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The map label for this gene is appA [H]

Identifier: 159900531

GI number: 159900531

Start: 5125407

End: 5127068

Strand: Reverse

Name: appA [H]

Synonym: Haur_4018

Alternate gene names: 159900531

Gene position: 5127068-5125407 (Counterclockwise)

Preceding gene: 159900532

Following gene: 159900530

Centisome position: 80.78

GC content: 51.32

Gene sequence:

>1662_bases
ATGATGCGAATATTGACCCTAGGATTATTGGCTGTTTTGCTCACAGCATGTAGCCTCGGCTCCACACCAGCACCCACCAA
CGAGCCAACCACACCGCCAATTCAGGTTCCGCAAGGTGGTACGTTAACCATTCGCACGGCCCAAGATATTGCAGCTTTGC
ACCCGTGGAAACCAACCTGCCACGAAGAAGCTCAACTTTTAGGCCTGCTCTATCGTGGTTTAACTAAGCTTGATCAAAGC
CTCGCTCCGCAACCTGATGTTGCTACAAGCTGGCAAAGCGATAGTATCGGCCAAACCCTGACCATGACCTTGCGCAGCGA
TATTCGTTGGCATGATGATACGACCTTGACTGCTGCTGATGCGGCTTGGACGATCAGCGCCATGCAAAGCATCAGCCCAA
CCACGCCATTATTGACCGATCTTCAGGGCTTGGTGCGCAAGGTTACTGCACCCGATGACACAACTTTGGTCATTTCGTTG
CGCGAACCCTATGCGCCATTGCTCTCGGCCTTGAGCATGCCAATTTTGCCCAAACATGTGTTTGAGCAATTAAGCCCAGT
TGAGCTTGATCAGCTTAATCTTTTGACGCAGCCAATTGGTAGCGGCCCGTTTATGTTCGAGCAACGGACTGCTGGCTCAG
CAATTAGCTTAATTCGCAATAGCAACTATATCGATGGCGTGCCCTATCTCGATCGGGTGGCCTTTGTGGTTGCCCCCGAT
CCGCAAGTGGCTCGCCAAGCAGTGCGCGATGGAGATTTATTGGCAGCAGAATTGCCATGGGCACAAAGCCAAGGCTTAGG
GCCAACAGTTGGCATAGGTAGTTATCCTGAAAATGGTTTTTATTATTTGGCCTTTAATATGCGTGATGGTCGCATCTTCA
GCGATCAGCGAGTGCGCCAAGCGCTGGCATTAAGCCTCGATCTCAATACAATTGTCGAAACCGCTGGCCCAGCCGCTCAA
GCAATTTTGAGCGATCATTTGCCTGGCACATGGGTTGCGCCAACTGGTGAGTTGCCCAAACGCAACTTAGATCAAGCTCG
CGAATTACTGGATCAAGCAGGCTGGGTCTTGCCCGAAGGTGCGACAATTCGCGCCTCGAACGGGATTACGCTGTCGATGG
CGCTGTTTGTGCGGGGCGATGACCAACGCCGCATCGAGGTTGCCGAACGGATCGCCGCTGCTACACGTCCGGTTGGCTTC
AATATTGTGGTTACGCCAGCCGATTTCGAGAGCGTGATTCGCTCTAAATTGGTAACACCCTTTGATTTTGATTTGGCTTT
GATGAGTTGGGGCAATAGTCGAGTTGGTGGTTCGCCCTCGTATACCGCCTACGATCCTGATAATTTCTCGTTGTTTCATT
CGAGCCAAATTTACCAAGGGGTTGCCGATGGACGGCCTGGCCTGCGCAATTATGGTGCGTTTCAAAACACCAGTTTCGAT
AATTTATCGACGGCAGCGCGGGCACTTTACGCAACTGAACGCCGCCGCGAACTCTATCAACAAACCAACACAATCATTCA
AACCGAATATCCGTATGTGTTTCTGTGGGCCGATCGGATTCCGGTCGCCTTAGCCAAACAGGTGCGTTCAACTCAAGGCG
AAATTCGGTTGGATACGGCCAATTGGCTGTATGATGTTCAACATTGGTATCTTGAGCAATAA

Upstream 100 bases:

>100_bases
GTGGCATGGGTCATCACCTATATGGGTTTGGGCTATGCGATGGGCTTGTTTAGTTGATTCGAGTGGGCGTTCTAGCGCTC
TTGTGGAGTAATAAGCTTCT

Downstream 100 bases:

>100_bases
TCATGGATGCTGCGGCGATTGCCAAATTCAGCGAACAACTGATTGCCACCGCGATTGCCAGTGTTGATCCTCAGCGGGCA
GTTTTGCAGGCCTTGGATTG

Product: extracellular solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MMRILTLGLLAVLLTACSLGSTPAPTNEPTTPPIQVPQGGTLTIRTAQDIAALHPWKPTCHEEAQLLGLLYRGLTKLDQS
LAPQPDVATSWQSDSIGQTLTMTLRSDIRWHDDTTLTAADAAWTISAMQSISPTTPLLTDLQGLVRKVTAPDDTTLVISL
REPYAPLLSALSMPILPKHVFEQLSPVELDQLNLLTQPIGSGPFMFEQRTAGSAISLIRNSNYIDGVPYLDRVAFVVAPD
PQVARQAVRDGDLLAAELPWAQSQGLGPTVGIGSYPENGFYYLAFNMRDGRIFSDQRVRQALALSLDLNTIVETAGPAAQ
AILSDHLPGTWVAPTGELPKRNLDQARELLDQAGWVLPEGATIRASNGITLSMALFVRGDDQRRIEVAERIAAATRPVGF
NIVVTPADFESVIRSKLVTPFDFDLALMSWGNSRVGGSPSYTAYDPDNFSLFHSSQIYQGVADGRPGLRNYGAFQNTSFD
NLSTAARALYATERRRELYQQTNTIIQTEYPYVFLWADRIPVALAKQVRSTQGEIRLDTANWLYDVQHWYLEQ

Sequences:

>Translated_553_residues
MMRILTLGLLAVLLTACSLGSTPAPTNEPTTPPIQVPQGGTLTIRTAQDIAALHPWKPTCHEEAQLLGLLYRGLTKLDQS
LAPQPDVATSWQSDSIGQTLTMTLRSDIRWHDDTTLTAADAAWTISAMQSISPTTPLLTDLQGLVRKVTAPDDTTLVISL
REPYAPLLSALSMPILPKHVFEQLSPVELDQLNLLTQPIGSGPFMFEQRTAGSAISLIRNSNYIDGVPYLDRVAFVVAPD
PQVARQAVRDGDLLAAELPWAQSQGLGPTVGIGSYPENGFYYLAFNMRDGRIFSDQRVRQALALSLDLNTIVETAGPAAQ
AILSDHLPGTWVAPTGELPKRNLDQARELLDQAGWVLPEGATIRASNGITLSMALFVRGDDQRRIEVAERIAAATRPVGF
NIVVTPADFESVIRSKLVTPFDFDLALMSWGNSRVGGSPSYTAYDPDNFSLFHSSQIYQGVADGRPGLRNYGAFQNTSFD
NLSTAARALYATERRRELYQQTNTIIQTEYPYVFLWADRIPVALAKQVRSTQGEIRLDTANWLYDVQHWYLEQ
>Mature_553_residues
MMRILTLGLLAVLLTACSLGSTPAPTNEPTTPPIQVPQGGTLTIRTAQDIAALHPWKPTCHEEAQLLGLLYRGLTKLDQS
LAPQPDVATSWQSDSIGQTLTMTLRSDIRWHDDTTLTAADAAWTISAMQSISPTTPLLTDLQGLVRKVTAPDDTTLVISL
REPYAPLLSALSMPILPKHVFEQLSPVELDQLNLLTQPIGSGPFMFEQRTAGSAISLIRNSNYIDGVPYLDRVAFVVAPD
PQVARQAVRDGDLLAAELPWAQSQGLGPTVGIGSYPENGFYYLAFNMRDGRIFSDQRVRQALALSLDLNTIVETAGPAAQ
AILSDHLPGTWVAPTGELPKRNLDQARELLDQAGWVLPEGATIRASNGITLSMALFVRGDDQRRIEVAERIAAATRPVGF
NIVVTPADFESVIRSKLVTPFDFDLALMSWGNSRVGGSPSYTAYDPDNFSLFHSSQIYQGVADGRPGLRNYGAFQNTSFD
NLSTAARALYATERRRELYQQTNTIIQTEYPYVFLWADRIPVALAKQVRSTQGEIRLDTANWLYDVQHWYLEQ

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=431, Percent_Identity=25.754060324826, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1789966, Length=477, Percent_Identity=23.0607966457023, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787762, Length=459, Percent_Identity=23.3115468409586, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI1787052, Length=371, Percent_Identity=20.7547169811321, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60763; Mature: 60763

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRILTLGLLAVLLTACSLGSTPAPTNEPTTPPIQVPQGGTLTIRTAQDIAALHPWKPTC
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCEEEEEEHHHHHHCCCCCCCH
HEEAQLLGLLYRGLTKLDQSLAPQPDVATSWQSDSIGQTLTMTLRSDIRWHDDTTLTAAD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEECCCCEEEECC
AAWTISAMQSISPTTPLLTDLQGLVRKVTAPDDTTLVISLREPYAPLLSALSMPILPKHV
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCHHH
FEQLSPVELDQLNLLTQPIGSGPFMFEQRTAGSAISLIRNSNYIDGVPYLDRVAFVVAPD
HHHCCCCCHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCHHHHEEEEECCC
PQVARQAVRDGDLLAAELPWAQSQGLGPTVGIGSYPENGFYYLAFNMRDGRIFSDQRVRQ
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECHHHHHH
ALALSLDLNTIVETAGPAAQAILSDHLPGTWVAPTGELPKRNLDQARELLDQAGWVLPEG
HHHHHCCHHHHHHCCCHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCC
ATIRASNGITLSMALFVRGDDQRRIEVAERIAAATRPVGFNIVVTPADFESVIRSKLVTP
CEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCC
FDFDLALMSWGNSRVGGSPSYTAYDPDNFSLFHSSQIYQGVADGRPGLRNYGAFQNTSFD
CCCCCCEEECCCCCCCCCCCCCEECCCCCEEEECHHHHHHHHCCCCCCHHCCCCCCCCCC
NLSTAARALYATERRRELYQQTNTIIQTEYPYVFLWADRIPVALAKQVRSTQGEIRLDTA
HHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCHHHHHHHHCCCCCEEEEHH
NWLYDVQHWYLEQ
HHHHHHHHHHCCC
>Mature Secondary Structure
MMRILTLGLLAVLLTACSLGSTPAPTNEPTTPPIQVPQGGTLTIRTAQDIAALHPWKPTC
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCEEEEEEHHHHHHCCCCCCCH
HEEAQLLGLLYRGLTKLDQSLAPQPDVATSWQSDSIGQTLTMTLRSDIRWHDDTTLTAAD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEECCCCEEEECC
AAWTISAMQSISPTTPLLTDLQGLVRKVTAPDDTTLVISLREPYAPLLSALSMPILPKHV
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCHHH
FEQLSPVELDQLNLLTQPIGSGPFMFEQRTAGSAISLIRNSNYIDGVPYLDRVAFVVAPD
HHHCCCCCHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCHHHHEEEEECCC
PQVARQAVRDGDLLAAELPWAQSQGLGPTVGIGSYPENGFYYLAFNMRDGRIFSDQRVRQ
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECHHHHHH
ALALSLDLNTIVETAGPAAQAILSDHLPGTWVAPTGELPKRNLDQARELLDQAGWVLPEG
HHHHHCCHHHHHHCCCHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCC
ATIRASNGITLSMALFVRGDDQRRIEVAERIAAATRPVGFNIVVTPADFESVIRSKLVTP
CEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCC
FDFDLALMSWGNSRVGGSPSYTAYDPDNFSLFHSSQIYQGVADGRPGLRNYGAFQNTSFD
CCCCCCEEECCCCCCCCCCCCCEECCCCCEEEECHHHHHHHHCCCCCCHHCCCCCCCCCC
NLSTAARALYATERRRELYQQTNTIIQTEYPYVFLWADRIPVALAKQVRSTQGEIRLDTA
HHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCHHHHHHHHCCCCCEEEEHH
NWLYDVQHWYLEQ
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]