BacMap

Definition	Herpetosiphon aurantiacus ATCC 23779 chromosome, complete genome.
Accession	NC_009972
Length	6,346,587

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The map label for this gene is paaF [C]

Identifier: 159900754

GI number: 159900754

Start: 5409278

End: 5410063

Strand: Reverse

Name: paaF [C]

Synonym: Haur_4241

Alternate gene names: 159900754

Gene position: 5410063-5409278 (Counterclockwise)

Preceding gene: 159900758

Following gene: 159900753

Centisome position: 85.24

GC content: 52.16

Gene sequence:

>786_bases
ATGACTGACACGCTTCTGATTAGCAAAGCCGACAATGTAACCACTCTCACGATTAATCGCCCAGCGGTACGCAATGCCGT
CGATCAGCCAACCATGGATGCGCTGCGAGCGGAAATTGAGGCTTGCGAGCATGATGGCACTCGCTGTATTGTGATTGCTG
GTGCTGGTGGGGCATTTTCATCGGGAGCCGATATTATGGCGGCGCTCCAATCTGGTGGCACGGCAAATGATGTATATCGG
GTTTTAACTGAATCGTATGGCCCAACGCTGTTAGCAATTCGCCAATCGTCGTGGCCTGTGATTGCTGCCGTCGATGGCGT
AGCGGCTGGTATTGGCTCAGATTTGGCGTTGGCCTGCGATTTGCGCTTGGTTTCGAGCCGTGGGCGCTTCTCAGAAATTT
TTATCAAAGTTGGCTTAATTCCCGATGGCGGTGGAACCTATTCGTTGCCGCGCCTGATTGGCCTTGGGCGGGCGATGGAA
TTGATGTTTACCGGGGCGATGGTAGAGGCTGAACGAGCTTTGGCTTGGGGTATGGCCAATGCAGTCTTTGATGAAGCAAA
TTTTGCCAACGAGGTGCAAGCTTATGCTGCGACGATTGCGGCACAAGCTCCTTTGGCGTTAACCCGTGGTAAACGCGCAA
TGTTGGCCGCCCAAAATGATCAAAGTTTTGCCGATGCGCTACGTCGTGAAGCCGAATATCAACGCGAAATTTTTAATAGC
GAGGATGGCTTTGAAGGCTTTCAGGCCTTCTTGCAAAAACGCCCACCTGTGTGGAAAGGACGATAA

Upstream 100 bases:

>100_bases
TGCGTTGACGCTCTGCGTAAGATGGCCTATGATAAGGCCAAACCACTGCCTTTTCCTCGCTGTTTAACACCAAATTCTTT
AGCACAAAGGAAGATCGTCA

Downstream 100 bases:

>100_bases
CTCGTGCTAGCTAACCTGTATAGCTCAGATCAACAACGCTCACGCTTTATCTGGTTAATGGGCATTCAAGCGGTCTTGGT
GGTTGTGTTGCTGATCGCCT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTDTLLISKADNVTTLTINRPAVRNAVDQPTMDALRAEIEACEHDGTRCIVIAGAGGAFSSGADIMAALQSGGTANDVYR
VLTESYGPTLLAIRQSSWPVIAAVDGVAAGIGSDLALACDLRLVSSRGRFSEIFIKVGLIPDGGGTYSLPRLIGLGRAME
LMFTGAMVEAERALAWGMANAVFDEANFANEVQAYAATIAAQAPLALTRGKRAMLAAQNDQSFADALRREAEYQREIFNS
EDGFEGFQAFLQKRPPVWKGR

Sequences:

>Translated_261_residues
MTDTLLISKADNVTTLTINRPAVRNAVDQPTMDALRAEIEACEHDGTRCIVIAGAGGAFSSGADIMAALQSGGTANDVYR
VLTESYGPTLLAIRQSSWPVIAAVDGVAAGIGSDLALACDLRLVSSRGRFSEIFIKVGLIPDGGGTYSLPRLIGLGRAME
LMFTGAMVEAERALAWGMANAVFDEANFANEVQAYAATIAAQAPLALTRGKRAMLAAQNDQSFADALRREAEYQREIFNS
EDGFEGFQAFLQKRPPVWKGR
>Mature_260_residues
TDTLLISKADNVTTLTINRPAVRNAVDQPTMDALRAEIEACEHDGTRCIVIAGAGGAFSSGADIMAALQSGGTANDVYRV
LTESYGPTLLAIRQSSWPVIAAVDGVAAGIGSDLALACDLRLVSSRGRFSEIFIKVGLIPDGGGTYSLPRLIGLGRAMEL
MFTGAMVEAERALAWGMANAVFDEANFANEVQAYAATIAAQAPLALTRGKRAMLAAQNDQSFADALRREAEYQREIFNSE
DGFEGFQAFLQKRPPVWKGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=28.9156626506024, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=28.968253968254, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI70995211, Length=262, Percent_Identity=30.5343511450382, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI4502327, Length=259, Percent_Identity=30.5019305019305, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI25453481, Length=255, Percent_Identity=22.7450980392157, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI51538573, Length=255, Percent_Identity=22.7450980392157, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI45643119, Length=226, Percent_Identity=26.1061946902655, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI260275230, Length=226, Percent_Identity=26.1061946902655, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI260274832, Length=226, Percent_Identity=26.1061946902655, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI221136756, Length=257, Percent_Identity=22.1789883268482, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI221136753, Length=257, Percent_Identity=22.1789883268482, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI221307494, Length=257, Percent_Identity=22.1789883268482, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI4757966, Length=248, Percent_Identity=23.3870967741935, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI51538581, Length=248, Percent_Identity=23.3870967741935, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI4757968, Length=255, Percent_Identity=22.3529411764706, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI49355787, Length=255, Percent_Identity=22.3529411764706, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI116235487, Length=257, Percent_Identity=22.1789883268482, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI68989263, Length=217, Percent_Identity=29.9539170506912, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=27.8260869565217, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=27.9475982532751, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI157694520, Length=186, Percent_Identity=27.4193548387097, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=35.0943396226415, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787660, Length=267, Percent_Identity=36.3295880149813, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI221142681, Length=271, Percent_Identity=32.1033210332103, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=31.2, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=28.7878787878788, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788682, Length=186, Percent_Identity=31.1827956989247, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790281, Length=193, Percent_Identity=29.5336787564767, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=30.7692307692308, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=261, Percent_Identity=29.1187739463602, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=237, Percent_Identity=31.2236286919831, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=22.007722007722, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=259, Percent_Identity=26.2548262548263, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=257, Percent_Identity=25.2918287937743, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=233, Percent_Identity=27.0386266094421, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=233, Percent_Identity=27.0386266094421, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=233, Percent_Identity=27.0386266094421, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=230, Percent_Identity=25.6521739130435, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=26.0377358490566, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=240, Percent_Identity=23.3333333333333, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=210, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI20129971, Length=254, Percent_Identity=29.5275590551181, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24653477, Length=254, Percent_Identity=29.5275590551181, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24653139, Length=259, Percent_Identity=32.046332046332, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21357171, Length=258, Percent_Identity=30.2325581395349, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=25.2873563218391, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24654903, Length=212, Percent_Identity=24.5283018867925, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24650670, Length=219, Percent_Identity=28.310502283105, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571730, Length=153, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28571729, Length=153, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19920382, Length=220, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27809; Mature: 27678

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTLLISKADNVTTLTINRPAVRNAVDQPTMDALRAEIEACEHDGTRCIVIAGAGGAFS
CCCCEEEECCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
SGADIMAALQSGGTANDVYRVLTESYGPTLLAIRQSSWPVIAAVDGVAAGIGSDLALACD
CCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCHHHCCCCCEEEEEH
LRLVSSRGRFSEIFIKVGLIPDGGGTYSLPRLIGLGRAMELMFTGAMVEAERALAWGMAN
HHEECCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVFDEANFANEVQAYAATIAAQAPLALTRGKRAMLAAQNDQSFADALRREAEYQREIFNS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCC
EDGFEGFQAFLQKRPPVWKGR
CCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDTLLISKADNVTTLTINRPAVRNAVDQPTMDALRAEIEACEHDGTRCIVIAGAGGAFS
CCCEEEECCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
SGADIMAALQSGGTANDVYRVLTESYGPTLLAIRQSSWPVIAAVDGVAAGIGSDLALACD
CCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCHHHCCCCCEEEEEH
LRLVSSRGRFSEIFIKVGLIPDGGGTYSLPRLIGLGRAMELMFTGAMVEAERALAWGMAN
HHEECCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVFDEANFANEVQAYAATIAAQAPLALTRGKRAMLAAQNDQSFADALRREAEYQREIFNS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCC
EDGFEGFQAFLQKRPPVWKGR
CCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA