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The map label for this gene is glpT

Identifier: 16077283

GI number: 16077283

Start: 233994

End: 235328

Strand: Reverse

Name: glpT

Synonym: BSU02140

Alternate gene names: 16077283

Gene position: 235328-233994 (Counterclockwise)

Preceding gene: 255767058

Following gene: 16077282

Centisome position: 5.58

GC content: 46.44

Gene sequence:

>1335_bases
ATGCTTAATATATTTAAACCGGCTCCTCATATTGAGAGACTGGATGATTCAAAGATGGATGCGGCATACAAACGGCTCAG
ACTGCAAGTATTCATTGGTATTTTCATTGGCTACGCAGGCTACTACCTGCTGCGGAAAAACTTTGCCTTTGCCATTCCTT
ATCTGCAGGAACAGGGATTTTCGAAAACAGAACTGGGTTTGGTGCTGGCCGCTGTGTCCATTGCGTACGGCTTCAGCAAA
TTCATCATGGGAATGGTGTCTGACCGCTGTAATCCGAGATATTTTCTGGCGACAGGCCTATTTTTATCAGCAATCGTTAA
TATTCTATTCGTTTCAATGCCTTGGGTGACTTCGAGCGTCACCATCATGTTTATTTTTATGTTTATCAACGGATGGTTTC
AAGGAATGGGCTGGCCGCCATGCGGCAGGACGATGGCCCATTGGTTCTCCATAAGTGAACGCGGCACAAAAATGTCGATC
TGGAATGTCGCCCATAACATAGGCGGCGGTATTCTTGCTCCGCTTGTCACTCTTGGGATCGCGATGTTCGTCACTTGGAA
AAGCGTGTTTTTCTTCCCTGCCATCATCGCCATTATCATTTCATTTTTAATTGTTTTATTGGTTCGTGATACGCCTCAAT
CCTGCGGACTCCCGCCAATTGAAGAGTATCGGAATGACTATCCAAAACACGCTTTTAAAAATCAGGAAAAAGAATTAACA
ACAAAAGAAATTCTTTTTCAATATGTGCTGAACAACAAATTTCTTTGGTACATCGCTTTTGCCAATGTATTTGTTTATTT
CGTGCGATACGGCGTGGTTGACTGGGCGCCTACTTACTTAACAGAAGCCAAAGGCTTTTCTCCTGAAGACTCACGCTGGT
CGTATTTTCTTTACGAATACGCAGGCATACCGGGAACGATCTTGTGCGGCTGGATCAGTGACCGATTCTTCAAAAGCCGC
CGGGCACCGGCAGGCGTTTTGTTTATGGCGGGTGTTTTCATTGCCGTATTGGTGTACTGGCTGAATCCGGCCGGCAATCC
GCTCGTGGACAATATCGCCCTGATCAGCATTGGCTTCTTAATCTACGGACCTGTTATGCTGATCGGCCTTCAAGCAATTG
ACCTAGCACCGAAAAAAGCAGCCGGAACTGCAGCCGGTTTGACCGGATTTTTCGGATACATCGGCGGATCCGCCTTCGCA
AACGCCATTATGGGCTTTGTCGTGGACCGTTTTAACTGGAACGGCGGCTTTATCATGCTGATCTCATCCTGCATTCTTGC
CATCGTCTTCTTGGCTTTGACGTGGAATACAGGAAAACGGGCAGAGCATGTTTAA

Upstream 100 bases:

>100_bases
CAACCCCTTTTGACAAGGGGTGTTTTCTGGCTCTTTTTGTTTTTCGGGAAAGGAGGCACGCCACACATCCAGCACAAACA
GCAAAGGGGGATAATTATTA

Downstream 100 bases:

>100_bases
GAAAGACACATAAAAGATTAATAGTTTTCCAACACGCCGTTTACATCCGTTTTTTATTATGAAAGACAGGTAGTGCTTAT
CTATAAGGAGGAGAAATCAT

Product: glycerol-3-phosphate permease

Products: Proton [Cytoplasm]; glycerol-3-phosphate [Cytoplasm] [C]

Alternate protein names: G-3-P transporter; G-3-P permease

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MLNIFKPAPHIERLDDSKMDAAYKRLRLQVFIGIFIGYAGYYLLRKNFAFAIPYLQEQGFSKTELGLVLAAVSIAYGFSK
FIMGMVSDRCNPRYFLATGLFLSAIVNILFVSMPWVTSSVTIMFIFMFINGWFQGMGWPPCGRTMAHWFSISERGTKMSI
WNVAHNIGGGILAPLVTLGIAMFVTWKSVFFFPAIIAIIISFLIVLLVRDTPQSCGLPPIEEYRNDYPKHAFKNQEKELT
TKEILFQYVLNNKFLWYIAFANVFVYFVRYGVVDWAPTYLTEAKGFSPEDSRWSYFLYEYAGIPGTILCGWISDRFFKSR
RAPAGVLFMAGVFIAVLVYWLNPAGNPLVDNIALISIGFLIYGPVMLIGLQAIDLAPKKAAGTAAGLTGFFGYIGGSAFA
NAIMGFVVDRFNWNGGFIMLISSCILAIVFLALTWNTGKRAEHV

Sequences:

>Translated_444_residues
MLNIFKPAPHIERLDDSKMDAAYKRLRLQVFIGIFIGYAGYYLLRKNFAFAIPYLQEQGFSKTELGLVLAAVSIAYGFSK
FIMGMVSDRCNPRYFLATGLFLSAIVNILFVSMPWVTSSVTIMFIFMFINGWFQGMGWPPCGRTMAHWFSISERGTKMSI
WNVAHNIGGGILAPLVTLGIAMFVTWKSVFFFPAIIAIIISFLIVLLVRDTPQSCGLPPIEEYRNDYPKHAFKNQEKELT
TKEILFQYVLNNKFLWYIAFANVFVYFVRYGVVDWAPTYLTEAKGFSPEDSRWSYFLYEYAGIPGTILCGWISDRFFKSR
RAPAGVLFMAGVFIAVLVYWLNPAGNPLVDNIALISIGFLIYGPVMLIGLQAIDLAPKKAAGTAAGLTGFFGYIGGSAFA
NAIMGFVVDRFNWNGGFIMLISSCILAIVFLALTWNTGKRAEHV
>Mature_444_residues
MLNIFKPAPHIERLDDSKMDAAYKRLRLQVFIGIFIGYAGYYLLRKNFAFAIPYLQEQGFSKTELGLVLAAVSIAYGFSK
FIMGMVSDRCNPRYFLATGLFLSAIVNILFVSMPWVTSSVTIMFIFMFINGWFQGMGWPPCGRTMAHWFSISERGTKMSI
WNVAHNIGGGILAPLVTLGIAMFVTWKSVFFFPAIIAIIISFLIVLLVRDTPQSCGLPPIEEYRNDYPKHAFKNQEKELT
TKEILFQYVLNNKFLWYIAFANVFVYFVRYGVVDWAPTYLTEAKGFSPEDSRWSYFLYEYAGIPGTILCGWISDRFFKSR
RAPAGVLFMAGVFIAVLVYWLNPAGNPLVDNIALISIGFLIYGPVMLIGLQAIDLAPKKAAGTAAGLTGFFGYIGGSAFA
NAIMGFVVDRFNWNGGFIMLISSCILAIVFLALTWNTGKRAEHV

Specific function: Responsible for glycerol-3-phosphate uptake

COG id: COG2271

COG function: function code G; Sugar phosphate permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family

Homologues:

Organism=Homo sapiens, GI256219598, Length=406, Percent_Identity=29.3103448275862, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI4503847, Length=406, Percent_Identity=29.3103448275862, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI256219483, Length=406, Percent_Identity=29.3103448275862, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI256219543, Length=428, Percent_Identity=28.0373831775701, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI256219564, Length=337, Percent_Identity=27.299703264095, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI223717983, Length=401, Percent_Identity=26.6832917705736, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI38093649, Length=401, Percent_Identity=26.6832917705736, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI46361976, Length=388, Percent_Identity=23.9690721649485, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1788573, Length=443, Percent_Identity=60.4966139954853, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI87082310, Length=427, Percent_Identity=35.3629976580796, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI2367259, Length=417, Percent_Identity=32.6139088729017, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI2367379, Length=417, Percent_Identity=23.5011990407674, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789152, Length=412, Percent_Identity=23.7864077669903, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87082320, Length=403, Percent_Identity=24.5657568238213, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789515, Length=455, Percent_Identity=22.8571428571429, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17564202, Length=430, Percent_Identity=24.6511627906977, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17564204, Length=367, Percent_Identity=25.3405994550409, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI133930953, Length=466, Percent_Identity=23.3905579399142, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI133901854, Length=481, Percent_Identity=23.0769230769231, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI133901856, Length=481, Percent_Identity=23.0769230769231, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17561094, Length=392, Percent_Identity=22.1938775510204, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24656866, Length=495, Percent_Identity=24.6464646464646, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI45550478, Length=495, Percent_Identity=24.6464646464646, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24656861, Length=479, Percent_Identity=24.8434237995825, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLPT_BACSU (P37948)

Other databases:

- EMBL:   Z26522
- EMBL:   AB006424
- EMBL:   AL009126
- PIR:   I40417
- RefSeq:   NP_388096.1
- ProteinModelPortal:   P37948
- SMR:   P37948
- EnsemblBacteria:   EBBACT00000003479
- GeneID:   938447
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU02140
- NMPDR:   fig|224308.1.peg.215
- GenoList:   BSU02140
- GeneTree:   EBGT00050000000112
- HOGENOM:   HBG559184
- OMA:   PPDEKIK
- PhylomeDB:   P37948
- ProtClustDB:   CLSK886607
- BioCyc:   BSUB:BSU02140-MONOMER
- InterPro:   IPR005267
- InterPro:   IPR000849
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR021159
- TIGRFAMs:   TIGR00881
- TIGRFAMs:   TIGR00712

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 49802; Mature: 49802

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS00942 GLPT; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc0f1c14)-; HASH(0xc02c094)-; HASH(0xb44b730)-; HASH(0xc6b1dac)-; HASH(0xbbd821c)-; HASH(0xb3200c0)-; HASH(0xc0f6bcc)-; HASH(0xb46f28c)-; HASH(0xc1bc0f0)-; HASH(0xc018afc)-; HASH(0xaf7e2bc)-; HASH(0xc28c328)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCCH
>Mature Secondary Structure
MLNIFKPAPHIERLDDSKMDAAYKRLRLQVFIGIFIGYAGYYLLRKNFAFAIPYLQEQGF
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHCCC
SKTELGLVLAAVSIAYGFSKFIMGMVSDRCNPRYFLATGLFLSAIVNILFVSMPWVTSSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
TIMFIFMFINGWFQGMGWPPCGRTMAHWFSISERGTKMSIWNVAHNIGGGILAPLVTLGI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHHHH
AMFVTWKSVFFFPAIIAIIISFLIVLLVRDTPQSCGLPPIEEYRNDYPKHAFKNQEKELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHCCCHHHHH
TKEILFQYVLNNKFLWYIAFANVFVYFVRYGVVDWAPTYLTEAKGFSPEDSRWSYFLYEY
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEEE
AGIPGTILCGWISDRFFKSRRAPAGVLFMAGVFIAVLVYWLNPAGNPLVDNIALISIGFL
CCCCHHEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IYGPVMLIGLQAIDLAPKKAAGTAAGLTGFFGYIGGSAFANAIMGFVVDRFNWNGGFIML
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHH
ISSCILAIVFLALTWNTGKRAEHV
HHHHHHHHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; glycerol-3-phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + glycerol-3-phosphate [Periplasm] = Proton [Cytoplasm] + glycerol-3-phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8012593; 9384377