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The map label for this gene is pgk

Identifier: 159901356

GI number: 159901356

Start: 6176313

End: 6177497

Strand: Direct

Name: pgk

Synonym: Haur_4845

Alternate gene names: 159901356

Gene position: 6176313-6177497 (Clockwise)

Preceding gene: 159901355

Following gene: 159901357

Centisome position: 97.32

GC content: 54.26

Gene sequence:

>1185_bases
GTGAACAAGCAGACCATTCGTGATGTAGCGTGGAGCGGAAAACGAGCTTTGGTTCGGGTGGATTTCAATGTGCCGCTTGA
TCAAGCCCGTAAAGTCACTGATGACACTCGGATTCGGGCAGCGTTGCCTACGATCAACTATTTGCTCGAACAAGGTGCAA
GTGTGATTTTGATGTCGCACTTGGGCCGCCCGAAGAAAAAAGTGGCCGAAGAATTCCGGCTCAAGCCAGTTGCCGATCAT
CTCCAATCGCTGCTGGATGCCCCAGTGAACTATATTCAGACAACGACTGGCGCTGAGGCCGAAGCTGCTGCCCAAGCCTT
ACAAGCAGGCCAAGTGCTGTTGCTGGAAAATACCCGCTTCGACCCCCGCGAAGAATCCAACGACCCCGCCATGGCCGAAG
AATTGGCCAAACTTGGCGATATTTATGTGAATGATGCTTTTGGGGCGGCCCACCGTGCTCATGCCTCAACCGAAGGCCTC
GCCAAATTCCTGCCAGCCGTGGCTGGTTTCTTGATGGAAGCCGAATTGGCGGCGCTTGGCAAAGCCTTGGATGATCCTCA
ACGACCTTTCGTTACGATCATTGGTGGCGCAAAAATTAGCGACAAAATCGGCGTGATCGAAAACTTGCTGGGCAAAGTCG
ATTCATTGCTGATTGGCGGCGGTATGGCCAATACCTTCTTGTTGGCGCAAAACAAGGCGGTTGGCGCTTCATTGGTCGAA
GCCGATAGCGTGGCCGAAGCAGGCCGTTTGATCGACGAAGCCCAAGGTCGTGGCGTGAAATTGTTGTTGCCCAGCGATGT
AGTGGTGGCCGATGCCTTCAGTGCCGAAGCCAATACCCAAGTGCTGAGCATCGACGATGTGCCCGATGGCTGGCGAATTC
TCGATATTGGGCCAGAAACCCGCCAAACCTACTCGCAAGTGGTTAGCGAAGCCAAAACCGTGATTTGGAACGGCCCAATG
GGTGTGTTTGAGCTAGAGCCATTTGCTGCTGGCACTCGCGCCGTTGCTCAAGCCTTGGCTGATAGCAGCGCAATCACGAT
TATCGGTGGTGGCGATTCGGTTGCAGCGATTGAGCAAATGGGCTTAGCTGAAAAGATGAGCCACATCTCAACTGGCGGTG
GCGCGAGCCTCGAATTGCTCGAAGGCAAAGTGCTGCCAGGCATCGCCGCGCTCAACGATAAATAA

Upstream 100 bases:

>100_bases
TCGTTTATTATGGGGAGCATAGGCTATGCGGTTCTTGGTTCGTAGCACTGATAGCGCCCAGATAGCCTATCGCCTATAGC
CTTGTACAAGGAGATCGCTT

Downstream 100 bases:

>100_bases
CTCGCTCGCTTACTCAAGTACGCAGCCCTACAGAACGCTTGATTCTGTGGGGCTTTTTATTAGCAACCTTTGAAGAGCAA
GCATTCTGTGAGTTGAGAGG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNKQTIRDVAWSGKRALVRVDFNVPLDQARKVTDDTRIRAALPTINYLLEQGASVILMSHLGRPKKKVAEEFRLKPVADH
LQSLLDAPVNYIQTTTGAEAEAAAQALQAGQVLLLENTRFDPREESNDPAMAEELAKLGDIYVNDAFGAAHRAHASTEGL
AKFLPAVAGFLMEAELAALGKALDDPQRPFVTIIGGAKISDKIGVIENLLGKVDSLLIGGGMANTFLLAQNKAVGASLVE
ADSVAEAGRLIDEAQGRGVKLLLPSDVVVADAFSAEANTQVLSIDDVPDGWRILDIGPETRQTYSQVVSEAKTVIWNGPM
GVFELEPFAAGTRAVAQALADSSAITIIGGGDSVAAIEQMGLAEKMSHISTGGGASLELLEGKVLPGIAALNDK

Sequences:

>Translated_394_residues
MNKQTIRDVAWSGKRALVRVDFNVPLDQARKVTDDTRIRAALPTINYLLEQGASVILMSHLGRPKKKVAEEFRLKPVADH
LQSLLDAPVNYIQTTTGAEAEAAAQALQAGQVLLLENTRFDPREESNDPAMAEELAKLGDIYVNDAFGAAHRAHASTEGL
AKFLPAVAGFLMEAELAALGKALDDPQRPFVTIIGGAKISDKIGVIENLLGKVDSLLIGGGMANTFLLAQNKAVGASLVE
ADSVAEAGRLIDEAQGRGVKLLLPSDVVVADAFSAEANTQVLSIDDVPDGWRILDIGPETRQTYSQVVSEAKTVIWNGPM
GVFELEPFAAGTRAVAQALADSSAITIIGGGDSVAAIEQMGLAEKMSHISTGGGASLELLEGKVLPGIAALNDK
>Mature_394_residues
MNKQTIRDVAWSGKRALVRVDFNVPLDQARKVTDDTRIRAALPTINYLLEQGASVILMSHLGRPKKKVAEEFRLKPVADH
LQSLLDAPVNYIQTTTGAEAEAAAQALQAGQVLLLENTRFDPREESNDPAMAEELAKLGDIYVNDAFGAAHRAHASTEGL
AKFLPAVAGFLMEAELAALGKALDDPQRPFVTIIGGAKISDKIGVIENLLGKVDSLLIGGGMANTFLLAQNKAVGASLVE
ADSVAEAGRLIDEAQGRGVKLLLPSDVVVADAFSAEANTQVLSIDDVPDGWRILDIGPETRQTYSQVVSEAKTVIWNGPM
GVFELEPFAAGTRAVAQALADSSAITIIGGGDSVAAIEQMGLAEKMSHISTGGGASLELLEGKVLPGIAALNDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=46.5060240963855, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI31543397, Length=414, Percent_Identity=44.2028985507246, Blast_Score=337, Evalue=1e-92,
Organism=Escherichia coli, GI1789294, Length=400, Percent_Identity=47, Blast_Score=338, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=46.9733656174334, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319857, Length=414, Percent_Identity=47.5845410628019, Blast_Score=369, Evalue=1e-103,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=44.6601941747573, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=42.4096385542169, Blast_Score=308, Evalue=5e-84,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_HERA2 (A9B312)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001547603.1
- ProteinModelPortal:   A9B312
- SMR:   A9B312
- GeneID:   5736691
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_4845
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   HAUR316274:HAUR_4845-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41534; Mature: 41534

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 37-37 BINDING 119-119 BINDING 152-152 BINDING 202-202 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQTIRDVAWSGKRALVRVDFNVPLDQARKVTDDTRIRAALPTINYLLEQGASVILMSH
CCCHHHHHHHCCCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEHHH
LGRPKKKVAEEFRLKPVADHLQSLLDAPVNYIQTTTGAEAEAAAQALQAGQVLLLENTRF
CCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCC
DPREESNDPAMAEELAKLGDIYVNDAFGAAHRAHASTEGLAKFLPAVAGFLMEAELAALG
CCCCCCCCCHHHHHHHHHCCEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KALDDPQRPFVTIIGGAKISDKIGVIENLLGKVDSLLIGGGMANTFLLAQNKAVGASLVE
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCHHEEEEECCCCCCHHHHH
ADSVAEAGRLIDEAQGRGVKLLLPSDVVVADAFSAEANTQVLSIDDVPDGWRILDIGPET
HHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCEEEEECCCCCCCEEEECCCHH
RQTYSQVVSEAKTVIWNGPMGVFELEPFAAGTRAVAQALADSSAITIIGGGDSVAAIEQM
HHHHHHHHHHHCEEEECCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
GLAEKMSHISTGGGASLELLEGKVLPGIAALNDK
HHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNKQTIRDVAWSGKRALVRVDFNVPLDQARKVTDDTRIRAALPTINYLLEQGASVILMSH
CCCHHHHHHHCCCCEEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEHHH
LGRPKKKVAEEFRLKPVADHLQSLLDAPVNYIQTTTGAEAEAAAQALQAGQVLLLENTRF
CCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCC
DPREESNDPAMAEELAKLGDIYVNDAFGAAHRAHASTEGLAKFLPAVAGFLMEAELAALG
CCCCCCCCCHHHHHHHHHCCEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KALDDPQRPFVTIIGGAKISDKIGVIENLLGKVDSLLIGGGMANTFLLAQNKAVGASLVE
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCHHEEEEECCCCCCHHHHH
ADSVAEAGRLIDEAQGRGVKLLLPSDVVVADAFSAEANTQVLSIDDVPDGWRILDIGPET
HHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCEEEEECCCCCCCEEEECCCHH
RQTYSQVVSEAKTVIWNGPMGVFELEPFAAGTRAVAQALADSSAITIIGGGDSVAAIEQM
HHHHHHHHHHHCEEEECCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
GLAEKMSHISTGGGASLELLEGKVLPGIAALNDK
HHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA