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The map label for this gene is yclA

Identifier: 16077430

GI number: 16077430

Start: 411578

End: 412450

Strand: Reverse

Name: yclA

Synonym: BSU03620

Alternate gene names: 16077430

Gene position: 412450-411578 (Counterclockwise)

Preceding gene: 255767116

Following gene: 255767112

Centisome position: 9.78

GC content: 45.82

Gene sequence:

>873_bases
TTGGATATTCGCCAGCTTCGATACTTCATTACCATTGCCCAAGAACAGAAAATTACGTCCGCAGCCAAAAAGCTTCACAT
GGCGCAGCCGCCTTTAAGCAGGCAGCTCAAACAGCTGGAGGACGAACTTGGTGTTGTTCTGTTTGACCGTAACAAGAAAA
AACAAATGACTCTTACATATGAAGGAGCTGTTTTTCTAAAAAGAGCAAAAGAGATTCTGCATAGGTTTGAAGATGCAGTC
ATTGAGGTACAGGAGCTGAAGGAGGAAGTAGCCGGTACACTTGCCGTGGGATCAACGATTTATTGCGCGGCTCTGATGCT
TGAAAAAGTAACACAGATCAAAGAGAGATACCCCCATCTCACCTTTAATATTTGGGAAAATGAACCCGCGACACTGTTGG
AACTGCTGGAAAGCCGGCAAATCGATGCGGCGGTCACGACAACACTGATCAAAAGTGACACAGTACAATTCAAGCAGCTT
GATGACATTCCATGTGTGCTCGTGCTTTCAGATGAAGCCGGATATCCGTGCGGAGACACCATTAAGATGGTGGACATTCC
TTCATTTCCGCTGATCCTGCTGCGGCCTGTCAACGGAAAAGGCGTCTATAATCAGGTCATGAATGAATTTCATCGTTTGA
ACCTGGAGCCGCGCATTGTTTGTGAATGCCACGACTCTGCAACTTTGCTCAGCCTCGTGTCATCCGGATTCGGCGCGTCT
ATTTTGCCTGTCACAATGATTCCCGTTCATATGAGAAATCACGTTCACACCGTCCATATCGAAAATAATCCCTTCATTAT
GACACCGGCTGTCATGTGGAGAACGGACAGTTATTTGCCTAAACCGGCACAGCAATTTTTGGATTTATTTTAA

Upstream 100 bases:

>100_bases
TCTGCTTTCATTTCAATCATACTCCTGATATATCCATATGAAAAATATCTATTAAATCTATAATTAATACCTAAAAGGTA
TGATAAGGAGGCGCTTTCTT

Downstream 100 bases:

>100_bases
AAGATTTTGTCTTATGAAAACCAAATACTTGACTCATACTAAAAAAAAGTTATCCTTTAGATTTGTAAGCTAATTTTTTA
TTTCAATAAAATCAACCAGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Bsd operon regulatory protein

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MDIRQLRYFITIAQEQKITSAAKKLHMAQPPLSRQLKQLEDELGVVLFDRNKKKQMTLTYEGAVFLKRAKEILHRFEDAV
IEVQELKEEVAGTLAVGSTIYCAALMLEKVTQIKERYPHLTFNIWENEPATLLELLESRQIDAAVTTTLIKSDTVQFKQL
DDIPCVLVLSDEAGYPCGDTIKMVDIPSFPLILLRPVNGKGVYNQVMNEFHRLNLEPRIVCECHDSATLLSLVSSGFGAS
ILPVTMIPVHMRNHVHTVHIENNPFIMTPAVMWRTDSYLPKPAQQFLDLF

Sequences:

>Translated_290_residues
MDIRQLRYFITIAQEQKITSAAKKLHMAQPPLSRQLKQLEDELGVVLFDRNKKKQMTLTYEGAVFLKRAKEILHRFEDAV
IEVQELKEEVAGTLAVGSTIYCAALMLEKVTQIKERYPHLTFNIWENEPATLLELLESRQIDAAVTTTLIKSDTVQFKQL
DDIPCVLVLSDEAGYPCGDTIKMVDIPSFPLILLRPVNGKGVYNQVMNEFHRLNLEPRIVCECHDSATLLSLVSSGFGAS
ILPVTMIPVHMRNHVHTVHIENNPFIMTPAVMWRTDSYLPKPAQQFLDLF
>Mature_290_residues
MDIRQLRYFITIAQEQKITSAAKKLHMAQPPLSRQLKQLEDELGVVLFDRNKKKQMTLTYEGAVFLKRAKEILHRFEDAV
IEVQELKEEVAGTLAVGSTIYCAALMLEKVTQIKERYPHLTFNIWENEPATLLELLESRQIDAAVTTTLIKSDTVQFKQL
DDIPCVLVLSDEAGYPCGDTIKMVDIPSFPLILLRPVNGKGVYNQVMNEFHRLNLEPRIVCECHDSATLLSLVSSGFGAS
ILPVTMIPVHMRNHVHTVHIENNPFIMTPAVMWRTDSYLPKPAQQFLDLF

Specific function: Could be a positive regulator of bsdBCD expression in response to salicylic acid

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=26.4, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788297, Length=246, Percent_Identity=28.4552845528455, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790399, Length=249, Percent_Identity=24.4979919678715, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788887, Length=264, Percent_Identity=28.7878787878788, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI2367136, Length=246, Percent_Identity=23.5772357723577, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787530, Length=250, Percent_Identity=25.2, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI145693105, Length=293, Percent_Identity=23.2081911262799, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787806, Length=306, Percent_Identity=25.4901960784314, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=287, Percent_Identity=21.9512195121951, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1789204, Length=254, Percent_Identity=24.0157480314961, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789506, Length=289, Percent_Identity=22.1453287197232, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788748, Length=248, Percent_Identity=22.5806451612903, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787601, Length=148, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788296, Length=249, Percent_Identity=24.4979919678715, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789639, Length=248, Percent_Identity=22.1774193548387, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): BSDA_BACSU (P94403)

Other databases:

- EMBL:   D50453
- EMBL:   AL009126
- PIR:   F69761
- RefSeq:   NP_388244.1
- ProteinModelPortal:   P94403
- EnsemblBacteria:   EBBACT00000002947
- GeneID:   938299
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU03620
- NMPDR:   fig|224308.1.peg.363
- GenoList:   BSU03620
- GeneTree:   EBGT00070000031936
- HOGENOM:   HBG685425
- OMA:   ERHIDIG
- PhylomeDB:   P94403
- ProtClustDB:   CLSK886686
- BioCyc:   BSUB:BSU03620-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 32988; Mature: 32988

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRQLRYFITIAQEQKITSAAKKLHMAQPPLSRQLKQLEDELGVVLFDRNKKKQMTLTY
CCHHHEEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEEEE
EGAVFLKRAKEILHRFEDAVIEVQELKEEVAGTLAVGSTIYCAALMLEKVTQIKERYPHL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
TFNIWENEPATLLELLESRQIDAAVTTTLIKSDTVQFKQLDDIPCVLVLSDEAGYPCGDT
EEEEECCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCCCE
IKMVDIPSFPLILLRPVNGKGVYNQVMNEFHRLNLEPRIVCECHDSATLLSLVSSGFGAS
EEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCC
ILPVTMIPVHMRNHVHTVHIENNPFIMTPAVMWRTDSYLPKPAQQFLDLF
CHHHEEEEEECCCCEEEEEECCCCEEECCHHEECCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MDIRQLRYFITIAQEQKITSAAKKLHMAQPPLSRQLKQLEDELGVVLFDRNKKKQMTLTY
CCHHHEEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEEEE
EGAVFLKRAKEILHRFEDAVIEVQELKEEVAGTLAVGSTIYCAALMLEKVTQIKERYPHL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
TFNIWENEPATLLELLESRQIDAAVTTTLIKSDTVQFKQLDDIPCVLVLSDEAGYPCGDT
EEEEECCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCCCE
IKMVDIPSFPLILLRPVNGKGVYNQVMNEFHRLNLEPRIVCECHDSATLLSLVSSGFGAS
EEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCC
ILPVTMIPVHMRNHVHTVHIENNPFIMTPAVMWRTDSYLPKPAQQFLDLF
CHHHEEEEEECCCCEEEEEECCCCEEECCHHEECCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377