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The map label for this gene is truA [H]

Identifier: 159901453

GI number: 159901453

Start: 6269369

End: 6270187

Strand: Direct

Name: truA [H]

Synonym: Haur_4942

Alternate gene names: 159901453

Gene position: 6269369-6270187 (Clockwise)

Preceding gene: 159901452

Following gene: 159901454

Centisome position: 98.78

GC content: 54.95

Gene sequence:

>819_bases
ATGAGCCGTACTTTAGCATTGTGTTTTGAATATCTCGGCACTGCTTTTTGCGGCTCGCAATGGCAACCAGGCGGTCGCGC
CGTGCAAACGGAGTTAGAAACCGCTTGGCAACGATTAACAAGCGAAACTGGGCGTTGGATTTTTGCCGGTCGCACCGATG
CAGGAGTTCACGCCCTAGCCCAAGTCGCCCATATCGTGAGCGATACGCCGCGCAGTTTGGATACAATTGCCAAGGCAATC
AATGCCATTACGCCGCCCGATATCAGTGTTCACGAAGCATGGGAGATGCCAACTGGCTTTCACGCTCGCTTTAGTGCCCG
TCAACGAACATATCGCTATGTTTTTGATAGTGCACCTGCACCTTCAGCTACGCTCTCTGGGCGGGTGCTGCGCCTCGATC
ATCAACTAGATTGGGCGGCGATGCAGGCGGCCTTGGATACCCTGATTGGCACACACGATTTTGCGGCGTTCGCTGGCGCT
GGTCACGAAGGCTCGACAACTCGCACCTGTATGTTGGCGCAATTACGCCCCGCAGAGTGGCTTGGTCGCCCAGTGACTGT
CCTGCATTTGCAGGCCAATGCTTTTCTCAAGCATATGGTGCGCAATATCGTTGGTACGTTGCTGATGGTTGGCGATGGCC
GGCTTTCATTGGAAGCTTGGCAGGCAATTATTGCCAGTCGCGACCGTCGTAACGCAGGCCCAACCGCGCCCCCACAGGGG
TTGTATTTAGAGTCGATTGGCTATGACCCAGCACTTCAGCCATTGGCGACAAGTTCACGCTGGGATGGCACGACCTATTT
TCGGACGTATCAAGGCTAA

Upstream 100 bases:

>100_bases
ACGCCGAACGCCCAGGTGGTTACACGCGGATTGTCAAGATGGGCTTCCGCAAAGGCGACGCTGCGGAAATGGCTCGGATT
GAATTGGTCTAGCTTGCCCT

Downstream 100 bases:

>100_bases
CGATGTGAATACGGGCAGGTTGCCAACGCTTCTCATCGCCAACCTTACAACGATTTGTGAACATGCCAGCGTGGCATAAT
TCACAATTCATACACGAGGA

Product: tRNA pseudouridine synthase ACD

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSRTLALCFEYLGTAFCGSQWQPGGRAVQTELETAWQRLTSETGRWIFAGRTDAGVHALAQVAHIVSDTPRSLDTIAKAI
NAITPPDISVHEAWEMPTGFHARFSARQRTYRYVFDSAPAPSATLSGRVLRLDHQLDWAAMQAALDTLIGTHDFAAFAGA
GHEGSTTRTCMLAQLRPAEWLGRPVTVLHLQANAFLKHMVRNIVGTLLMVGDGRLSLEAWQAIIASRDRRNAGPTAPPQG
LYLESIGYDPALQPLATSSRWDGTTYFRTYQG

Sequences:

>Translated_272_residues
MSRTLALCFEYLGTAFCGSQWQPGGRAVQTELETAWQRLTSETGRWIFAGRTDAGVHALAQVAHIVSDTPRSLDTIAKAI
NAITPPDISVHEAWEMPTGFHARFSARQRTYRYVFDSAPAPSATLSGRVLRLDHQLDWAAMQAALDTLIGTHDFAAFAGA
GHEGSTTRTCMLAQLRPAEWLGRPVTVLHLQANAFLKHMVRNIVGTLLMVGDGRLSLEAWQAIIASRDRRNAGPTAPPQG
LYLESIGYDPALQPLATSSRWDGTTYFRTYQG
>Mature_271_residues
SRTLALCFEYLGTAFCGSQWQPGGRAVQTELETAWQRLTSETGRWIFAGRTDAGVHALAQVAHIVSDTPRSLDTIAKAIN
AITPPDISVHEAWEMPTGFHARFSARQRTYRYVFDSAPAPSATLSGRVLRLDHQLDWAAMQAALDTLIGTHDFAAFAGAG
HEGSTTRTCMLAQLRPAEWLGRPVTVLHLQANAFLKHMVRNIVGTLLMVGDGRLSLEAWQAIIASRDRRNAGPTAPPQGL
YLESIGYDPALQPLATSSRWDGTTYFRTYQG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=271, Percent_Identity=33.9483394833948, Blast_Score=104, Evalue=6e-23,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=37.8048780487805, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29838; Mature: 29707

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTLALCFEYLGTAFCGSQWQPGGRAVQTELETAWQRLTSETGRWIFAGRTDAGVHALA
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHH
QVAHIVSDTPRSLDTIAKAINAITPPDISVHEAWEMPTGFHARFSARQRTYRYVFDSAPA
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHEECCCCC
PSATLSGRVLRLDHQLDWAAMQAALDTLIGTHDFAAFAGAGHEGSTTRTCMLAQLRPAEW
CCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHEEHHHHCCCHHH
LGRPVTVLHLQANAFLKHMVRNIVGTLLMVGDGRLSLEAWQAIIASRDRRNAGPTAPPQG
CCCCEEEEEECHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
LYLESIGYDPALQPLATSSRWDGTTYFRTYQG
CEEECCCCCCCCCHHCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
SRTLALCFEYLGTAFCGSQWQPGGRAVQTELETAWQRLTSETGRWIFAGRTDAGVHALA
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHH
QVAHIVSDTPRSLDTIAKAINAITPPDISVHEAWEMPTGFHARFSARQRTYRYVFDSAPA
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHEECCCCC
PSATLSGRVLRLDHQLDWAAMQAALDTLIGTHDFAAFAGAGHEGSTTRTCMLAQLRPAEW
CCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHEEHHHHCCCHHH
LGRPVTVLHLQANAFLKHMVRNIVGTLLMVGDGRLSLEAWQAIIASRDRRNAGPTAPPQG
CCCCEEEEEECHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
LYLESIGYDPALQPLATSSRWDGTTYFRTYQG
CEEECCCCCCCCCHHCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA