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The map label for this gene is pckA [H]

Identifier: 159900589

GI number: 159900589

Start: 5202352

End: 5203932

Strand: Direct

Name: pckA [H]

Synonym: Haur_4076

Alternate gene names: 159900589

Gene position: 5202352-5203932 (Clockwise)

Preceding gene: 159900588

Following gene: 159900598

Centisome position: 81.97

GC content: 54.08

Gene sequence:

>1581_bases
ATGACCGAACGAGCCGTATTAAGTGAAGGCGACACTGTAGGCGCTAATTTACATGTCAAGCAAGCCTATCGCAACTTAAC
GGTGCCGCAATTGGTGGAAGCTGCTCTCAAACGGGGCGAAGCCGTTTTATCGGCTACTGGGGCTGTCGTTGCCACCACGG
GCGCACGCACGGGTCGTTCTGCAGATGATAAGTTTGTGGTGGAAACACCAGCAGCCGCGTCAATGCACTGGACGAAATTC
CATAAAGCTATGAAGCCCGAAACCTATGCCACGATCAAGGCCAAGGCGTTGGCACACATGGCCGAACGTGAGATGTTTGT
TTTAGATGCTAGCGCTGGGGCTGATCCAGCGTATGCGTTGCCAATTCGCGTGGTGACCGAGTATGCTTGGCATAACTTGT
TCGCTAAGCAATTGTTCCGCGATGCGATCAGCAGCGATCAACAACCGCAATGGACGGTGCTCAACTTGCCAAGTTTGAAG
CTTGATCCAGCGGTTGATGGCTCGCGCTCAGAAGTTGCCGCCATGATCAATCTCGATGAAAAATTGATTTTGATTGTCGG
TACTGAATACGCTGGCGAGATCAAGAAATCGATCTTTACGGTATTGAACATGGTGCTGCCAAGCCAAGGCGTGATGCCAA
TGCACTGTTCAGCCAACATTGGCAGCAAGGGCGATGTAGCCTTGTTCTTCGGGCTTTCGGGCACGGGCAAAACCACGCTC
TCAGCCGACCCCGAACGGATTTTGATTGGCGATGATGAGCATGGTTGGAGCGCCAACGGCGTGTTCAACTTTGAAGGCGG
CTGCTATGCCAAGTGTATTCGCTTGCGCCGCGAATCGGAGCCAGAAATTTTCGACGCAATTCGCTATGGGGCGGTGCTCG
AAAACGTGGTGCTCAGCGATAGCCGCGATCCCAATTATGATGATGCGTCGTTGACCGAAAACACCCGCGCTGCCTATCCC
TTGGAATACATTCCCAACGTCAGCGAAACGGGTATGGGCGGCCAACCAGAAACGATCATCTTCTTGACCGCTGATGCCTT
TGGAGTTTTGCCGCCAATCGCCAAACTCAGCCCTGAACAAGCGATGTATCACTTCTTGTCGGGCTATACCGCCAAGCTGG
CTGGCACCGAAACGGGCGTTGGCTCAGAGCCACAAGCAACGTTTAGCACCTGCTTTGGCGCACCGTTTATGCCTTTGCAC
CCAACTGTGTATGCCGATTTGCTTGGCCAAAAAATGCGCGAACACAAAGTCCGTGTATTTTTGGTCAACACTGGCTGGAC
TGGTGGTTCGTTCGGGGTTGGCAAGCGCATGAGTTTGCGCGATACCCGCACGATGGTGCATGCCGCCTTGGCTGGCAAAC
TCGATGCTGTGGAAATGTGGCACGATGAGCGTTTCAATCTCGATGTGCCTGTGGCAATCGAAGGCGTTGATAACAGTGTG
CTGCAACCCCGCCAAACTTGGGCCGATGCCAGCGAATACGATCGGGTTGCCGATGACTTGGCCGCCCGCTTCCGCAAGAA
CTTCGAGCAATACGCCGAACGCGCTGGCGAAACCGTGGTAAACGCCGGCCCACAAGCGTAG

Upstream 100 bases:

>100_bases
ACGCTAGCTGGCTCACCAAGCGCAAGTTGAACGCGCTTGTGCTGGTTGTAGCCGCTACGGCAGCTTATTTTTATTTCTCT
TTATCTAAAGGAGCCATCTC

Downstream 100 bases:

>100_bases
GTCATGCCCTCACCCCCTAACCCCCTCTCCCGCAGGCGAGGCGAGGGGGAACCAGCCCACCATGAAAATTGGAACGCCCC
TCGCCCGCCGCAGTGGGAGA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MTERAVLSEGDTVGANLHVKQAYRNLTVPQLVEAALKRGEAVLSATGAVVATTGARTGRSADDKFVVETPAAASMHWTKF
HKAMKPETYATIKAKALAHMAEREMFVLDASAGADPAYALPIRVVTEYAWHNLFAKQLFRDAISSDQQPQWTVLNLPSLK
LDPAVDGSRSEVAAMINLDEKLILIVGTEYAGEIKKSIFTVLNMVLPSQGVMPMHCSANIGSKGDVALFFGLSGTGKTTL
SADPERILIGDDEHGWSANGVFNFEGGCYAKCIRLRRESEPEIFDAIRYGAVLENVVLSDSRDPNYDDASLTENTRAAYP
LEYIPNVSETGMGGQPETIIFLTADAFGVLPPIAKLSPEQAMYHFLSGYTAKLAGTETGVGSEPQATFSTCFGAPFMPLH
PTVYADLLGQKMREHKVRVFLVNTGWTGGSFGVGKRMSLRDTRTMVHAALAGKLDAVEMWHDERFNLDVPVAIEGVDNSV
LQPRQTWADASEYDRVADDLAARFRKNFEQYAERAGETVVNAGPQA

Sequences:

>Translated_526_residues
MTERAVLSEGDTVGANLHVKQAYRNLTVPQLVEAALKRGEAVLSATGAVVATTGARTGRSADDKFVVETPAAASMHWTKF
HKAMKPETYATIKAKALAHMAEREMFVLDASAGADPAYALPIRVVTEYAWHNLFAKQLFRDAISSDQQPQWTVLNLPSLK
LDPAVDGSRSEVAAMINLDEKLILIVGTEYAGEIKKSIFTVLNMVLPSQGVMPMHCSANIGSKGDVALFFGLSGTGKTTL
SADPERILIGDDEHGWSANGVFNFEGGCYAKCIRLRRESEPEIFDAIRYGAVLENVVLSDSRDPNYDDASLTENTRAAYP
LEYIPNVSETGMGGQPETIIFLTADAFGVLPPIAKLSPEQAMYHFLSGYTAKLAGTETGVGSEPQATFSTCFGAPFMPLH
PTVYADLLGQKMREHKVRVFLVNTGWTGGSFGVGKRMSLRDTRTMVHAALAGKLDAVEMWHDERFNLDVPVAIEGVDNSV
LQPRQTWADASEYDRVADDLAARFRKNFEQYAERAGETVVNAGPQA
>Mature_525_residues
TERAVLSEGDTVGANLHVKQAYRNLTVPQLVEAALKRGEAVLSATGAVVATTGARTGRSADDKFVVETPAAASMHWTKFH
KAMKPETYATIKAKALAHMAEREMFVLDASAGADPAYALPIRVVTEYAWHNLFAKQLFRDAISSDQQPQWTVLNLPSLKL
DPAVDGSRSEVAAMINLDEKLILIVGTEYAGEIKKSIFTVLNMVLPSQGVMPMHCSANIGSKGDVALFFGLSGTGKTTLS
ADPERILIGDDEHGWSANGVFNFEGGCYAKCIRLRRESEPEIFDAIRYGAVLENVVLSDSRDPNYDDASLTENTRAAYPL
EYIPNVSETGMGGQPETIIFLTADAFGVLPPIAKLSPEQAMYHFLSGYTAKLAGTETGVGSEPQATFSTCFGAPFMPLHP
TVYADLLGQKMREHKVRVFLVNTGWTGGSFGVGKRMSLRDTRTMVHAALAGKLDAVEMWHDERFNLDVPVAIEGVDNSVL
QPRQTWADASEYDRVADDLAARFRKNFEQYAERAGETVVNAGPQA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=502, Percent_Identity=46.4143426294821, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322950, Length=514, Percent_Identity=45.9143968871595, Blast_Score=446, Evalue=1e-126,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 57205; Mature: 57074

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERAVLSEGDTVGANLHVKQAYRNLTVPQLVEAALKRGEAVLSATGAVVATTGARTGRS
CCCCCCCCCCCCCCCEEEHHHHHHCCCHHHHHHHHHHCCCEEEEECCEEEEECCCCCCCC
ADDKFVVETPAAASMHWTKFHKAMKPETYATIKAKALAHMAEREMFVLDASAGADPAYAL
CCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEC
PIRVVTEYAWHNLFAKQLFRDAISSDQQPQWTVLNLPSLKLDPAVDGSRSEVAAMINLDE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECCCCCCCHHHEEEEEECCC
KLILIVGTEYAGEIKKSIFTVLNMVLPSQGVMPMHCSANIGSKGDVALFFGLSGTGKTTL
EEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEEE
SADPERILIGDDEHGWSANGVFNFEGGCYAKCIRLRRESEPEIFDAIRYGAVLENVVLSD
CCCCCEEEECCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
SRDPNYDDASLTENTRAAYPLEYIPNVSETGMGGQPETIIFLTADAFGVLPPIAKLSPEQ
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHCCHHH
AMYHFLSGYTAKLAGTETGVGSEPQATFSTCFGAPFMPLHPTVYADLLGQKMREHKVRVF
HHHHHHCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
LVNTGWTGGSFGVGKRMSLRDTRTMVHAALAGKLDAVEMWHDERFNLDVPVAIEGVDNSV
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCCCC
LQPRQTWADASEYDRVADDLAARFRKNFEQYAERAGETVVNAGPQA
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
>Mature Secondary Structure 
TERAVLSEGDTVGANLHVKQAYRNLTVPQLVEAALKRGEAVLSATGAVVATTGARTGRS
CCCCCCCCCCCCCCEEEHHHHHHCCCHHHHHHHHHHCCCEEEEECCEEEEECCCCCCCC
ADDKFVVETPAAASMHWTKFHKAMKPETYATIKAKALAHMAEREMFVLDASAGADPAYAL
CCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEC
PIRVVTEYAWHNLFAKQLFRDAISSDQQPQWTVLNLPSLKLDPAVDGSRSEVAAMINLDE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECCCCCCCHHHEEEEEECCC
KLILIVGTEYAGEIKKSIFTVLNMVLPSQGVMPMHCSANIGSKGDVALFFGLSGTGKTTL
EEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEEE
SADPERILIGDDEHGWSANGVFNFEGGCYAKCIRLRRESEPEIFDAIRYGAVLENVVLSD
CCCCCEEEECCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
SRDPNYDDASLTENTRAAYPLEYIPNVSETGMGGQPETIIFLTADAFGVLPPIAKLSPEQ
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHCCHHH
AMYHFLSGYTAKLAGTETGVGSEPQATFSTCFGAPFMPLHPTVYADLLGQKMREHKVRVF
HHHHHHCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
LVNTGWTGGSFGVGKRMSLRDTRTMVHAALAGKLDAVEMWHDERFNLDVPVAIEGVDNSV
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCCCC
LQPRQTWADASEYDRVADDLAARFRKNFEQYAERAGETVVNAGPQA
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA