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The map label for this gene is nth [H]

Identifier: 159900799

GI number: 159900799

Start: 5473315

End: 5473989

Strand: Direct

Name: nth [H]

Synonym: Haur_4286

Alternate gene names: 159900799

Gene position: 5473315-5473989 (Clockwise)

Preceding gene: 159900798

Following gene: 159900804

Centisome position: 86.24

GC content: 52.3

Gene sequence:

>675_bases
ATGCAAGATCTCCAAGCTAAAGCCGATCTGGTTTATCGCGAGTTAGTCGCCTTGCATGGATTTAACCAACTTGTGCCACG
CCGCGAACCAATGCACGAGCTAATTTCGACCATGCTTTCGCATCAAACCACTGAGGCCAACGAAGAGCGTGGCTACCAAA
ATCTCCGCGCAACCTTTCCAACCTGGGAAGCCATCTTGGCAGCACCAGTCGAGGCCGTCGCCGAAGCGATCAAGCCAGCC
AATTACGCTCCCGCCAAAGCCAACAATATTCAAGCTGCCTTGGCCAAAATTCTGGCTGAGCGCGGCGAAATTTCGATTGA
TTTTCTGGCTGAGCTATCGACCGAAGCAGCAATGGCCTGGCTGACTGGGTTGCGTGGCGTTGGCCCGAAAACCGCCTCTT
TAGTGCTGTTGTTTTGTTTTAGCAAGCCGATTTTGCCTGTTGATACCCATGTGCATCGAGTCAGCCAACGCTTAGGCTTG
GTCAAGGCCAAAACACCCACCGAAGCCCACGAAATTCTGTGGCAATTGCTGCCGCATGACGCTGAATGGCTGTTTAACTA
TCACGTTGCACTATTACGCCACGGCCAACGCATCTGCTTAGCCAAACGACCACGTTGCAACCAATGCCCACTGACCGCGC
AATGTTTGTGGTATCAAGCTAATCAGGCTGAGTAA

Upstream 100 bases:

>100_bases
TCCCGCCCAGCAGGCGAGGGGGAAGCACCAAACCAAACGCCCCTCGCTCGCCGCAGTAGGCGAGGGGTTGGGGGTGAGGG
ACATTGTTTGGAGCAGATGT

Downstream 100 bases:

>100_bases
CTTTGCGCCAACGGGCACTTGGCCCACGCCCAAGTGGTCTTTCGATCATTAAAAATCAATGATAAGAGGTAATAAAATCG
CAAATTATATATGTAAAAGT

Product: HhH-GPD family protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MQDLQAKADLVYRELVALHGFNQLVPRREPMHELISTMLSHQTTEANEERGYQNLRATFPTWEAILAAPVEAVAEAIKPA
NYAPAKANNIQAALAKILAERGEISIDFLAELSTEAAMAWLTGLRGVGPKTASLVLLFCFSKPILPVDTHVHRVSQRLGL
VKAKTPTEAHEILWQLLPHDAEWLFNYHVALLRHGQRICLAKRPRCNQCPLTAQCLWYQANQAE

Sequences:

>Translated_224_residues
MQDLQAKADLVYRELVALHGFNQLVPRREPMHELISTMLSHQTTEANEERGYQNLRATFPTWEAILAAPVEAVAEAIKPA
NYAPAKANNIQAALAKILAERGEISIDFLAELSTEAAMAWLTGLRGVGPKTASLVLLFCFSKPILPVDTHVHRVSQRLGL
VKAKTPTEAHEILWQLLPHDAEWLFNYHVALLRHGQRICLAKRPRCNQCPLTAQCLWYQANQAE
>Mature_224_residues
MQDLQAKADLVYRELVALHGFNQLVPRREPMHELISTMLSHQTTEANEERGYQNLRATFPTWEAILAAPVEAVAEAIKPA
NYAPAKANNIQAALAKILAERGEISIDFLAELSTEAAMAWLTGLRGVGPKTASLVLLFCFSKPILPVDTHVHRVSQRLGL
VKAKTPTEAHEILWQLLPHDAEWLFNYHVALLRHGQRICLAKRPRCNQCPLTAQCLWYQANQAE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=219, Percent_Identity=29.2237442922374, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787920, Length=192, Percent_Identity=32.8125, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17554540, Length=184, Percent_Identity=33.1521739130435, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25102; Mature: 25102

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLQAKADLVYRELVALHGFNQLVPRREPMHELISTMLSHQTTEANEERGYQNLRATFP
CCCHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
TWEAILAAPVEAVAEAIKPANYAPAKANNIQAALAKILAERGEISIDFLAELSTEAAMAW
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LTGLRGVGPKTASLVLLFCFSKPILPVDTHVHRVSQRLGLVKAKTPTEAHEILWQLLPHD
HHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
AEWLFNYHVALLRHGQRICLAKRPRCNQCPLTAQCLWYQANQAE
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHEECCCCCC
>Mature Secondary Structure
MQDLQAKADLVYRELVALHGFNQLVPRREPMHELISTMLSHQTTEANEERGYQNLRATFP
CCCHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
TWEAILAAPVEAVAEAIKPANYAPAKANNIQAALAKILAERGEISIDFLAELSTEAAMAW
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LTGLRGVGPKTASLVLLFCFSKPILPVDTHVHRVSQRLGLVKAKTPTEAHEILWQLLPHD
HHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
AEWLFNYHVALLRHGQRICLAKRPRCNQCPLTAQCLWYQANQAE
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]