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The map label for this gene is pnp

Identifier: 159900395

GI number: 159900395

Start: 4872013

End: 4874346

Strand: Reverse

Name: pnp

Synonym: Haur_3880

Alternate gene names: 159900395

Gene position: 4874346-4872013 (Counterclockwise)

Preceding gene: 159900396

Following gene: 159900391

Centisome position: 76.8

GC content: 54.03

Gene sequence:

>2334_bases
TTGAGTGAACAACAAGTTCATAGCGTAACTGTCGAGCTGGCGGGTCGCCCGTTAACAATTGAAGCAGGTCGGGTTGCGCA
GTTGGCCAATGGAGCAGTTTTGGTGCGATATGGCGATACCGTTTTGCTCGCCGCTGTCACCGCGTCAGCCGAACCACGCG
ATGGGATTGATTTTTTCCCACTGACGGTTGACTATGAAGAAAAGATGTATGCCGCAGGTAAAATCCCCGGTTCGTTCTTC
AAACGTGAAGGCCGCCCAACCGAAACTGCTATTTTAACCTCGCGCTTGACTGACCGTCCATTGCGACCTTTGTTTCCCAA
AGGTTATTACAATGACGTGCAGGTCTCGATTACGACGCTTTCAACCGATCAAGTTAATGATCCTGGGCCGTTGGCAATTA
TTGGTGCTTCGGCAGCCTTGGTGATTTCCGATATTCCGTTTGCTGCGCCAGTTGGTGCGGTGCAAATGGGTCATATCGAT
GGCGTTTTTGCAGTCAACCCAGTTATGGGCTTGATGGAAAATAGCCGCCTCGATTTGGTGGTTGCTGGTACCAGCGAAGC
CGTAATGATGGTCGAAGCGAGCGCCTACGAATTGACCGAAGCTGAAATGCTCGAAGCGGTCAAACGCGGCCACGAAGCCA
TGCAAGCGGTGATTCAAGCCCAACTTGAGTTGGCCGAAAAATGTGGCAAGCCCAAAAAAGAATTTGTGGCTCCGGTCGTC
GATACTAGCCTCCAAGACGAAATCAAAGCTTGGATGGGCAATCGCTTCTTGCAAGCCTTGAACAATAGCGATAAAGCTGC
CCGTGAAGCTGCTACCGATAGCTTGCGCACCGATGTGTTGGAGCAATTCAGCGCTGGTGTAGCTGAAGAAGAATTGGCCG
CTCGGGTCAAGGCAGTAACGAAAAACTACGATGCCTTGGTCAAAGATGAAGTTCGTGCTTCGATTCTTGAGCAAGGGATT
CGCGTCGATGGCCGCAAGGTCAACGAAATTCGGCCTATTGCGGTCGATGTTGGGGTTTTGCCACGCGTTCACGGCTCGGG
CTTGTTTACCCGTGGTCAAACCCAAGTGCTCACTGTAGCAACGCTTGGATCGCCTGGCGATGAGCAACGGCTCGACGATT
TGGGCATCGAAACCACCCGTCACTACATGCACCACTACAACTTCCCAGCCTTCTCGACTGGCGAAGCTCGCCCTTCACGC
GGCCCACGTCGCCGTGATATTGGCCATGGCAAGTTGGCCGAACGCGCTTTGGTACCAGTCCTGCCTGAAAAAGACAAGTT
CCCCTACACCATGCGCTTGGTCTCGGAAGTCTTGGCCTCGAACGGTTCATCGTCAATGGCTTCGGTGTGTGGTTCATCGC
TGGCCTTGATGGATGCTGGTGTGCCAATCAAAGCTCCAGTTGCTGGCGTGGCAATGGGCTTGATCACTGGCAAAGAAGCT
GGTCAGTTCAGCGTTTTGACCGACATCCAAGGCTTGGAAGATGCTTTGGGCGATATGGACTTCAAGGTTGCTGGCACGAC
CGCTGGCATCACCGCGCTGCAAATGGATATCAAAACCACGGGCATTACCTATGAAATTATGCAGCAAGCATTTGAGCAAG
CTCGCCAAGGGCGTGTGTTCATTCTCGATAAGATGAACGAAATTATCAACACTGCCCGCAACGATATTTCCCAATTTGCG
CCACGGATTATCACCCTGCAAATCGATCCATCGAAGATTGGGGCATTGATTGGGCCTGGTGGTAAGACGATTCGCTCGAT
TATCGAGCAAACTGGTGCTCAAATTGATGTTGAGGATGATGGCCGCGTATTCGTGACCACGCCCGATGCCGATGGCGCAC
GCATGGCTCAATCGTTGATCGAAGGCTTGACCCGCGAAGCCAAAGTTGGCGAAATCTTTACTGGTAAAGTTGTGCGGATT
ATGCCGTATGGCGCGTTCGTCAACGTGTTGCCAGGCAAAGACGGCATGGTTCACGTTTCCGAGCTTGATGAAAAGCGGGT
CGAAAACGTTGAAGATGTGGTCAAAATTGGCGACGAATTGACCGTGATGGTGATCGATGTTGAGCCAGGCACTGGCAAAT
TGAGCCTTTCACGTCGCGCAATTCTGACTGGCGAAACCGCTGAGCAACGCAAGAGCAGCAGTAGCAAAGGCGGCCCTCGC
GGTGGCGGTGGTGGCGGCGACCGTGGCCCACGCCCAGGTGGCGATCGTGGCCCACGCCCTGAAGGTGATCGCGGCCCACG
CCCCGAAGGTGACCGCCCACGTGAAGGCGGCGACCGTGGCCCACGCCCAGGTAACGGTGGCAATGACCGCCGTGGTGGTG
GCTTCCGCGGCTAA

Upstream 100 bases:

>100_bases
GGAATTGGCGCGTGAACAGTGACAGGGAGTTGCTGCGCAGGCTCCAGTGCCTAACGCTCCACATCGTGAAGCACCCAAGT
GGGGTGTTTGGAGGCACATT

Downstream 100 bases:

>100_bases
TAGGTTTTGATCTACGAAGGACACGAAGCACATGAAAGATAAGGCTCTAGGCTTTGGGCTATCGGAACATGGGTTGGTAT
TTCTAGCCAATCCCCGATAG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 777; Mature: 776

Protein sequence:

>777_residues
MSEQQVHSVTVELAGRPLTIEAGRVAQLANGAVLVRYGDTVLLAAVTASAEPRDGIDFFPLTVDYEEKMYAAGKIPGSFF
KREGRPTETAILTSRLTDRPLRPLFPKGYYNDVQVSITTLSTDQVNDPGPLAIIGASAALVISDIPFAAPVGAVQMGHID
GVFAVNPVMGLMENSRLDLVVAGTSEAVMMVEASAYELTEAEMLEAVKRGHEAMQAVIQAQLELAEKCGKPKKEFVAPVV
DTSLQDEIKAWMGNRFLQALNNSDKAAREAATDSLRTDVLEQFSAGVAEEELAARVKAVTKNYDALVKDEVRASILEQGI
RVDGRKVNEIRPIAVDVGVLPRVHGSGLFTRGQTQVLTVATLGSPGDEQRLDDLGIETTRHYMHHYNFPAFSTGEARPSR
GPRRRDIGHGKLAERALVPVLPEKDKFPYTMRLVSEVLASNGSSSMASVCGSSLALMDAGVPIKAPVAGVAMGLITGKEA
GQFSVLTDIQGLEDALGDMDFKVAGTTAGITALQMDIKTTGITYEIMQQAFEQARQGRVFILDKMNEIINTARNDISQFA
PRIITLQIDPSKIGALIGPGGKTIRSIIEQTGAQIDVEDDGRVFVTTPDADGARMAQSLIEGLTREAKVGEIFTGKVVRI
MPYGAFVNVLPGKDGMVHVSELDEKRVENVEDVVKIGDELTVMVIDVEPGTGKLSLSRRAILTGETAEQRKSSSSKGGPR
GGGGGGDRGPRPGGDRGPRPEGDRGPRPEGDRPREGGDRGPRPGNGGNDRRGGGFRG

Sequences:

>Translated_777_residues
MSEQQVHSVTVELAGRPLTIEAGRVAQLANGAVLVRYGDTVLLAAVTASAEPRDGIDFFPLTVDYEEKMYAAGKIPGSFF
KREGRPTETAILTSRLTDRPLRPLFPKGYYNDVQVSITTLSTDQVNDPGPLAIIGASAALVISDIPFAAPVGAVQMGHID
GVFAVNPVMGLMENSRLDLVVAGTSEAVMMVEASAYELTEAEMLEAVKRGHEAMQAVIQAQLELAEKCGKPKKEFVAPVV
DTSLQDEIKAWMGNRFLQALNNSDKAAREAATDSLRTDVLEQFSAGVAEEELAARVKAVTKNYDALVKDEVRASILEQGI
RVDGRKVNEIRPIAVDVGVLPRVHGSGLFTRGQTQVLTVATLGSPGDEQRLDDLGIETTRHYMHHYNFPAFSTGEARPSR
GPRRRDIGHGKLAERALVPVLPEKDKFPYTMRLVSEVLASNGSSSMASVCGSSLALMDAGVPIKAPVAGVAMGLITGKEA
GQFSVLTDIQGLEDALGDMDFKVAGTTAGITALQMDIKTTGITYEIMQQAFEQARQGRVFILDKMNEIINTARNDISQFA
PRIITLQIDPSKIGALIGPGGKTIRSIIEQTGAQIDVEDDGRVFVTTPDADGARMAQSLIEGLTREAKVGEIFTGKVVRI
MPYGAFVNVLPGKDGMVHVSELDEKRVENVEDVVKIGDELTVMVIDVEPGTGKLSLSRRAILTGETAEQRKSSSSKGGPR
GGGGGGDRGPRPGGDRGPRPEGDRGPRPEGDRPREGGDRGPRPGNGGNDRRGGGFRG
>Mature_776_residues
SEQQVHSVTVELAGRPLTIEAGRVAQLANGAVLVRYGDTVLLAAVTASAEPRDGIDFFPLTVDYEEKMYAAGKIPGSFFK
REGRPTETAILTSRLTDRPLRPLFPKGYYNDVQVSITTLSTDQVNDPGPLAIIGASAALVISDIPFAAPVGAVQMGHIDG
VFAVNPVMGLMENSRLDLVVAGTSEAVMMVEASAYELTEAEMLEAVKRGHEAMQAVIQAQLELAEKCGKPKKEFVAPVVD
TSLQDEIKAWMGNRFLQALNNSDKAAREAATDSLRTDVLEQFSAGVAEEELAARVKAVTKNYDALVKDEVRASILEQGIR
VDGRKVNEIRPIAVDVGVLPRVHGSGLFTRGQTQVLTVATLGSPGDEQRLDDLGIETTRHYMHHYNFPAFSTGEARPSRG
PRRRDIGHGKLAERALVPVLPEKDKFPYTMRLVSEVLASNGSSSMASVCGSSLALMDAGVPIKAPVAGVAMGLITGKEAG
QFSVLTDIQGLEDALGDMDFKVAGTTAGITALQMDIKTTGITYEIMQQAFEQARQGRVFILDKMNEIINTARNDISQFAP
RIITLQIDPSKIGALIGPGGKTIRSIIEQTGAQIDVEDDGRVFVTTPDADGARMAQSLIEGLTREAKVGEIFTGKVVRIM
PYGAFVNVLPGKDGMVHVSELDEKRVENVEDVVKIGDELTVMVIDVEPGTGKLSLSRRAILTGETAEQRKSSSSKGGPRG
GGGGGDRGPRPGGDRGPRPEGDRGPRPEGDRPREGGDRGPRPGNGGNDRRGGGFRG

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=727, Percent_Identity=37.0013755158184, Blast_Score=444, Evalue=1e-124,
Organism=Escherichia coli, GI145693187, Length=683, Percent_Identity=49.194729136164, Blast_Score=630, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=727, Percent_Identity=33.0123796423659, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI281362905, Length=717, Percent_Identity=37.5174337517434, Blast_Score=457, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651641, Length=717, Percent_Identity=37.5174337517434, Blast_Score=457, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651643, Length=717, Percent_Identity=37.5174337517434, Blast_Score=457, Evalue=1e-128,
Organism=Drosophila melanogaster, GI161079377, Length=654, Percent_Identity=37.0030581039755, Blast_Score=412, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_HERA2 (A9B8G1)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001546642.1
- ProteinModelPortal:   A9B8G1
- SMR:   A9B8G1
- GeneID:   5735729
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_3880
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   HAUR316274:HAUR_3880-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 83009; Mature: 82878

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQQVHSVTVELAGRPLTIEAGRVAQLANGAVLVRYGDTVLLAAVTASAEPRDGIDFFP
CCCCCEEEEEEEECCCEEEEECCCHHHHCCCEEEEEECCEEEEEEEECCCCCCCCCEEEE
LTVDYEEKMYAAGKIPGSFFKREGRPTETAILTSRLTDRPLRPLFPKGYYNDVQVSITTL
EEECHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEE
STDQVNDPGPLAIIGASAALVISDIPFAAPVGAVQMGHIDGVFAVNPVMGLMENSRLDLV
ECCCCCCCCCEEEECCCEEEEEECCCCCCCCCCEEECCCCCEEEHHHHHHHHCCCCEEEE
VAGTSEAVMMVEASAYELTEAEMLEAVKRGHEAMQAVIQAQLELAEKCGKPKKEFVAPVV
EECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DTSLQDEIKAWMGNRFLQALNNSDKAAREAATDSLRTDVLEQFSAGVAEEELAARVKAVT
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KNYDALVKDEVRASILEQGIRVDGRKVNEIRPIAVDVGVLPRVHGSGLFTRGQTQVLTVA
HCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCEEEEEE
TLGSPGDEQRLDDLGIETTRHYMHHYNFPAFSTGEARPSRGPRRRDIGHGKLAERALVPV
ECCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
LPEKDKFPYTMRLVSEVLASNGSSSMASVCGSSLALMDAGVPIKAPVAGVAMGLITGKEA
CCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHEECCCC
GQFSVLTDIQGLEDALGDMDFKVAGTTAGITALQMDIKTTGITYEIMQQAFEQARQGRVF
CCEEEHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCEE
ILDKMNEIINTARNDISQFAPRIITLQIDPSKIGALIGPGGKTIRSIIEQTGAQIDVEDD
EEHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHCEEECCCHHHHHHHHHHCCCEEEECCC
GRVFVTTPDADGARMAQSLIEGLTREAKVGEIFTGKVVRIMPYGAFVNVLPGKDGMVHVS
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEECCCEEEEEECCCEEEEECCCCCEEEHH
ELDEKRVENVEDVVKIGDELTVMVIDVEPGTGKLSLSRRAILTGETAEQRKSSSSKGGPR
HHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEECCEEEECCCCHHHHHCCCCCCCCC
GGGGGGDRGPRPGGDRGPRPEGDRGPRPEGDRPREGGDRGPRPGNGGNDRRGGGFRG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEQQVHSVTVELAGRPLTIEAGRVAQLANGAVLVRYGDTVLLAAVTASAEPRDGIDFFP
CCCCEEEEEEEECCCEEEEECCCHHHHCCCEEEEEECCEEEEEEEECCCCCCCCCEEEE
LTVDYEEKMYAAGKIPGSFFKREGRPTETAILTSRLTDRPLRPLFPKGYYNDVQVSITTL
EEECHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEE
STDQVNDPGPLAIIGASAALVISDIPFAAPVGAVQMGHIDGVFAVNPVMGLMENSRLDLV
ECCCCCCCCCEEEECCCEEEEEECCCCCCCCCCEEECCCCCEEEHHHHHHHHCCCCEEEE
VAGTSEAVMMVEASAYELTEAEMLEAVKRGHEAMQAVIQAQLELAEKCGKPKKEFVAPVV
EECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DTSLQDEIKAWMGNRFLQALNNSDKAAREAATDSLRTDVLEQFSAGVAEEELAARVKAVT
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KNYDALVKDEVRASILEQGIRVDGRKVNEIRPIAVDVGVLPRVHGSGLFTRGQTQVLTVA
HCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCEEEEEE
TLGSPGDEQRLDDLGIETTRHYMHHYNFPAFSTGEARPSRGPRRRDIGHGKLAERALVPV
ECCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
LPEKDKFPYTMRLVSEVLASNGSSSMASVCGSSLALMDAGVPIKAPVAGVAMGLITGKEA
CCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHEECCCC
GQFSVLTDIQGLEDALGDMDFKVAGTTAGITALQMDIKTTGITYEIMQQAFEQARQGRVF
CCEEEHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCEE
ILDKMNEIINTARNDISQFAPRIITLQIDPSKIGALIGPGGKTIRSIIEQTGAQIDVEDD
EEHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHCEEECCCHHHHHHHHHHCCCEEEECCC
GRVFVTTPDADGARMAQSLIEGLTREAKVGEIFTGKVVRIMPYGAFVNVLPGKDGMVHVS
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEECCCEEEEEECCCEEEEECCCCCEEEHH
ELDEKRVENVEDVVKIGDELTVMVIDVEPGTGKLSLSRRAILTGETAEQRKSSSSKGGPR
HHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEECCEEEECCCCHHHHHCCCCCCCCC
GGGGGGDRGPRPGGDRGPRPEGDRGPRPEGDRPREGGDRGPRPGNGGNDRRGGGFRG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA