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The map label for this gene is rlmI [H]

Identifier: 159901508

GI number: 159901508

Start: 6338630

End: 6339634

Strand: Direct

Name: rlmI [H]

Synonym: Haur_4997

Alternate gene names: 159901508

Gene position: 6338630-6339634 (Clockwise)

Preceding gene: 159901507

Following gene: 159901509

Centisome position: 99.87

GC content: 50.25

Gene sequence:

>1005_bases
ATGCCAGTCGTGACTTTGACTGATCGAATAGAATGGGCAATTGTTGCTCGTGAAAGCTTGTTTGACGAACGCCATCAGGC
AGGCTTGCGCTTGTTCAATGGGTTTCTTGAGGGCGAGCCGCAACTTGTGATCGACCTGTATGCGACCACAGCGGTGGTGC
ATAACTATGCCGACCCGCCCCAAGCAGGCGAGGCCTTGGTTGAGCAAGTGCTGGCCTGGTTGCCAACTCGCTTGCCATGG
CTCGAAGCAATTTTAGTCAAAACCCGCCATAGCGATGATGAAACAGCTCAAAATGGGGTTTTGCGCTATGGCACAAGTTT
GGCGACGCGCATCCGTGAGCATGGAGTTTGGTACGCGCTTGATCTGACTATGAACCGCGATGCCAGCTTGTATTTGGATA
CCCGCAACTTGCGCCAGTGGATTTTGGCTAATTTGGCCGATAAAACGGTGCTCAACACCTTTGCCTATACGGGAAGCTTA
GGCGTGGCGGCGGTGGCGGCGGGAGCCAAACGGGTAGTACAAACCGACCGTAATCGGCGCTTTTTGAATGTGGCCAAAAC
CTCGCACACCCTGAATGGCTTTCCAATTCATAAACCCGATTTTCAAACTGACGATTTTTGGCTGCACATGAATAAATTCA
AACGCAAAGACGAGTTGTTCGATTGTGTGATTGTTGACCCGCCGATGTTTGCTGCCACCGACCATGGGGTGGTCGATTTG
ACCCAAAACTACACTAAGGTGCTCAATCGAGTGCGGCCTTTGATGGAGCACAACGGCACAATTATCGCAATCAACAATGC
CCTATTCTTGAGCGGCGAAGAATATCTCGCCTTGCTTGAAAAAATTTGTGCCGATGGCTACGTTGAGGTTGAACAATATA
TTGATATCGCTGAAGATTTTACGGGCTACCCAACAACGCGGCTTAGCAAACCAATCACCAATCCCTCACCGTTTAATCAT
TCGACCAAAATTGCCGTGCTCAAAACCCGCCGCCGTGGATTTTAA

Upstream 100 bases:

>100_bases
ATTTTAATTTAACAAGGCTTGAGCCAATTCGATCCTTAATTGTATTTCGGTTAAAATAGCATAACGCCAACGATCTGTTG
ACAGCAATTAAGGATGAATT

Downstream 100 bases:

>100_bases
GTAAAGTCAAAAGGCCGAAATCAAAAGGCAAAAGTAGCGCTAACAAATAATCAAGGGAAATTTAACGCAAAGGCGCAGAG
TGGGGTTTGGCTATAGGCTA

Product: SAM-dependent methyltransferase-like protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MPVVTLTDRIEWAIVARESLFDERHQAGLRLFNGFLEGEPQLVIDLYATTAVVHNYADPPQAGEALVEQVLAWLPTRLPW
LEAILVKTRHSDDETAQNGVLRYGTSLATRIREHGVWYALDLTMNRDASLYLDTRNLRQWILANLADKTVLNTFAYTGSL
GVAAVAAGAKRVVQTDRNRRFLNVAKTSHTLNGFPIHKPDFQTDDFWLHMNKFKRKDELFDCVIVDPPMFAATDHGVVDL
TQNYTKVLNRVRPLMEHNGTIIAINNALFLSGEEYLALLEKICADGYVEVEQYIDIAEDFTGYPTTRLSKPITNPSPFNH
STKIAVLKTRRRGF

Sequences:

>Translated_334_residues
MPVVTLTDRIEWAIVARESLFDERHQAGLRLFNGFLEGEPQLVIDLYATTAVVHNYADPPQAGEALVEQVLAWLPTRLPW
LEAILVKTRHSDDETAQNGVLRYGTSLATRIREHGVWYALDLTMNRDASLYLDTRNLRQWILANLADKTVLNTFAYTGSL
GVAAVAAGAKRVVQTDRNRRFLNVAKTSHTLNGFPIHKPDFQTDDFWLHMNKFKRKDELFDCVIVDPPMFAATDHGVVDL
TQNYTKVLNRVRPLMEHNGTIIAINNALFLSGEEYLALLEKICADGYVEVEQYIDIAEDFTGYPTTRLSKPITNPSPFNH
STKIAVLKTRRRGF
>Mature_333_residues
PVVTLTDRIEWAIVARESLFDERHQAGLRLFNGFLEGEPQLVIDLYATTAVVHNYADPPQAGEALVEQVLAWLPTRLPWL
EAILVKTRHSDDETAQNGVLRYGTSLATRIREHGVWYALDLTMNRDASLYLDTRNLRQWILANLADKTVLNTFAYTGSLG
VAAVAAGAKRVVQTDRNRRFLNVAKTSHTLNGFPIHKPDFQTDDFWLHMNKFKRKDELFDCVIVDPPMFAATDHGVVDLT
QNYTKVLNRVRPLMEHNGTIIAINNALFLSGEEYLALLEKICADGYVEVEQYIDIAEDFTGYPTTRLSKPITNPSPFNHS
TKIAVLKTRRRGF

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=272, Percent_Identity=23.8970588235294, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787181, Length=247, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 37842; Mature: 37710

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVTLTDRIEWAIVARESLFDERHQAGLRLFNGFLEGEPQLVIDLYATTAVVHNYADPP
CCEEEEECCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCCCC
QAGEALVEQVLAWLPTRLPWLEAILVKTRHSDDETAQNGVLRYGTSLATRIREHGVWYAL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHCCCEEEE
DLTMNRDASLYLDTRNLRQWILANLADKTVLNTFAYTGSLGVAAVAAGAKRVVQTDRNRR
EEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCE
FLNVAKTSHTLNGFPIHKPDFQTDDFWLHMNKFKRKDELFDCVIVDPPMFAATDHGVVDL
EEEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHCCHHHHEEEEEECCCCEECCCCCEEEH
TQNYTKVLNRVRPLMEHNGTIIAINNALFLSGEEYLALLEKICADGYVEVEQYIDIAEDF
HHHHHHHHHHHHHHHHCCCEEEEECCEEEECCHHHHHHHHHHHCCCCEEHHHHHHHHHHC
TGYPTTRLSKPITNPSPFNHSTKIAVLKTRRRGF
CCCCCHHCCCCCCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
PVVTLTDRIEWAIVARESLFDERHQAGLRLFNGFLEGEPQLVIDLYATTAVVHNYADPP
CEEEEECCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCCCC
QAGEALVEQVLAWLPTRLPWLEAILVKTRHSDDETAQNGVLRYGTSLATRIREHGVWYAL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHCCCEEEE
DLTMNRDASLYLDTRNLRQWILANLADKTVLNTFAYTGSLGVAAVAAGAKRVVQTDRNRR
EEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCE
FLNVAKTSHTLNGFPIHKPDFQTDDFWLHMNKFKRKDELFDCVIVDPPMFAATDHGVVDL
EEEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHCCHHHHEEEEEECCCCEECCCCCEEEH
TQNYTKVLNRVRPLMEHNGTIIAINNALFLSGEEYLALLEKICADGYVEVEQYIDIAEDF
HHHHHHHHHHHHHHHHCCCEEEEECCEEEECCHHHHHHHHHHHCCCCEEHHHHHHHHHHC
TGYPTTRLSKPITNPSPFNHSTKIAVLKTRRRGF
CCCCCHHCCCCCCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA