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The map label for this gene is yfkN [H]

Identifier: 159900876

GI number: 159900876

Start: 5573524

End: 5575425

Strand: Direct

Name: yfkN [H]

Synonym: Haur_4363

Alternate gene names: 159900876

Gene position: 5573524-5575425 (Clockwise)

Preceding gene: 159900875

Following gene: 159900877

Centisome position: 87.82

GC content: 50.26

Gene sequence:

>1902_bases
ATGCGTTTGCTACGTGCGTTCGCGTTGCTGCTATTGCTGGCAGTTTTGGCTCCACTCGGCTTGCAGAGTGGGGTTGTAAG
TGCCCAAAATGAGCAACGCACGGTCACTATTTTGGAAACGAGCGATATCCACGGCAATTTGATGGCTTGGGATTATTACG
CCAACAAGCCTGCCGAATGGGGTATGACCAAGGTTGCAAGCTTGATCAAACAAGAACGGGCGATCGATCCCAATCTCTTG
TTGGTCGATAATGGTGATACGATTCAAGGTACGCCCTTGACCTACTACTACAACGTGATCGACCAAAATGCCGCGCATCC
AATGGCAGCGGTATTTAATGCGCTCAAATATGATGTTTCATCGTTGGGCAACCACGAATTTAATTATGGGATGGATGTGC
TGAATCGCTATATCAGCCAAGCTCAATACCCAGTTATGAGCGCCAATGTGCGCAAAAGCGATGGTAGCGAAGCCTTCAAG
CCCTACATTATTAAAGATGTGAATGGCGTGAAAGTAGGCTTTTTGGCCTTGACCACGCCAACTGTGCCAACCTGGGAAAA
ACCCGCCAACATCGCGGGCCTGCAATTTGCCGATCCGGTAGAAGTTGCCAAGCAATATGTACCGCAAATTCGGGCTGAAG
GTGCGCATATTGTGGTTTTGCTGCAACACACGGGCTGGGAAAAACAGCCTGCCGAAGCGACCAAACCCGAAGCATGGCTA
ACCGACCCCAGCACTTGGCGCGATACTGGCTCGTTGCCAGGCGAAAATGTGTCGATCAAACTTGCCCAAGAAGTGCCTGG
CGTTGACGTGATTTTGACTGGTCACTCACACTTGAGCGTGCCCAAGGCGATTATCAACAATGTGTTATTGATCGAGCCAT
CGTATTGGGGCCGCGCTTTGGGCAAAGTGACGATTACGGTTGAGAAAAATGGCGATAGCTGGAATGTGGTTAACAAAGAT
TCAACCAACATTTCAGTCACCAATGTTGCCGAAGATCAAGAGATTAAAGCACTGGTACAACCATATCACGACCAAACCTT
GAGCTATATTAGCCAACCAGTTGGTACGGCTAGCGCCGAATTTGCTGGCGGCCCCAAGGCGCGTTATCGTGATAGCGCTT
TGGCCGATTTGATCAACAATGTGCAAAAGCAAGCCGCTGCTGATGCTGGCTACCCCGTTGATCTCTCGTTGGCAGCGATT
TTCACCGATGGCGGCATGATTCCGGCGGGCCAAATTACCCTGCGCGATGCCTACAGCATCTATATTTACGATAATACGCT
GTATGTGATGGAAATTAATGGTGATATTCTGCGCCGTGCTTTGGAGCGTAACGCCGAATATTTCCGCCAGCTTGATCCCA
ATGCCTTGCCCAGCGATCCCAAAGCGGTAGTCAACGATAATGCCCGCGATTACAACTGGGATTTATACACCGATATCGAC
TATAGCTACGATTTGACCAAGCCAGCCGGCCAGCGTGTGACCAAATTGCAATTGAATGGGGTTGATATTACACCTGAACA
AACCCTGCGCATCGCGATCAACAATTACCGAGCTGGCGGCGGCGGTGGCTTTGCCATGTTCCGTGAAGGCAAAATTGTCT
ATCAATCGACCAGCGAAATTCGCGATTTGATCGCTGAGTCAGTCAAAAATGCTGGCACAATTGATCCGACGGTGGTGAAT
AAGGTTAATTTTACCCTTGTGCCAGATTTATATGCCCACTATTTTGGTGCTGCCAGCCAGCCGACTGCTACGCCAGTGCC
AGCCCAACCAACTGCCACTCCAGCGCCAGGTGTGCCAATTACCTTGCCTGATACCAGTGGTAACCAACCAAGCTATGCCT
GGGTTTGGGCGGCTGTCGCCATGGCCTTACTCGCTTTAGGTTTGGTTGTGCGCCGCAATTAA

Upstream 100 bases:

>100_bases
GAACTTAATATTTTTGACCGAATCTACGGAGCGGCGTATTATAGCACTATATGCTCGGATCGAACATCCAGCATATTAGT
GTTTTAGGAGGATAGAGTTT

Downstream 100 bases:

>100_bases
AGGCAAAGGGCAGAAGGCAAAATTCAAAAGCAGGGGCTAAGGTAGGAGATTAAATTTAACCGCTAAGAACGCGAAGGGCA
CGAATATTTTGTTGTTGGGA

Product: cell wall anchor domain-containing protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MRLLRAFALLLLLAVLAPLGLQSGVVSAQNEQRTVTILETSDIHGNLMAWDYYANKPAEWGMTKVASLIKQERAIDPNLL
LVDNGDTIQGTPLTYYYNVIDQNAAHPMAAVFNALKYDVSSLGNHEFNYGMDVLNRYISQAQYPVMSANVRKSDGSEAFK
PYIIKDVNGVKVGFLALTTPTVPTWEKPANIAGLQFADPVEVAKQYVPQIRAEGAHIVVLLQHTGWEKQPAEATKPEAWL
TDPSTWRDTGSLPGENVSIKLAQEVPGVDVILTGHSHLSVPKAIINNVLLIEPSYWGRALGKVTITVEKNGDSWNVVNKD
STNISVTNVAEDQEIKALVQPYHDQTLSYISQPVGTASAEFAGGPKARYRDSALADLINNVQKQAAADAGYPVDLSLAAI
FTDGGMIPAGQITLRDAYSIYIYDNTLYVMEINGDILRRALERNAEYFRQLDPNALPSDPKAVVNDNARDYNWDLYTDID
YSYDLTKPAGQRVTKLQLNGVDITPEQTLRIAINNYRAGGGGGFAMFREGKIVYQSTSEIRDLIAESVKNAGTIDPTVVN
KVNFTLVPDLYAHYFGAASQPTATPVPAQPTATPAPGVPITLPDTSGNQPSYAWVWAAVAMALLALGLVVRRN

Sequences:

>Translated_633_residues
MRLLRAFALLLLLAVLAPLGLQSGVVSAQNEQRTVTILETSDIHGNLMAWDYYANKPAEWGMTKVASLIKQERAIDPNLL
LVDNGDTIQGTPLTYYYNVIDQNAAHPMAAVFNALKYDVSSLGNHEFNYGMDVLNRYISQAQYPVMSANVRKSDGSEAFK
PYIIKDVNGVKVGFLALTTPTVPTWEKPANIAGLQFADPVEVAKQYVPQIRAEGAHIVVLLQHTGWEKQPAEATKPEAWL
TDPSTWRDTGSLPGENVSIKLAQEVPGVDVILTGHSHLSVPKAIINNVLLIEPSYWGRALGKVTITVEKNGDSWNVVNKD
STNISVTNVAEDQEIKALVQPYHDQTLSYISQPVGTASAEFAGGPKARYRDSALADLINNVQKQAAADAGYPVDLSLAAI
FTDGGMIPAGQITLRDAYSIYIYDNTLYVMEINGDILRRALERNAEYFRQLDPNALPSDPKAVVNDNARDYNWDLYTDID
YSYDLTKPAGQRVTKLQLNGVDITPEQTLRIAINNYRAGGGGGFAMFREGKIVYQSTSEIRDLIAESVKNAGTIDPTVVN
KVNFTLVPDLYAHYFGAASQPTATPVPAQPTATPAPGVPITLPDTSGNQPSYAWVWAAVAMALLALGLVVRRN
>Mature_633_residues
MRLLRAFALLLLLAVLAPLGLQSGVVSAQNEQRTVTILETSDIHGNLMAWDYYANKPAEWGMTKVASLIKQERAIDPNLL
LVDNGDTIQGTPLTYYYNVIDQNAAHPMAAVFNALKYDVSSLGNHEFNYGMDVLNRYISQAQYPVMSANVRKSDGSEAFK
PYIIKDVNGVKVGFLALTTPTVPTWEKPANIAGLQFADPVEVAKQYVPQIRAEGAHIVVLLQHTGWEKQPAEATKPEAWL
TDPSTWRDTGSLPGENVSIKLAQEVPGVDVILTGHSHLSVPKAIINNVLLIEPSYWGRALGKVTITVEKNGDSWNVVNKD
STNISVTNVAEDQEIKALVQPYHDQTLSYISQPVGTASAEFAGGPKARYRDSALADLINNVQKQAAADAGYPVDLSLAAI
FTDGGMIPAGQITLRDAYSIYIYDNTLYVMEINGDILRRALERNAEYFRQLDPNALPSDPKAVVNDNARDYNWDLYTDID
YSYDLTKPAGQRVTKLQLNGVDITPEQTLRIAINNYRAGGGGGFAMFREGKIVYQSTSEIRDLIAESVKNAGTIDPTVVN
KVNFTLVPDLYAHYFGAASQPTATPVPAQPTATPAPGVPITLPDTSGNQPSYAWVWAAVAMALLALGLVVRRN

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=569, Percent_Identity=23.3743409490334, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1790658, Length=602, Percent_Identity=32.7242524916944, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1786687, Length=572, Percent_Identity=21.6783216783217, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19921980, Length=518, Percent_Identity=21.6216216216216, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24652512, Length=518, Percent_Identity=21.6216216216216, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI161076508, Length=518, Percent_Identity=21.6216216216216, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922446, Length=547, Percent_Identity=21.5722120658135, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24654424, Length=547, Percent_Identity=21.5722120658135, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24641187, Length=558, Percent_Identity=20.7885304659498, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922444, Length=436, Percent_Identity=22.4770642201835, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI221329836, Length=541, Percent_Identity=20.1478743068392, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573524, Length=427, Percent_Identity=21.0772833723653, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 69077; Mature: 69077

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLRAFALLLLLAVLAPLGLQSGVVSAQNEQRTVTILETSDIHGNLMAWDYYANKPAEW
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCC
GMTKVASLIKQERAIDPNLLLVDNGDTIQGTPLTYYYNVIDQNAAHPMAAVFNALKYDVS
CHHHHHHHHHHHHCCCCCEEEEECCCEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
SLGNHEFNYGMDVLNRYISQAQYPVMSANVRKSDGSEAFKPYIIKDVNGVKVGFLALTTP
HCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCEEEEEEEECC
TVPTWEKPANIAGLQFADPVEVAKQYVPQIRAEGAHIVVLLQHTGWEKQPAEATKPEAWL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCC
TDPSTWRDTGSLPGENVSIKLAQEVPGVDVILTGHSHLSVPKAIINNVLLIEPSYWGRAL
CCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHCCEEEECCCHHCCCC
GKVTITVEKNGDSWNVVNKDSTNISVTNVAEDQEIKALVQPYHDQTLSYISQPVGTASAE
CEEEEEEEECCCCEEEECCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCC
FAGGPKARYRDSALADLINNVQKQAAADAGYPVDLSLAAIFTDGGMIPAGQITLRDAYSI
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCEEEEEEEEEE
YIYDNTLYVMEINGDILRRALERNAEYFRQLDPNALPSDPKAVVNDNARDYNWDLYTDID
EEEECEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCEEEEECC
YSYDLTKPAGQRVTKLQLNGVDITPEQTLRIAINNYRAGGGGGFAMFREGKIVYQSTSEI
CCEECCCCCCCCEEEEEECCEECCCCCEEEEEEECEECCCCCCEEEEECCEEEEECHHHH
RDLIAESVKNAGTIDPTVVNKVNFTLVPDLYAHYFGAASQPTATPVPAQPTATPAPGVPI
HHHHHHHHHCCCCCCCCEECEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
TLPDTSGNQPSYAWVWAAVAMALLALGLVVRRN
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLLRAFALLLLLAVLAPLGLQSGVVSAQNEQRTVTILETSDIHGNLMAWDYYANKPAEW
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCC
GMTKVASLIKQERAIDPNLLLVDNGDTIQGTPLTYYYNVIDQNAAHPMAAVFNALKYDVS
CHHHHHHHHHHHHCCCCCEEEEECCCEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
SLGNHEFNYGMDVLNRYISQAQYPVMSANVRKSDGSEAFKPYIIKDVNGVKVGFLALTTP
HCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCEEEEEEEECC
TVPTWEKPANIAGLQFADPVEVAKQYVPQIRAEGAHIVVLLQHTGWEKQPAEATKPEAWL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCC
TDPSTWRDTGSLPGENVSIKLAQEVPGVDVILTGHSHLSVPKAIINNVLLIEPSYWGRAL
CCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHCCEEEECCCHHCCCC
GKVTITVEKNGDSWNVVNKDSTNISVTNVAEDQEIKALVQPYHDQTLSYISQPVGTASAE
CEEEEEEEECCCCEEEECCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCC
FAGGPKARYRDSALADLINNVQKQAAADAGYPVDLSLAAIFTDGGMIPAGQITLRDAYSI
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCEEEEEEEEEE
YIYDNTLYVMEINGDILRRALERNAEYFRQLDPNALPSDPKAVVNDNARDYNWDLYTDID
EEEECEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCEEEEECC
YSYDLTKPAGQRVTKLQLNGVDITPEQTLRIAINNYRAGGGGGFAMFREGKIVYQSTSEI
CCEECCCCCCCCEEEEEECCEECCCCCEEEEEEECEECCCCCCEEEEECCEEEEECHHHH
RDLIAESVKNAGTIDPTVVNKVNFTLVPDLYAHYFGAASQPTATPVPAQPTATPAPGVPI
HHHHHHHHHCCCCCCCCEECEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
TLPDTSGNQPSYAWVWAAVAMALLALGLVVRRN
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]