Click here to switch to the map view.

The map label for this gene is vipB [H]

Identifier: 159900791

GI number: 159900791

Start: 5462649

End: 5463587

Strand: Direct

Name: vipB [H]

Synonym: Haur_4278

Alternate gene names: 159900791

Gene position: 5462649-5463587 (Clockwise)

Preceding gene: 159900790

Following gene: 159900792

Centisome position: 86.07

GC content: 51.76

Gene sequence:

>939_bases
ATGGCGCGATATTTAGTAACTGGTGGCGCAGGCTTTATTGGCTCGCATTTGGTTGATGCGCTCTTGCAACGCGGCGATGA
GGTGCGGGTTTTCGATAATTTTTCGACTGGCTATGAGCATAATCTGGCGCATTGTATTAATGATATTGAGTTGGTGCGCG
GCGATTTACGCGATGCCGAGGCTGTGAGCCAAGCGGTGGCTGGCTGCGAAGTAATTTTTCACGAAGGTGCGTTGCCCTCA
GTGCCACGCTCGGTCAGCGACCCGCTAACCACCGATGCGGTCAATACTGGCGGCTCGTTGCATGTGTTACAAGCGGCGCG
GCAGCATGGCGCACGGCGCGTCGTTTTTGCAGCGTCATCATCGGTCTATGGCGATACCCCAATTTTGCCCAAAGTCGAAA
CTATGGCCATGAGCCCCAAATCGCCGTATGCAGTCAGCAAAATGGCCGCCGAAAGCTATTTGAAGGTTTTTCATCATGTG
TATGGCTTAGAAACGGTTGGCTTGCGCTATTTCAACGTTTTTGGGCCGCGCCAAGACCCAACTTCGCAATATTCGGGCGT
AATTGCTCGTTTTATGACCTTGGCCTTACAAGGCGAACCTTACACCATGAATGGCACTGGCAATCAATCACGCGACTTTA
CCTACGTTGCGAATGTGGTGCAGGCCAATTTGCTGGCGGCAAGCGTGCCTGCTGCGGCTGGCCATGTGTTTAACATTGCT
TGTGGCTTGCGCATTAGCCTTAATGATGTGGTTGCGATGTTGAACAAACTAGTTGGTAAAGAACTACCGATTATTTACAG
TCCAGCCCGTACTGGCGATGTTGAGCATTCGTTGGCCGATATTAGTGCTGCTCGCCAAATCTTGGGTTTTGAGCCAAGCG
TCGATATTGAAACTGGCATCGCCCGCACACTGGATTGGTATCGCACTCAGGGAGCCTAA

Upstream 100 bases:

>100_bases
CATTTAGCAGCATCACCACGGTGATTCAGCGCTTCCCACGGCCAAACGATAGCAATAACCCAATTGCGATTTTTGAACTT
AACTAAAAAGGAATAGCTTT

Downstream 100 bases:

>100_bases
ATTCAATGCGCATGCTCTACTTCACCACTGCCTACAACGCTGCCTTGCTTGATCGGGTGCATGAAGAATTTTTGTTGCGC
TGGCAGGCGCTTGGCCATGA

Product: NAD-dependent epimerase/dehydratase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MARYLVTGGAGFIGSHLVDALLQRGDEVRVFDNFSTGYEHNLAHCINDIELVRGDLRDAEAVSQAVAGCEVIFHEGALPS
VPRSVSDPLTTDAVNTGGSLHVLQAARQHGARRVVFAASSSVYGDTPILPKVETMAMSPKSPYAVSKMAAESYLKVFHHV
YGLETVGLRYFNVFGPRQDPTSQYSGVIARFMTLALQGEPYTMNGTGNQSRDFTYVANVVQANLLAASVPAAAGHVFNIA
CGLRISLNDVVAMLNKLVGKELPIIYSPARTGDVEHSLADISAARQILGFEPSVDIETGIARTLDWYRTQGA

Sequences:

>Translated_312_residues
MARYLVTGGAGFIGSHLVDALLQRGDEVRVFDNFSTGYEHNLAHCINDIELVRGDLRDAEAVSQAVAGCEVIFHEGALPS
VPRSVSDPLTTDAVNTGGSLHVLQAARQHGARRVVFAASSSVYGDTPILPKVETMAMSPKSPYAVSKMAAESYLKVFHHV
YGLETVGLRYFNVFGPRQDPTSQYSGVIARFMTLALQGEPYTMNGTGNQSRDFTYVANVVQANLLAASVPAAAGHVFNIA
CGLRISLNDVVAMLNKLVGKELPIIYSPARTGDVEHSLADISAARQILGFEPSVDIETGIARTLDWYRTQGA
>Mature_311_residues
ARYLVTGGAGFIGSHLVDALLQRGDEVRVFDNFSTGYEHNLAHCINDIELVRGDLRDAEAVSQAVAGCEVIFHEGALPSV
PRSVSDPLTTDAVNTGGSLHVLQAARQHGARRVVFAASSSVYGDTPILPKVETMAMSPKSPYAVSKMAAESYLKVFHHVY
GLETVGLRYFNVFGPRQDPTSQYSGVIARFMTLALQGEPYTMNGTGNQSRDFTYVANVVQANLLAASVPAAAGHVFNIAC
GLRISLNDVVAMLNKLVGKELPIIYSPARTGDVEHSLADISAARQILGFEPSVDIETGIARTLDWYRTQGA

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=315, Percent_Identity=33.015873015873, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI7657641, Length=321, Percent_Identity=26.791277258567, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI56237023, Length=337, Percent_Identity=27.0029673590504, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI56118217, Length=337, Percent_Identity=27.0029673590504, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI189083684, Length=337, Percent_Identity=27.0029673590504, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI193211614, Length=230, Percent_Identity=29.5652173913043, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI8393516, Length=230, Percent_Identity=29.5652173913043, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI4504031, Length=238, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786974, Length=332, Percent_Identity=29.5180722891566, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788353, Length=338, Percent_Identity=28.9940828402367, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994969, Length=341, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790049, Length=248, Percent_Identity=30.241935483871, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788589, Length=336, Percent_Identity=24.4047619047619, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788366, Length=349, Percent_Identity=24.6418338108883, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788365, Length=325, Percent_Identity=21.8461538461538, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539532, Length=317, Percent_Identity=30.2839116719243, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17568069, Length=327, Percent_Identity=26.2996941896024, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71982035, Length=337, Percent_Identity=28.1899109792285, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71982038, Length=339, Percent_Identity=28.6135693215339, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI115532424, Length=320, Percent_Identity=24.0625, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17507723, Length=334, Percent_Identity=24.8502994011976, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=327, Percent_Identity=24.7706422018349, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=327, Percent_Identity=24.7706422018349, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=327, Percent_Identity=24.7706422018349, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI133901786, Length=327, Percent_Identity=24.7706422018349, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=327, Percent_Identity=24.7706422018349, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=334, Percent_Identity=24.8502994011976, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356223, Length=317, Percent_Identity=32.4921135646688, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI19923002, Length=335, Percent_Identity=26.5671641791045, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 33528; Mature: 33397

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYLVTGGAGFIGSHLVDALLQRGDEVRVFDNFSTGYEHNLAHCINDIELVRGDLRDAE
CCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHH
AVSQAVAGCEVIFHEGALPSVPRSVSDPLTTDAVNTGGSLHVLQAARQHGARRVVFAASS
HHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHCCCEEEEEEECC
SVYGDTPILPKVETMAMSPKSPYAVSKMAAESYLKVFHHVYGLETVGLRYFNVFGPRQDP
CCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCCCC
TSQYSGVIARFMTLALQGEPYTMNGTGNQSRDFTYVANVVQANLLAASVPAAAGHVFNIA
HHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCEEEEE
CGLRISLNDVVAMLNKLVGKELPIIYSPARTGDVEHSLADISAARQILGFEPSVDIETGI
ECCEECHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
ARTLDWYRTQGA
HHHHHHHHCCCC
>Mature Secondary Structure 
ARYLVTGGAGFIGSHLVDALLQRGDEVRVFDNFSTGYEHNLAHCINDIELVRGDLRDAE
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHH
AVSQAVAGCEVIFHEGALPSVPRSVSDPLTTDAVNTGGSLHVLQAARQHGARRVVFAASS
HHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHCCCEEEEEEECC
SVYGDTPILPKVETMAMSPKSPYAVSKMAAESYLKVFHHVYGLETVGLRYFNVFGPRQDP
CCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCCCC
TSQYSGVIARFMTLALQGEPYTMNGTGNQSRDFTYVANVVQANLLAASVPAAAGHVFNIA
HHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCEEEEE
CGLRISLNDVVAMLNKLVGKELPIIYSPARTGDVEHSLADISAARQILGFEPSVDIETGI
ECCEECHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
ARTLDWYRTQGA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]