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The map label for this gene is yoaE [H]

Identifier: 159901185

GI number: 159901185

Start: 5969255

End: 5971327

Strand: Direct

Name: yoaE [H]

Synonym: Haur_4673

Alternate gene names: 159901185

Gene position: 5969255-5971327 (Clockwise)

Preceding gene: 159901182

Following gene: 159901186

Centisome position: 94.05

GC content: 52.29

Gene sequence:

>2073_bases
ATGACAACGATTGCCCTTGATCAGCAGATTATTCATGCGGTTTGCCCCCACGATTGCCCCGATACCTGCTCGATGCTTGT
AACCGTGCAGGATGGTAAGGCGATTAAGATTCAGGGCAACCCTGAGCACCCTATAACCAATGGTTTTTTGTGCACCAAAG
TCTCGCGCTATGTTGAGCGTACCTACCACCAAGGCCGGTTGTTGTATCCGGCCAAGCGGGTTGGTCCCAAGGGTGCAGGC
CAATTCGAACGGATCTCGTGGGACGAAGCCTTAACAACCATCGCCGAATGTTTGCAAGCAATCAGCACTAGCCATGGCCC
GCAATCAATTTTGCCCTACTCCTATGCTGGCACTATGGGTTTGCTGCATTATGGCTCGATGGATCGACGCTTTTTCCATC
GTTTGGGCGCTTCATTGCTCGACCGCACGATTTGTGCCAGCGCTGGGGCAACTGGCTATCGCTATACGGTTGGCGCGAGT
GTTGGCACTGACCCCGAGGCCTATGTTGATGCCAAATTAATTATTTTGTGGGGAACCAACACACTCACGTCGAATGTGCA
CTTGTGGACCTTCATCAAGGCGGCACGTAAAGCTGGCGCACGGGTGATTGCGATCGATCCTTGGCGTTCGCGCACTGCCG
CCCAATGCGATTGGCATATTGCACCTTTGCCAGGCACTGATGCTGCCTTGGCGCTGGGCATGATGCATATTATTTTTCGT
GATGGCTTGCAGGATCAAGCCTATCTTGATGAATATTGTCTTGGTGGCGAACAATTGCGCCAGCGCGTGCAGGAATATCC
GCCAGAGCTTGTGGCCGAATTGACTGGTTTGGATGTCGCAACGATTGAGCAATTGGCCCACGAATATGCTACGACTGCAC
CCGCTGCGATTCGGATTAATTATGGCTTGCAACGCCACGCTGGCGGCGGCATGGCCGTGCGTACTTTGGCTTGTTTGCCT
GCGGTAATCGGTTCGTGGCGTGAGCGAGCAGGCGGCATTTTGCTTTCCACTAGCGGCACATTTCCAATTAATAATGCGGC
CTTGGAGCGCCCTGATCTGATTCCTGCTGGCACTCGCATGATCAATATGAATCAGCTTGGTGATGCCCTGACCGAATTAA
ACGACCCGCCAGTGCAAGCTTTGTTTGTCTACAATTCTAATCCGGCGAGCGTGGCCCCCGATTTGGAAGCAGTCAAGCGT
GGCTTGGAGCGCGAAGATTTGTTCACGGTGGTGCACGAGCAGTTTTACACCGACACGACGCGCTACGCTGATATTATTTT
GCCTGCAACCACCCAACTAGAGCATGTAGATTTACATAAAGCCTATGGTCACTGGTATTTAGCTTGGAGCAACCAAGCGA
TTGAGCCGCTAGGCGAGGCGATTCCGAATACCGAGTTGTTTCGGCGCTTGGCGGCCAAAATGGGCTTTAGCGAGCCATGT
TTTAGCGATGATGATCAAACGATCGCTCGCCAAGCACTCAATTCAAATCATCCAGCCTTGGCAGGCATTACCCTCGAACG
TTTGCAAGAAGCGGGTTGGCTGCGTTTAAATGTACCAAGCAATTGGGCACCGTTTGCTGAGGGCAATTTTCTCACACCAT
CGGGCAAGTGCGAACTCTATTCTGAACGGATGTTGGCTGATGGTTTTGATCCATTGCCGACCTATACCGCGCCTTACGAA
AGCCCAGTGACTAACCCACGTTTGGCTGAGCGCTATCCATTGACCCTAATTTCGCCGCCAGCCCATCACTTTTTAAATAG
CACCTTTGGCAATATTTTGCAGCGCTACGAAAGTGGCCCGACCCTAGAAATTAATCCACTTGATGCGATTGAGCGCAATA
TCGACGATGGCGATCAAGTATTGATTCGCAACGATCGTGGCGGTTTTGTGGCAACAGCAGTGGTGACTGATCGGGTGCGG
CAAGCTGTGGTGGTTGCGCCATCAATTTGGTGGTCGGAGCTAACCGTCGATGGCCGCAACGTCAACCATACCACACCCCA
AGCGCTTTCGGATATGGGCGGCGGTGCAACGTTCTACGATAATTTGGTTGAGGTTGAGCGTATTGTCAGCTAA

Upstream 100 bases:

>100_bases
TCCATAACATGCAGCTGATAATTCGTTGAAAAAAACGAAAAGTTGTATAGTAGTTGGCAACAACTGCGAAGGAGGCGGGT
TTTTCAGCCCGATTGATGCC

Downstream 100 bases:

>100_bases
GCTATGTGGTTGCTGGTATATTCGTGGTTAGCGTAACGATACCCAAACGTTGAGGAGTGTGTATGAGTTTTTTGCGTAAG
CTTTTTGGCAGTAGCAGCCA

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MTTIALDQQIIHAVCPHDCPDTCSMLVTVQDGKAIKIQGNPEHPITNGFLCTKVSRYVERTYHQGRLLYPAKRVGPKGAG
QFERISWDEALTTIAECLQAISTSHGPQSILPYSYAGTMGLLHYGSMDRRFFHRLGASLLDRTICASAGATGYRYTVGAS
VGTDPEAYVDAKLIILWGTNTLTSNVHLWTFIKAARKAGARVIAIDPWRSRTAAQCDWHIAPLPGTDAALALGMMHIIFR
DGLQDQAYLDEYCLGGEQLRQRVQEYPPELVAELTGLDVATIEQLAHEYATTAPAAIRINYGLQRHAGGGMAVRTLACLP
AVIGSWRERAGGILLSTSGTFPINNAALERPDLIPAGTRMINMNQLGDALTELNDPPVQALFVYNSNPASVAPDLEAVKR
GLEREDLFTVVHEQFYTDTTRYADIILPATTQLEHVDLHKAYGHWYLAWSNQAIEPLGEAIPNTELFRRLAAKMGFSEPC
FSDDDQTIARQALNSNHPALAGITLERLQEAGWLRLNVPSNWAPFAEGNFLTPSGKCELYSERMLADGFDPLPTYTAPYE
SPVTNPRLAERYPLTLISPPAHHFLNSTFGNILQRYESGPTLEINPLDAIERNIDDGDQVLIRNDRGGFVATAVVTDRVR
QAVVVAPSIWWSELTVDGRNVNHTTPQALSDMGGGATFYDNLVEVERIVS

Sequences:

>Translated_690_residues
MTTIALDQQIIHAVCPHDCPDTCSMLVTVQDGKAIKIQGNPEHPITNGFLCTKVSRYVERTYHQGRLLYPAKRVGPKGAG
QFERISWDEALTTIAECLQAISTSHGPQSILPYSYAGTMGLLHYGSMDRRFFHRLGASLLDRTICASAGATGYRYTVGAS
VGTDPEAYVDAKLIILWGTNTLTSNVHLWTFIKAARKAGARVIAIDPWRSRTAAQCDWHIAPLPGTDAALALGMMHIIFR
DGLQDQAYLDEYCLGGEQLRQRVQEYPPELVAELTGLDVATIEQLAHEYATTAPAAIRINYGLQRHAGGGMAVRTLACLP
AVIGSWRERAGGILLSTSGTFPINNAALERPDLIPAGTRMINMNQLGDALTELNDPPVQALFVYNSNPASVAPDLEAVKR
GLEREDLFTVVHEQFYTDTTRYADIILPATTQLEHVDLHKAYGHWYLAWSNQAIEPLGEAIPNTELFRRLAAKMGFSEPC
FSDDDQTIARQALNSNHPALAGITLERLQEAGWLRLNVPSNWAPFAEGNFLTPSGKCELYSERMLADGFDPLPTYTAPYE
SPVTNPRLAERYPLTLISPPAHHFLNSTFGNILQRYESGPTLEINPLDAIERNIDDGDQVLIRNDRGGFVATAVVTDRVR
QAVVVAPSIWWSELTVDGRNVNHTTPQALSDMGGGATFYDNLVEVERIVS
>Mature_689_residues
TTIALDQQIIHAVCPHDCPDTCSMLVTVQDGKAIKIQGNPEHPITNGFLCTKVSRYVERTYHQGRLLYPAKRVGPKGAGQ
FERISWDEALTTIAECLQAISTSHGPQSILPYSYAGTMGLLHYGSMDRRFFHRLGASLLDRTICASAGATGYRYTVGASV
GTDPEAYVDAKLIILWGTNTLTSNVHLWTFIKAARKAGARVIAIDPWRSRTAAQCDWHIAPLPGTDAALALGMMHIIFRD
GLQDQAYLDEYCLGGEQLRQRVQEYPPELVAELTGLDVATIEQLAHEYATTAPAAIRINYGLQRHAGGGMAVRTLACLPA
VIGSWRERAGGILLSTSGTFPINNAALERPDLIPAGTRMINMNQLGDALTELNDPPVQALFVYNSNPASVAPDLEAVKRG
LEREDLFTVVHEQFYTDTTRYADIILPATTQLEHVDLHKAYGHWYLAWSNQAIEPLGEAIPNTELFRRLAAKMGFSEPCF
SDDDQTIARQALNSNHPALAGITLERLQEAGWLRLNVPSNWAPFAEGNFLTPSGKCELYSERMLADGFDPLPTYTAPYES
PVTNPRLAERYPLTLISPPAHHFLNSTFGNILQRYESGPTLEINPLDAIERNIDDGDQVLIRNDRGGFVATAVVTDRVRQ
AVVVAPSIWWSELTVDGRNVNHTTPQALSDMGGGATFYDNLVEVERIVS

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=767, Percent_Identity=29.2046936114733, Blast_Score=222, Evalue=6e-59,
Organism=Escherichia coli, GI1787870, Length=770, Percent_Identity=27.012987012987, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI171474008, Length=774, Percent_Identity=26.6149870801034, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI3868721, Length=683, Percent_Identity=25.4758418740849, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI145693196, Length=688, Percent_Identity=27.1802325581395, Blast_Score=153, Evalue=4e-38,
Organism=Escherichia coli, GI87081994, Length=733, Percent_Identity=26.0572987721692, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1787231, Length=689, Percent_Identity=25.3991291727141, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1788534, Length=802, Percent_Identity=20.6982543640898, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI3868720, Length=393, Percent_Identity=23.1552162849873, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI3868719, Length=406, Percent_Identity=22.9064039408867, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787741, Length=184, Percent_Identity=29.8913043478261, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787477, Length=279, Percent_Identity=24.0143369175627, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 75906; Mature: 75775

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIALDQQIIHAVCPHDCPDTCSMLVTVQDGKAIKIQGNPEHPITNGFLCTKVSRYVER
CCEEHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHH
TYHQGRLLYPAKRVGPKGAGQFERISWDEALTTIAECLQAISTSHGPQSILPYSYAGTMG
HHHCCEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCHHH
LLHYGSMDRRFFHRLGASLLDRTICASAGATGYRYTVGASVGTDPEAYVDAKLIILWGTN
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEECEEEEEEECCC
TLTSNVHLWTFIKAARKAGARVIAIDPWRSRTAAQCDWHIAPLPGTDAALALGMMHIIFR
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
DGLQDQAYLDEYCLGGEQLRQRVQEYPPELVAELTGLDVATIEQLAHEYATTAPAAIRIN
CCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEE
YGLQRHAGGGMAVRTLACLPAVIGSWRERAGGILLSTSGTFPINNAALERPDLIPAGTRM
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCEE
INMNQLGDALTELNDPPVQALFVYNSNPASVAPDLEAVKRGLEREDLFTVVHEQFYTDTT
EEHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCH
RYADIILPATTQLEHVDLHKAYGHWYLAWSNQAIEPLGEAIPNTELFRRLAAKMGFSEPC
HHEEEEECCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHCCCCCCC
FSDDDQTIARQALNSNHPALAGITLERLQEAGWLRLNVPSNWAPFAEGNFLTPSGKCELY
CCCCHHHHHHHHHCCCCCEEEHHHHHHHHHCCEEEEECCCCCCCCCCCCEECCCCCCHHH
SERMLADGFDPLPTYTAPYESPVTNPRLAERYPLTLISPPAHHFLNSTFGNILQRYESGP
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCC
TLEINPLDAIERNIDDGDQVLIRNDRGGFVATAVVTDRVRQAVVVAPSIWWSELTVDGRN
EEEECCHHHHHCCCCCCCEEEEEECCCCEEEEHHHHHHHHHEEEEECCCCEEHEEECCCC
VNHTTPQALSDMGGGATFYDNLVEVERIVS
CCCCCHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTIALDQQIIHAVCPHDCPDTCSMLVTVQDGKAIKIQGNPEHPITNGFLCTKVSRYVER
CEEHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHH
TYHQGRLLYPAKRVGPKGAGQFERISWDEALTTIAECLQAISTSHGPQSILPYSYAGTMG
HHHCCEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCHHH
LLHYGSMDRRFFHRLGASLLDRTICASAGATGYRYTVGASVGTDPEAYVDAKLIILWGTN
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEECEEEEEEECCC
TLTSNVHLWTFIKAARKAGARVIAIDPWRSRTAAQCDWHIAPLPGTDAALALGMMHIIFR
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
DGLQDQAYLDEYCLGGEQLRQRVQEYPPELVAELTGLDVATIEQLAHEYATTAPAAIRIN
CCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEE
YGLQRHAGGGMAVRTLACLPAVIGSWRERAGGILLSTSGTFPINNAALERPDLIPAGTRM
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCEE
INMNQLGDALTELNDPPVQALFVYNSNPASVAPDLEAVKRGLEREDLFTVVHEQFYTDTT
EEHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCH
RYADIILPATTQLEHVDLHKAYGHWYLAWSNQAIEPLGEAIPNTELFRRLAAKMGFSEPC
HHEEEEECCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHCCCCCCC
FSDDDQTIARQALNSNHPALAGITLERLQEAGWLRLNVPSNWAPFAEGNFLTPSGKCELY
CCCCHHHHHHHHHCCCCCEEEHHHHHHHHHCCEEEEECCCCCCCCCCCCEECCCCCCHHH
SERMLADGFDPLPTYTAPYESPVTNPRLAERYPLTLISPPAHHFLNSTFGNILQRYESGP
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCC
TLEINPLDAIERNIDDGDQVLIRNDRGGFVATAVVTDRVRQAVVVAPSIWWSELTVDGRN
EEEECCHHHHHCCCCCCCEEEEEECCCCEEEEHHHHHHHHHEEEEECCCCEEHEEECCCC
VNHTTPQALSDMGGGATFYDNLVEVERIVS
CCCCCHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]