLYSNEAGMGTAPIAHAAAMTDHPVRQGFWSVIGIVIDTLIICTTTAFI
VLASGVWTGKNASNDPAALTTAAFQHYFGSGGGYFVSVSLVFFVVSTIMVVIFYGVKQAEFLFGRLAGHVIKFVYLAAII
IGAAGGAKAIWGVLDLALVFIVVPNVIALLLLSRKVKALYTEFFTSEQYYLKDIRKTKQKPVYPTKEAKNS

Sequences:

>Translated_471_residues
MERLLVWIEHISDWLWGPPLIILLTGTGLYFTILLKGFQFRYPLYIFKQTIGSVGKKPKGEGTVTPLQALTSALSSTIGA
ANIVGVPAAIMFGGPGAVFWMWLIALFAMAIKFSESVLAVHYREKNEQGEYVGGPMYYITKGLRMKWLGVFFSVALIVEL
IPSIMVQGNSVSVSLAETFSFNKIYAGIGIAFLIGLVVIGGVKRIGKVTEFVVPLMAGAYAGAGLLIVLMNLSSVPAFFS
LVFSNAFTSSSAVGGFAGAALAETVRWGFARGLYSNEAGMGTAPIAHAAAMTDHPVRQGFWSVIGIVIDTLIICTTTAFI
VLASGVWTGKNASNDPAALTTAAFQHYFGSGGGYFVSVSLVFFVVSTIMVVIFYGVKQAEFLFGRLAGHVIKFVYLAAII
IGAAGGAKAIWGVLDLALVFIVVPNVIALLLLSRKVKALYTEFFTSEQYYLKDIRKTKQKPVYPTKEAKNS
>Mature_471_residues
MERLLVWIEHISDWLWGPPLIILLTGTGLYFTILLKGFQFRYPLYIFKQTIGSVGKKPKGEGTVTPLQALTSALSSTIGA
ANIVGVPAAIMFGGPGAVFWMWLIALFAMAIKFSESVLAVHYREKNEQGEYVGGPMYYITKGLRMKWLGVFFSVALIVEL
IPSIMVQGNSVSVSLAETFSFNKIYAGIGIAFLIGLVVIGGVKRIGKVTEFVVPLMAGAYAGAGLLIVLMNLSSVPAFFS
LVFSNAFTSSSAVGGFAGAALAETVRWGFARGLYSNEAGMGTAPIAHAAAMTDHPVRQGFWSVIGIVIDTLIICTTTAFI
VLASGVWTGKNASNDPAALTTAAFQHYFGSGGGYFVSVSLVFFVVSTIMVVIFYGVKQAEFLFGRLAGHVIKFVYLAAII
IGAAGGAKAIWGVLDLALVFIVVPNVIALLLLSRKVKALYTEFFTSEQYYLKDIRKTKQKPVYPTKEAKNS

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family

Homologues:

Organism=Escherichia coli, GI1786188, Length=460, Percent_Identity=34.5652173913044, Blast_Score=224, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFLA_BACSU (O34708)

Other databases:

- EMBL:   D86417
- EMBL:   AL009126
- PIR:   G69809
- RefSeq:   NP_388656.1
- ProteinModelPortal:   O34708
- EnsemblBacteria:   EBBACT00000001680
- GeneID:   939686
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07750
- NMPDR:   fig|224308.1.peg.775
- GenoList:   BSU07750
- GeneTree:   EBGT00070000031806
- HOGENOM:   HBG671706
- OMA:   VQFRYLP
- PhylomeDB:   O34708
- ProtClustDB:   CLSK237442
- BioCyc:   BSUB:BSU07750-MONOMER
- InterPro:   IPR001463
- PRINTS:   PR00175
- TIGRFAMs:   TIGR00835

Pfam domain/function: PF01235 Na_Ala_symp

EC number: NA

Molecular weight: Translated: 50641; Mature: 50641

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdb45a70)-; HASH(0xdb207f8)-; HASH(0xdabaeac)-; HASH(0xd4b83d4)-; HASH(0xdafb528)-; HASH(0xd750f3c)-; HASH(0xda71d48)-; HASH(0xd301be4)-; HASH(0xd2ac97c)-; HASH(0xd825ad0)-; HASH(0xd112628)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLLVWIEHISDWLWGPPLIILLTGTGLYFTILLKGFQFRYPLYIFKQTIGSVGKKPKG
CCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
EGTVTPLQALTSALSSTIGAANIVGVPAAIMFGGPGAVFWMWLIALFAMAIKFSESVLAV
CCCCCHHHHHHHHHHHHCCCHHHHCCCHHEEECCCHHHHHHHHHHHHHHHHHHCCHHEEE
HYREKNEQGEYVGGPMYYITKGLRMKWLGVFFSVALIVELIPSIMVQGNSVSVSLAETFS
EECCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHC
FNKIYAGIGIAFLIGLVVIGGVKRIGKVTEFVVPLMAGAYAGAGLLIVLMNLSSVPAFFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHH
LVFSNAFTSSSAVGGFAGAALAETVRWGFARGLYSNEAGMGTAPIAHAAAMTDHPVRQGF
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
WSVIGIVIDTLIICTTTAFIVLASGVWTGKNASNDPAALTTAAFQHYFGSGGGYFVSVSL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
VFFVVSTIMVVIFYGVKQAEFLFGRLAGHVIKFVYLAAIIIGAAGGAKAIWGVLDLALVF
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IVVPNVIALLLLSRKVKALYTEFFTSEQYYLKDIRKTKQKPVYPTKEAKNS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCH
>Mature Secondary Structure
MERLLVWIEHISDWLWGPPLIILLTGTGLYFTILLKGFQFRYPLYIFKQTIGSVGKKPKG
CCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
EGTVTPLQALTSALSSTIGAANIVGVPAAIMFGGPGAVFWMWLIALFAMAIKFSESVLAV
CCCCCHHHHHHHHHHHHCCCHHHHCCCHHEEECCCHHHHHHHHHHHHHHHHHHCCHHEEE
HYREKNEQGEYVGGPMYYITKGLRMKWLGVFFSVALIVELIPSIMVQGNSVSVSLAETFS
EECCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHC
FNKIYAGIGIAFLIGLVVIGGVKRIGKVTEFVVPLMAGAYAGAGLLIVLMNLSSVPAFFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHH
LVFSNAFTSSSAVGGFAGAALAETVRWGFARGLYSNEAGMGTAPIAHAAAMTDHPVRQGF
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
WSVIGIVIDTLIICTTTAFIVLASGVWTGKNASNDPAALTTAAFQHYFGSGGGYFVSVSL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
VFFVVSTIMVVIFYGVKQAEFLFGRLAGHVIKFVYLAAIIIGAAGGAKAIWGVLDLALVF
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IVVPNVIALLLLSRKVKALYTEFFTSEQYYLKDIRKTKQKPVYPTKEAKNS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377