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The map label for this gene is nodE [H]

Identifier: 159901422

GI number: 159901422

Start: 6249978

End: 6251174

Strand: Reverse

Name: nodE [H]

Synonym: Haur_4911

Alternate gene names: 159901422

Gene position: 6251174-6249978 (Counterclockwise)

Preceding gene: 159901456

Following gene: 159901421

Centisome position: 98.5

GC content: 53.88

Gene sequence:

>1197_bases
ATGAAACAGCGAGTTGTCATCACCGGAATCGGGATTTGCAGCGCCCTTGGGAATGATATTGAAGCGTATTGGGCGGCCTT
GTTGGCAGGCCAAAGCGGGGTCAAGCCACTGCCCGACCTAGCACCCTACAAAGATCCGGTGGTTGGTGGCGCAACCCCCG
ATTTACCATTGCTCACCAGCCATCGCCGCCAGCCTGATCGTATGATCAGCTTGGCCCATCATGCCGTGGAGCAAGCGATT
ACCATGGCCAACGGCCTGCCTCAAGATACCGCTGTCTTATGGGGCACAGGCTATACCACGATTTCGACGATCGAAGCTTG
CTACGAAAATTGGTTCACCAATGGCAAAGTACGGGCAGATACCGTGCCAGCCTGTATGCCCGCCGCCGTGCTGCCACATC
TTTGTACCGTCTTTGATTTGCACGGCCCAAGCACCTTAATTTCAGCAACCTGCGCCTCAGGCTTGCAAGCGATTGGCATT
GCCGCCGATCTGATTCGGGCTGGCGTGATTGAAACTGCGGTTGCTGGAGCCTCCGATGCGCCACTTACCAAGGGCATGTA
TGCAGCATGGAAGCCCTTGCGCGTACTCGCCAGCGACCTGAGCGACCCAAGTCGTGCCTGCCGCCCTTTCAGCGCTGATC
GCAATGGTTTAGTCTTGGCGGAAGGTGCCGGCGCATTTGTGCTCGAAAGTTTGGAGCATGCCAAAGCCCGCAATGCACCC
ATTTTAGCCGAGATTATGGCCTCAGCTGCGACGACCGCCAGTGGGAGCATTGTGGCACCCGATTTAGAACACCAAACATT
AACATTAACTAAAGCTTTAGCACGTGCCCAAATTAGCATTCACGATGTCGATTATATTAATGCCCATGCCACTGGTACCA
AGCTGGGCGATGCCGCTGAAGCCGCTACCATCAAAGCAGTTTTTGGTGAACACGCCAAACATGTGCCAATTTCAGCGATC
AAAGGTGCAACCGGCCATGCGATGGGTGCAAGCTCAGCGCTCGAAGCAGTTGCGACGATCTTAGCTTTACGCGATCAAGT
GCTACCGCCAACAATTAATTGGCAAGCAGGCGATCCTGATTGTGATTTGGATTTTGTGGTTGAAGGTGCTCGCCAGCAAA
CAATTAATCTCGCAGTTAAAGAATCGTTTGGGTTTGGTGGCTCCAACGCCGTTTTAGTGCTGCGTCGCTGGGTATAA

Upstream 100 bases:

>100_bases
ACTCCCCGCTACAATGAACAGTAGCATCATAACCCCGAAACCACCAAGCCATACCACGACAATGGAGCCTGTGGTAGGTC
GGCAGCGCAGAGGAGCCTCT

Downstream 100 bases:

>100_bases
TTGACGCAGTAAAATCGCCACCAGTATAATTTCATTCACGCACCACCATCGAATTGGAGGGCTTATGGCAGCCGACAAAG
ACGCATTATTAAAAGAATTG

Product: beta-ketoacyl synthase

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: Host-specificity of nodulation protein B [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKQRVVITGIGICSALGNDIEAYWAALLAGQSGVKPLPDLAPYKDPVVGGATPDLPLLTSHRRQPDRMISLAHHAVEQAI
TMANGLPQDTAVLWGTGYTTISTIEACYENWFTNGKVRADTVPACMPAAVLPHLCTVFDLHGPSTLISATCASGLQAIGI
AADLIRAGVIETAVAGASDAPLTKGMYAAWKPLRVLASDLSDPSRACRPFSADRNGLVLAEGAGAFVLESLEHAKARNAP
ILAEIMASAATTASGSIVAPDLEHQTLTLTKALARAQISIHDVDYINAHATGTKLGDAAEAATIKAVFGEHAKHVPISAI
KGATGHAMGASSALEAVATILALRDQVLPPTINWQAGDPDCDLDFVVEGARQQTINLAVKESFGFGGSNAVLVLRRWV

Sequences:

>Translated_398_residues
MKQRVVITGIGICSALGNDIEAYWAALLAGQSGVKPLPDLAPYKDPVVGGATPDLPLLTSHRRQPDRMISLAHHAVEQAI
TMANGLPQDTAVLWGTGYTTISTIEACYENWFTNGKVRADTVPACMPAAVLPHLCTVFDLHGPSTLISATCASGLQAIGI
AADLIRAGVIETAVAGASDAPLTKGMYAAWKPLRVLASDLSDPSRACRPFSADRNGLVLAEGAGAFVLESLEHAKARNAP
ILAEIMASAATTASGSIVAPDLEHQTLTLTKALARAQISIHDVDYINAHATGTKLGDAAEAATIKAVFGEHAKHVPISAI
KGATGHAMGASSALEAVATILALRDQVLPPTINWQAGDPDCDLDFVVEGARQQTINLAVKESFGFGGSNAVLVLRRWV
>Mature_398_residues
MKQRVVITGIGICSALGNDIEAYWAALLAGQSGVKPLPDLAPYKDPVVGGATPDLPLLTSHRRQPDRMISLAHHAVEQAI
TMANGLPQDTAVLWGTGYTTISTIEACYENWFTNGKVRADTVPACMPAAVLPHLCTVFDLHGPSTLISATCASGLQAIGI
AADLIRAGVIETAVAGASDAPLTKGMYAAWKPLRVLASDLSDPSRACRPFSADRNGLVLAEGAGAFVLESLEHAKARNAP
ILAEIMASAATTASGSIVAPDLEHQTLTLTKALARAQISIHDVDYINAHATGTKLGDAAEAATIKAVFGEHAKHVPISAI
KGATGHAMGASSALEAVATILALRDQVLPPTINWQAGDPDCDLDFVVEGARQQTINLAVKESFGFGGSNAVLVLRRWV

Specific function: Proposed to synthesize NOD factor fatty acyl chain. Involved in the synthesis of a highly unsaturated fatty acid moiety, which forms part of a lipo-oligosaccharide that is responsible for host specificity [H]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=418, Percent_Identity=34.2105263157895, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI223972627, Length=146, Percent_Identity=43.1506849315069, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI41872631, Length=284, Percent_Identity=27.112676056338, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787337, Length=411, Percent_Identity=35.2798053527981, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1788663, Length=411, Percent_Identity=32.6034063260341, Blast_Score=163, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71983541, Length=408, Percent_Identity=30.6372549019608, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI71983535, Length=265, Percent_Identity=34.7169811320755, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI212642053, Length=282, Percent_Identity=29.4326241134752, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17550940, Length=264, Percent_Identity=26.8939393939394, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320904, Length=448, Percent_Identity=30.8035714285714, Blast_Score=179, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24644341, Length=421, Percent_Identity=34.916864608076, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI19920632, Length=420, Percent_Identity=24.5238095238095, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI221330659, Length=420, Percent_Identity=24.5238095238095, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24581345, Length=420, Percent_Identity=24.5238095238095, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 41467; Mature: 41467

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00098 THIOLASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQRVVITGIGICSALGNDIEAYWAALLAGQSGVKPLPDLAPYKDPVVGGATPDLPLLTS
CCCCEEEEECHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHC
HRRQPDRMISLAHHAVEQAITMANGLPQDTAVLWGTGYTTISTIEACYENWFTNGKVRAD
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEC
TVPACMPAAVLPHLCTVFDLHGPSTLISATCASGLQAIGIAADLIRAGVIETAVAGASDA
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PLTKGMYAAWKPLRVLASDLSDPSRACRPFSADRNGLVLAEGAGAFVLESLEHAKARNAP
CCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCC
ILAEIMASAATTASGSIVAPDLEHQTLTLTKALARAQISIHDVDYINAHATGTKLGDAAE
HHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCHHH
AATIKAVFGEHAKHVPISAIKGATGHAMGASSALEAVATILALRDQVLPPTINWQAGDPD
HHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
CDLDFVVEGARQQTINLAVKESFGFGGSNAVLVLRRWV
CCHHHHCCCCHHHEEEEEEEECCCCCCCCEEEEEEECC
>Mature Secondary Structure
MKQRVVITGIGICSALGNDIEAYWAALLAGQSGVKPLPDLAPYKDPVVGGATPDLPLLTS
CCCCEEEEECHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHC
HRRQPDRMISLAHHAVEQAITMANGLPQDTAVLWGTGYTTISTIEACYENWFTNGKVRAD
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEC
TVPACMPAAVLPHLCTVFDLHGPSTLISATCASGLQAIGIAADLIRAGVIETAVAGASDA
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PLTKGMYAAWKPLRVLASDLSDPSRACRPFSADRNGLVLAEGAGAFVLESLEHAKARNAP
CCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCC
ILAEIMASAATTASGSIVAPDLEHQTLTLTKALARAQISIHDVDYINAHATGTKLGDAAE
HHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCHHH
AATIKAVFGEHAKHVPISAIKGATGHAMGASSALEAVATILALRDQVLPPTINWQAGDPD
HHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
CDLDFVVEGARQQTINLAVKESFGFGGSNAVLVLRRWV
CCHHHHCCCCHHHEEEEEEEECCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9100384 [H]